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EBI DbfetchID AF050216; SV 1; linear; genomic DNA; STD; PRO; 3496 BP. XX AC AF050216; XX DT 16-MAR-1998 (Rel. 55, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus subtilis BglI modification methyltransferase (bglIM) and BglI DE restriction endonuclease (bglIR) genes, complete cds. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3496 RA Lunnen K.D., Wilson G.G.; RT "Method for producing the BglI restriction endonuclease and methylase"; RL Unpublished. XX RN [2] RP 1-3496 RX DOI; 10.1093/emboj/17.18.5466 RX PUBMED; 9736624. RA Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., RA Philips S.E.V.; RT "Crystal structure of restriction endonuclease BglI bound to its RT interrupted DNA recognition sequence"; RL EMBO J. 17(18):5466-5476(1998). XX RN [3] RP 1-3496 RA Lunnen K.D., Wilson G.G.; RT ; RL Submitted (20-FEB-1998) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs, 32 Tozer Rd, Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..3496 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /note="synonym: Bacillus globigii" FT /db_xref="taxon:1423" FT CDS complement(264..1310) FT /codon_start=1 FT /transl_table=11 FT /gene="bglIM" FT /product="BglI modification methyltransferase" FT /function="recognizes ds DNA sequence GCCNNNNNGGC; FT methylates one cytosine within this sequence at N-4 FT position; methylates both strands of the duplex, one strand FT at a time" FT /note="M.BglI; member of beta family of N-4 cytosine FT DNA-methyltransferases" FT /db_xref="GOA:O68556" FT /db_xref="InterPro:IPR001091" FT /db_xref="InterPro:IPR002941" FT /db_xref="UniProtKB/Swiss-Prot:O68556" FT /protein_id="AAC63974.1" FT /translation="MNNHSYLKKNSFHEGDARELLKCIEEESIALSVWSPPYHVGKKYE FT EGQTYEQWSSLLTKVIALHYPILKPGGFLVINIDDILAFPDPRMPRFQAVNLKKHRVSV FT TREDILNALKLEPELTKYQLAKKFNCSEQTIERRLKGNNIRGGKYNVQTKVKLAGPVLE FT KAAEEAGLYLYDRRIWAKDPAWQNSQWHSNSYKAVSEFEHLYIFWKPGETVIDRNKLSK FT EEWASWASRGIWYIPSVRKNDDHEAKFPLLLPQRLIKLLTQKGDTVLDCFMGSGTTAVA FT ALSESRNFIGIEKEPKYIQLSNKNVETFYISRNKEASKIKETNHSVTDEKKKAEQLELL FT LEENENSL" FT RBS complement(1317..1321) FT /gene="bglIM" FT RBS 1444..1449 FT /gene="bglIR" FT CDS 1457..2356 FT /codon_start=1 FT /transl_table=11 FT /gene="bglIR" FT /product="BglI restriction endonuclease" FT /function="recognizes ds DNA sequence GCCNNNNNGGC; cleaves FT both strands of the duplex thus: GCCNNNN/NGGC" FT /note="R.BglI" FT /db_xref="GOA:O68557" FT /db_xref="InterPro:IPR011335" FT /db_xref="InterPro:IPR011543" FT /db_xref="PDB:1DMU" FT /db_xref="UniProtKB/Swiss-Prot:O68557" FT /protein_id="AAC63973.1" FT /translation="MYNLHREKIFMSYNQNKQYLEDNPEIQEKIELYGLNLLNEVISDN FT EEEIRADYNEANFLHPFWMNYPPLDRGKMPKGDQIPWIEVGEKAVGSKLTRLVSQREDI FT TVREIGLPTGPDERYLLTSPTIYSLTNGFTDSIMMFVDIKSVGPRDSDYDLVLSPNQVS FT GNGDWAQLEGGIQNNQQTIQGPRSSQIFLPTIPPLYILSDGTIAPVVHLFIKPIYAMRS FT LTKGDTGQSLYKIKLASVPNGLGLFCNPGYAFDSAYKFLFRPGKDDRTKSLLQKRVRVD FT LRVLDKIGPRVMTIDMDK" XX SQ Sequence 3496 BP; 984 A; 663 C; 624 G; 1225 T; 0 other; gaattctttt agcaattcat acgcatcctg gggtgaccgt atggttcttt tcttgtaaag 60 tatgctagac tctttaacta atttaactga actgaaacaa tattgactct ttttgccgag 120 gtattttcca ttaaaattca ctcctaatag aaaattaaaa agaggaagtt cacacgtcta 180 cttaacgtgt gaacttcctc ttttatatcc actgttataa tatatatttg tattttttga 240 agatacggga taatgttata gacttaaaga gaattttcat tctcttctaa tagcaactcc 300 aattgttcag ccttcttttt ttcatcagta acactgtgat tggtttcttt aattttagaa 360 gcttctttat tcctacttat gtagaaagtc tccacatttt tattagaaag ttgaatgtat 420 ttcggttctt tttctatacc tataaaattc cttgattcac ttaaagcagc tacagcagta 480 gttccgcttc ccatgaaaca atctaatacc gtatctcctt tttgagttag aagttttatc 540 aacctttgag gtagtaaaag agggaactta gcctcatggt cgtcgttttt cctcacagac 600 gggatatacc atattcctct actagcccaa cttgcccatt cttctttcga tagcttattt 660 ctatcaataa cagtctctcc aggtttccaa aatatatata aatgctcaaa ttctgaaacg 720 gctttataag aattagaatg ccattgcgaa ttttgccaag cagggtcttt agcccatatt 780 cttctatcat ataaatacaa gccggcttct tcagcagctt tttctaaaac agggcctgct 840 aatttcacct tagtttgcac attatatttt ccgcctcgaa tattattacc ctttaatctt 900 ctctcaattg tttgttcact gcaattaaac ttctttgcca attggtattt tgttaactca 960 ggttccaatt ttaaagcatt tagtatatct tctcttgtta cactgactct atgtttcttc 1020 aggttaactg cttggaacct tggcatcctt gggtcaggga atgctaatat atcatcgatg 1080 ttaattacta aaaaaccacc tggctttaga atgggataat gcaacgctat gacttttgtt 1140 aaaagtgatg accattgctc gtatgtttga ccttcttcat attttttacc tacatgataa 1200 gggggagacc atacacttaa tgcaatactc tcttcttcaa tgcattttaa taattctcta 1260 gcatctcctt catgaaagga gttcttcttt aagtaactat ggttattcat acatttcctc 1320 caaaataaaa actctaaata tcattatatc aattcctcga ataccattca cttaacacat 1380 tgtaccttta tttacattta gtaattaatc tttgtttctc aatgtggtat aaattagtat 1440 aagaggtgaa atgagaatgt ataatttaca cagagaaaaa atcttcatgt cctataatca 1500 aaataagcaa tacttagagg acaaccctga gattcaagaa aaaattgagc tgtatggctt 1560 aaacttatta aatgaagtaa ttagtgataa cgaagaagaa atacgcgctg actataacga 1620 agctaacttt ctacatccat tttggatgaa ttatccaccg ttagaccgag gaaaaatgcc 1680 caaaggtgac cagataccat ggatagaagt tggcgaaaaa gctgttgggt ctaagctaac 1740 aagacttgtt tctcaaagag aagatataac agttagagag ataggtcttc ctacaggacc 1800 tgatgaaaga tacttgttaa cttctcctac tatttatagc cttacaaatg gatttactga 1860 ttcaataatg atgtttgttg atattaaatc agttggccct agagacagtg attacgattt 1920 agtattgtcc cctaaccaag tttcaggaaa cggtgattgg gcacagttag aaggtggtat 1980 ccaaaataat cagcaaacaa tccaaggacc tcgttcctca caaatatttc ttcctactat 2040 accaccgtta tatattctaa gtgatggtac aattgctcca gttgtgcatc tttttatcaa 2100 gccgatttac gccatgcgct cgctaactaa gggagatacg ggacaatctc tttataaaat 2160 taaattagca tctgttccta atggtttggg tttgttttgc aacccaggtt acgcatttga 2220 cagtgcttat aaatttttat tcagaccagg taaagatgat aggactaaat cacttttgca 2280 aaaaagggtt cgggttgact taagagtact ggataagatt ggacctagag ttatgacaat 2340 tgatatggat aagtaaaatg taactacaga gaagcagcct ttctttataa cgattggctg 2400 ctttatttca taaaaacctc atttacttat ggtacggttc ccacgattaa tccgaaaagc 2460 cgataaatct gctcaactag tatgagtcgc atcaactgat gaggaaacgt catcttcgag 2520 aacgaacagt ttatcatccg ctcgcttcat caccgcgtca ctcaatccga gtgatccgcc 2580 gatgacgaag gtgactttgc tttttccata agtagccagc ttatctattg tatcggctag 2640 ttcttcggat gttttcattt ttccttcgat ggcgagggcg atgacgtggg cgtcggggct 2700 gatttttgat aggatgcggt cgccttcttt gtcttttatg attttcatgt cctggtcgct 2760 tagattttcc ggcgcttttt cgtccggtag ttcgatgatg tcgatttttg cgtaggccga 2820 aagtcgtttt gtgtattctt caattccttg cttgaggtat ttttctttta gttttccgat 2880 tgtcacaata ttgatattca caggtcatcc ccaccttaga aacaagttat tcatatatgt 2940 tgtccacatt tgtggatatc ttttctgtat tttgtgtaag atcattcgtt ccccactata 3000 tatactgcgg gctcactaca taattcacag gctgtggata aactgttagt atgttcgatt 3060 tttctgattt cgggcgggag ttcgtggtcg tctatgtaca tatccagcac ggtttcgatg 3120 tgttcttcac aggaataata agcatttttc atttttagtc ctccgatgtt ttcttgcatt 3180 ctgcaaatgt tctcttccca atataaccta tttattcaca tgttagtagt gcttcgggct 3240 gagttttatc cacaattcga caagcgaagc tgttgatgac tctatttcgt ccgtatgtta 3300 gttttgatgt aaccgaagtt tggggaggtc tgcatttgaa actcagatgg gtgtggcttt 3360 tcgtgatcat gttgttgctt gctgcgtgtc acccagtctt attgggtgtt agagaaggat 3420 cggatcggtg aaggcagttc cgagctagaa ttatatgcaa attatcttca aacacatgaa 3480 aacgtaatag ggtacc 3496 // |
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