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EBI DbfetchID AF044847; SV 1; linear; genomic DNA; STD; PRO; 4062 BP. XX AC AF044847; XX DT 16-FEB-1998 (Rel. 54, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Acetobacter pasteurianus putative DNA invertase (rin), ApaLI DE methyltransferase (apaLIM), and ApaLI restriction endonuclease (apaLIR) DE genes, complete cds. XX KW . XX OS Acetobacter pasteurianus OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; OC Acetobacteraceae; Acetobacter. XX RN [1] RP 1-4062 RX DOI; 10.1007/s004380050890 RX PUBMED; 9862476. RA Xu S.Y., Xiao J.P., Ettwiller L., Holden M., Aliotta J., Poh C.L., RA Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., RA Slatko B., Benner J.; RT "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI RT restriction-modification systems in Escherichia coli"; RL Mol. Gen. Genet. 260(2-3):226-231(1998). XX RN [2] RP 1-4062 RA Xu S.-Y., Dalton M.A.; RT ; RL Submitted (27-JAN-1998) to the EMBL/GenBank/DDBJ databases. RL Research, New England Biolabs, Inc., 32 Tozer Road, Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..4062 FT /organism="Acetobacter pasteurianus" FT /mol_type="genomic DNA" FT /db_xref="taxon:438" FT repeat_region 806..854 FT /rpt_type=INVERTED FT CDS 930..1511 FT /codon_start=1 FT /transl_table=11 FT /gene="rin" FT /product="putative DNA invertase" FT /db_xref="GOA:O30699" FT /db_xref="HSSP:2RSL" FT /db_xref="InterPro:IPR006120" FT /db_xref="UniProtKB/TrEMBL:O30699" FT /protein_id="AAC97179.1" FT /translation="MNQRIGYARVSTQDQHFDLQRDALLQAGCGVIYEDTASGKNAMRP FT ELEQCLKALRSGDTLVVWRLDRLGHSLPDLVQVVSDLERSGIGFESLTEKIETVSAAGK FT LVFHVFAALAEFERSLIRERTHAGLAAVRARGRSGGRKPKLGEKQVREIKALLRDPDIR FT VADVARRYGVSRTTLYKHVGVIAPRQWDMA" FT CDS 1508..2797 FT /codon_start=1 FT /transl_table=11 FT /gene="apaLIM" FT /product="ApaLI methyltransferase" FT /note="C5 methyltransferase; modifies target sequence FT 5'GTGCAC3'" FT /db_xref="GOA:O52702" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:O52702" FT /protein_id="AAC97180.1" FT /translation="MNKDEVVVSLFAGAGGFSSGFSQAGLKPLFGAEINADACQTYQEN FT VGSPCHQLDLSTVDPSHIEMLTGGKRPFVVIGGPPCQGFSTAGPRNFADPRNLLIFNYL FT NIVERLSPRWLIFENVEGLLTSGGGRDLARLVREFVDMGYSVRLQKVNLAAYGVPQTRK FT RVLIIGNRLGIDFQFPEELYSFDSGKAKKASGKPLAPSLAEAVAGLGPAASDKDALVPY FT ASSEPVNAFDARMRAGNRVEVVTHHVRVEAAERMQVELLKPGQTMKDLPPELWHESYRR FT RANRRVSDGTPTEKRGGAPSGIKRLHGNLQSLTITGPAAREFIHPTEHRPLTIRECARI FT QTFPDKYRWVGNNASVIQQIGNAVPPLAAERLAKHLRDIDGSFGADTRPAGAMSAKLLG FT FVLTEALGMSPALKSTEALLAEMHQGGFVF" FT CDS 2797..3924 FT /codon_start=1 FT /transl_table=11 FT /gene="apaLIR" FT /product="ApaLI restriction endonuclease" FT /note="recognition sequence 5'G|TGCAC3'" FT /db_xref="GOA:O52703" FT /db_xref="InterPro:IPR019036" FT /db_xref="UniProtKB/Swiss-Prot:O52703" FT /protein_id="AAC97181.1" FT /translation="MTTRQRLSAERSQQLTRLLTITKTANMRALMEASELAKVIALVAV FT DIGKSDEMARAFPVLWPKISPQQEYYATAVDWFTNPDETVTSFDVVDMLDAGTSLDQDF FT MTYLKCLTELHKRRRKYGLILQRQPLPTMVQVSPRALMEYGPDFPPEALASWLTWRKFF FT YDLDNRSAQETGYLFEPILAAAIGGEAKSARERVVRRTDDPTKGRQVDCWKVLPDGTPL FT AYELKLRVTIAASGQGRFGEELSFARDCSSSGAKPILVVLDPTENDKLTGLQAAYREVG FT GAAYVGDAAWAHLEDEAGATMASFIERYVRVPVASVSSFERVIEGDATKRSLILQDLQA FT RLDGNELTISLGGHQRLVERHEDQSLAADGDDDSE" XX SQ Sequence 4062 BP; 870 A; 1044 C; 1202 G; 943 T; 3 other; gatatctggg tgtcgatgtt gaggatgccc tcgccttatc agaggcgaca gacctgtgac 60 gtctcctgcg tttgtcactt gctctctccc tatgtgtaca tgatatgatg acacataggg 120 aggatcacat gagtgaacag ctttctggca gccagagcgc gccactgaaa atcggggtgc 180 gggagttccg tggcaacctg accgcttacc ttcgccaggc gcggcaaggc agtacgatcc 240 tggtcacatc ccatgatgag atcgtggccg aactgcgccc gccatccccg tcctatcgcc 300 cccgtcggaa gccgggtggg ctacgcgggc gcatcaagct aagtgatgat tttgatgaga 360 cgccgcagga catgctcgac agcatggacg gggccctgtg aaggttctgc tcgatacaca 420 tgcgctgcta tggtggttgt ccgactctga caaactggga cctgcagcac gtgagatcgt 480 tgcggatccg gcccacgaca ttctggttag tattgtctcg ctctgggaga tcgcgatcaa 540 aatccggatc gggaagctgg atgcagatct caacgagatc acggatacta ttccggcaga 600 gggttttacg cttctgccga tttcccccga gcacctgaag atcctgatcg gggstgccta 660 tccatcaccg tgatcccttt gatcacctgc ttatggcgca ggcactgagt gaacaggcgt 720 catttctgtc cggagatagc cagatggacc attacccaat cgagaggatc tcttgctcat 780 aactgtgcgg gtaattgctt tataagtttt tcgccttgtc cagaaaacgg tggttttctg 840 gacgctgtaa aaacggcctt tccgggctag tcggatgcct cccgtcgagt taatattgcc 900 gtcaagtttt caatgtcccg gtttggtcaa tgaatcagcg tatcggatat gcacgggtct 960 ccacgcagga ccaacacttt gatctgcagc gcgatgcgct cctgcaagct ggctgcgggg 1020 tcatctacga ggataccgca agcggtaaga acgccatgcg gccggagctg gagcaatgcc 1080 tcaaggcctt acgcagcggg gatacgctgg tcgtgtggcg cttggatagg cttggtcaca 1140 gcttgcccga cttggtgcag gttgtaagtg atcttgagcg cagcggcatt ggctttgaaa 1200 gcctcactga aaaaatcgag acggtcagcg cggctgggaa gttggtcttc cacgtctttg 1260 cagccctcgc ggaattcgag cgcagtctga tccgcgagcg cacccacgcg gggcttgcag 1320 cagtgcgtgc cagagggcgc tccggtggac gcaaacctaa actcggtgag aagcaggtgc 1380 gagagatcaa ggcgcttttg cgtgatcctg acatccgagt tgccgatgtt gctcgtcggt 1440 atggcgtgtc tcgaaccact ctttacaagc acgttggtgt tatagcgccg cgtcaatggg 1500 atatggcatg aataaggacg aagttgttgt atctctgttt gctggggctg ggggattctc 1560 ctctggcttt agtcaggctg gtctgaagcc tttatttggt gcggaaatta acgctgatgc 1620 ctgccagact taccaagaga atgtcggcag cccctgccac cagcttgatc tgagcacggt 1680 tgacccatct catattgaaa tgctcacggg tggcaaaagg ccgttcgtcg ttattggcgg 1740 gccgccgtgc caaggcttta gtaccgctgg tccgcgcaat tttgcagatc ctcgcaatct 1800 gctcattttt aactacttaa atatcgttga gcggctttcg ccccgttggc tcattttcga 1860 aaatgtcgaa ggacttttga cgtctggtgg ggggcgggac cttgcgcgtc tggtacgaga 1920 gtttgtcgac atgggatatt cggtacggct ccaaaaggta aacctagccg catatggcgt 1980 gccacaaacg cgtaagcggg tgctaatcat tggcaaccga cttgggatcg attttcaatt 2040 tcccgaggag ttgtattcat tcgatagcgg taaagcgaaa aaggcttccg gcaagccgct 2100 tgctccaagc ttggccgaag cggtggcagg gctcggtccc gctgcaagtg ataaggatgc 2160 gctagttccg tacgcgagct cggaacctgt caatgcattc gatgcccgaa tgcgggcggg 2220 aaatcgcgtc gaggtggtaa ctcaccacgt tcgagtcgag gctgcggagc gtatgcaggt 2280 tgaactgctc aaaccgggtc agacgatgaa agacctgccg cccgagcttt ggcatgagag 2340 ctacaggcgg cgagcaaacc ggcgagtatc ggacggtacg ccaaccgaga agcggggcgg 2400 cgcgccttca gggatcaaac gtctgcatgg caatctgcag agcctcacga tcacggggcc 2460 tgctgctcgg gaatttattc atcctacaga gcatcgtcct ttgaccattc gcgagtgtgc 2520 ccgcattcag acatttcccg acaagtacag gtgggttggc aacaatgcca gcgtcattca 2580 gcaaattgga aacgctgtgc caccgctcgc tgctgaacgt ttggccaaac acttaagaga 2640 tattgatggg tcgtttggtg cagatacgcg accggcaggt gcgatgtctg caaagctatt 2700 aggatttgtt cttactgaag cgcttggaat gagtccggca ctcaaatcaa cagaagcgct 2760 gcttgcagaa atgcaccaag ggggattcgt tttttaatga ctacacggca acgactcagc 2820 gcagagcgat cgcagcagct cactcgcctg cttacgatca cgaagacagc aaacatgcgg 2880 gcgctcatgg aggccagcga gctagccaaa gtaattgcac ttgtggccgt tgacatcggc 2940 aagagtgatg aaatggcgcg agcatttcct gtgctctggc cgaaaatttc tccgcaacag 3000 gagtactacg ccacggcagt tgactggttc accaaccccg acgagacggt tacgtccttt 3060 gatgttgtcg atatgcttga tgcaggcacg tcactggatc aggacttcat gacctatctg 3120 aaatgcctca ccgagcttca taagcgccgt cgtaaatatg gattgattct tcagaggcag 3180 cctctgccga ctatggtcca agtctcccct agggcgctca tggaatacgg ccctgacttt 3240 ccgccggaag cacttgcttc atggctcact tggcgcaaat tcttttacga tttggacaat 3300 cggtctgcac aggaaaccgg ctatcttttc gaacccatcc ttgctgcagc catcggcggt 3360 gaggcgaagt cggcgcggga gcgtgtggtg aggcgcactg atgaccctac taagggtcgg 3420 caagtcgact gctggaaagt gctgccggac ggaactccac ttgcgtacga attgaagttg 3480 cgcgtgacga ttgcggctag cgggcaaggg cgttttggcg aggagctctc atttgctcgc 3540 gactgcagta gttcgggtgc aaagccaatt cttgttgtgc tggatcccac agaaaatgac 3600 aaactgaccg ggcttcaggc cgcgtaccga gaggtgggcg gcgccgctta tgtgggcgat 3660 gcggcttggg ctcacctcga agacgaggcg ggggcaacga tggcatcgtt tattgaacgc 3720 tatgttcgcg tgccggtggc ttctgtgtcc agctttgagc gggttatcga gggtgacgca 3780 accaagcgta gtctcatctt gcaggacctg caggcacggc ttgatggcaa tgaactgaca 3840 atctcgcttg gtggccatca acgcttggtc gagcgtcatg aagatcagag tttggctgcc 3900 gatggggatg atgacagcga ataggttgaa ctatgcggta aggggggcga aggtctgtcc 3960 cgcgagcaga tagagtgcca gaacggtatt cagcattgnc agccatactg acgatcctcc 4020 nctcccgctt tgatggtcga tggtatttct catggcattg at 4062 // |
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