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EBI DbfetchID AF039750; SV 1; linear; genomic DNA; STD; PRO; 2326 BP. XX AC AF039750; XX DT 13-JAN-1998 (Rel. 54, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Streptomyces fimbriatus SfiI endonuclease (sfiIR) and SfiI DE methyltransferase (sfiIM) genes, complete cds. XX KW . XX OS Streptomyces fimbriatus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RP 1-2326 RA VanCott E.M., Moran L.S., Slatko B.E., Wilson G.G.; RT "Characterization of the SfiI restriction and modification genes"; RL Unpublished. XX RN [2] RP 1-2326 RA VanCott E.M., Moran L.S., Slatko B.E., Wilson G.G.; RT ; RL Submitted (22-DEC-1997) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..2326 FT /organism="Streptomyces fimbriatus" FT /mol_type="genomic DNA" FT /db_xref="taxon:68197" FT CDS 94..903 FT /codon_start=1 FT /transl_table=11 FT /gene="sfiIR" FT /product="SfiI endonuclease" FT /function="cleaves ds DNA GGCCNNNNNGGCC to yield FT GGCCNNNN/NGGCC" FT /note="R.SfiI; type II restriction enzyme" FT /db_xref="GOA:O52512" FT /db_xref="PDB:2EZV" FT /db_xref="UniProtKB/Swiss-Prot:O52512" FT /protein_id="AAB95365.1" FT /translation="MHQDYRELSLDELESVEKQTLRTIVQALQQYSKEAKSIFETTAAD FT SSGEVIVLAEDITQYALEVAETYPINRRFAGFIDYKRVRWLPSPHGLLPQVLLVDAKAS FT TEKNRDTLQRSQLPMDAEFRNTSSGEVVTMEAGVIPHLMLQSANDGVLPAVTTSIFVHF FT YYRELKDVEGRYRELKSIYVLSLPHARLKQRYNPDPDTSFFGAGKHSPARGEVARIRVY FT FDRLKEACPWRLQELHYSADSEYTQPRWRDLNDAGHEVTKEFLFLER" FT CDS complement(872..2137) FT /codon_start=1 FT /transl_table=11 FT /gene="sfiIM" FT /product="SfiI methyltransferase" FT /function="methylates one cystosine on each strand within FT the DNA sequence GGCCNNNNNGGCC" FT /note="M.SfiI; beta class N-4 Cyt DNA MTase" FT /db_xref="GOA:O52513" FT /db_xref="HSSP:1G60" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:O52513" FT /protein_id="AAB95366.1" FT /translation="MRKPSSGQPAGLDAWDETRSLATHAGPDFALYVGDSLDCLAKLPD FT ESINTVVTSPPYWAVRDYEHDEQLGLEDEVDDYVERLVKIFREVYRVLATDGSAWLNIG FT DSYFNKQITVGGKPPRTGWKRNKQLSLVPFRVALALQDDGWWIRNVAVWHKPNAMPASV FT RDRLTVTWEPVFLLTKSERYYFNLDEIRVPHQTSDAIERRRAESGTVTGKAQGKKELRK FT WLNSPRHRATIEGIKEVERRPNAPAAVELASYLRTALKEKKRSIAWVAEQLDLPFERTR FT HYFRTDEIGSRLPPPEVWEQLKDLLELDATYDEAMTVEVGDNVFRNHPNGKNPGDLLSI FT PTAPSGANHFAVMPRKLAHFALKATLPMNGSCLDPFMGSGTTGRVVRELGGRFVGVDVN FT EHYMTDYLVESGVISPETETLW" XX SQ Sequence 2326 BP; 494 A; 628 C; 675 G; 529 T; 0 other; ggttcagaag gtaacaagcg tcataagcta ggtccccact tagcgatcat cgggcggtgt 60 catggacatc gctcctggct agtggggggc cacatgcacc aggactacag ggaactttct 120 ctcgacgagc tcgaaagtgt agagaagcag acgctacgaa ccatcgttca agccttgcag 180 cagtattcaa aggaggcaaa gtccatcttt gaaactactg cagctgactc tagtggcgaa 240 gttatcgtgc ttgccgagga tataactcag tacgcgctgg aggttgcgga gacctaccca 300 atcaatagga ggtttgccgg cttcatcgat tataagaggg ttcgctggct tccctctccg 360 catgggctcc tcccgcaggt gcttctagta gacgctaaag cgtctacgga gaagaaccgt 420 gacactcttc agcgatctca acttccaatg gatgcggaat ttcgcaatac cagttccggt 480 gaagtggtca ccatggaagc cggggtcatc ccgcacctca tgttgcaatc ggcgaatgat 540 ggagttcttc ccgccgtaac cacgtccatt tttgttcact tttactacag ggagttgaag 600 gacgtagaag ggcgttaccg cgagttgaag tcgatctatg tcctaagcct cccgcatgcg 660 cggttgaagc agcgctacaa ccccgatccg gatacttcat tcttcggagc ggggaagcat 720 tcgccggcgc gaggtgaggt ggcgcgaatc cgcgtttact ttgaccgcct taaggaggct 780 tgcccttggc gtttgcaaga acttcactat tccgctgata gtgagtacac gcagccccgt 840 tggcgtgatt tgaatgacgc cgggcatgaa gtcaccaaag agtttctgtt tctggagaga 900 tgactccaga ttcaactagg tagtccgtca tgtagtgctc gttcacatct actccgacga 960 aacgcccgcc gagttcgcgc accactctgc cagtggtacc acttcccatg aatgggtcaa 1020 ggcatgaacc gttcatggga agtgtggctt tcagtgcgaa gtgggcaagc tttctgggca 1080 taacggcaaa atggtttgcg ccactgggag ctgtcgggat gctcagtagg tcgccggggt 1140 tcttcccgtt ggggtggttc cggaagacgt tgtctcctac ttccacggtc atggcttcgt 1200 catacgtagc gtcgagctct agaagatcct tcagttgctc ccatacttca ggcggaggta 1260 gacgcgagcc aatttcatcc gtgcggaagt aatggcgggt acgctcgaaa ggaagatcga 1320 gttgctcggc cacccaagcg atggagcgtt tcttttcctt cagtgctgtg cgcaggtagg 1380 atgcaagttc tacagcggcc ggcgcattgg ggcggcgttc gacttccttt attccttcga 1440 tggtggcgcg atggcgcgga gagttaagcc acttgcgtag ctctttcttt ccctgtgcct 1500 ttccggttac agttcctgat tccgcgcggc gacgttcaat ggcgtcactg gtctgatgcg 1560 ggacgcgaat ctcgtccagg ttgaagtagt aacgctcaga cttagtgagg aggaaaactg 1620 gttcccaggt aactgtcaga cgatcgcgaa cgctggcagg catcgcattc ggcttgtgcc 1680 aaactgcaac gttcctaatc caccatccgt catcctgcaa ggctagagca acccgaaaag 1740 gcacaaggga aagctgctta ttacgcttcc agcctgtgcg cggtggtttc ccgccgaccg 1800 tgatctgctt gttgaagtaa gaatcgccaa tatttagcca tgcgctaccg tcagtggcaa 1860 gtacgcggta gacctcgcgg aagatcttca ccagacgctc tacatagtcg tctacctcgt 1920 cttccaagcc gagctgctcg tcgtgttcgt agtcacgcac agcccagtac gggggcgagg 1980 taaccaccgt gttgatcgac tcgtccggaa gcttcgccag gcagtcgagg gagtcaccga 2040 cgtacagagc gaagtcaggg ccggcgtgtg tagcgaggga tcgtgtctcg tcccaagcat 2100 cgaggcctgc tggctgaccg gaagagggtt tgcgcatggt tcgtaagcgt acctactggc 2160 actgacgggg acagcaccct tgcctgccag ggggcctggc agccggcggt gtacagcagc 2220 gatgcacagc aaccagcgcg tccaggggtg cccgagcatg gtcctcagca gctcaggtgt 2280 gacctgtacg accggcaacg accgatccgc atatagctac ggatca 2326 // |
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