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EBI DbfetchID AF038547; SV 2; linear; genomic DNA; STD; PRO; 7686 BP. XX AC AF038547; XX DT 05-NOV-1998 (Rel. 57, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus stearothermophilus beta-1,4-mannanase (manF), esterase (estA), DE transcription regulator (repA), and alpha-galactosidase (galA) genes, DE complete cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-2645 RX PUBMED; 9797302. RA Ethier N., Talbot G., Sygusch J.; RT "Gene cloning, DNA sequencing, and expression of thermostable RT beta-mannanase from Bacillus stearothermophilus"; RL Appl. Environ. Microbiol. 64(11):4428-4432(1998). XX RN [2] RP 2296-7686 RA Ethier N., Talbot G., Sygusch J.; RT "Cloning, sequencing and expression of a Bacillus stearothermophilus RT alpha-galactosidase gene clustered with a beta-mannanase gene"; RL Unpublished. XX RN [3] RP 1-2645 RA Ethier N., Talbot G., Sygusch J.; RT ; RL Submitted (13-DEC-1997) to the EMBL/GenBank/DDBJ databases. RL Biochimie, Universite de Montreal, C.P.6128, Succursale Centre-ville, RL Montreal, Quebec, Canada XX RN [4] RC Sequence update by submitter RP 1-7686 RA Ethier N., Talbot G., Sygusch J.; RT ; RL Submitted (16-APR-1999) to the EMBL/GenBank/DDBJ databases. RL Biochemistry, University of Montreal, C.P.6128, succursale Centre-Ville, RL Montreal, Quebec H3C 3J7, Canada XX CC On May 15, 2000 this sequence version replaced gi:3834387. XX FH Key Location/Qualifiers FH FT source 1..7686 FT /organism="Geobacillus stearothermophilus" FT /strain="MCA2184" FT /mol_type="genomic DNA" FT /db_xref="taxon:1422" FT CDS complement(211..2295) FT /codon_start=1 FT /transl_table=11 FT /gene="manF" FT /product="beta-1,4-mannanase" FT /note="glycosyl hydrolase family 5" FT /db_xref="GOA:Q9ZI87" FT /db_xref="HSSP:1OF4" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9ZI87" FT /protein_id="AAC71692.1" FT /translation="MNKKWSYTFIALLVSIVCAVVPIFFSQNNVHAKTKREPATPTKDN FT EFVYRKGDKLMIGNKEFRFVGTNNYYLHYKSNQMIDDVIESAKKMGIKVIRLWGFFDGM FT TSENQAHNTYMQYEMGKYMGEGPIPKELEGAQNGFERLDYTIYKAKQEGIRLVIVLTNN FT WNNFGGMMQYVNWIGETNHDLFYTDERIKTAYKNYVHYLINRKNQYTGIIYKNEPTIMA FT WELANEPRNDSDPTGDTLVRWADEMSTYIKSIDPHHLVAVGDEGFFRRSSGGFNGEGSY FT MYTGYNGVDWDRLIALKNIDYGTFHLYPEHWGISPENVEKWGEQYILDHLAAGKKAKKP FT VVLEEYGISATGVQNREMIYDTWNRTMFEHGGTGAMFWLLTGIDDNPESADENGYYPDY FT DGFRIVNDHSSVTNLLKTYAKLFNGDRHVEKEPKVYFAFPAKPQDVRGTYRVKVKVASD FT QHKVQKVQLQLSSHDEAYTMKYNASFDYYEFDWDTTKEIEDSTVTLKATATLTNKQTIA FT SDEVTVNIQNASAYEIIKQFSFDSDMNNVYADGTWQANFGIPAISTPKTRCLRVNVDLP FT GNADWEEVKVKISPISELSETSRISFDLLLPRVDVNGALRPYIALNPGWIKIGVDQYHV FT NVNDLTTVTIHNQQYKLLHVNVEFNAMPNVNELFLNIVGNKLAYKGPIYIDNVTLFKKI FT " FT CDS 2628..3617 FT /codon_start=1 FT /transl_table=11 FT /gene="estA" FT /product="esterase" FT /db_xref="HSSP:1L7A" FT /db_xref="InterPro:IPR008391" FT /db_xref="UniProtKB/TrEMBL:Q9JP86" FT /protein_id="AAF70202.1" FT /translation="MFDMPLAQLQKYMGTNPKPADFADFWSRALEELSAQSLHYELIPA FT TFQTTVASCYHLYFTGVGGARVHCQLVKPREQKQKGPGLVWFHGYHTNSGDWVDKLAYA FT AAGFTVLAMDCRGQGGKSEDNLQVKGPTLKGHIIRGIEDPNPHHLYYRNVFLDTVQAVR FT ILCSMDHIDRERIGVYGASQGGALALACAALEPSVVKKAVVLYPFLSDYKRAQELDMKN FT TAYEEIHYYFRFLDPTHEREEEVFYKLGYIDIQLLADRICADVLWAVALEDHICPPSTQ FT FAVYNKIKSKKDMVLFYEYGHEYLPTMGDRAYLFFCPIFFPIQKRNVK" FT CDS 3680..4408 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="transcription regulator" FT /note="similar to catabolite control protein" FT /db_xref="GOA:Q9LBD2" FT /db_xref="InterPro:IPR000843" FT /db_xref="UniProtKB/TrEMBL:Q9LBD2" FT /protein_id="AAF70203.1" FT /translation="MRDIARKLGVSSVTVSKALNDKEGVSEELRKKIKETAREMGYRLN FT TLTKAMKEGYSYNVGVVIPERFIGDTQSFYLRFYQHIAKALDHYGYCSILHILTAEDEE FT QLQLPRVYNERKVDGFIMLGQVNKEYASLFQQIELPVVFLDFYHDQDVDCVLTDNFFGM FT YELTNHLIQCGHREIAFVGNIYSTSSIQDRFLGYYKSLLEHRIPLREEYIISDRDEKGK FT YIDLYCRKTCRLLLYATVIK" FT CDS 4699..6930 FT /codon_start=1 FT /transl_table=11 FT /gene="galA" FT /product="alpha-galactosidase" FT /EC_number="3.2.1.22" FT /db_xref="GOA:Q9LBD1" FT /db_xref="InterPro:IPR002252" FT /db_xref="UniProtKB/TrEMBL:Q9LBD1" FT /protein_id="AAF70204.1" FT /translation="MPIQVNEETKEFHLFNENISYIFRVLEKSNQLEHLYYGKRIKHRD FT SFQHLIEREVRPSCNMFEGDYTSSLEHIKQEYPSYGTTDYRYPSYVIMNDIGSTVTNFQ FT YDSYRIIAGKPKLPFLPATYVENPEEADTLEVTLIDDVLQCKLVLSYTIYRHRNVICRN FT AKLVNEGEKAFYIHHAMSSSVDFPDSDFEMVQLSGAWAREFHLETKKITKGIQSVYSVR FT GASSHIHNPFLALKRPDATEHKGEVYGFSLVYSGNFLAQVEVDTYDVTRVLMGIHPFQF FT RWKLYPTESFQTPECVMVYSDQGLNGMSQTYHDLYRTHLVRGPWRDRPRPILINNWEAT FT YFDFNEEKILQIAKTAKELGIELFVLDDGWFGNRNDDTSSLGDWFENREKLPSGIKGLS FT EKIEALGMKFGLWFEPEMVCKDTKLFQEHPDWVIATPNRRMSHGRNQFVLDFSKQEVVD FT YIFGLMSHVIENAKISYIKWDMNRYITEPYSNGLPADRQGEVFHRYILGVYQLYERLLE FT AFPHILFESCAGGGGRFDPGMLYYAPQAWTSDDTDAVERLKIQYGASLVYPLSSIGSHV FT SAVPNHQVGRITPLDTRANVAYFGTFGYELDITKLTDEEKEKVKQQVTFFKEKRQLIHH FT GRFYRLLDPFHSNEVAWMVVSEDQKEAIVGYYQVLAKPNDRYHRLKLKGLHPDFLYVVE FT GKGTTHYGDELMNVGMIFAENYTDRAHEYWQREKKGDFASQVFVLKAIS" XX SQ Sequence 7686 BP; 2233 A; 1368 C; 1800 G; 2285 T; 0 other; tgatgatcat gacctagaga catgctgatg tcggcgatgg ttgacggcga cgacgatatt 60 atataagcat cacagagggt gtttccaaaa gcaaaatgat ggacaccctt ttttctgcgt 120 ctcgatcatc gtgaaggagc gttaaagaaa agacgaaaaa gatccgatgg cctttcatga 180 aaaggggcat cggatctttt acataaaatg ttatattttt ttaaatagcg tgacgttgtc 240 gatataaata ggacccttat aagctaattt atttccgacg atgttaagaa acagctcgtt 300 aacattaggc atagcattaa attcaacatt aacgtgtagc aatttgtatt gttggttatg 360 gattgtaaca gtggttaagt catttacatt gacatggtat tggtcgacac cgattttgat 420 ccaacctgga tttaaggcga tgtaaggacg taaagcgcca ttgacatcca cgcgaggcag 480 aagcaagtca aatgatatac gggatgtttc gcttaattca gagatagggc ttatttttac 540 ctttacttct tcccagtctg cattcccagg aagatctaca tttacacgca ggcatctcgt 600 tttcggcgtg ctaatggcag gaattccaaa gttggcttgc catgtgccat ctgcatatac 660 gttattcata tccgaatcaa aagaaaattg tttgatgatt tcatatgcgg aagcattttg 720 gatattgacc gtcacttcat cggatgcaat cgtttgtttg ttcgttaaag tggcagttgc 780 ttttaaggta actgtactgt cctctatttc ttttgtggta tcccagtcaa attcgtaata 840 atcgaatgaa gcattatatt tcatcgtata agcttcatca tggctgctaa gttgcagctg 900 tactttttgc actttgtgct ggtcactcgc cacttttact tttacacgat aagtgccgcg 960 aacatcttgt ggttttgcag ggaaggcaaa atatactttt ggttcttttt cgacatgtct 1020 atctccgtta aacaatttag catatgtttt tagcaggtta gtaaccgagc tatgatcatt 1080 gacaatccgg aatccgtcat aatcaggata gtaaccattt tcatccgcgc tttcagggtt 1140 gtcatcgatg ccagtcagaa gccaaaacat tgcaccggtg ccgccgtgtt cgaacatcgt 1200 ccgattccat gtatcatata tcatttcacg gttttgcact cctgtggcgc taattccgta 1260 ttcttcgagc acaactggtt ttttcgcttt ctttccggca gcaagatggt caagaatata 1320 ttgttctccc cacttttcga cgttttccgg cgaaattccc caatgttcag gatataaatg 1380 gaatgtcccg tagtctatat tttttaaagc aattaaccgg tcccagtcca ccccgttata 1440 accagtgtac atataggaac cttcaccatt gaatccaccc gacgaacggc ggaagaatcc 1500 ttcgtctcct actgcaacaa ggtgatgagg gtctattgat ttaatatatg tgctcatttc 1560 atcggcccag cgaacaagcg tatcccctgt tgggtcagag tcgttgcgag gttcgttagc 1620 caattcccat gccataatcg tcggttcatt tttataaatg atgccggtat attggttttt 1680 ccgattgatt agataatgaa cgtaattttt gtaagcggtt ttaattcttt catctgtata 1740 gaataaatcg tgattcgttt ctccaatcca gttgacatat tgcatcatgc cgccgaagtt 1800 attccagttg tttgttaata cgatgactaa gcgaattcct tcctgcttgg ctttgtaaat 1860 ggtgtagtct aaacgttcaa aaccgttttg cgccccttct aattctttag gaatgggtcc 1920 ttctcccata tacttgccca tttcatattg catgtacgtg ttatgcgctt ggttttccga 1980 agtcatcccg tcaaaaaatc cccaaagacg aatcactttt attcccattt tttttgctga 2040 ttcaatgacg tcatcaatca tttgattgga tttgtaatga aggtaatagt tattggttcc 2100 gacaaagcga aactctttgt tgccaatcat taatttgtca cctttgcggt aaacaaattc 2160 attgtctttt gttggagtgg caggttccct ctttgttttt gcatgtacgt tgttttggga 2220 gaaaaagata ggtactacag cacaaacgat actaacaaga agcgcgatga aggtgtaact 2280 ccattttttg ttcatggatt aaactcctcc ttcttttatg aatttctaag tgaaagatct 2340 gggcagtcaa accatggtgt agtggagaat gaaaggtttg taggaagaca ggtaattaac 2400 tcgtggtgga tatagggaaa atatctggat gctgctaggt aaattttata aaacgttttc 2460 tatgttgtca acttaaattt aagtaatcgt taagtttaaa attataacta aatgaagtta 2520 atttagcagg aatataaaat aaaaatggcc gaatctataa acctatagat cgttgtattg 2580 tgttatttaa gtagagaatt taagtagaga aaggatgaac gttcaccatg ttcgatatgc 2640 cgttagcaca attacagaaa tacatgggga caaatccgaa gccggctgat tttgctgact 2700 tttggagtcg agcgttggag gaattatctg cccaatcgtt gcattatgag ctgattccgg 2760 caacatttca aacgacagtg gcgagttgct accatttgta tttcacggga gtcggcgggg 2820 ctagagtcca ttgtcagtta gtaaaaccga gagagcagaa gcagaaaggc ccggggttgg 2880 tatggtttca tggctaccat acgaatagcg gcgattgggt cgataaactg gcatatgctg 2940 cggcaggttt tactgtattg gcgatggatt gccgcggcca aggaggaaaa tcagaggata 3000 atttgcaagt gaaaggccca acattgaagg gccatattat tcgcggaatt gaggatccaa 3060 atcctcatca tctttattat cgaaatgttt ttttagatac agttcaggcg gtaagaattt 3120 tatgctctat ggatcatatt gatcgtgaac gaattggtgt atatggcgct tcccaaggag 3180 gagcgttggc attagcgtgt gctgctctgg aaccatcggt ggtgaaaaaa gcggttgtgc 3240 tctatccatt tttatcggat tataagcggg cgcaagagtt ggatatgaaa aataccgcgt 3300 atgaggaaat tcattattat tttcgatttt tagatcccac acatgagcgg gaagaagaag 3360 tattttacaa actaggctat attgatattc aactcttagc cgatcggatt tgtgccgatg 3420 ttttatgggc tgttgcgcta gaagaccata tttgtccccc gtccacacaa tttgctgttt 3480 ataataaaat taagtcaaaa aaagacatgg ttttgtttta cgagtatggt catgagtatt 3540 taccgactat gggagaccgt gcttatctgt ttttttgccc gatcttcttt ccaatccaaa 3600 agagaaacgt taagtaaatc ttaaaaaatt ctatcattct ttgaggacgg ggtcgaacat 3660 cgtgaaacaa cgtgtaacga tgcgtgatat agccagaaaa cttggagtga gcagcgtcac 3720 tgtttcaaaa gcactaaatg ataaggaagg tgtcagtgaa gagctgcgga aaaaaattaa 3780 ggaaacggcc cgggagatgg ggtatcgttt gaatacgctg accaaagcga tgaaggaagg 3840 gtattcgtat aacgttggtg tcgtcattcc ggaacgattt attggagata cacaatcatt 3900 ttatttgcgg ttttaccagc atatcgccaa agcattagat cattacggct attgcagtat 3960 attacacatt ttgactgcgg aagacgagga gcagttgcag ctgccgcggg tgtataatga 4020 acggaaagta gacggtttca ttatgcttgg acaagtaaat aaagaatatg cctcgctttt 4080 tcaacaaatt gagctgcctg tcgtatttct tgatttctat catgaccaag atgttgactg 4140 cgtgttaacg gataactttt ttggtatgta tgagttgact aatcatttaa ttcaatgtgg 4200 ccatcgggaa attgcttttg tcgggaatat ttactctaca agcagcattc aagaccgttt 4260 tttaggctac tataaatcat tgttggaaca tcgtattcct ttacgagagg aatatatcat 4320 tagcgaccgt gatgaaaaag ggaaatatat tgatttgtat tgccgaaaaa catgccgact 4380 gcttttgtat gcaactgtga tcaagtagcc tatcatttgg ttcatacgct caaagagtta 4440 gggtaccgtg ttcccgaaga ttgttctgtt gtaggatttg ataatgacat ttatgccaca 4500 ttggcggagc taacttaacg actgtacaag tgaatatgga agaaatgtcc gatttggctg 4560 taaaaatgat catcgagaaa agtaaagacc ggaataaaaa gtatggcagg gttttagtga 4620 aaggacaaat tatttatcgg actctgtcca acgtataaat gattgagatg gcataacaaa 4680 aagcggaggg ggatagctgt gccgattcaa gtaaatgagg aaacaaaaga atttcattta 4740 tttaatgaga atatcagtta tatttttcgg gtgctagaaa aaagcaacca attggaacat 4800 ttgtactatg gaaagcgaat caaacatcgt gattcttttc agcatctcat tgaacgggaa 4860 gtacggccgt cttgcaatat gtttgaaggc gactatactt catcgcttga acatattaaa 4920 caggaatatc caagctacgg aacgacggac tatcgctatc cgtcctatgt gatcatgaat 4980 gatatcggga gcaccgtgac gaatttccaa tatgatagtt atcgaattat tgctggaaag 5040 ccgaaacttc cttttctgcc ggctacatat gtggagaacc cagaagaagc agatacattg 5100 gaagtgacat taatcgacga tgtcctacag tgtaagttgg ttttaagcta tacgatttat 5160 cgtcatcgca atgttatttg tcgaaatgcc aaattagtga atgaagggga aaaggcgttt 5220 tatatccacc atgccatgag cagcagtgtt gattttccgg acagtgattt tgaaatggtg 5280 cagttatcgg gagcatgggc aagagagttt catttggaaa caaaaaaaat aacaaaggga 5340 atccagtctg tatatagtgt tcgcggggca agcagtcata ttcataaccc atttcttgct 5400 ttaaaacgac cggatgcaac ggaacataag ggggaagttt acggttttag tcttgtctac 5460 agcggaaatt tcttggcgca agtcgaagtc gatacgtatg atgtgacccg cgtgctaatg 5520 ggcattcatc catttcagtt tcgttggaag ctgtatccga ccgaatcgtt tcaaacgccc 5580 gaatgtgtga tggtttattc cgatcaagga ttaaatggaa tgagccaaac gtatcacgat 5640 ttataccgta cccatttagt aagaggacca tggcgggatc gtcctcgccc tattttaatt 5700 aataactggg aagcgaccta ttttgatttt aatgaagaaa aaattttgca aatcgctaaa 5760 acggcaaaag aacttggaat agaattgttc gtgttggacg atggctggtt tggcaatcgg 5820 aatgatgata caagctcgct gggcgactgg tttgaaaata gggagaaact cccaagtggt 5880 attaaaggat tatcggaaaa aattgaagcg ttagggatga agttcggttt atggtttgaa 5940 ccagaaatgg tttgtaaaga cacaaaactg ttccaagaac atcccgattg ggtgattgct 6000 acaccgaacc gccgcatgtc ccatggcaga aatcaatttg ttttggattt ttccaaacag 6060 gaagtggtag attatatttt cggcttaatg agtcacgtaa tagaaaatgc aaagatctct 6120 tatattaagt gggatatgaa tcggtatata actgaaccat actccaatgg attgcctgcc 6180 gaccgacaag gggaagtatt ccatcgttat atattaggag tttaccaatt atacgaacga 6240 ttgttggaag cgtttcctca cattttattt gaatcctgtg cgggtggtgg cggaaggttt 6300 gatccaggga tgctatatta cgcaccgcaa gcatggacga gtgatgacac cgatgcggtg 6360 gaacggctga aaattcaata tggtgcttcc ctcgtatacc cgttaagttc gattggcagc 6420 catgtttcag ctgttccaaa tcatcaagtt ggacggataa ctccgcttga cacaagagct 6480 aatgttgctt atttcggaac gtttggatat gagttggata ttacgaaatt aacggacgaa 6540 gaaaaagaaa aggtgaagca gcaagtcacc ttttttaaag aaaagagaca acttattcat 6600 cacggccgtt tttatcggct gcttgacccg ttccactcga atgaagtggc atggatggtt 6660 gtatcagaag accaaaaaga agccatcgta ggctattatc aagtactggc caaaccaaat 6720 gatcgttatc accgtctcaa gttaaaggga ttgcatccgg atttccttta tgttgtagaa 6780 ggaaaaggga ctacccatta cggcgatgag ctgatgaacg ttgggatgat ttttgctgag 6840 aattacacag accgtgccca tgaatattgg cagcgtgaga agaaaggcga ttttgcttcg 6900 caagttttcg tgttgaaggc aatatcttaa gtttatacgg aagggacacc gtcatttttg 6960 ctttcgttgt ggaaaaacgg aaagaaaccg tttcgcttcg atgaatgata atgaatcagg 7020 tagcacgggg gtggggatac gatgaaacat tatatggtat ttgatattgg cggcacccat 7080 gtaaaacatg ccgtcatgaa tgagacaggc agttttttag aaaagggaag ttacccttcc 7140 gaacgaaatg attttgagaa atttaaagaa gatttgctgg ctgtcgtacg gcaggcaaag 7200 gagcgttatg aagtggtggg aattgcaatg agctgcctgg cggagtggat agtgacagcg 7260 gtgtgatcgg agggagcagt gctcttcctt gcattcatgg cccgaatttt aaagaagtat 7320 ttgggagtga aaccggtctt ccggtggaat tggaaaatga gccgaattgc gcggcccttg 7380 gggagctttg gaaaggagcg gcaaacataa tcgagatgtt ttatttgtcg tagtaggaac 7440 gggaatcggc ggagcggttg tcaaagaccg aaaaattcat aaaggtgccc acctgcatgg 7500 cggggagttt ggttatatga ttatggatgt ccgcaacgaa aacgggaata tagaatgcaa 7560 aacatggagc gacattggtg cgacccggag cgcttgatag ccgtgttgct agaagaaaaa 7620 gagagttagc gtccatgagc taaacggtaa aaggtttttg cgatgatgga acaaggggac 7680 ctgcag 7686 // |
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