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EBI DbfetchID AF030511; SV 1; linear; genomic DNA; STD; PRO; 7004 BP. XX AC AF030511; XX DT 06-JAN-1999 (Rel. 58, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Actinobacillus pleuropneumoniae MRP ATPase homolog (mrp) gene, partial cds; DE ApxIVA var3 (apxIVA) gene, complete cds; and beta-galactosidase (lacZ) DE gene, partial cds. XX KW . XX OS Actinobacillus pleuropneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. XX RN [1] RP 1-7004 RX PUBMED; 10463177. RA Schaller A., Kuhn R., Kuhnert P., Nicolet J., Anderson T.J., MacInnes J.I., RA Segers R.P., Frey J.; RT "Characterization of apxIVA, a new RTX determinant of Actinobacillus RT pleuropneumoniae"; RL Microbiology (Reading, Engl.) 145(Pt 8):2105-2116(1999). XX RN [2] RP 1-7004 RA Frey J., Kuhn R.; RT ; RL Submitted (20-OCT-1997) to the EMBL/GenBank/DDBJ databases. RL Inst. for Veterinary Bacteriology, University of Bern, Laenggasstrasse 122, RL Bern, BE CH-3012, Switzerland XX FH Key Location/Qualifiers FH FT source 1..7004 FT /organism="Actinobacillus pleuropneumoniae" FT /strain="HV114" FT /mol_type="genomic DNA" FT /note="serotype 3" FT /db_xref="taxon:715" FT CDS <1..453 FT /codon_start=1 FT /transl_table=11 FT /gene="mrp" FT /product="MRP ATPase homolog" FT /note="similar to E.coli MRP ATPase" FT /db_xref="InterPro:IPR019591" FT /db_xref="UniProtKB/TrEMBL:Q9S547" FT /protein_id="AAD01903.1" FT /translation="IDMPPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAVKGISMFQ FT KVSVPVLGIIENMSVHICQNCGHHEDIFGTGGAEKVAKKYGTKVLGQMPLHIRLRQDLD FT AGTPTVVAAPEHDTSRAYIELAAKVASELYWQGSVIPSEIMIREVK" FT stem_loop 496..537 FT RBS 640..645 FT /note="RBS for ORF1-IV3" FT CDS 650..1120 FT /codon_start=1 FT /transl_table=11 FT /product="ORF1-IV3" FT /note="unknown protein" FT /db_xref="UniProtKB/TrEMBL:Q9ZIE0" FT /protein_id="AAD01904.1" FT /translation="MKIKKRYIALLALGSVIGYAWYQNYQWEQLMLSGYCEKDGSYCDD FT RHTKQELIDRAINYVLENQIQQTYEGDDLVDIKQYSTIEEFKKLNPNCCKVDSWPDDAV FT REDADLQREGKAYKYVKVKYLRTYLANREPEQWENYIVFDNCSGIKERHQLY" FT RBS 1134..1138 FT /gene="apxIVA" FT CDS 1146..5714 FT /codon_start=1 FT /transl_table=11 FT /gene="apxIVA" FT /product="ApxIVA var3" FT /note="RTX ApxIVA protein; ApxIVA var3 similar to FT iron-regluated FrpC protein of Neisseria meningitidis" FT /db_xref="GOA:Q9ZID9" FT /db_xref="InterPro:IPR001343" FT /db_xref="InterPro:IPR011049" FT /db_xref="InterPro:IPR013550" FT /db_xref="InterPro:IPR018511" FT /db_xref="InterPro:IPR018512" FT /db_xref="UniProtKB/TrEMBL:Q9ZID9" FT /protein_id="AAD01905.1" FT /translation="MTKLTIQDVTNLYLYKQRTLPTDRLDDSLISKTGKGENIDKKEFM FT AGPGRFVTADNFSVVKDFFTAKDSLINLSLQTRILANLKPGKYSKAQILEMLGYTKNGE FT KVDGMFTGEVQTLGFYDDGKGDLLERAYIWNTTGFKMSDNAFFVIEESGKRYIENFGIE FT PLGKQEDFDFVGGFWSNLVNRGLESIIDPSGIGGTVNLNFTGEVETYTLDETRFKAEAA FT KKSHWSLVNAAKVYGGLDQIIKKLWDSGSIKHLYQDKDTGKLKPIIYGTAGNDSKIEGT FT KITRRIAGKEVTLDIANQKIEKGVLEKLGLSVSGSDIIKLLFGALTPTLNRMLLSQLIQ FT SFSDSLAKLDNPLAPYTKNGVVYVTGKGNDVLKGTEHEDLFLGGEGNDTYYARVGDTIE FT DADGKGKVYFVREKGVPKADPKRVEFSEYITKEEIKEVEKGLLTYAVLENYNWEEKTAT FT FAHATMLNELFTDYTNYRYEVKGLKLPAVKKLKSPLVEFTADLLTVTPIDENGKALSEK FT SITVKNFKNGDLGIRLLDPNSYYYFLEGQDTGFYGPAFYIERKNGGGSKNNSSGAGNSK FT DWGGNGHGNHRNNASDLNKPDGNNGNNQNNGSNQDNHSDVNAPNNPGRNYDIYDPLALD FT LDGDGLETVSMNGRQGALFDHEGKGIRTATGWLAADDGFLVLDRNQDGIINDISELFSN FT KNQLSDGSISAHGFATLADLDTNQDQRIDQNDKLFSKLQIWRDLNQNGFSEANELFSLE FT SLNIKSLHTAYEERNDFLAGNNILAQLGKYEKTDGTFGQMGDLNFSFNPFYSRFTEALN FT LTEQQRRTINLTGTGRVRDLREAAALSEELAALLQQYTKGSDFQAQRELLPAILDKWAA FT TDLQYQHYDKTLLKTVESTDSSASVVRVTPSQLSSIRNVKHDPTVMQNCEQSKAKIATL FT NSLYGLNIDQLYYTTDKDIRYITDKVNNMYQTTGELGYRSLLLQTRVKKYVYSVNAKQF FT EGKWVADYSRTEALFNSTYKQSPENVLYDLREYLSFYNDPTEWKEGLLLLSRYIDYAKA FT QGFYENWAATSNLTIARLREAGVICAESTDLKGDEKNNIVLGSQKDNNLSGSAGDDLLI FT GGEGNDTLKGSYGADTYIFSKGHGQDVIYEYSDSANSKKDIDTLKFTDVNYAEVKFRRV FT DNDLMLFGYHDTDSVTVKSFYSHVDYQFDKLEFADRSITRDELIKAGLHLYGTDGNDDI FT KDHADWDSIVEGGKGNDILRGGYGADTYIFSKGHGQDIVYEDTNNDNRARDIDTLTFTD FT VNYAEVKFRRVDNDLMLFGYHDTDSVTIKSFYNHVDYQCDKLDFADRSITRDELGKQGM FT ALFGTDGDDNINDWGRNSVIDAGAGNDTVNGGNGDDTLIGGKGNDILRGGYGADTYIFS FT KGHGQDIVYEDTNNDNRARDIDTLKFTDINLSELWFSRENNDLIIKSLLSEDKVTVQNW FT YSHQDHKIENIRLSNEQMLVSTQVEKMVESMAGFAQKHGGEISLLSPEEVKQYINSLTA FT AL" FT stem_loop 5725..5758 FT CDS complement(5832..>7004) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /note="LacZ" FT /db_xref="GOA:Q9ZID8" FT /db_xref="InterPro:IPR004199" FT /db_xref="InterPro:IPR006102" FT /db_xref="InterPro:IPR011013" FT /db_xref="InterPro:IPR014718" FT /db_xref="UniProtKB/TrEMBL:Q9ZID8" FT /protein_id="AAD01906.1" FT /translation="IDNQIVIHNYLDFTDLADYLTIDYEFVENGVVTSGGNLSVSCQPH FT GSVVLPIELPKNNGHLWLLNLNYRLNTAVELLDEAHSLGFEQLNLFSENKLVLPQFTIE FT NSAFEVQEDRFHINVHNGQFSYQLDKQKGIFTRIEKAGKAIIQQSLDFNIWRAPTDNDR FT LIREACQNAGYDKAYTRAYEIQWQQSEQAVEFSVKSAIVSISRGRILTLDIRYRIFNDG FT QLSVEINAIRPIELPYLPRFGLRFFLAKTENAIEYFGYGEQESYIDTRNLPQLGIYRIT FT AKQNHTNYVKPQETGSHYGCEYLKSENLFVSANQPFSFNLSPYTQEELTERKHYYELQE FT SDYSVLCIDSHNEWYRFQTHAAPNLKDQYRLMESEFKIRFDLIFDKTIRQ" XX SQ Sequence 7004 BP; 2253 A; 1171 C; 1586 G; 1994 T; 0 other; atcgatatgc cgccgggtac gggcgatatc caacttactc tttcgcaaca aattccggtt 60 accggtgcgg tagtggtaac tactccgcaa gatattgcgt tattagatgc ggtgaaaggt 120 atttcaatgt tccaaaaagt gtcggtaccg gtcttaggta tcattgaaaa tatgagcgtg 180 catatctgcc aaaattgcgg tcaccacgaa gatattttcg gcaccggcgg tgcggagaaa 240 gtggcgaaga aatacggtac taaagtatta ggacaaatgc cgttgcatat tcgcttacgt 300 caagatttgg atgccggcac accgaccgtc gttgcggcac cggaacacga caccagcaga 360 gcctatattg aattagcggc aaaagtcgct tcggaattat actggcaagg ttcggttatc 420 ccgtctgaaa ttatgattcg tgaagtaaaa taagcctaca taaccacgga ataccagata 480 acacagaagg aaaacaagcg gtagaatttg cagaaaaagt tgcaaattct accgcttttt 540 tattagtacg attcgctgtt ggactgccat ttgatttggt ttgtcaggat attatgttat 600 tgtaatgaaa tgttagtgaa ttatttttat taatttgaaa ggagacaaaa tgaaaataaa 660 aaaacgttac attgcgctgc tagctttagg cagtgttatt ggctatgcct ggtatcaaaa 720 ttatcaatgg gaacagttga tgttaagtgg ctattgtgaa aaggacggaa gctattgtga 780 tgataggcat acgaagcagg aactgattga tagggcaatt aactatgtgc tggaaaatca 840 aattcaacag acatatgaag gtgatgacct tgtggatata aaacaatatt caacaataga 900 ggaatttaaa aaactaaatc cgaattgttg taaggtagat tcttggccgg atgatgctgt 960 tcgtgaggat gctgatttac agcgagaggg caaagcgtat aaatacgtaa aagtcaaata 1020 tttaagaacc tatttagcga atagagaacc tgaacaatgg gaaaattaca tagtatttga 1080 taattgcagt ggaattaaag aaagacacca actgtattaa aaatagatta gatggagaca 1140 acacgatgac aaaactaact atccaagatg tgactaattt atatttatat aagcaaagaa 1200 ctttacctac ggataggtta gatgattcgc ttattagcaa aacaggaaaa ggggaaaata 1260 ttgataaaaa ggaatttatg gcggggccgg gacgttttgt gacggccgat aattttagtg 1320 ttgtaaaaga cttttttact gcaaaggatt cattaataaa cctaagcttg cagactcgta 1380 tattagcgaa tttaaagccg ggcaaatatt ccaaagcgca gatattagaa atgttgggct 1440 atacgaaaaa tggagaaaag gtagatggca tgtttaccgg tgaagtccag acattaggct 1500 tttatgacga tggcaaaggg gatttactcg aacgcgccta tatctggaat accacaggat 1560 ttaaaatgag cgacaatgcc ttttttgtta tagaagaatc aggcaaacgc tatattgaaa 1620 actttggtat tgaacctctt ggtaagcaag aagattttga ttttgtcggc ggcttttggt 1680 ctaacttagt gaatcgtggt ttggaaagta ttatcgaccc atccggtatc ggtggaacgg 1740 taaaccttaa ctttaccggc gaggtggaaa cctacacgtt agacgaaaca aggtttaaag 1800 cggaagcggc gaagaaaagc cattggagtt tagtgaatgc ggcgaaagta tacggcggtt 1860 tagaccaaat tattaaaaaa ctatgggaca gtggctcaat taagcattta tatcaagata 1920 aagatacggg caaattaaaa ccgattattt acggcacggc cggcaacgac agtaagattg 1980 aaggcactaa aatcacccgt aggattgcgg gtaaagaagt tacgcttgat attgccaatc 2040 agaaaattga aaaaggcgtg ttagagaaat tggggctgtc tgttagtggt tcggatatca 2100 ttaaattgtt gtttggagca ttgactccaa ctttaaatag aatgttgcta tcacaactta 2160 tccagtcttt ttccgatagc ttggctaaac ttgataatcc cttagcccct tacactaaaa 2220 atggcgtggt ttatgtcacc ggcaaaggga atgatgtgct taaaggaact gaacatgagg 2280 atttgtttct cggtggtgag gggaatgata cttattatgc gagagtaggc gatacaattg 2340 aagacgccga cggcaaaggt aaagtctatt ttgtgagaga aaaaggggta cctaaggcgg 2400 atcctaagcg ggtagagttt agcgagtaca taacgaaaga agaaataaaa gaggttgaaa 2460 aggggttatt aacttacgca gttttagaaa attataattg ggaagagaaa acggcgactt 2520 tcgctcatgc gactatgctt aatgagcttt ttactgatta tactaattat cgttatgaag 2580 ttaaaggact aaaattgccc gccgttaaaa agttaaaaag tccgttggtg gagtttacag 2640 ctgatttatt aactgttacg cctattgacg aaaacggaaa agcacttagc gaaaaaagta 2700 ttacggttaa aaattttaaa aatggtgatt taggaataag gttgttggat cctaatagct 2760 attattattt ccttgaaggc caagatacgg gtttttatgg tcctgctttt tatattgaac 2820 gaaaaaacgg tggaggctct aaaaataact cgtcgggagc aggaaatagc aaagattggg 2880 gcgggaacgg gcatggaaat caccgaaata atgcctccga cctgaataaa ccggacggaa 2940 ataatgggaa taaccaaaat aacggaagca atcaagataa tcatagcgat gtgaatgcgc 3000 caaataaccc gggacgtaac tatgatattt acgatccttt agctttagat ttagatggag 3060 atgggcttga aaccgtgtcg atgaacgggc gacaaggcgc gttattcgat catgaaggaa 3120 aaggtattcg taccgcaacg ggctggctcg ctgcggatga cggtttttta gtgttagatc 3180 gtaaccaaga cggcattatt aatgatataa gcgagttatt tagtaataaa aatcaacttt 3240 ccgacgggag tatttctgca cacggttttg cgacattagc cgatttggat acaaaccaag 3300 atcagcgtat cgaccaaaat gataagctgt tttctaaact ccaaatttgg cgggatttaa 3360 atcaaaacgg ttttagtgaa gcgaatgagc tgtttagctt agaaagtttg aatattaaat 3420 ctttacatac cgcctatgaa gagcgtaatg attttctagc gggcaataat atccttgctc 3480 agcttgggaa gtatgaaaaa acggacggta cttttggaca aatgggcgat ttaaatttca 3540 gttttaaccc gttttatagc cgatttaccg aagcgttaaa tttaaccgag caacaacgtc 3600 gcacaattaa tctaaccggc accggtcggg ttcgggattt gcgtgaagcc gccgcacttt 3660 ctgaggagtt ggctgcttta ttacaacagt acactaaggg ctccgatttt caggcacaac 3720 gagaattatt gcctgccatt ttagataaat gggcggcaac ggatttacag tatcaacatt 3780 atgataaaac attacttaaa acggtagaaa gtaccgatag tagtgcttct gtcgttagag 3840 tcacgccttc tcaattaagt agtatacgca atgtaaagca tgatcctacc gttatgcaaa 3900 actgtgaaca aagtaaggca aaaattgcga ctttaaattc gctctacggg ttaaatattg 3960 atcaacttta ttatacgacg gataaagaca ttcgttatat tactgacaaa gtgaataata 4020 tgtatcaaac aaccggagaa ctcggctatc gttctttact tttacaaacg cgtgtgaaga 4080 aatatgttta tagcgttaat gcgaaacaat tcgaagggaa atgggtagcc gattattctc 4140 gtactgaagc cttatttaac tctacttata aacaatcgcc cgaaaatgta ttatatgatt 4200 tacgcgaata cctttctttc tataacgacc ctacggaatg gaaagaaggg ctattactgt 4260 taagccgtta tatagattat gctaaagcac aaggatttta tgaaaactgg gcggctactt 4320 ctaacttaac tattgcccgt ttaagagagg ctggagtaat ttgtgcagaa tcgacggatt 4380 taaaaggcga tgaaaaaaat aatattgtgt taggtagcca aaaagataat aacttatcgg 4440 gtagtgcagg tgatgatcta cttatcggcg gagagggtaa tgatacgtta aaaggcagct 4500 acggtgcaga cacctatatc tttagcaaag ggcatggaca agatgtaatt tatgaatatt 4560 ccgacagtgc aaactctaaa aaagatattg ataccttaaa atttaccgat gtgaattatg 4620 cggaagtgaa gtttcgacga gtagataatg acttaatgtt attcggttat catgatacgg 4680 attcggtcac ggtaaaatcc ttctacagcc atgtagatta tcaatttgac aaattggagt 4740 ttgctgaccg cagtataact cgcgatgaac tgattaaagc agggcttcat ctatacggca 4800 ccgatggcaa tgatgatata aaggatcatg cggattggga cagcattgtg gaaggcggca 4860 aaggcaacga tattctaaga ggtggctacg gtgcggacac ctatatcttt agcaaaggac 4920 acggacagga tatcgtttat gaagatacca ataatgataa ccgagcaaga gatatcgaca 4980 ccttaacatt tactgatgtg aattatgcgg aagtgaaatt ccgacgagta gataatgact 5040 taatgttatt cggttatcat gatacggatt cggtcacgat aaaatccttc tacaaccatg 5100 tagattatca atgtgacaaa ttggactttg ctgaccgcag tataactcgt gatgaactag 5160 gtaaacaagg tatggcatta tttggcactg acggcgatga taatatcaac gactggggac 5220 gtaactcggt gattgatgcc ggtgcgggta atgatacggt taatggcggt aatggcgatg 5280 acaccctcat cggcggcaaa ggtaatgata ttctaagagg tggctacggt gcggacacct 5340 atatctttag caaaggacac ggacaggata tcgtttatga agataccaat aatgataacc 5400 gcgcaagaga tatcgacacc ttaaaattta ctgatattaa tttatccgaa ctttggttta 5460 gccgagaaaa taacgatttg attattaaat cattattaag tgaggataaa gtcacggttc 5520 aaaattggta ttcacaccaa gatcataaaa tagaaaatat tcgtttatcg aatgagcaaa 5580 tgttggtgag cactcaggtg gagaagatgg ttgagtcgat ggccggcttt gctcagaagc 5640 acggaggaga gatatctctt ctgtcgcctg aagaggtaaa acaatatatc aatagcttaa 5700 cagctgcttt ataacatacg aaagaaatcg gcacagtttt tgtgaactgt gccgatttga 5760 ttttagtgta agaatatagc ctgattttaa gaaatttact cttggctaat aactatttcc 5820 cattttataa gttattgacg gatggtttta tcaaatatga gatcaaatct tattttaaat 5880 tcgctttcca ttaagcgata ttgatctttt aagtttgggg ccgcatgagt ttggaaccga 5940 taccactcat tgtgggaatc aatacacaat acgctgtaat cggactcttg cagttcataa 6000 taatgctttc tctccgttaa ttcttcttgc gtatatggcg agagattaaa gctgaatggc 6060 tggttcgcac taacaaacag gttctccgat ttcagatatt cacaaccgta atggctaccg 6120 gtttcctgcg gttttacata attggtatga ttttgtttag ctgttatacg gtagatgcct 6180 aattgtggta aattgcgtgt gtcaatatag ctttcttgtt ctccgtaacc gaaatactca 6240 atggcgtttt ctgttttagc taagaagaaa cgtaagccga agcggggtaa atacggtaat 6300 tcgatcgggc gaatagcgtt aatttcaacc gaaagttgtc cgtcattgaa gatacgataa 6360 cgaatatcca gtgttaaaat gcgaccgcga gaaattgaca caattgcaga ttttactgaa 6420 aattcgaccg cttgttcgct ttgctgccac tgaatttcat acgctctggt ataggcttta 6480 tcgtagccgg cattttggca cgcctcacga atgaggcgat cattgtcggt tggcgcacgc 6540 caaatattaa aatctaacga ttgttggata atcgctttac cggctttttc aatacgggtg 6600 aaaatccctt tctgtttatc taattgataa ctaaattgac cgttgtgtac gttaatgtgg 6660 aagcgatctt cttgtacttc aaatgcactg ttctcaattg tgaattgtgg taatactaat 6720 ttattttcgc taaataaatt gagctgctcg aagccaagtg aatgtgcttc gtctaataat 6780 tcgaccgcgg tatttaagcg ataatttaaa ttcagtagcc ataaatgccc gttatttttt 6840 ggtaactcaa tcggtaatac tacgctgccg tgcggttggc aagaaacgga taaattccca 6900 ccgcttgtca ccacgccgtt ttcgacaaat tcgtaatcaa tcgttaaata atcggcaaga 6960 tcagtgaaat ccaagtagtt gtggatcaca atttggttat cgat 7004 // |
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