 |
|
EBI DbfetchID AF029858; SV 1; linear; mRNA; STD; PLN; 1929 BP. XX AC AF029858; XX DT 13-JAN-1998 (Rel. 54, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Sorghum bicolor cytochrome P450 CYP71E1 (CYP71E1) mRNA, complete cds. XX KW . XX OS Sorghum bicolor (sorghum) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; PACCAD clade; OC Panicoideae; Andropogoneae; Sorghum. XX RN [1] RP 1-1929 RX DOI; 10.1104/pp.115.4.1661 RX PUBMED; 9414567. RA Kahn R., Bak S., Svendsen I., Halkier B.A., Moller B.L.; RT "Isolation and reconstitution of cytochrome P450ox and in vitro RT reconstitution of the entire biosynthetic pathway of the cyanogenic RT glucoside dhurrin from sorghum"; RL Plant Physiol. 115(4):1661-1670(1997). XX RN [2] RP 1-1929 RX DOI; 10.1023/A:1005915507497 RX PUBMED; 9484480. RA Bak S., Kahn R.A., Nielsen H.L., Moller B.L., Halkier B.A.; RT "Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from RT Sorghum bicolor (L.) Moench by a PCR approach and identification by RT expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome RT P450 in the biosynthesis of the cyanogenic glucoside dhurrin"; RL Plant Mol. Biol. 36(3):393-405(1998). XX RN [3] RP 1-1929 RA Bak S., Kahn R.A., Nielsen H.L., Moller B.L., Halkier B.A.; RT ; RL Submitted (15-OCT-1997) to the EMBL/GenBank/DDBJ databases. RL Department of Plant Biology, The Royal Veterinary and Agricultural RL University, 40 Thorvaldsensvej, Frederiksberg C DK-1871, Denmark XX FH Key Location/Qualifiers FH FT source 1..1929 FT /organism="Sorghum bicolor" FT /strain="SS1000" FT /mol_type="mRNA" FT /tissue_type="1-2 cm high etiolated seedlings" FT /db_xref="taxon:4558" FT CDS 81..1676 FT /codon_start=1 FT /gene="CYP71E1" FT /product="cytochrome P450 CYP71E1" FT /function="second multifunctional cytochrome P450 in the FT biosynthetic pathway of the cyanogenic glucoside dhurrin. FT Catalyzes the conversion of p-hydroxyphenylacetaldoxime to FT p-hydroxymandelonitrile" FT /note="No EST#s identified." FT /db_xref="GOA:O48958" FT /db_xref="HSSP:1DT6" FT /db_xref="InterPro:IPR017973" FT /db_xref="UniProtKB/Swiss-Prot:O48958" FT /protein_id="AAC39318.1" FT /translation="MATTATPQLLGGSVPQQWQTCLLVLLPVLLVSYYLLTSRSRNRSR FT SGKLGGAPRLPPGPAQLPILGNLHLLGPLPHKNLRELARRYGPVMQLRLGTVPTVVVSS FT AEAAREVLKVHDVDCCSRPASPGPKRLSYDLKNVGFAPYGEYWREMRKLFALELLSMRR FT VKAACYAREQEMDRLVADLDRAAASKASIVLNDHVFALTDGIIGTVAFGNIYASKQFAH FT KERFQHVLDDAMDMMASFSAEDFFPNAAGRLADRLSGFLARRERIFNELDVFFEKVIDQ FT HMDPARPVPDNGGDLVDVLINLCKEHDGTLRFTRDHVKAIVLDTFIGAIDTSSVTILWA FT MSELMRKPQVLRKAQAEVRAAVGDDKPRVNSEDAAKIPYLKMVVKETLRLHPPATLLVP FT RETMRDTTICGYDVPANTRVFVNAWAIGRDPASWPAPDEFNPDRFVGSDVDYYGSHFEL FT IPFGAGRRICPGLTMGETNVTFTLANLLYCYDWALPGAMKPEDVSMEETGALTFHRKTP FT LVVVPTKYKNRRAA" XX SQ Sequence 1929 BP; 374 A; 680 C; 580 G; 295 T; 0 other; cagagctaga agcagctcac actccacact cgtctcgccc ggccataccc caaggcaagc 60 aggggcacgg gcaattaaca atggccacca ccgccacccc gcagctcctc ggcggcagcg 120 tgccgcagca gtggcagacg tgcctcctgg tgctcctccc tgtgctgctg gtgtcctact 180 acctcctcac cagcaggagc aggaacagga gcaggagcgg caagctgggc ggggcgccgc 240 ggctgccgcc gggccctgcg cagctgccga tcctgggcaa cctgcacctg ctgggcccgc 300 tgccgcacaa gaacctccgc gagctggcgc ggcggtacgg ccccgtgatg cagctccgtc 360 taggcaccgt gccgacggtg gtggtgtcca gcgcggaggc ggcgcgggag gttctcaagg 420 tgcacgacgt cgactgctgc agccggccgg cgtcgcccgg tcccaagcgc ctctcctacg 480 acctcaagaa cgtcggcttc gcgccctacg gcgagtactg gcgcgagatg cgcaagctct 540 tcgcgctcga gctcctcagc atgcgccgcg tcaaggccgc ctgctacgcg cgcgagcagg 600 agatggacag gctcgtcgcc gacctcgacc gcgccgccgc gtccaaggcc tccatcgtcc 660 tcaacgacca cgtcttcgcc ctcaccgacg gcatcatcgg caccgtcgcg ttcggcaaca 720 tctacgcctc caagcagttc gcgcacaagg agcgcttcca gcacgtgctg gacgacgcca 780 tggacatgat ggccagcttc tccgccgagg acttcttccc caacgccgcc ggccgcctcg 840 ccgaccgcct ctcgggcttc ctcgcccgcc gcgagcgcat cttcaacgag ctcgacgtct 900 tcttcgagaa ggtcatcgac cagcacatgg acccggcgcg ccccgtgccg gacaacggcg 960 gcgacctcgt cgacgtcctc atcaacctgt gcaaggagca cgacggcacg ctccgcttca 1020 ccagggacca cgtcaaggcc atcgtcctcg acaccttcat cggcgccatc gacaccagct 1080 ccgtcaccat cctgtgggcc atgtcggagc tgatgcggaa gccgcaggtg ctgaggaagg 1140 cgcaggccga ggtgcgggcc gccgtgggcg acgacaagcc gcgcgtcaac tcggaagacg 1200 ccgccaagat cccgtacctg aagatggtgg tcaaggagac gctgcggctg cacccgccgg 1260 cgacgctgct ggtgccccgg gagacgatgc gggacaccac catctgcggc tacgacgtgc 1320 cggccaacac gcgcgtcttc gtcaacgcct gggccatcgg cagggacccg gcgagctggc 1380 cggcgcccga cgagttcaac ccggaccgct tcgtggggag cgacgtcgac tactacggct 1440 cgcacttcga gctcataccg ttcggggccg gccgccggat ctgcccggga ctcaccatgg 1500 gcgagaccaa cgtcaccttc accctcgcca acctgctcta ctgctacgac tgggcgctgc 1560 cgggggccat gaagccggag gacgtcagca tggaggagac cggagcgctc acgttccacc 1620 ggaagacgcc gcttgtggtg gtgcccacca aatacaagaa ccgccgcgcc gcctagtgag 1680 cagagccgag cagagcaatg gtcgacgacg acgacgacga cgactgaata agcgtgccaa 1740 agtttagtac tacgtacgta cgtacctact gctactacgt acagctagcc aacagtcaga 1800 gttggacact gttggagcta tcatccggtc ctcttctttt tgtgatacgt atttgttatg 1860 tgttttagtg ccgcaaagca caaaagaaat aaagcccatc acagtcgcga gtcaaaaaaa 1920 aaaaaaaaa 1929 // |
|