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EBI DbfetchID AF027867; SV 1; linear; genomic DNA; STD; PRO; 2431 BP. XX AC AF027867; XX DT 14-NOV-1997 (Rel. 53, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Streptomyces achromogenes SacI methylase (sacIM) and SacI restriction DE endonuclease (sacIR) genes, complete cds. XX KW . XX OS Streptomyces achromogenes OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RP 1-2431 RX DOI; 10.1007/s004380050890 RX PUBMED; 9862476. RA Xu S.Y., Xiao J.P., Ettwiller L., Holden M., Aliotta J., Poh C.L., RA Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., RA Slatko B., Benner J.; RT "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI RT restriction-modification systems in Escherichia coli"; RL Mol. Gen. Genet. 260(2-3):226-231(1998). XX RN [2] RP 1-2431 RA Xu S.-y.; RT ; RL Submitted (02-OCT-1997) to the EMBL/GenBank/DDBJ databases. RL Research, New England Biolabs, Inc., 32 Tozer Rd., Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..2431 FT /organism="Streptomyces achromogenes" FT /mol_type="genomic DNA" FT /db_xref="taxon:67255" FT CDS 43..1215 FT /codon_start=1 FT /transl_table=11 FT /gene="sacIM" FT /product="SacI methylase" FT /function="C5 cytosine methylase; C5 methyltransferase; FT modify cytosine in the sequence of 5'GAGCTC3'" FT /db_xref="GOA:O31073" FT /db_xref="HSSP:1G55" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:O31073" FT /protein_id="AAC97118.1" FT /translation="MNHELPVISLFSGAGGLDCAIESCAEPPLVQDGSGSPLRVAVATD FT YEQTALDTLSANFPHTKTLCGDIQTIPTAELLEAGGLKPGDPTLVIGGPPCTPFSKSGF FT WIEEKRNSADPNASLLDEYVRVVRESKPEAFILENVQGLTYKTHQAQFDRLIAGLKDAG FT YNPTFRVLLAAEYGVPQLRRRVFVVGRRDGKAFHFPETTHSGESERDRVIDHTKIPFTS FT LREALAGLPDVPEAGEVVEGTYAELAAEVPPGQNYLWHTDRYGGRNEFKWRSRYWTFLL FT KADPDRPSTTLQAQPGPWVGPFHWENVKNANGEERARRFRVAEMKRIMTFPDEFVFTGV FT KREVQRQIGNPVPVELGKVVVRALMEQLGYLDSRGTTIPSQAGHEQLELI" FT CDS 1233..2309 FT /codon_start=1 FT /transl_table=11 FT /gene="sacIR" FT /product="SacI restriction endonuclease" FT /function="cleave DNA sequence 5'GAGCTC3' between the 5th FT and 6th bases to generate a 4-base 3' overhang" FT /note="SacI restriction enzyme" FT /db_xref="GOA:O31074" FT /db_xref="InterPro:IPR019066" FT /db_xref="UniProtKB/Swiss-Prot:O31074" FT /protein_id="AAC97119.1" FT /translation="MGITIKKSTAEQVLRKAYEAAASDDVFLEDWIFLATSLREVDAPR FT TYTAALVTALLARACDDRVDPRSIKEKYDDRAFSLRTLCHGVVVPMSVELGFDLGATGR FT EPINNQPFFRYDQYSEIVRVQTKARPYLDRVSSALARVDEEDYSTEESFRALVAVLAVC FT ISVANKKQRVAVGSAIVEASLIAETQSFVVSGHDVPRKLQACVAAGLDMVYSEVVSRRI FT NDPSRDFPGDVQVILDGDPLLTVEVRGKSVSWEGLEQFVSSATYAGFRRVALMVDAASH FT VSLMSADDLTSALERKYECIVKVNESVSSFLRDVFVWSPRDVHSILSAFPEAMYRRMIE FT IEVREPELDRWAEIFPET" XX SQ Sequence 2431 BP; 477 A; 662 C; 761 G; 531 T; 0 other; gatcacggtc ctcccgaggt tgcgatacgt tagggtggcg cggtgaacca tgagcttcct 60 gtgatcagtc tgttctccgg ggccggtggc ctcgactgcg ccatcgagag ctgcgctgag 120 ccaccgctgg tccaggacgg ctccgggtct ccgctacgtg tcgcggttgc cactgactat 180 gagcagaccg ctctggatac actgtcggcc aacttcccgc acaccaagac gctgtgtggg 240 gatatccaaa cgatcccgac cgcagagttg ctggaagccg gcgggctgaa gcccggcgat 300 cccaccctgg tcatcggtgg tcctccttgt acgccgttca gtaagtccgg attctggatc 360 gaggagaagc gcaacagcgc cgatcccaat gcctccctcc ttgacgagta cgtccgtgtg 420 gttcgggaga gcaagcccga agccttcatc ctggaaaatg tgcagggcct gacgtacaag 480 acccatcagg ctcagttcga ccgactcatt gcgggcctta aggatgcggg ctacaacccg 540 acgttccgtg tgctccttgc ggccgagtat ggggttccgc agctccgcag gcgagtattc 600 gttgtggggc gacgtgacgg taaggctttc cacttccccg aaaccacgca ctctggcgag 660 tcggagcggg atcgggtgat tgatcacacc aagattccgt tcacgagtct tcgcgaggca 720 cttgccggac tgccggacgt tcctgaggct ggagaagttg ttgagggaac ctacgccgaa 780 ctcgctgcgg aagttcctcc tgggcaaaac tacctatggc acaccgaccg atatggcggt 840 cgcaacgagt tcaaatggcg tagccgctat tggaccttcc tgctgaaggc ggacccagac 900 cgcccttcta cgacgctgca ggctcagcca ggtccatggg tcggaccatt tcactgggag 960 aacgtgaaga acgcgaacgg ggaagagcga gcgcgtaggt ttcgcgttgc cgaaatgaag 1020 cggatcatga ctttccctga tgagttcgtt ttcaccggag tgaagcgcga ggtgcagcgg 1080 cagatcggta atcccgtccc ggtggagttg gggaaggtcg tcgtccgggc cctgatggaa 1140 caactcggct atcttgattc tcgcggtact actattccta gccaggctgg acacgaacag 1200 cttgaattga tctagagcgg catggggggg caatgggaat aacaattaaa aagagcacgg 1260 cggagcaggt tttgaggaag gcgtacgagg cggccgcttc ggatgatgta ttcctcgaag 1320 attggatatt tctggctact tcgctacgcg aggtagatgc tccaaggact tacacggctg 1380 cgcttgtgac agcactgctc gcgcgtgcat gtgacgacag ggttgaccca cgctctatca 1440 aagagaagta cgacgatcgc gccttctctc tgcgtaccct ttgccacggt gtagtggttc 1500 cgatgtccgt agaacttgga tttgatctgg gggctacggg ccgcgagccg atcaacaatc 1560 agccattctt ccgttacgac caatacagcg agatcgtcag ggtccagacg aaggcgcgcc 1620 cctatctgga ccgagtaagc agtgcccttg ctagggtcga tgaagaagac tattccactg 1680 aagagtcgtt ccgagcgctt gtcgctgtct tggccgtgtg tatctctgtg gcgaacaaaa 1740 agcagcgcgt tgccgtcggg agtgccatcg tcgaggcgag cctaatcgct gagactcaga 1800 gcttcgttgt gagtggccac gacgttccac gaaagctgca ggcttgtgtt gcggctggtc 1860 ttgacatggt ctacagcgag gtcgtctcgc ggaggatcaa tgacccttct cgcgactttc 1920 ctggtgacgt ccaagtgatt ttggacggcg atccattgct taccgttgaa gtgcgtggaa 1980 agagcgtgtc gtgggagggg cttgagcaat tcgtcagcag tgcaacgtat gccggcttcc 2040 gacgagttgc gctcatggtc gatgcggcgt cgcacgtgtc gcttatgtcg gccgatgatc 2100 tgaccagcgc gcttgagcgg aagtacgagt gcatcgtcaa ggtgaacgag agcgtgtcga 2160 gcttcctgcg tgacgtattc gtctggtcgc ctcgcgacgt ccactcgatt ctttcggcct 2220 ttcctgaggc aatgtatcgc cgcatgatag agatcgaggt gcgggagccg gagttggatc 2280 ggtgggctga gatcttccct gaaacgtgat gtgatactgc gcggctaact tacggccggc 2340 ctgacgctta gcggcgatcg ggccggtcta tgtcgtaggc aacagaatcc cccctagatc 2400 atggagagcg cttgtcgctg tcttgggccc t 2431 // |
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