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EBI DbfetchID AF027499; SV 1; linear; genomic DNA; STD; PRO; 5591 BP. XX AC AF027499; XX DT 02-MAR-1998 (Rel. 54, Created) DT 02-JUN-2000 (Rel. 63, Last updated, Version 2) XX DE Azotobacter vinelandii mannuronan C-5-epimerase (algG) gene, partial cds; DE and AlgX, alginate lyase (algL), AlgI, and AlgV genes, complete cds. XX KW . XX OS Azotobacter vinelandii OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Azotobacter. XX RN [1] RP 1-5591 RX DOI; 10.1016/S0378-1119(99)00119-5 RX PUBMED; 10352233. RA Vazquez A., Moreno S., Guzman J., Alvarado A., Espin G.; RT "Transcriptional organization of the Azotobacter vinelandii algGXLVIFA RT genes: characterization of algF mutants"; RL Gene 232(2):217-222(1999). XX RN [2] RP 1-5591 RA Vazquez R.A.; RT ; RL Submitted (30-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL Microbiologia Molecular, Inst. de Biotecnologia. UNAM, Av. Universidad s/n RL Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico XX DR StrainInfo; 119199; ATCC 9046. XX FH Key Location/Qualifiers FH FT source 1..5591 FT /organism="Azotobacter vinelandii" FT /strain="ATCC9046" FT /mol_type="genomic DNA" FT /db_xref="taxon:354" FT CDS <1..99 FT /codon_start=1 FT /transl_table=11 FT /gene="algG" FT /product="mannuronan C-5-epimerase" FT /note="AlgG" FT /db_xref="UniProtKB/TrEMBL:O52193" FT /protein_id="AAC04565.1" FT /translation="LEDKQEEILDLLVRRQKAVLIDPVVDPAQAEL" FT CDS 120..1571 FT /codon_start=1 FT /transl_table=11 FT /gene="algX" FT /product="AlgX" FT /note="similar to Pseudomonas aeruginosa AlgX" FT /db_xref="GOA:O52194" FT /db_xref="UniProtKB/Swiss-Prot:O52194" FT /protein_id="AAC04566.1" FT /translation="MKTHNSKWIGPAALAAAIALAAAGVRAEETPTGLPVYRAESCCDL FT CPAAADPNSYTSNYMKGFVTLVQGNESDWLFRTNEDLRTEFGTTPEGYRQLKALHDAFK FT SRGVELVIVYQPTRGMVQRNKLLPADYARFDYDKAVRNFRATLKHFEQLGYWVPDLTPL FT TDEKVEPAFYFRGDHHWTSYGAERSARIVAETVKEIPAFADIPRKEFVTKKMGRMGKRG FT THHRVAGQLCNTTYAFEHSDQFFTEPKGEGGGDLFGDSSLPQITLVGTSHSGTNYNFAG FT FLSEYMGAEILNVAFPGSGLEGSMLKYLASDEFQKNPPKILIWEFSPLYDLAEDKFYRQ FT ALSMLGNACEGEKTLLAGKATLRPGEAGKEVLINGAGRLVEATNSRHQVDIRFSDPSVK FT KLEGTIWYMTGRREKFQFDKPVTTETNGRFAFNMRDEADWGGLNFFAMEIQPPEGLKEP FT VEVEVRLCKRHDYHAPANLTARSGN" FT CDS 1580..2704 FT /codon_start=1 FT /transl_table=11 FT /gene="algL" FT /product="alginate lyase" FT /note="AlgL; similar to Pseudomonas aeruginosa AlgL" FT /db_xref="GOA:O52195" FT /db_xref="InterPro:IPR008397" FT /db_xref="InterPro:IPR008929" FT /db_xref="UniProtKB/Swiss-Prot:O52195" FT /protein_id="AAC04567.1" FT /translation="MHKTRLALSCLLGSLLLSGAVHAAEALVPPKGYYAPVDIRKGEAP FT ACPVVPEPFTGELVFRSKYEGSDAARSTLNEEAEKAFRTKTAPITQIERGVSRMVMRYM FT EKGRAGDLECTLAWLDAWAEDGALLTTEYNHTGKSMRKWALGSLAGAYLRLKFSSSQPL FT AAYPEQARRIESWFAKVGDQVIKDWSDLPLKRINNHSYWAAWAVMAAGVATNRRPLFDW FT AVEQFHIAAGQVDSNGFLPNELKRRQRALAYHNYSLPPLMMVAAFALANGVDLRGDNDG FT ALGRLAGNVLAGVEKPEPFAERAGDEDQDMEDLETDAKFSWLEPYCALYSCSPALRERK FT AEMGPFKNFRLGGDVTRIFDPAEKSPRSTVGKRD" FT CDS 2897..4396 FT /codon_start=1 FT /transl_table=11 FT /gene="algI" FT /product="AlgI" FT /note="similar to Pseudomonas aeruginosa AlgI" FT /db_xref="GOA:O52196" FT /db_xref="InterPro:IPR004299" FT /db_xref="UniProtKB/Swiss-Prot:O52196" FT /protein_id="AAC04568.1" FT /translation="MVFSSNVFLFLFLPLFLGLYYLSPARYRNLLLLTASYVFYAWWRI FT DFLGLFAAVTVFNYWIGLRIGAAGVRTQSAQRWLTLGVVVDLCVLGYFKYANFGVDSLN FT AIITSFGVEPFVVTHILLPIGISFYVFESISYIIDVYRGDTPATRNLVDFAAFVAIFPH FT LIAGPVLRFRDLVDQFNHRTHTLDKFAEGCTRFMQGFIKKVFIADSLAPIADHCFALSD FT PTTGDAWLGALAYTAQLYFDFSGYSDMAIGLGLMIGFRFMENFNQPYISQSITEFWRRW FT HISLSTWLRDYLYISLGGNRGTTFQTYRNLILTMLLGGLWHGANWTFIIWGAWHGTWLA FT IERALRIDAAPKTIRPLRWVFAFLLVMVGWVIFRAENLDVAWRMYAAMFSFGDWTLSDL FT NKAQLTSLQIATLLLAYVVIAVYGIRQFYAQPLTGAPKAKANDQADAPQGIVHASSGTL FT AYSQAIDIPALATRVAVLLLFAASVLKLSAQSFSPFLYFQF" FT CDS 4409..5575 FT /codon_start=1 FT /transl_table=11 FT /gene="algV" FT /product="AlgV" FT /note="similar to Pseudomonas aeruginosa AlgJ" FT /db_xref="GOA:O52197" FT /db_xref="UniProtKB/Swiss-Prot:O52197" FT /protein_id="AAC04569.1" FT /translation="MNRTTNLVYAGTFAGTLLALSLWSLKGAAGFSTADNTPVLNGKLA FT LAFEKHYDEEFPIKKLGTNLWAALDYTLFGEGRPGVVIGANQWLFSDEEFKPTAAASQN FT ITDNQALIQGVRETLARNNVQLVMAILPAKARLYPENFGEQQPASLHEQLYQNFRRIVA FT DAGIQAPDLLGPLQQAKAGGQVFLRTDTHWTPYGAQVVAGQLATTIKPIGVLPESGNVY FT VTETLPGGPHKGDLTNFLPLDPLFEELLPPPDQLAKHNTRQQEESAPAGDDLFAETQVP FT VALVGTSYSADERWNFAGALRQALGSDLVNFAEDGRGPLLPMLKFLQSEDFKKSPPRLV FT IWEFPERYLPMAYDLSEFDADWIAQLKAAGRQDKQLADNTATNQGARH" XX SQ Sequence 5591 BP; 1051 A; 2011 C; 1640 G; 889 T; 0 other; ctcgaggaca aacaagaaga gatcctcgat ctgctggtgc gccgccagaa ggccgtgctg 60 atcgaccccg tcgtcgatcc cgcccaggcc gagctgtaga accgatgaga ctgcgcaaca 120 tgaagaccca caacagcaaa tggattggac ccgccgcgct ggccgcggcg atcgccctgg 180 ccgccgccgg ggtgcgtgcc gaagaaaccc ctaccgggct gcccgtctac cgcgccgagt 240 cctgctgcga cctgtgcccg gccgccgcgg accccaacag ctacaccagc aactacatga 300 agggcttcgt caccctggtg cagggcaacg agagcgactg gctgttccgt accaacgagg 360 acctgcgcac cgaattcggt accactccag aaggttaccg tcagctcaag gcattgcacg 420 acgccttcaa gagccgcggc gtcgagttgg tgatcgtcta ccagccgacc cgcggcatgg 480 tgcaacgcaa caaactgttg cccgccgact acgcccgctt cgactacgac aaggcggtga 540 ggaacttccg ggcgaccctg aagcatttcg agcaactcgg ctactgggtg cccgacctga 600 ctccgctgac cgacgaaaag gtcgagccgg ccttctactt ccgcggcgac caccactgga 660 cctcctacgg cgccgaacgc agtgcgcgga tcgtcgccga gaccgtgaag gagatccccg 720 ccttcgcgga catcccgcgc aaggagttcg tcaccaagaa aatgggccgc atgggcaagc 780 gcggcaccca ccaccgggtc gccggccagt tgtgcaacac cacctacgcc ttcgagcaca 840 gcgaccagtt cttcaccgaa cccaagggtg aaggcggcgg cgacctgttc ggcgacagca 900 gcctgccgca gatcaccctg gtcggtacca gccacagcgg caccaactac aacttcgccg 960 gcttcctctc cgaatacatg ggtgccgaaa tcctcaacgt cgccttcccc ggcagcggcc 1020 tagaaggctc gatgctcaaa tacctggcca gcgacgaatt ccagaagaat ccgccgaaga 1080 tcctcatctg ggaattctcg ccgctctacg acctggccga ggacaagttc taccgccagg 1140 cgctgtccat gctcggcaat gcctgcgaag gcgaaaaaac cctgctcgcc ggcaaagcca 1200 ccctgcgccc cggcgaagcc ggcaaggaag tgctgatcaa cggcgccggc cgactggtcg 1260 aggccaccaa ctcccgccat caggtggaca tccgcttcag cgacccctcg gtgaaaaagc 1320 tggaaggcac catctggtac atgaccggcc gccgcgagaa gttccagttc gacaagccgg 1380 tcaccaccga aaccaacggc cgtttcgcct tcaacatgcg cgacgaggcc gactggggcg 1440 gactcaactt cttcgccatg gaaatccagc cgccggaggg gctcaaggag cccgtcgagg 1500 tcgaggtgcg cctctgcaag cgccacgact atcacgctcc ggccaacctg accgcccgta 1560 gcggcaactg aggcctacca tgcacaagac cagactcgcc ctctcctgcc tgctcggcag 1620 cctgctgttg agcggcgccg tccacgccgc cgaagccctg gttccgccca agggctacta 1680 cgcgccggtg gatatccgca aaggcgaggc gccggcctgt ccggtggtgc ccgaaccctt 1740 caccggggaa ctggtctttc gcagcaagta cgaaggctcg gacgcggccc ggtcgaccct 1800 caacgaggaa gcggaaaagg ccttccgcac caagaccgcg ccgatcaccc agatcgagcg 1860 cggcgtcagc cggatggtca tgcgctacat ggagaaaggc cgcgccggcg acctggaatg 1920 caccctggcc tggctggacg cctgggccga agacggcgcc ctgctcacca ccgagtacaa 1980 ccacaccggc aaatccatgc gcaagtgggc cctcggcagc ctggccggcg cctacctgcg 2040 cctgaaattc tccagctcgc agccgctggc ggcctatccc gaacaggcac ggcgcatcga 2100 gagctggttc gccaaggtgg gcgaccaggt gatcaaggac tggagcgacc tgccgctcaa 2160 gcggatcaac aaccactcct actgggcggc ctgggcggtc atggccgccg gcgtggcgac 2220 caaccgccgg ccgctgttcg actgggcggt cgagcagttc cacatcgccg ccgggcaggt 2280 cgactcgaat ggcttcctgc ccaacgaact caagcgccgc caacgtgcgc tggcctacca 2340 caactacagc ttgccgcccc tgatgatggt cgccgccttc gccctggcca acggtgtcga 2400 cctgcgcggc gacaacgatg gcgccctggg ccgcctggcc ggcaacgtgc tggccggcgt 2460 cgagaagccc gaaccgttcg ccgaacgggc cggcgacgag gatcaggaca tggaggatct 2520 ggagaccgac gccaagttct cctggctgga accctactgc gcgctctact cctgctcccc 2580 cgccctgcgg gaaaggaagg ccgagatggg cccgttcaag aatttccgcc tgggtggcga 2640 cgtgacccgg atcttcgatc cggccgagaa gtcgcccaga tcgaccgtcg gcaagcgcga 2700 ctagcgcggc cccgacgtcg ccccagcagc cggcagtcag tccgtcgccg gccgccacgc 2760 ccttccgggc caggcggaat gccccgaaat gcgcaacaga cgcagccggg aacggacctc 2820 ttcggggacc ccccggggga gaggccgggc tcagagaaga gcctgaaaca ttggtgctag 2880 aagcgagaca cgggtaatgg tgttttcatc caacgtcttc ctgtttttat tcctgccgct 2940 gttcctcggg ttgtactacc tgagcccggc gcgctaccgc aacctgttgc tgctgaccgc 3000 cagctatgtc ttctatgcct ggtggcgcat cgacttcctt ggactgtttg ccgccgtcac 3060 cgtcttcaac tactggatcg gcctacgcat cggcgccgcc ggtgtgcgca cccagtccgc 3120 acaacgctgg ctgacgctcg gcgtggtggt cgacctctgt gtgctcggct acttcaagta 3180 cgcgaacttc ggcgtggaca gtctgaacgc gatcatcacc tcgttcggcg tggaaccctt 3240 cgtggtcacc cacatcctgc tgccgatcgg catctccttc tacgtcttcg aatccatcag 3300 ttacatcata gacgtttatc gcggagatac cccggcgacc cgcaatctgg tcgacttcgc 3360 ggccttcgtg gcgatcttcc cgcacctgat cgccggtccg gtgctgcgct tccgcgacct 3420 ggtcgaccag ttcaaccatc gcacccacac gctggacaag ttcgccgaag gctgcacccg 3480 cttcatgcag ggcttcatca agaaggtgtt catcgccgac agcctggcgc cgatcgccga 3540 tcactgcttc gccctcagcg atccgaccac cggcgatgcc tggctcggcg ccctggccta 3600 caccgcccag ctgtacttcg acttctccgg ctacagcgac atggccatcg gcctcggcct 3660 gatgatcggc ttccgcttca tggagaactt caaccaaccc tacatcagcc agtcgatcac 3720 cgaattctgg cgccgctggc acatcagcct gtccacctgg ctgcgcgact acctgtacat 3780 cagcctgggc ggcaaccgcg gcaccacctt ccagacctac cgcaacctga tcctcaccat 3840 gctgctcggc ggcctgtggc acggcgccaa ctggaccttc atcatctggg gcgcctggca 3900 cggcacctgg ctggcgatcg agcgcgccct gcgcatcgac gccgcgccga agaccattcg 3960 cccgctgcgc tgggtcttcg ccttcctgct ggtgatggtc ggctgggtga tcttccgcgc 4020 cgagaacctc gacgtcgcct ggcgcatgta cgccgccatg ttcagcttcg gcgactggac 4080 cctgtccgac ctgaacaagg cacaactgac cagcctgcag atcgccaccc tgctgctcgc 4140 ctacgtggtg atcgcggtgt acggcattcg ccagttctac gcccaaccgc tcaccggcgc 4200 gcccaaggcc aaggcgaacg atcaggccga cgcgccccag ggcatcgtcc atgcgtcgag 4260 cggcaccctg gcctattcgc aggccatcga tataccggca ctggcgaccc gcgtcgcggt 4320 gctcctgctg ttcgccgcct ctgtgctcaa gctttcggcg cagagtttct cgcctttcct 4380 ctacttccag ttttgaagag ggaccgccat gaatagaaca accaacctgg tctacgccgg 4440 taccttcgcc ggcaccctgc tggcgctcag cctgtggtcg ctgaaaggcg ccgccggctt 4500 ctccaccgcc gataacaccc cggtgctcaa cggcaagctg gccctggcct tcgagaaaca 4560 ctacgacgag gaattcccga tcaagaagct cggcaccaac ctctgggcgg cgctggacta 4620 cacgctgttc ggcgaaggcc gtcccggcgt ggtgatcgga gccaaccagt ggctgttcag 4680 cgacgaggag ttcaagccca ccgccgccgc ctcgcagaac atcacggaca accaggcgct 4740 gatccagggc gtgcgtgaaa ccctggcacg gaacaacgtg caactggtga tggcgatcct 4800 gccggccaag gcacgcctgt acccggagaa cttcggcgag cagcagcccg cgtccctgca 4860 cgagcagctc taccagaact tccgccggat cgtggccgat gccggcatcc aggcacccga 4920 cctgctcggc ccgctgcagc aggccaaggc cgggggccag gtgttcctgc gcaccgacac 4980 ccactggacg ccctacggcg cccaggtggt cgccggccag ttggccacga cgatcaagcc 5040 gatcggcgtg ctcccggaaa gcggcaacgt ctacgtcacc gaaaccctgc cgggcgggcc 5100 gcacaagggc gacctgacca acttcctgcc gctcgacccg ctgttcgagg aactgctgcc 5160 gccgccggat caactggcca agcacaacac ccgccagcag gaggaaagcg cccccgccgg 5220 ggacgacctg ttcgccgaaa cccaggtgcc ggtggccctg gtcggcacca gctacagcgc 5280 cgacgaacgc tggaacttcg ccggcgcgct gcgccaggcg ctcggcagcg acctggtgaa 5340 cttcgccgag gacggccgcg ggccgctcct gccgatgctc aagttcctgc agagcgagga 5400 cttcaagaaa tccccgccgc gcctggtgat ctgggaattc cccgagcgtt acctgccgat 5460 ggcctatgac ctgagcgagt tcgacgccga ctggatcgcc cagctcaagg ccgccggccg 5520 ccaggacaag cagttggccg acaacaccgc taccaaccag ggcgcacgcc actgaacgcc 5580 cttccatcga t 5591 // |
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