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EBI DbfetchID AF023920; SV 1; linear; genomic DNA; STD; FUN; 4844 BP. XX AC AF023920; XX DT 01-NOV-1997 (Rel. 53, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Kluyveromyces lactis pyruvate dehydrogenase complex E1-alpha subunit (PDA1) DE gene, complete cds. XX KW . XX OS Kluyveromyces lactis OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces. XX RN [1] RP 1-4844 RA Zeeman A.M., Luttik M.A.H., Thiele C., van Dijken J.P., Pronk J.T., RA Steensma H.Y.; RT "Inactivation of the Kluyveromyces lactis KIPDA1 gene leads to loss of RT pyruvate dehydrogenase activity, impairs growth on glucose and triggers RT aerobic alcoholic fermentation"; RL Microbiology (Reading, Engl.) 144:3437-3446(1998). XX RN [2] RP 1-4844 RA Zeeman A.M., Luttik M.A.H., Thiele C., Pronk J.T., van Dijken J.P., RA Steensma H.Y.; RT ; RL Submitted (10-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL Section 2G, Institute for Molecular Plant Sciences, Leiden University, RL Wassenaarseweg 64, Leiden 2333 AL, The Netherlands XX FH Key Location/Qualifiers FH FT source 1..4844 FT /organism="Kluyveromyces lactis" FT /chromosome="V" FT /strain="JBD100" FT /mol_type="genomic DNA" FT /db_xref="taxon:28985" FT CDS complement(<1..970) FT /codon_start=1 FT /product="unknown" FT /note="similar to S. cerevisiae YD8557.10c, encoded by FT GenBank Accession Number Z47746" FT /db_xref="GOA:Q6CKB6" FT /db_xref="InterPro:IPR000994" FT /db_xref="UniProtKB/Swiss-Prot:Q6CKB6" FT /protein_id="AAD03772.1" FT /translation="MALQISKEDTQVLLKDKNVLIEPTIEKYRTAGQIAQTALNFLISL FT INKMYHEKSMEPLPIHQLCLLTDSFIADCLSTKFNNKVNEKGISMPTCIDIDEINENWC FT PEVDDIENLSRWNQKSRSTKSIELCNGVTSSLSGFLKVGDVAKITLGCHIDGYTCNVSH FT TVVIYPTVQDAQSGKLLPEAALLGGKADALAATHIAVESVTNLLAAANDLTKLPADFNA FT TKVTGSLIRTVVDTIAQSYNCCVVPGSRIRRIKRFLSGQNENIIVERDFKGVIWTESHQ FT EAAMLARTVNETAVDVKRKNPFITEDTVVPTDDFTVVKGEVY" FT CDS 1977..3203 FT /codon_start=1 FT /gene="PDA1" FT /product="pyruvate dehydrogenase complex E1-alpha subunit" FT /db_xref="GOA:O13366" FT /db_xref="HSSP:1NI4" FT /db_xref="InterPro:IPR017597" FT /db_xref="UniProtKB/Swiss-Prot:O13366" FT /protein_id="AAD03773.1" FT /translation="MLSLKAQSSVVGKSSSLRLVRNFSKNVRALSQVADETKPGDDDLV FT QIDLPETSFEGYLLDVPELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCH FT SSVGQEAIAVGIENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGG FT SMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNM FT AKLWNLPAVFCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVTKLKDWT FT VSGNGPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATE FT DEIKAYDKAARKYVDEQVELADAAPAPEAKMSILFEDVYVPGSETPTLRGRLQEDTWDF FT AKKSFAFRD" XX SQ Sequence 4844 BP; 1407 A; 1012 C; 891 G; 1534 T; 0 other; tgtaaacttc acccttgaca acggtaaaat catctgttgg gaccacggta tcttcggtga 60 taaatgggtt cttacgcttg acatcaacag cagtttcatt gacggttcta gcaagcatag 120 cagcttcctg atgagactca gtccaaatga cacctttgaa gtctctttcg acaatgatat 180 tttcgttttg accagaaagg aatcttttga ttcttctgat acgagaacca ggcacaacgc 240 aacaattata agattgagca atggtgtcaa ctacggtcct aatcaaggaa ccagtgacct 300 tcgtagcgtt gaaatcagct ggaagtttgg tcaaatcgtt tgcagcagct aataagttag 360 tgacggactc aacagcgata tgggtggcag ctagagcatc agctttacca cctagcaaag 420 cagcttcagg tagcaatttt ccagattggg catcctgaac ggttgggtaa atgaccacgg 480 tgtgggacac gttacaagtg tacccatcga tgtgacaacc caaagtgatc ttagcgacat 540 caccaacttt caaaaaccca gacaaactag aagtaacacc gttacatagc tcgatcgatt 600 tggtagatct agatttttgg ttccaacggg acaagttttc gatgtcatcc acttcaggac 660 accagttctc gtttatttca tcgatatcaa tacaagttgg cattgagatt cccttctcgt 720 taaccttatt gttgaactta gtacttaaac aatcggctat gaacgaatca gtcaataaac 780 aaagttgatg aatcggtagc ggctccatag acttctcatg atacatctta ttgatcaaac 840 tgatcaaaaa gtttaaagca gtctgagcaa tttgacctgc agttctatat ttctcaattg 900 ttggttcgat caatacattc ttatctttca ataacacctg agtgtcttcc tttgagattt 960 gtaacgccat agtacggata attttatcca caaattatta gtattagctg ttttctgata 1020 caaaggaggg aacgatacac tatgataatg atgtaagtat gtacctcgtt tctctttacc 1080 tcatctcatc tcatcgcttt aaagttttca ctgatacttt ttttttccaa atttctttcc 1140 tggatacaac ctttcctgcc acacagcact aagatatttc aaaaattttc caaaatatta 1200 cccggacctc gatcagaaga gaatgaaaaa gaaatcactt tctgatcacg tgcgccaaaa 1260 gtattaatat tcgggtaatg tgtatcatta gaactcatga cccgcatatt atccgggtaa 1320 tagatcggct gcccatgttc cacccaaaca ccgcgctgtg cacgtcactt ttcccactct 1380 ttctaacaac aatcccgtct gtctaattga atcagcacaa taaactctac ctacaccaga 1440 cgaaactgtc agatatccca taataagtag aatatcaata ctctccgcat catcagctac 1500 ccaacggttc aagtttgacc ccaattagag caaaattgac cttgcttata cccacttctt 1560 gttatttcaa gtcccttatc tataccgact ggctggctac gaaattttgt gaagtttttt 1620 tcccaagttc acaagtattt aaaggcggat ttaattgtga atatgatgag aaacagttgt 1680 tgcgtgatta agaacctttt tttggtcttt cagtgatact gagccaagca attgattaat 1740 tgactgactg attgattgat tgattgattt ttgtcaaatt ttttggaaca aggaatcgtt 1800 cattagttgg cttttttttg ttttaatata gaatcaggaa ctcagagctt cttcttcttt 1860 atttcttctc tatacaacat attttgccat cactactgct gagtgtgtat ttgtgtgttc 1920 taattgttgg tcgctatagt agttaatacc gaaaagaaca ataaattagg caaaccatgc 1980 tatctttgaa agctcaatcc tctgtggttg ggaagtccag ctctttgaga ttggttagaa 2040 acttttctaa aaacgtccgt gctttgtccc aggttgctga tgaaactaag ccaggtgatg 2100 atgacctagt tcaaattgat ttgccagaaa cctcttttga aggttatctt ttggatgttc 2160 ctgaattaag ttatcaaacc accaagtcca atttgctaca aatgtacaag gatatgatta 2220 tcgttagaag aatggaaatg gcctgtgacg ctttgtacaa ggctaagaaa attagaggtt 2280 tctgtcactc ctctgtcggt caagaagcca ttgccgttgg tattgaaaac gctatcacta 2340 agcgtgatac cgtcatcacc tcttacagat gtcatggttt cacctacatg agaggtgctg 2400 ctgttcaagc tgtgttggct gaattgatgg gtagaagaac tggtgtgtcc ttcggtaagg 2460 gtggttccat gcacttgtac gcccctggtt tctacggtgg taatggtatc gttggtgccc 2520 aagtcccatt gggtgctggt ttggccttcg ctcatcaata caaacacgaa gatgcttgtt 2580 cttttgcctt gtacggtgat ggtgcctcta accaaggtca agttttcgaa tccttcaaca 2640 tggccaagtt atggaactta ccagccgtct tctgttgtga aaacaacaag tacggtatgg 2700 gtaccgctgc cgcaagatct tcagccatga ctgaatactt caagcgtggt caatacattc 2760 ctggtttgaa ggttaacggt atggatatct tggctgttac caagcttaag gactggactg 2820 tctccggtaa cggtccaatc gttcttgaat acgaaactta cagatatggt ggtcactcta 2880 tgtctgatcc aggtactact tacagaacca gagatgaaat ccaacacatg agatctaaga 2940 acgatccaat tgcaggttta aagatgcact tattggaatt gggtatcgcc acggaagatg 3000 aaattaaggc ttacgacaag gctgctagaa agtacgtcga tgagcaagtc gaattagctg 3060 atgctgcccc agctccagaa gctaagatgt ccatcttgtt cgaggatgtc tacgttccag 3120 gttctgaaac tccaacccta agaggtagat tgcaagaaga tacttgggat tttgctaaga 3180 agagctttgc tttcagagat tagagcaaga acgaattagg taattgaagt taaagttaac 3240 ttcttttgcg tttatccttt tttttattcc catctctcta ctcttccctt ttcgaaagac 3300 aactatattc ctataaactg ccctttcaca atattatgct ataaggttca caagatgata 3360 ctatttgtat agtgcatctg ctattttcca ccaatcaata ttaaagtacc aaaatcataa 3420 aaaaacacct tttagataca agtgtttttt attcatttca ttcacggtat ggtttctatt 3480 cagataactg tttttttcgg ttttaaaaca tgaatgttca tctgtgactt ctcattctct 3540 ctctctccat gagacttcgt tgatgcttat tctcaatgcc gagaacatta tctggttact 3600 gatgcctgcc attacggatt acctgcgttt taatttcttc ccaaacaaaa caacgataca 3660 gaattttctc attactccca aaaactacct caataattcg tgctacaatc ttcttttctt 3720 acgttgatct tgcgcctgtt ataacatgat ttattgtgaa ctaggttttg taactaatct 3780 gacattgttc ttcaccataa attgtctctc cctttttggt tgctacgata caatgtcgtc 3840 aaatgcgaag cgcatattag acagatgaaa agttacaata ccttgtccat cattgaatca 3900 tctactcacc tttctaatga ctgatctcac gacgacttac cccaataatc atctcatctc 3960 tatttactag acttacatct agtctatata taaattgtat ttactgaatt aaagtatcat 4020 agatatacta gagacagttt tgtcaccctg gttccagagt cactgcagga ccctactcat 4080 cgacttccta aatgtacact ttgtcctgat ttcatcacta tctctgtttt catttaaggg 4140 aagaaattat tcattactta agaccgttga tgtttaaatc tgaaacaacc tcaaaatggc 4200 atcatgatga aaggggaaat atagtgattg cagattccgg gagatttgag attcttttag 4260 tgaattctcc aaagtagcta ccatgatatc gttacttatg tgctaatcta tcaattgaat 4320 aactgaacaa ttattgttca aagtattaag aatacatcca aaagtcttca aactttaagg 4380 aatttaagag taacactacg gctgactaga gcatattaac tattactttg ctagaaatgt 4440 ctagaggtat acatcatcaa tgaatctcag gatatcttat ttcaattgac taatgtggga 4500 tcctccccgg tgcctaaact ggatacagga tccacttagg gtccaaatac cctcactcgc 4560 caagagagag tggacagagc tttttttcta agaagagagt gaagacgcct tcagttggta 4620 ctccatcgac tgcatcatac catgatatac tcttcctctc tatgatagtg tctcccataa 4680 gaccagtgtc aaacagcatc ctaagcacct cctggtagat ccgatcatgc cgcctccacc 4740 cataatgctc tctcccattg gacatgagac cttaggagag aagccactcg aaaaaatggt 4800 aataagtctt ggaaaaatga gagatgttgc atatgcggag atct 4844 // |
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