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EBI DbfetchID AF022812; SV 1; linear; genomic DNA; STD; PRO; 6019 BP. XX AC AF022812; XX DT 06-APR-1998 (Rel. 55, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Dehalospirillum multivorans GTP cyclohydrolase II (ribA) gene, partial cds; DE and 3,4-dihydroxy-2-butanone-4-phosphate synthase (ribB), riboflavin DE synthase (ribH), tetrachloroethene reductive dehalogenase catalytically DE active subunit (pceA), and tetrachloroethene reductive dehalogenase DE membrane-bound subunit (pceB) genes, complete cds. XX KW . XX OS Sulfurospirillum multivorans OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales; OC Campylobacteraceae; Sulfurospirillum. XX RN [1] RP 1-6019 RX PUBMED; 9696761. RA Neumann A., Wohlfarth G., Diekert G.; RT "Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, RT sequencing of the encoding genes, and expression of the pceA gene in RT Escherichia coli"; RL J. Bacteriol. 180(16):4140-4145(1998). XX RN [2] RP 1-6019 RA Neumann A., Wohlfarth G., Diekert G.; RT ; RL Submitted (04-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Stuttgart, Allmandring 31, Stuttgart D-70569, RL Germany XX FH Key Location/Qualifiers FH FT source 1..6019 FT /organism="Sulfurospirillum multivorans" FT /mol_type="genomic DNA" FT /db_xref="taxon:66821" FT CDS <1..426 FT /codon_start=1 FT /transl_table=11 FT /gene="ribA" FT /product="GTP cyclohydrolase II" FT /EC_number="3.5.4.25" FT /db_xref="GOA:O68248" FT /db_xref="InterPro:IPR000926" FT /db_xref="UniProtKB/Swiss-Prot:O68248" FT /protein_id="AAC60784.1" FT /translation="HSECLTGDALGSLKCDCGEQLEFALQNISLLGGMIIYLRQEGRNI FT GLFNKVNAYALQDQGFDTIEANHQLGFKSDERSYEVVETILEHFKIDKIRLLTNNPKKM FT SCLKNIMIIERWPIIIPSNNHNVDYLKTKKEMMGHLL" FT CDS 436..1506 FT /codon_start=1 FT /transl_table=11 FT /gene="ribB" FT /product="3,4-dihydroxy-2-butanone-4-phosphate synthase" FT /db_xref="GOA:O68249" FT /db_xref="HSSP:1G58" FT /db_xref="InterPro:IPR016299" FT /db_xref="UniProtKB/Swiss-Prot:O68249" FT /protein_id="AAC60785.1" FT /translation="MNAILSDQKTFQAIIRVNQAIEDIRQGKMVVMVDDEDRENEGDLV FT YAASFSTPQKVNFMASHAKGLICVAISKKIANRLQLEPMVKKNDSSYETAFTITVDART FT AATGISAGERDMTIKILADGGSHESELVRPGHIFPLIAKEGGALVRIGHTEGSVDLCRL FT AGQGDSAVICEIMKEDGTMARRPDLDIFCAKHELNIVYISDIVEYRMMNESLIRVIAES FT TTQFLGKDARRYDFVDHNDNHHIAYAFGNIKNRSAVKFHSIMPDNELLADTKKYNSLIQ FT AIHYLQKSGGVLIFMDNGTQDMSKIREYGIGAQIIKHLGIENIELLSDSKNKEFVGISG FT FGLSVIKSTNVNETVA" FT CDS 1715..2185 FT /codon_start=1 FT /transl_table=11 FT /gene="ribH" FT /product="riboflavin synthase" FT /EC_number="2.5.1.9" FT /db_xref="GOA:O68250" FT /db_xref="HSSP:1HQK" FT /db_xref="InterPro:IPR002180" FT /db_xref="UniProtKB/Swiss-Prot:O68250" FT /protein_id="AAC60786.1" FT /translation="MNIVEGKLSLNGDEKVAIINARFNHIITDRLVEGARDAYLRHGGK FT DENLDLVLVPGAFEIPMALNRLLACSKYDAVCCLGAVIRGSTPHFDYVSAEVTKGVANV FT ALQFAKPVAFGVLTVDSIEQAIERAGSKAGNKGFEAMVTVIELLSLYSALKN" FT CDS 2380..2913 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /note="orf1" FT /db_xref="InterPro:IPR004675" FT /db_xref="UniProtKB/TrEMBL:O68251" FT /protein_id="AAC60787.1" FT /translation="MAHIALCVYENMSDSVKKHADKKLEKTGSLGEIFQLLALNEDVYF FT ATDTMVSKFLLKETLLPYVTKQRIAILVSLDNGCKMCAGVHKQLARALGVSEVEIEEIA FT LGIDALSCSEAEKRLLHFCIRASKKDNYKIMKEDIEVIKAFGYSEKEIFEAVCIVGYFN FT YINTLSNTFGLGSE" FT CDS 3272..4777 FT /codon_start=1 FT /transl_table=11 FT /gene="pceA" FT /product="tetrachloroethene reductive dehalogenase FT catalytically active subunit" FT /note="PCE dehalogenase" FT /db_xref="GOA:O68252" FT /db_xref="InterPro:IPR006311" FT /db_xref="UniProtKB/TrEMBL:O68252" FT /protein_id="AAC60788.1" FT /translation="MEKKKKPELSRRDFGKLIIGGGAAATIAPFGVPGANAAEKEKNAA FT EIRQQFAMTAGSPIIVNDKLERYAEVRTAFTHPTSFFKPNYKGEVKPWFLSAYDEKVRQ FT IENGENGPKMKAKNVGEARAGRALEAAGWTLDINYGNIYPNRFFMLWSGETMTNTQLWA FT PVGLDRRPPDTTDPVELTNYVKFAARMAGADLVGVARLNRNWVYSEAVTIPADVPYEQS FT LHKEIEKPIVFKDVPLPIETDDELIIPNTCENVIVAGIAMNREMMQTAPNSMACATTAF FT CYSRMCMFDMWLCQFIRYMGYYAIPSCNGVGQSVAFAVEAGLGQASRMGACITPEFGPN FT VRLTKVFTNMPLVPDKPIDFGVTEFCETCKKCARECPSKAITEGPRTFEGRSIHNQSGK FT LQWQNDYNKCLGYWPESGGYCGVCVAVCPFTKGNIWIHDGVEWLIDNTRFLDPLMLGMD FT DALGYGAKRNITEVWDGKINTYGLDADHFRDTVSFRKDRVKKS" FT CDS 4774..4998 FT /codon_start=1 FT /transl_table=11 FT /gene="pceB" FT /product="tetrachloroethene reductive dehalogenase FT membrane-bound subunit" FT /note="PCE dehalogenase" FT /db_xref="UniProtKB/TrEMBL:O68253" FT /protein_id="AAC60789.1" FT /translation="MKLLNILNYKALGLSLLYLALILVTFQISMGSYGEDEPQAGSILM FT VAGLIFSIIGVFLLMKFKPTYILWEKLKR" XX SQ Sequence 6019 BP; 2039 A; 839 C; 1189 G; 1952 T; 0 other; catagtgaat gtttaacagg ggatgcactt ggctctttaa aatgcgattg tggtgaacag 60 cttgagtttg cattgcagaa tatctcatta cttggtggaa tgatcattta cttacgtcaa 120 gaaggacgca atattggttt gtttaataaa gtaaatgcct atgcgctaca agatcaaggg 180 ttcgatacta ttgaagcaaa tcatcagttg ggttttaaaa gtgatgaacg tagttatgaa 240 gtggtagaaa ctattttaga gcattttaaa attgataaaa ttcgtttgct tacgaataac 300 cctaaaaaaa tgagttgttt aaaaaacatt atgattattg aaagatggcc aattattata 360 ccttcaaata atcataacgt tgattatttg aaaactaaaa aagaaatgat gggtcattta 420 ctttaaagga aaaacatgaa tgcaatatta agcgatcaaa aaacattcca ggctattatc 480 cgcgttaatc aagcgattga ggatattaga caaggcaaga tggttgttat ggtagatgat 540 gaagatagag aaaatgaagg tgatttagtt tatgctgctt ctttttctac accacaaaaa 600 gttaatttta tggcaagtca tgcaaaaggg cttatctgtg ttgctatttc aaaaaagata 660 gcaaatagat tgcaattaga gcctatggtc aaaaaaaatg attcatcata tgaaaccgct 720 tttacaatta cagtcgatgc aagaacagca gcaacgggta tatctgcagg tgagcgtgat 780 atgaccataa aaattttagc agatggtgga tcgcatgaat cagaactcgt tcgtcccggc 840 catatttttc cattgattgc taaagagggt ggtgcattag taagaatagg tcatactgag 900 ggttctgtag atttatgtcg tttagcagga cagggtgact ctgccgttat ttgtgaaatc 960 atgaaagaag atggaacaat ggctagacgt cctgatctgg atatcttttg tgcgaagcat 1020 gaattaaata ttgtctatat ttctgatatt gtagagtatc gcatgatgaa tgaatcactt 1080 attcgtgtta ttgccgaatc aacaacgcaa tttttaggca aagatgccag acgttatgac 1140 tttgtggatc ataatgataa ccatcatata gcctatgcgt ttggaaatat taaaaataga 1200 tcagcagtaa aatttcatag tattatgcct gataatgaac ttttagctga tacaaaaaaa 1260 tataatagct taattcaagc catacactat ctgcaaaaaa gtggtggagt attgattttt 1320 atggataatg gaacacaaga tatgtctaaa attcgtgaat acggtatagg tgcgcaaata 1380 attaaacatc taggtataga aaatatagaa cttttaagtg atagtaagaa taaagagttt 1440 gttggcatta gtgggtttgg ccttagcgtc attaaaagta cgaatgttaa tgaaactgtt 1500 gcttaaaata acaagtattt attgtcaatg aaaataaaga gtaaaaaagg tatcagtgta 1560 atgggtttct atcttgtagc gtatggttta agttgtgttg tgttgggaat gatgattggc 1620 acatgcattc aattctctaa aattaataaa tgagtctgca atggattata tcagtatatc 1680 cattttgtta aaatgtatga tatgaaggat aataatgaat attgtagaag gtaaattgtc 1740 cctaaatggt gacgaaaaag ttgctattat taatgcacgc tttaaccata tcatcacaga 1800 tcgtttggta gaaggagcgc gggatgccta tttgagacat ggtggcaaag atgaaaattt 1860 agatcttgtt ttggtaccgg gtgcatttga aattccaatg gcacttaatc gtcttttggc 1920 ttgtagtaaa tacgatgcag tatgttgttt aggtgcagta atacgtggtt caacgccaca 1980 ttttgactat gtgtctgcag aagtgaccaa aggtgtggcc aatgtagcac ttcaatttgc 2040 taaaccagtc gcttttggtg tcctgacagt ggatagcatt gaacaagcaa ttgaacgagc 2100 aggtagtaaa gcaggtaata aaggttttga agcaatggtt actgtcattg aactattaag 2160 tctatactct gcattaaaga attaagaaaa agttatagat gtagatattg atagaattga 2220 tggtgttttt ttgagcatcg agatagatag aaatactact gaatattatt taatgattaa 2280 agaattagta gaaaaattat tattatctac aaacaaccta taattatttt ctatacttac 2340 atttataata attaaatatt ttaaaaataa ggatagaata tggcacacat agcactttgc 2400 gtctatgaaa atatgagtga ttctgttaaa aaacatgcgg ataagaaatt ggaaaaaacg 2460 ggtagcttgg gtgagatatt tcagctactt gcattaaatg aagacgttta ttttgctaca 2520 gatactatgg tttccaagtt tctcttgaaa gaaacattac tcccttatgt caccaaacaa 2580 agaattgcaa tattagtatc attggataat gggtgtaaaa tgtgtgcagg tgtacacaag 2640 caacttgcac gggctttagg tgtgagtgag gttgaaattg aagaaatagc attaggtatt 2700 gatgccctaa gttgtagtga agctgaaaaa aggttacttc atttttgtat tcgtgcctca 2760 aaaaaagata actataaaat catgaaagaa gatatagaag ttatcaaggc gtttggatac 2820 agtgaaaaag agatctttga agcggtttgt attgttggat attttaatta tatcaatacc 2880 ttatctaata catttggtct tggaagcgaa taaaacataa aatattaggg acgacttttt 2940 atcttacttt ttttttaaaa gcgttccttt aacacaaatt attgtctgat atattgacaa 3000 aatctttata aaatctccaa aaatataaaa aaagaaaagt atttacttat tacttcgtaa 3060 atattattaa ctttcattat tttaaaatta aaatctatat ctttcctata acaaagctat 3120 aacttttgta tatcattctt aaaatgtgaa attaattcat aaacacatta aaaaataaat 3180 aactgtactt ggggttattt ataaaaggtt acaagaaggt ttgaatacca ctgtgtaagt 3240 tgttactctt ggtatttttt aaggaggaag tatggaaaag aaaaaaaagc ctgaactctc 3300 aagaagagat tttggtaagt tgattatcgg aggtggagct gcagcaacga tagctccatt 3360 tggtgtacca ggtgcaaatg cagctgaaaa agagaaaaat gcagctgaaa ttcgtcaaca 3420 atttgcaatg actgcaggtt ctcccatcat agttaacgac aaattggaaa gatatgctga 3480 agtacgaaca gcgtttactc atccaacctc attttttaaa ccaaactaca aaggtgaagt 3540 gaaaccttgg tttttatcag catatgatga gaaagtacgt cagattgaaa atggtgaaaa 3600 tggtcctaag atgaaagcta aaaatgtagg agaagctaga gcaggtcgtg cacttgaagc 3660 tgctggatgg accttagata ttaactatgg aaacatttat ccaaatagat tttttatgtt 3720 atggtctggt gagactatga ctaacaccca attgtgggca ccagtagggt tagatagaag 3780 acctccagat acaactgatc ctgtggaact tacaaattat gtaaaatttg ctgcacgtat 3840 ggcaggtgct gatttggtag gtgttgcaag attaaatcgt aactgggttt attctgaggc 3900 agtaactata ccagctgacg taccttatga acaatctttg cataaagaga ttgaaaagcc 3960 aatcgttttc aaagatgttc cactaccaat agaaactgat gatgaattaa ttatccctaa 4020 tacttgtgaa aatgttatcg ttgcaggtat cgctatgaac cgcgaaatga tgcaaacagc 4080 tcctaacagt atggcatgtg caacaacagc attttgttat tcacgtatgt gtatgttcga 4140 tatgtggtta tgccagttta ttcgttatat gggttactat gcaataccaa gctgtaatgg 4200 tgttggacaa tcagttgcct ttgctgttga agcaggttta ggacaagcta gtcgtatggg 4260 tgcttgtatt actcctgaat ttggaccaaa cgtaagactt acaaaagtct ttacaaatat 4320 gcctttagtt ccagataagc ctatcgactt tggagtaaca gaattttgtg aaacatgtaa 4380 aaaatgtgca cgtgagtgtc cttcaaaagc aattactgaa ggtccaagaa cttttgaagg 4440 acgaagtatt cataatcaat caggtaaatt acagtggcaa aatgactata ataaatgctt 4500 aggttattgg ccggaatctg gtggatattg tggcgtatgt gtagctgttt gcccctttac 4560 aaaaggcaat atttggattc atgatggcgt tgaatggctt attgataaca caagattctt 4620 agacccatta atgcttggta tggatgatgc attgggctat ggtgcaaaac gaaatattac 4680 agaagtttgg gatggaaaga ttaatacata tggtctagac gcagaccatt ttagagacac 4740 tgtaagcttt agaaaggata gggttaaaaa atcatgaaat tattaaatat tttaaattat 4800 aaagcgcttg gtttgagttt actttatttg gcattgattt tggttacttt ccaaatttca 4860 atgggaagtt atggagagga tgagccacaa gctggtagta tattaatggt tgctggattg 4920 attttttcga tcatcggcgt ttttctttta atgaagttca agccaacata tattttatgg 4980 gaaaagctta agcgttaatt tctaaatcta tgcataaagt agggtttaca tcattacatg 5040 taaaccctac ttttctcttt ttctaagtca taaatatgct taaatataca gcacgtttag 5100 cttgacattc ccattatttt ggagtgactc caaagcgctg atgaagtggc tgacttgatt 5160 atgacattta tgcagggatt acgagttcaa tcacgaaatc aatactctgg tgatgcaatc 5220 aagcgagcca taaaaacatt tttggctttg ataaagtcaa tggaaaagaa aaaaagtata 5280 tttttaggtt tgtaattgct ataagaaaac gttataactt ttttttcagt tttaagagag 5340 atgacttggt aaataactct cttttgagct agatcgccaa ttatctctct tgttttaaaa 5400 agttagagtc gtaatgcgct cttctttctc tttttcataa taagcttcta cgcactcttt 5460 atacgctgtt aaaaataaca tgctgcatcg tgattcttta gaatcgtcat ctttgatttg 5520 tttcaaaata ttaccagcta aaatgtaagt ctctgaaagc gtagcattaa aagcagaatc 5580 taaataaagc gaagcttgtg caagcagtga tgggcaacct ttgatttcaa aaccacatct 5640 gacagtctta tcatcttcaa tttgcatagt aaggacaact ttaccttttc catcggggtg 5700 aaggcatgtc gctttggcat tgtaattatc taatagtcca taaaaacgag ggtttttcat 5760 atggtcttct aattggctta tgagccctgt gtagtcaaaa tcatcacgca tagtgtttat 5820 tcctttagtt ataattttaa ttatggcaaa aaagtatagg acgtttatag gtaaaatata 5880 agttaattag acgaaaaaac ctattatctc atactgcgca ttcctaatgt ctagaagtat 5940 tttcatacaa atatagcata ttaattaaca tggctaagat caatgataaa ttttgcgcct 6000 tttttagaat tcctgcagc 6019 // |
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