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EBI DbfetchID AF019038; SV 1; linear; genomic DNA; STD; PRO; 3167 BP. XX AC AF019038; XX DT 01-OCT-1997 (Rel. 52, Created) DT 10-MAY-2007 (Rel. 91, Last updated, Version 5) XX DE Pseudomonas knackmussii dienelactone hydrolase (clcD) and maleylacetate DE reductase (clcE) genes, complete cds; and unknown gene. XX KW . XX OS Pseudomonas knackmussii OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-3167 RX PUBMED; 9171435. RA Kasberg T., Seibert V., Schlomann M., Reineke W.; RT "Cloning, characterization, and sequence analysis of the clcE gene encoding RT the maleylacetate reductase of Pseudomonas sp. strain B13"; RL J. Bacteriol. 179(11):3801-3803(1997). XX RN [2] RP 1-3167 RA Kasberg T., Seibert V., Schloemann M., Reineke W.; RT ; RL Submitted (12-AUG-1997) to the EMBL/GenBank/DDBJ databases. RL Institute for Microbiology, University of Stuttgart, Allmandring 31, RL Stuttgart 70569, Germany XX FH Key Location/Qualifiers FH FT source 1..3167 FT /organism="Pseudomonas knackmussii" FT /strain="B13" FT /mol_type="genomic DNA" FT /db_xref="taxon:65741" FT CDS <1..512 FT /codon_start=3 FT /transl_table=11 FT /product="unknown" FT /note="similar to product encoded by GenBank Accession FT Number M16964" FT /db_xref="GOA:P0A178" FT /db_xref="InterPro:IPR005064" FT /db_xref="UniProtKB/Swiss-Prot:P0A178" FT /protein_id="AAB71538.1" FT /translation="AGMGTSIHLAAALLAAQAGVDLLHVPYKGSTPAAADLIAGRLSMM FT VDSITAQQSFIKSGRVRALGVTSLQPAPSLPGIPPLAQAADQPNFEVLTWFGLFVPSRT FT SPDIVKVLNTAMNEALKTPEVQKALADIGASAQGGTSQALGVLWDNEIDRWGQLIVHHR FT LNTNEL" FT misc_feature <1..512 FT /note="ORF1" FT gene 528..1238 FT /gene="clcD" FT CDS 528..1238 FT /codon_start=1 FT /transl_table=11 FT /gene="clcD" FT /product="dienelactone hydrolase" FT /EC_number="3.1.1.45" FT /note="similar to product encoded by GenBank Accession FT Number M16964" FT /db_xref="GOA:P0A115" FT /db_xref="InterPro:IPR002925" FT /db_xref="PDB:1DIN" FT /db_xref="UniProtKB/Swiss-Prot:P0A115" FT /protein_id="AAB71539.1" FT /translation="MLTEGISIQSYDGHTFGALVGSPAKAPAPVIVIAQEIFGVNAFMR FT ETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQREQAYKLWQAFDMEAGVGDLEAAI FT RYARHQPYSNGKVGLVGYCLGGALAFLVAAKGYVDRAVGYYGVGLEKQLKKVPEVKHPA FT LFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFARTSSSGYVASAAALANE FT RRLDFLAPLQSKKP" FT gene 1235..2293 FT /gene="clcE" FT CDS 1235..2293 FT /codon_start=1 FT /transl_table=11 FT /gene="clcE" FT /product="maleylacetate reductase" FT /EC_number="1.3.1.32" FT /db_xref="GOA:O30847" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/Swiss-Prot:O30847" FT /protein_id="AAB71540.1" FT /translation="MNFIHDYRSPRVIFGPDSLARLPQELERLGIDRALVLTTPEQAPL FT GRQVAEPVIGHVAAFYDGATMHVPALVAEEACKIARTSEANGVIAIGGGSTIGLAKIVA FT LRTELPIVAVPTTYAGSEMTSIFGITEGGVKKTGRDARVMPRAVIYEPRLTLELPLSIS FT VTSAINAIAHAVEGLYAPDATPLLTIMAQEGIAATVRAISRMYQSPRDLQARGDALYGA FT WLCASVLGNVSMALHHKLCHTLGGTLDLPHAQTHTVVLPHALAYNARAVPDAMRVLRIA FT LGHDDPPTALYELARDNGAPVALRDLGMREEDIEHVGDLALQDRYPNPRELDRDALLAL FT LRDAYHGRPPSA" XX SQ Sequence 3167 BP; 620 A; 938 C; 993 G; 616 T; 0 other; ctgcaggcat ggggacgtcg atccatctgg cggccgctct gctggccgct caggccgggg 60 ttgacctgct ccacgtgccc tacaaaggga gcacgccagc tgccgccgac ctgatcgcgg 120 ggcgactctc catgatggtc gattcgatca ccgcacagca gtcgtttatc aaatctggac 180 gggtgcgtgc gctgggtgtc accagcttgc aacccgcgcc ctcattgcct gggattcctc 240 ccctggcgca agcagccgac caaccgaact tcgaggtcct gacatggttt ggcttatttg 300 tgccgtcacg tacctcgccg gacatcgtca aggtgctcaa cacagcaatg aatgaagcac 360 tgaagacccc ggaggtacaa aaggcgctgg cggatatcgg cgcttcagcg caggggggaa 420 catcgcaagc tctgggcgtc ttatgggaca acgagatcga ccgatggggc cagttgatcg 480 tccaccatcg actcaacacc aatgaactgt aatcggagag atggaagatg ttgactgaag 540 ggatatcgat tcaatcgtat gacgggcata cattcggcgc gctcgtgggc tcgccggcca 600 aagcgcccgc tcccgtgatt gtgatcgctc aagaaatatt tggtgtgaac gcgttcatgc 660 gagaaacggt gtcatggctg gtcgaccagg ggtatgcggc agtttgccct gatctgtacg 720 cgcgccaggc gccaggtaca gcactcgatc cgcaggatga gcgccagaga gagcaagcct 780 acaagctctg gcaggccttc gacatggagg ccggcgtggg cgatctggag gctgctatcc 840 gctatgcgcg acaccaaccc tacagcaacg gcaaggtggg attggtgggg tattgcctgg 900 gcggtgcgct tgcctttcta gtggccgcca aaggatacgt ggatcgcgcc gtaggctact 960 acggtgttgg actggagaag cagctcaaga aggtcccgga agtcaagcat ccggcgttgt 1020 ttcacatggg cggccaagac cacttcgtgc ccgcgccaag ccgccagctg attactgaag 1080 gcttcggtgc caatccattg ctgcaagtgc actggtacga agaggccgga cactcgttcg 1140 ccaggacgag cagttcgggc tatgtggcga gtgccgcggc gttggccaac gaacgtagac 1200 tggatttcct ggcgcccttg cagagcaaga agccatgaat ttcattcacg actatcgttc 1260 cccgcgggtg atttttggac ccgacagcct cgcccgattg ccgcaggaac tggaacgtct 1320 cggcattgac cgggcgctcg tgttgaccac gcctgagcag gcgcccctgg gacggcaagt 1380 agccgagccg gtcatcggcc acgtggcagc cttctacgat ggtgcgacca tgcatgtacc 1440 cgctttggtg gctgaggagg cctgcaagat tgcgcgcacg agtgaggcta atggcgtcat 1500 tgcgattggt gggggatcta ctatcggact cgctaagatc gtggcgctcc gtacagagtt 1560 gcccatcgtc gctgtgccca cgacatatgc aggtagcgag atgacgtcca tcttcggtat 1620 cacagagggt ggcgtcaaga aaaccggccg cgacgcgcgt gtcatgcccc gtgcagttat 1680 ctacgagccc cggctgaccc tagagctacc ccttagcatc agcgtgacca gcgcgatcaa 1740 tgcaatagcc catgcagttg aaggcctgta tgcgccagat gccacgccgc tgcttaccat 1800 catggcccag gaaggaatcg cagcgacggt acgcgcgatt tccagaatgt atcaatcacc 1860 aagagatctt caggctcgcg gcgacgcgct ttatggcgct tggctctgcg cgagcgtgct 1920 tggcaacgtc agtatggcat tgcaccacaa gctgtgccac acgctgggag gaacgctcga 1980 tctccctcat gcccagaccc atacggtcgt gctgccccac gcgctggcct ataacgcgcg 2040 cgcggtgcca gacgcaatgc gtgtgctgcg cattgccctg gggcatgacg atccacccac 2100 ggccttgtac gaattggctc gcgacaatgg cgcacccgtg gccttgcgcg atctcggaat 2160 gcgcgaggag gatattgagc atgttggcga cctggcattg caggaccgct atcccaatcc 2220 gcgggaactg gatcgagacg cattgcttgc attgctgcgc gatgcgtacc acggacgccc 2280 gccttccgct tgaggaggtt ggcatggaat tttcttttga tacaagttga cgccccaggc 2340 gttggccact ccagccgcac agccgggcgg agtgaacaca acaacaccac caccaccacg 2400 aggttgcgat ggcgcatctg tcctactctc ctccgaggaa tttcgacgcc ctttaccgac 2460 aacgtgtatt ccagtcccat caggccgtgc gcatgcatga cgcggcagcg cgagagttgg 2520 ccgaccatag cctgacatgg cgttcgggca acgtcgatgc ggctttgtac aaggcacgcg 2580 cgcggcgcct aaccctgttc tcctttcgct atggtgcaga ggtcgaaatt gcaccaagac 2640 cgagcgagga gttcatcgtg gtgcatcatt cgatcagagg cgccgcagaa ttgagcgttg 2700 acggccagca tatggtattg ccccaagggc gcaccggctg gatggcccca cggcgaaatt 2760 ggcgcatgcg ctgggaggcg gggtgtgagc aactcatcct gaaaattccg cgcgacatga 2820 tcgaggcggg tggtatcgca ccgatggcac cacttgcgac tttgccagca gggctggata 2880 cgcagtggct gctgctggtc caggtgttgc tgggcgcgtc gtccgcaccg acctgcgcgg 2940 gagagcccgc cgcgtgggtg gagcatatcg agcagggctt ggcttcgctg ctgcgcgcgg 3000 ccagttcctg tagtttgccg cgcgagtcga gcgatcggaa cttcgttgcg cgcgacccca 3060 tcgcccggct tcaggccatg gaagactata tgcgtgagcg ccttggggcg cctatcggac 3120 tggcagacct atcaggtgcg gccggcgtca gcgcccgggt cctgcag 3167 // |
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