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EBI DbfetchID AF016588; SV 1; linear; genomic DNA; STD; PRO; 3457 BP. XX AC AF016588; XX DT 01-OCT-1997 (Rel. 52, Created) DT 05-JUL-1999 (Rel. 60, Last updated, Version 2) XX DE Pyrococcus furiosus amylopullulanase (apu) gene, complete cds. XX KW . XX OS Pyrococcus furiosus OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-3457 RX PUBMED; 9293009. RA Dong G., Vieille C., Zeikus J.G.; RT "Cloning, sequencing, and expression of the gene encoding amylopullulanase RT from Pyrococcus furiosus and biochemical characterization of the RT recombinant enzyme"; RL Appl. Environ. Microbiol. 63(9):3577-3584(1997). XX RN [2] RP 1-3457 RA Dong G., Vieille C., Zeikus J.G.; RT ; RL Submitted (30-JUL-1997) to the EMBL/GenBank/DDBJ databases. RL Biochemistry, Michigan State University, East Lansing, MI 48824, USA XX FH Key Location/Qualifiers FH FT source 1..3457 FT /organism="Pyrococcus furiosus" FT /strain="DSM 3638" FT /mol_type="genomic DNA" FT /db_xref="taxon:2261" FT CDS <1..798 FT /codon_start=1 FT /transl_table=11 FT /note="putative integral membrane component of starch FT degradation products transport; similar to Escherichia coli FT MalG" FT /db_xref="GOA:O30771" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/TrEMBL:O30771" FT /protein_id="AAB71228.1" FT /translation="IGLGVIKNVTLKSGEVNGLKISEGKLAGFIRIRDSGKAELTAIGK FT FVNSKFFHHLRNSLILATLTVFLTLLFVVPAAYAFSRLKFFGREHILYFYLMFTQVAGG FT LGIAGLIALYGMLVKLGLYNKLPALAIIYAAGSVPFNTWLLKSYIDSISPDFDEAALVD FT GASYLQIIRYVLLPMALPGIATVAVMSFIGGWTEFILASLLLTEENQPLSVWIYTLMGG FT IGRGIDWNYFAAAALLFALPVFIMFMLAQNYIRSGLTVGGLKE" FT gene 809..3370 FT /gene="apu" FT CDS 809..3370 FT /codon_start=1 FT /transl_table=11 FT /gene="apu" FT /product="amylopullulanase" FT /function="hydrolyzes alpha-1,6 and alpha-1,4 linkages in FT starch-related polysaccharides" FT /db_xref="GOA:O30772" FT /db_xref="InterPro:IPR019248" FT /db_xref="UniProtKB/TrEMBL:O30772" FT /protein_id="AAB71229.1" FT /translation="MSRKLSLLLVFLIFGSMLGANNIVKAEEPKPLNVIIVWHQHQPYY FT YDPVQGIYTRPWVRLHAANNYWKMAHYLTEFPDIHVTIDLSGSLIAQLADYMNGAKDIY FT QIISEKIANGEPLTYDEKWFMLQAPGGFFDHTIPWNGEPVTDENGNPIRDFWDRYTELK FT DKMLAAKQKYANLPLEEQKVAVTNEFTEQDYIDLAVLFNLAWIDYNYIISTPELKALYD FT KVDEGGYTREDLKTVLYHQMWLLNNTFKEHEKINLLLGNGNVEVTVVPYAHPIGPILND FT FGWSEDFDAHVKKAHELYKKYLGGGVATPRGGWAAESALNDKTLEILAENGWQWVMTDQ FT MVLERMGIPYSIENYYRPWVAEFNGKKIYLFPRNHDLSDRVGFRYSGMNQYEAVEDFIN FT ELLKIQKYNYDGSLVYVITLDGENPWEHYPYDGKLFLETLYKRLSELQEAGLIRTLTPT FT EYIQLYGDKANKLTPQMMERLDFTTEERVEALKVANSLGELYDLAGVTEEMQWPESSWI FT DGTLSTWIGEPQENYAWYWLYLARRTLMENKDKMDSASWEKAYEYLLRAEASDWFWWYG FT NDQDSGQDYSFDRYFKTYLYEIYKLAGVEPPSYLYGNYFPDGAPYTVRALEGLKEGDVK FT EYSSLSPVAEGVKVFFDSQGLHFIIKGRIDKFEISIYEKDKRIGNTFTLLQKKPDKIRY FT DVFPFVRDSVGLMITKHIVYKDGKAEIYNATDYEGYEKIGEAQVSVNGDEIEVIVPFEY FT LETPEDFYFAVSTVDELGMLEVITTPVNLKLPVQVKGVVLVDIADPEGDDHGPGTYTYP FT TDKVFVEGAFDLLRFRMLEQTDAYVMEFYFKELGGNPWKWA" XX SQ Sequence 3457 BP; 1086 A; 655 C; 826 G; 890 T; 0 other; atagggcttg gtgtaataaa gaacgttacc ctgaagtcag gggaagtcaa tgggttaaaa 60 atctcagaag gaaaattagc tggatttata agaataagag actctggaaa ggctgaactt 120 acagcgattg ggaagttcgt aaactcaaag ttcttccacc accttaggaa tagcttaatt 180 cttgcaacat taactgtgtt cctgacttta ctcttcgtag ttccggctgc atatgcattc 240 tcaaggctca agttctttgg aagagagcac atactatact tctacctgat gtttacccag 300 gtagcaggag gtttaggaat agcgggattg atagcccttt atggtatgct cgtaaagcta 360 ggcctctaca acaagcttcc agctttggcc ataatatacg ctgcaggtag cgttccattc 420 aacacttggc ttctcaaaag ttacatagac tctataagtc cagactttga cgaggccgca 480 cttgttgatg gagcaagcta tctccagata ataagatacg tattactccc aatggctctt 540 ccaggaattg ctacggtagc agttatgtca ttcataggag ggtggacaga attcatcctt 600 gcaagcctat tgttaaccga ggagaatcaa cctctctcag tgtggatata caccctaatg 660 ggtggaattg gaaggggaat tgactggaac tactttgcag ctgcagcgtt actatttgca 720 cttcccgtgt ttataatgtt catgcttgct caaaactaca ttagaagtgg cctaactgtc 780 ggtggattga aagaatgagg tggtggaaat gagtaggaag ctttctctcc tcttagtatt 840 tctaattttt ggaagcatgc tgggagcaaa caacattgta aaagctgagg agccaaagcc 900 attaaacgtc ataatagttt ggcaccaaca ccaaccctac tattatgatc ccgtgcaggg 960 catctatact aggccttggg ttaggctaca tgcggcaaat aactattgga agatggcaca 1020 ctacctaacg gaatttccag acattcacgt taccatagac ttatctgggt ctctgatagc 1080 tcagttagct gactacatga atggagcaaa ggacatctac cagataattt cggagaaaat 1140 cgccaatggt gagcctctaa cttatgatga gaaatggttt atgcttcagg ccccaggagg 1200 gttctttgat cacacaattc cttggaatgg agagcccgtt acagacgaga atggcaaccc 1260 aataagagac ttctgggata ggtatactga gcttaaagac aagatgttag ctgcaaagca 1320 aaagtatgcc aacctccctc tagaggagca aaaagttgca gttacaaacg aattcacgga 1380 gcaagattac attgaccttg cggttctctt caaccttgca tggatagatt acaattacat 1440 aatcagcact cctgaattaa aggccctcta cgacaaagtt gatgaaggag gatacacaag 1500 ggaagactta aagacagttc tctaccacca gatgtggttg ctaaacaata ctttcaagga 1560 gcatgagaaa ataaaccttc ttcttggaaa tggaaacgtt gaagttacag ttgttcctta 1620 cgctcaccca attggcccaa tacttaacga ctttggatgg agtgaagact ttgatgccca 1680 tgtaaagaag gctcacgagc tatacaagaa gtacctcgga gggggagtag caactcctag 1740 gggaggttgg gctgcagaat cggcacttaa tgacaagacg ttagaaattt tggctgagaa 1800 tggctggcaa tgggtaatga ctgatcaaat ggtgctggag agaatgggca ttccatatag 1860 cattgaaaac tattatagac cgtgggtagc ggagttcaat ggaaagaaga tatacttgtt 1920 cccaagaaat cacgatctaa gtgacagagt tggattcaga tacagcggaa tgaaccaata 1980 tgaggccgtt gaagacttca taaatgagct ccttaagatt caaaagtaca actatgatgg 2040 ttccttggtc tatgtaataa cattggatgg agagaatccc tgggagcact atccctatga 2100 tgggaaactc ttccttgaga cactgtacaa gaggctatca gagcttcaag aggctggttt 2160 aattagaact ctaactccca ccgagtacat tcagctctac ggagacaagg caaacaagct 2220 cactcctcag atgatggaga ggttggactt tactacagaa gagagagtag aagctcttaa 2280 agtggctaac tccctaggtg agctctatga tctcgcagga gttactgaag aaatgcagtg 2340 gccagagtca agttggattg atggaacgct ctcgacatgg ataggtgagc cacaagaaaa 2400 ctatgcatgg tattggctat atttagcgag aagaacctta atggagaaca aagacaaaat 2460 ggacagcgct tcctgggaaa aggcttatga gtacttactc agggctgagg caagtgactg 2520 gttctggtgg tatggaaatg atcaagatag tgggcaagat tactcctttg acagatactt 2580 caagacatat ctttacgaaa tctacaagtt agcaggagtt gagcctccaa gctatctcta 2640 tggaaactac ttcccagatg gtgctcctta cacagtaaga gccttagaag ggctaaaaga 2700 gggagatgtg aaagagtact caagcttatc tccagtggct gaaggggtaa aagtcttctt 2760 tgattcccag ggacttcact tcataataaa aggtagaatt gataagtttg aaataagcat 2820 ttatgagaag gacaagagga ttggcaacac ctttacactt ctacagaaga aacccgataa 2880 gattagatat gatgtgttcc catttgtaag ggatagcgtt gggttaatga taactaagca 2940 cattgtatac aaggatggaa aagctgaaat atacaacgcc actgactatg agggttatga 3000 gaagattgga gaggctcagg taagcgtaaa tggggatgaa attgaagtta ttgttccatt 3060 tgaataccta gagactccag aagacttcta cttcgcagtt tcaacagtag atgagctggg 3120 aatgttagag gtaataacta ctccagttaa cctcaaacta ccagtccaag taaaaggagt 3180 ggtgctggta gatatagcag atccagaggg agatgatcac gggccaggaa catacactta 3240 tccaacagac aaggtatttg tagagggggc ctttgacttg cttaggttca gaatgctaga 3300 acagaccgac gcatacgtca tggagtttta cttcaaggag cttggtggaa acccatggaa 3360 atgggcctaa tggattcagc ttgcaaatca ttgaagtgtt atcttgactt caaagaggga 3420 ggtaatactt cagcaatcaa gatgttccca gatggac 3457 // |
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