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EBI DbfetchID AF015445; SV 1; linear; genomic DNA; STD; PRO; 2580 BP. XX AC AF015445; XX DT 29-SEP-1997 (Rel. 52, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Bacillus sp. NG-27 alkaline thermostable endoxylanase gene, complete cds. XX KW . XX OS Bacillus sp. NG-27 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2580 RX DOI; 10.1128/AEM.66.6.2631-2635.2000 RX PUBMED; 10831448. RA Gupta N., Reddy V.S., Maiti S., Ghosh A.; RT "Cloning, expression, and sequence analysis of the gene encoding the RT alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic RT Bacillus sp. Strain NG-27"; RL Appl. Environ. Microbiol. 66(6):2631-2635(2000). XX RN [2] RP 1-2580 RA Ghosh A.; RT ; RL Submitted (22-JUL-1997) to the EMBL/GenBank/DDBJ databases. RL Molecular Biology, Institute of Microbial Technology, Sector 39-A, RL Chandigarh, U.T. 160 036, India XX FH Key Location/Qualifiers FH FT source 1..2580 FT /organism="Bacillus sp. NG-27" FT /strain="NG-27" FT /mol_type="genomic DNA" FT /clone="pGNG-17" FT /note="alkalophilic bacillus sp." FT /db_xref="taxon:65673" FT -35_signal 1099..1104 FT /note="part of promoter P2" FT -10_signal 1118..1123 FT /note="part of promoter P2" FT -35_signal 1161..1166 FT /note="part of promoter P1" FT -10_signal 1184..1189 FT /note="part of promoter P1" FT misc_feature 1189..1202 FT /note="catabolite repression site" FT RBS 1338..1341 FT sig_peptide 1351..1434 FT CDS 1351..2568 FT /codon_start=1 FT /transl_table=11 FT /product="alkaline thermostable endoxylanase" FT /EC_number="3.2.1.8" FT /db_xref="GOA:O30700" FT /db_xref="InterPro:IPR001000" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="PDB:2F8Q" FT /db_xref="PDB:2FGL" FT /db_xref="UniProtKB/TrEMBL:O30700" FT /protein_id="AAB70918.1" FT /translation="MLKTLRKPFIAGLALSLLLTGGASSVFAQGNGQAGPPKGGIFKEG FT EKGNGNVQPFAWQVASLADRYEESFDIGAAVEPHQLNGRQGKVLKHHYNSIVAENAMKP FT ISLQPEEGVFTWDGADAIVEFARKNNMNLRFHTLVWHNQVPDWFFLDEEGNPMVEETNE FT AKRQANKELLLERLETHIKTVVERYKDDVTAWDVVNEVVDDGTPNERGLRESVWYQITG FT DEYIRVAFETARKYAGEDAKLFINDYNTEVTPKRDHLYNLVQDLLADGVPIDGVGHQAH FT IQIDWPTIDEIRTSMEMFAGLGLDNQVTELDVSLYGWPPRPAFPTYDAIPQERFQAQAD FT RYNQLFELYEELDADLSSVTFWGIADNHTWLDDRAREYNDGVGKDAPFVFDPNYRVKPA FT FWRIID" FT misc_feature 2257..2290 FT /note="encodes active site; centered on glutamic acid which FT is involved in glycosidic bond cleavage by acting FT nucleophile" XX SQ Sequence 2580 BP; 853 A; 429 C; 577 G; 721 T; 0 other; aaccaaggag catatgtcat ttcagaagca acaggtgaaa tagaaggtat tttattggcg 60 tcaggttcag aagttcaatt agcggttgaa gcacagacgg cattagagaa agaaggaatt 120 catgtttctg ttgtaagtat gccgagctgg gagcgttttg aacaacaatc actagaatat 180 aagcaaagtg tgattcctcc acaagtcaaa gctcgtattg cagtagaaat gggatcgtct 240 cttggctggg agaaatacgt aggtgatcat ggggatatga ttacgattga tacgtttggt 300 gcatcagcgc ctgcaaacaa aatcttacac gagtacggct tcactgttga aaatgttgtg 360 tcgaaaatga aacaattgtt agggaagtaa gatgtttgaa tgacttaatc taacggacat 420 ctattccgct atattaataa aaagtttgat tttttaaatg ctaaaggaca tttgttccgt 480 tacattgaaa aataacatgg ttttctcgct ctttttactc aagtagcgga acgaatgtcc 540 gtaagaactg agaaaaggcg tctatttcgt gaaatagcgg aattgatgtc tgtaacgaag 600 gatcacatta acaccctctc tagaagaggg tgttttttta aaaaagataa gaaagcgtat 660 aaaatagttg acctgtggat aacattgcgc ttatatattc ttagaatgag ttaggggttt 720 gggaaactat gagacaagca taacccccga cccaagtgaa aaattcaaca cttgggtcgg 780 ggattatcgc attagtgatt ttgttgatta tcgtaattaa gcggccatga aagccgttat 840 ctgtgaacat ggctagcgtt ttggaaagtg agtggacaca ggagcagtta atcataatat 900 atcgctagga atagagttga ttttgcgtta ataagcgttc ctgtggccgc taaaaaacgg 960 aaacccgtat aatgttcaca aataacggct gctctgaccg caaaaatggt atgacacttt 1020 ctacacgttg tagccattag taatcatttc atagaacaat cctcccgctt tttttaaaac 1080 actttactag gactttagac aaatgactag gaaaaccttt tccagttgtt ggttctatat 1140 aattaatacc tagtattcat tttcagaaaa gtttgtttgt ttactagata aactaagtta 1200 aggttgttat aatgaaagcg aattcaaatc tagcttactt acatatgaaa gcctttacat 1260 caagaagcta gagcataacc cattggaaag gaggtttgtc agatattctc gtagttttac 1320 aaaaattcta ctaaaaaagg agattatcac atgctaaaaa cgttaagaaa acccttcatt 1380 gcaggactag ctttatcatt attactcact ggtggagcga gcagtgtatt tgctcaagga 1440 aacggtcaag ctggcccacc aaagggaggc atttttaaag aaggagaaaa aggaaatggc 1500 aatgtccaac cttttgcttg gcaagtggct tctcttgcag accgatatga agaatctttt 1560 gatattgggg cagcggttga gccgcaccaa ctgaatggaa gacaagggaa agtattaaag 1620 catcattaca atagtatcgt tgcagaaaat gcaatgaagc ccatttcgtt acaacctgaa 1680 gaaggtgtgt ttacttggga cggggcagat gcgattgttg agtttgctag aaaaaataat 1740 atgaacctac gtttccatac actcgtttgg cataaccaag tacctgattg gtttttctta 1800 gatgaagaag gcaatccaat ggtagaagaa acgaatgaag cgaaaagaca ggcgaataaa 1860 gaactattat tagaacgcct tgaaacacat attaaaacag tagtagaacg ctataaagac 1920 gatgtgactg cttgggatgt tgtcaatgaa gttgttgacg atggaacacc aaatgaaaga 1980 ggattgcgcg aatcagtttg gtaccaaatt acaggggatg aatacattcg agttgcgttt 2040 gaaacagccc gaaagtatgc aggtgaagac gcgaaattat ttattaacga ctacaatacg 2100 gaagtgacac cgaagcgtga ccacttatat aatctagttc aagacttact cgctgatgga 2160 gtaccaatcg atggagtcgg acaccaagca catattcaaa tcgattggcc aacaattgat 2220 gaaattcgaa cgtctatgga aatgtttgct ggcttaggac ttgataatca agtgacagag 2280 ttagatgtta gtttgtatgg atggccgcct cgtccagcat tcccaacata tgatgcaatt 2340 ccacaagaaa gatttcaagc tcaagccgac cgttataacc agttgtttga attatatgaa 2400 gagcttgatg cagatttaag cagtgtgaca ttctggggaa tcgcggataa ccatacatgg 2460 ttagatgacc gtgcaagaga atataatgat ggcgttggta aagatgcacc gtttgtattt 2520 gacccgaact atcgcgtcaa accagctttt tggagaatta tcgattaata gaagaagctt 2580 // |
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