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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AF014801; SV 1; linear; mRNA; STD; PLN; 1613 BP.
XX
AC   AF014801;
XX
DT   12-MAY-1998 (Rel. 55, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 2)
XX
DE   Eschscholzia californica (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1)
DE   mRNA, complete cds.
XX
KW   .
XX
OS   Eschscholzia californica (California poppy)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Ranunculales; Papaveraceae;
OC   Eschscholzioideae; Eschscholzia.
XX
RN   [1]
RP   1-1613
RX   DOI; 10.1046/j.1365-313X.1998.00085.x
RX   PUBMED; 9681018.
RA   Pauli H.H., Kutchan T.M.;
RT   "Molecular cloning and functional heterologous expression of two alleles
RT   encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl
RT   jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of
RT   benzylisoquinoline alkaloid biosynthesis";
RL   Plant J. 13(6):793-801(1998).
XX
RN   [2]
RP   1-1613
RA   Pauli H.H., Kutchan T.M.;
RT   ;
RL   Submitted (19-JUL-1997) to the EMBL/GenBank/DDBJ databases.
RL   Laboratorium fuer Molekulare Biologie, Universitaet Muenchen, Karlstrasse
RL   29, Muenchen 80333, Germany
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1613
FT                   /organism="Eschscholzia californica"
FT                   /mol_type="mRNA"
FT                   /note="methyl jasmonate-elicited cell suspension culture"
FT                   /db_xref="taxon:3467"
FT   CDS             37..1503
FT                   /codon_start=1
FT                   /gene="CYP80B1"
FT                   /product="(S)-N-methylcoclaurine 3'-hydroxylase"
FT                   /function="hydroxylase involved in the biosynthesis of
FT                   tetrahydrobenzylisoquinoline alkaloids in plants"
FT                   /note="cytochrome P-450-dependent monooxygenase; CYP80B1v2"
FT                   /db_xref="GOA:O64900"
FT                   /db_xref="HSSP:1DT6"
FT                   /db_xref="InterPro:IPR002401"
FT                   /db_xref="UniProtKB/Swiss-Prot:O64900"
FT                   /protein_id="AAC39453.1"
FT                   /translation="MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQL
FT                   GEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRV
FT                   KGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEE
FT                   VKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPM
FT                   LGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQ
FT                   INALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLP
FT                   YLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPL
FT                   TFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNG
FT                   MSVDHLIVEEKFGLTLQKEPPLFIVPKSRV"
XX
SQ   Sequence 1613 BP; 550 A; 269 C; 339 G; 454 T; 1 other;
     aagtccgagt gagaagcaga gttagagaaa aaaaaaatgg aggttgtcac agtagcactt        60
     attgcagtaa taatttcttc aatactctac ctcctctttg gtagtagtgg tcacaaaaat       120
     ctcccaccag gaccaaaacc atggccaata gtaggaaatc ttctccaact tggtgagaaa       180
     ccacacgctc aattcgccga actagctcaa acctatggtg acattttcac tcttaaaatg       240
     ggtactgaaa ctgtagttgt tgcatcaaca tcttcggcag cttccgaaat actaaaaacc       300
     catgatcgaa ttctatccgc tcgttacgtt tttcaaagtt ttcgagtaaa agggcatgta       360
     gaaaattcaa tagtttggtc agattgtact gaaacttgga agaatttaag aaaagtttgt       420
     aggacggaac ttttcacaca gaagatgata gaaagtcaag ctcatgttag agagaaaaaa       480
     tgtgaagaaa tggttgaata cttgatgaaa aaacaagggg aagaagtgaa aattgtggaa       540
     gtaatatttg gaacattagt gaatatattc ggaaatttga tattttcaca gaatatattt       600
     gaattgggtg anccaaatag tggaagttca gagttcaagg aatatctatg gaggatgttg       660
     gaattaggga attcaacaaa tccagctgat tattttccaa tgttgggtaa atttgatttg       720
     tttggacaga ggaaagaagt tgcagagtgt ttaaaaggga tttatgctat ttggggagct       780
     atgcttcaag aaaggaaatt agctaaaaaa gttgatggat accaaagcaa gaatgatttt       840
     gttgatgttt gtcttgattc tggacttaat gattatcaga tcaatgcctt gcttatggaa       900
     ttatttgggg caggcacaga aacaagcgca tcgacaattg agtgggccat gactgaacta       960
     acaaagaatc caaagataac agctaagctt agatcagaac ttcaaacagt ggtaggcgag      1020
     agatcggtaa aagaatccga cttccccaat cttccatacc ttgaagctac tgttaaagaa      1080
     accctaagac ttcacccacc aactccattg ctactcccac gtcgagcact tgaaacctgt      1140
     acaatcctca actacaccat cccaaaagat tgtcaaatta tggtcaacgc ttggggaatc      1200
     ggacgtgatc ccaagacttg gatcgatccg ttgactttct caccagagag attcttgaat      1260
     tctagtgttg actttagggg gaatgatttc agtttgatac catttggtgc aggaagaagg      1320
     atatgccccg gtctgccaat agcaaatcag tttattgcat tgctagtggc aacatttgtg      1380
     caaaatttgg attggtgtct accaaatggg atgagtgttg accatttgat agtggaggag      1440
     aagtttgggt tgactcttca aaaagaacca cctctattca ttgttcctaa atcaagggtt      1500
     tgatcttctc atcatggttc atgtgaaaac tagattatct attgtgaagg ctatgtatgt      1560
     tttctttcct tcctataaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa             1613
//
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