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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AF014800; SV 1; linear; mRNA; STD; PLN; 1901 BP.
XX
AC   AF014800;
XX
DT   12-MAY-1998 (Rel. 55, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 2)
XX
DE   Eschscholzia californica (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1)
DE   mRNA, partial cds.
XX
KW   .
XX
OS   Eschscholzia californica (California poppy)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Ranunculales; Papaveraceae;
OC   Eschscholzioideae; Eschscholzia.
XX
RN   [1]
RP   1-1901
RX   DOI; 10.1046/j.1365-313X.1998.00085.x
RX   PUBMED; 9681018.
RA   Pauli H.H., Kutchan T.M.;
RT   "Molecular cloning and functional heterologous expression of two alleles
RT   encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl
RT   jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of
RT   benzylisoquinoline alkaloid biosynthesis";
RL   Plant J. 13(6):793-801(1998).
XX
RN   [2]
RP   1-1901
RA   Pauli H.H., Kutchan T.M.;
RT   ;
RL   Submitted (19-JUL-1997) to the EMBL/GenBank/DDBJ databases.
RL   Laboratorium fuer Molekulare Biologie, Universitaet Muenchen, Karlstrasse
RL   29, Muenchen 80333, Germany
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1901
FT                   /organism="Eschscholzia californica"
FT                   /mol_type="mRNA"
FT                   /note="methyl jasmonate-elicited cell suspension culture"
FT                   /db_xref="taxon:3467"
FT   CDS             <1..1465
FT                   /codon_start=2
FT                   /gene="CYP80B1"
FT                   /product="(S)-N-methylcoclaurine 3'-hydroxylase"
FT                   /function="hydroxylase involved in the biosynthesis of
FT                   tetrahydrobenzylisoquinoline alkaloids in plants"
FT                   /note="cytochrome P-450-dependent monooxygenase; CYP80B1v1"
FT                   /db_xref="GOA:O64899"
FT                   /db_xref="HSSP:1DT6"
FT                   /db_xref="InterPro:IPR002401"
FT                   /db_xref="UniProtKB/Swiss-Prot:O64899"
FT                   /protein_id="AAC39452.1"
FT                   /translation="GTSTVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLG
FT                   EKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVK
FT                   GHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEV
FT                   KIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPML
FT                   GKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQI
FT                   NALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPY
FT                   LEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLT
FT                   FSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGM
FT                   SVDHLIVEEKFGLTLQKEPPLFIVPKSRV"
XX
SQ   Sequence 1901 BP; 625 A; 324 C; 390 G; 562 T; 0 other;
     cggcacgagc acagtagcac ttattgcagt aataatttct tcaatactat acctcctctt        60
     tggtggtagt ggtcacaaaa atctcccacc aggaccaaaa ccatggccaa tagttggaaa       120
     tctcctccaa cttggtgaga aaccacacgc tcaattcgcc gaactagctc aaacctatgg       180
     tgacattttc actcttaaaa tgggtactga aactgtagtt gttgcatcaa catcttcagc       240
     agcttccgaa atactaaaaa cccatgatcg aattctatcc gctcgttacg tttttcaaag       300
     ttttcgagta aaagggcatg tagaaaattc aatagtttgg tcagattgta ctgaaacttg       360
     gaagaattta agaaaagttt gtaggacaga acttttcaca cagaagatga tagaaagtca       420
     agctcatgtt agagagaaaa aatgtgaaga aatggttgaa tacttgatga aaaaacaagg       480
     ggaagaagtg aaaattgtgg aagtaatatt tggaacatta gtgaatatat ttggaaattt       540
     gatattttca cagaatatat ttgaattggg tgatccaaat agtggaagtt cagagttcaa       600
     ggaatatcta tggaggatgt tggaattggg gaattcaaca aatccagctg attattttcc       660
     aatgttaggt aaatttgatt tgtttggaca gaggaaagaa gttgcagagt gtttaaaagg       720
     gatttatgca atatggggag ctatgcttca agaaagaaaa ttagctaaaa aagttgatgg       780
     atataaaagc aagaatgatt ttgttgatgt ttgtcttgat tctggactta atgattatca       840
     aatcaatgcc ttgcttatgg aattatttgg ggcaggcaca gaaacgagcg catcgacaat       900
     tgagtgggcc atgactgaac taacaaagaa tccaaagata acagctaaga ttagatcaga       960
     aattcaaaca gtggtaggcg agagatcggt aaaagaatcc gacttcccca atcttccata      1020
     ccttgaagct actgttaaag aaaccctaag acttcaccca ccaactccat tgctactccc      1080
     acgccgagca cttgaaacct gtacaatcct caactatacc atcccaaaag attgtcaaat      1140
     tatggtcaac gcttggggaa tcggtcgtga tcccaagact tggaccgatc cgttgacttt      1200
     ctcaccagag agattcttga attctagtgt tgactttagg gggaatgatt tcagtttgat      1260
     accatttggt gcaggaagaa ggatatgccc cggtctgcca atagcaaatc agtttattgc      1320
     attgctagtg gcaacatttg tgcaaaattt ggattggtgt ctaccaaatg ggatgagtgt      1380
     tgaccatttg atagtggagg agaagtttgg gttgactctt caaaaagaac cacctctatt      1440
     cattgttcct aaatcaaggg tttgatcttt ctcatcatgg ttcatgtgaa aactagatta      1500
     tcttttgttt tggctatgca tctgttttct ttccttccta taagcttttg ttcaccaagt      1560
     aagaaaatgt ccacacgatt tttgttcggc cgaggtccga gggtatcaag actatggaca      1620
     acaatcctct gagacaacta aggggtcact ggatcaaaat tgtcgatatg tacattttct      1680
     tctgaacaag gttacacatt ctagctatta ggtgtcgcgc taataaaata taataatcac      1740
     ttttgatagt tttgtagaga catgttatgc aatacatatc tattgcaata cgcatgctca      1800
     taatgcatat acatatttga aaagttgtgg atgttgttcc atgttcaacc ataaagcgag      1860
     tgttttacta tgttaaagtg aaaaaaaaaa aaaaaaaaaa a                          1901
//
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