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EBI DbfetchID AF013169; SV 2; linear; genomic DNA; STD; PRO; 5720 BP. XX AC AF013169; U37557; XX DT 07-JUL-1998 (Rel. 56, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Pyrococcus furiosus beta-glucosidase (celB) gene, complete cds; adh-lam DE operon, complete sequence; biotin ligase BirA homolog (birA) gene, complete DE cds; and 2-phosphoglycerate kinase (pgk) gene, partial cds. XX KW . XX OS Pyrococcus furiosus OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-1732 RX PUBMED; 8522516. RA Voorhorst W.G., Eggen R.I., Luesink E.J., de Vos W.M.; RT "Characterization of the celB gene coding for beta-glucosidase from the RT hyperthermophilic archaeon Pyrococcus furiosus and its expression and RT site-directed mutation in Escherichia coli"; RL J. Bacteriol. 177(24):7105-7111(1995). XX RN [2] RP 3676-4615 RX DOI; 10.1074/jbc.272.50.31258 RX PUBMED; 9395451. RA Gueguen Y., Voorhorst W.G., van der Oost J., de Vos W.M.; RT "Molecular and biochemical characterization of an endo-beta-1,3- glucanase RT of the hyperthermophilic archaeon Pyrococcus furiosus"; RL J. Biol. Chem. 272(50):31258-31264(1997). XX RN [3] RP 1-5720 RX PUBMED; 10368153. RA Voorhorst W.G., Gueguen Y., Geerling A.C., Schut G., Dahlke I., Thomm M., RA van der Oost J., de Vos W.M.; RT "Transcriptional regulation in the hyperthermophilic archaeon Pyrococcus RT furiosus: coordinated expression of divergently oriented genes in response RT to beta-linked glucose polymers"; RL J. Bacteriol. 181(12):3777-3783(1999). XX RN [4] RP 1743-3675 RA Voorhorst W.G.B., Wittenhorst V., Gueguen Y., van der Oost J., Vos W.M.; RT "Genetic and biochemical characterization of a short-chain and an RT iron-containing alcohol dehydrogenase from the hyperthermophilic archaeon RT Pyrococcus furiosus"; RL Unpublished. XX RN [5] RC Nucleotide sequence updated by submitter RP 1-1732 RA Voorhorst W.G.B.; RT ; RL Submitted (02-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL Microbiology, Wageningen Agricultural University, Hesselink van RL Suchtelenweg 4, Wageningen NL-6703CT, The Netherlands XX RN [6] RP 1-5720 RA Gueguen Y., Voorhorst W.G.B., van der Oost J., Geerling A.C.M., RA de Vos W.M.; RT ; RL Submitted (09-JUL-1997) to the EMBL/GenBank/DDBJ databases. RL Laboratory of Microbiology, Wageningen Agricultural University, Hesselink RL van Suchtelenweg 4, Wageningen NL-6703CT, The Netherlands XX RN [7] RC Sequence update by submitter RP 1-5720 RA van der Oost J.; RT ; RL Submitted (20-JUL-2004) to the EMBL/GenBank/DDBJ databases. RL Laboratory of Microbiology, Wageningen Agricultural University, Hesselink RL van Suchtelenweg 4, Wageningen NL-6703CT, The Netherlands XX CC On Jul 20, 2004 this sequence version replaced gi:3288807. XX FH Key Location/Qualifiers FH FT source 1..5720 FT /organism="Pyrococcus furiosus" FT /strain="DSM 3638" FT /mol_type="genomic DNA" FT /db_xref="taxon:2261" FT gene complement(194..1612) FT /gene="celB" FT CDS complement(194..1612) FT /codon_start=1 FT /transl_table=11 FT /gene="celB" FT /product="beta-glucosidase" FT /function="hydrolysis of beta-1, 4 linked FT di-/oligo-saccharides of glucose, galactose, and fucose" FT /note="family 1 glycosyl hydrolase" FT /db_xref="GOA:Q51723" FT /db_xref="HSSP:1QVB" FT /db_xref="InterPro:IPR018120" FT /db_xref="UniProtKB/TrEMBL:Q51723" FT /protein_id="AAC25555.1" FT /translation="MKFPKNFMFGYSWSGFQFEMGLPGSEVESDWWVWVHDKENIASGL FT VSGDLPENGPAYWHLYKQDHDIAEKLGMDCIRGGIEWARIFPKPTFDVKVDVEKDEEGN FT IISVDVPESTIKELEKIANMEALEHYRKIYSDWKERGKTFILNLYHWPLPLWIHDPIAV FT RKLGPDRAPAGWLDEKTVVEFVKFAAFVAYHLDDLVDMWSTMNEPNVVYNQGYINLRSG FT FPPGYLSFEAAEKAKFNLIQAHIGAYDAIKEYSEKSVGVIYAFAWHDPLAEEYKDEVEE FT IRKKDYEFVTILHSKGKLDWIGVNYYSRLVYGAKDGHLVPLPGYGFMSERGGFAKSGRP FT ASDFGWEMYPEGLENLLKYLNNAYELPMIITENGMADAADRYRPHYLVSHLKAVYNAMK FT EGADVRGYLHWSLTDNYEWAQGFRMRFGLVYVDFETKKRYLRPSALVFREIATQKEIPE FT ELAHLADLKFVTRK" FT gene 1788..4581 FT /gene="adh-lam operon" FT gene 1788..2492 FT /gene="adhA" FT CDS 1788..2492 FT /codon_start=1 FT /transl_table=11 FT /gene="adhA" FT /product="short chain alcohol dehydrogenase" FT /function="NADP(H)-dependent alcohol dehydrogenase activity FT with primary and secondary alcohols as substrates, highest FT activity with 2-pentanol" FT /db_xref="GOA:O73949" FT /db_xref="HSSP:1IOL" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/TrEMBL:O73949" FT /protein_id="AAC25556.1" FT /translation="MKVAVITGASRGIGEAIAKALAEDGYSLALGARSVDRLEKIAKEL FT SEKHGVEVFYDYLDVSKPESVEEFARKTLAHFGDVDVVVANAGLGYFGRLEELTEEQFH FT EMIEVNLLGVWRTIKAFLNSLKRTGGVAIVVTSDVSARLLPYGGGYVATKWAARALVRT FT FQIENPDVRFFELRPGAVDTYFGGSKAGKPKEQGYLKPEEVAEAVKYLLRLPKDVRVEE FT LMLRSIYQKPEY" FT gene 2503..3675 FT /gene="adhB" FT CDS 2503..3675 FT /codon_start=1 FT /transl_table=11 FT /gene="adhB" FT /product="iron containing alcohol dehydrogenase" FT /function="NADP(H)-dependent alcohol dehydrogenase activity FT with methanol as substrate" FT /db_xref="GOA:O73950" FT /db_xref="HSSP:1O2D" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/TrEMBL:O73950" FT /protein_id="AAC25557.1" FT /translation="MFEISIYLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTK FT ETGVLYRVQELLKQAGVESIVFDKIIPNPISTHVDEGAEIARKENVSFVVGLGGGSAID FT SAKAIAMTAASGGKYWDYVPAVGGGKKPTGALPIVAIPTTHGTGTEADPYAVITNPETK FT EKQGIGYDVLFPKFSIVDPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLAL FT DSMRRIVTYLPLAYENLRNLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEV FT PHGLGLCILGPYLFEYILDYAYEKLAIVGREVFGVYEPNDRKAAELAIKKLRDFQSLFG FT VNKKLRELGVKEEDIPEMARTAYRMMKPVIEATPGDLKVEDLEEIYRRAY" FT gene 3688..4581 FT /gene="lamA" FT CDS 3688..4581 FT /codon_start=1 FT /transl_table=11 FT /gene="lamA" FT /product="endo-beta-1,3-glucanase precursor" FT /function="hydrolysis of beta-1,3 linked glucose polymers FT (laminarin); some hydrolysis of beta-1,3-1,4 linked glucose FT polymers (lichenan, barley glucan)" FT /note="laminase; family 16 glycosyl hydrolase" FT /db_xref="GOA:O73951" FT /db_xref="InterPro:IPR013320" FT /db_xref="PDB:2VY0" FT /db_xref="UniProtKB/TrEMBL:O73951" FT /protein_id="AAC25554.2" FT /translation="MKKEALLFLSLIFLVFVSGCIHHSTNQQLSSKQQVPEVIEIDGKQ FT WRLIWHDEFEGSEVNKEYWTFEKGNGIAYGIPGWGNGELEYYTENNTYIVNGTLVIEAR FT KEIITDPNEGTFLYTSSRLKTEGKVEFSPPVVVEARIKLPKGKGLWPAFWMLGSNIREV FT GWPNCGEIDIMEFLGHEPRTIHGTVHGPGYSGSKGITRAYTLPEGVPDFTEDFHVFGIV FT WYPDKIKWYVDGTFYHEVTKEQVEAMGYEWVFDKPFYIILNLAVGGYWPGNPDATTPFP FT AKMVVDYVRVYSFVSG" FT sig_peptide 3688..3747 FT /gene="lamA" FT /note="putative" FT mat_peptide 3748..4578 FT /gene="lamA" FT /product="endo-beta-1,3-glucanase" FT gene 4623..5330 FT /gene="birA" FT CDS 4623..5330 FT /codon_start=1 FT /transl_table=11 FT /gene="birA" FT /product="biotin ligase BirA homolog" FT /function="probable function in biotin incorporation" FT /note="contains biotin ligase domain with no DNA binding FT domain" FT /db_xref="GOA:O73952" FT /db_xref="HSSP:1BIA" FT /db_xref="InterPro:IPR004408" FT /db_xref="UniProtKB/TrEMBL:O73952" FT /protein_id="AAC25558.1" FT /translation="MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMG FT HGRIGRRWESPPGGLWMSVVLHPRISTEDLPKLSFLGALAVVRTLSEFSIQGKIKWPND FT VLVDYKKIAGILVEKRGDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRALVTN FT LDELYKMFLSSPASLLEMAKKEMILGVPVRIIGDGEIEGIAEDIDELGRLILRLSDGSL FT KKIVYGDVSLRFL" FT gene complement(5313..>5720) FT /gene="pgk" FT CDS complement(5313..>5720) FT /codon_start=1 FT /transl_table=11 FT /gene="pgk" FT /product="2-phosphoglycerate kinase" FT /db_xref="GOA:Q8U4K7" FT /db_xref="InterPro:IPR005144" FT /db_xref="UniProtKB/Swiss-Prot:Q8U4K7" FT /protein_id="AAC25559.1" FT /translation="QVNAVAVGVNAVIQRAIKEGLNAIIEGIHLVPGFIKIDYEMAFMY FT MIVARSREELEARFYERTRYSKRSAQYYISHLDEIMEIQEYLIKKAREYRVPIIENVEL FT EKTISTIMEDIMEKTVEIMKKKGLDMLEEPK" XX SQ Sequence 5720 BP; 1636 A; 1096 C; 1289 G; 1699 T; 0 other; gggatactac caataaaatc accttgctat ggatttataa actttatgac tgatcatcca 60 tatgtatgta gaatccatgg aaattgaggg ctatcaatct ttgctatttt tatggcatgg 120 tttctcttgt taagctttta tgatcttatt ttcccaaaaa tatgagttaa aaaacaaaga 180 aaaaatgaaa tggctacttt cttgtaacaa atttgaggtc tgcgaggtga gctaattctt 240 ctggaatttc tttttgagtg gctatttctc tgaataccag ggcgcttggc cttaaatatc 300 tcttctttgt ctcgaaatcc acgtaaacca atccaaatct catcctgaac ccttgggccc 360 attcgtagtt gtctgttaga gaccagtgga gataccctct aacatcagca ccttctttca 420 tagcattgta aactgccttt agatggctta cgagatagtg tggcctgtat ctatctgctg 480 catcggccat accgttctct gtaattatca ttggtagctc gtaggcattg tttaaatact 540 taagaaggtt ctcaaggccc tctgggtaca tttcccatcc aaagtcacta gcaggtcttc 600 ctgactttgc aaatcctcct ctctcactca taaatccata tccaggtaaa ggaactaggt 660 gtccatcttt ggctccatat accagcctgg agtagtagtt tacgccgatc cagtctagct 720 ttccttttga gtgtagaatt gttacaaact catagtcttt ctttctgatt tcctctactt 780 catccttata ctcctccgct agaggatcgt gccaagcaaa ggcgtatatc actcccacgg 840 atttttctga atactctttt atggcatcat atgctccgat gtgagcctga attaagttga 900 attttgcctt ttctgctgct tcaaagctta gatatcctgg tggaaatcct gaacgtagat 960 taatgtaacc ttgattgtag actacgtttg gttcgttcat tgtgctccac atgtcaacga 1020 ggtcatcaag gtgataagca acgaaggcgg caaacttcac aaactctacc actgtcttct 1080 catctaacca tcctgcagga gccctatccg ggccaagttt ccttactgca attgggtcat 1140 gaatccataa tggaagaggc cagtggtaga ggtttaatat gaaggttttg cccctctcct 1200 tccagtctga gtaaatcttg cgataatgtt caagggcctc catgttggca attttctcta 1260 gctcttttat tgtactctct ggaacgtcta cggaaattat gttgccttct tcatcctttt 1320 ccacatcaac tttaacgtca aatgttggct ttggaaaaat tcttgcccac tcaatgccac 1380 ctctaataca atccattcct agcttttctg caatgtcatg atcttgctta tagaggtgcc 1440 aatatgctgg gccgttctct ggtagatctc cacttactag acctgatgct atgttctcct 1500 tgtcgtgaac ccacacccac cagtcgcttt ccacttcact tcctggcagt cccatctcaa 1560 actggaaacc agaccaagaa tatccaaaca tgaagttttt tgggaacttc atactttcca 1620 cctctagctt tatattttga tattgtgatt tataatattt cccatgcaat tataaatgtg 1680 ggaagataga tatatgttta aacttgcttt ttgaggaggc tcaattctct tcataaatgt 1740 ccaaaaatta taaaaaacat caagcttata ttgctgggag ggataaaatg aaggttgccg 1800 taattactgg ggcatcccgt ggaatcgggg aagctatagc aaaggccctt gctgaagatg 1860 gatattccct tgccttaggg gctagaagtg ttgataggtt agagaagatt gccaaggaac 1920 tcagcgaaaa acatggggtg gaggtatttt acgactacct cgatgtatca aaaccagaaa 1980 gcgttgaaga gtttgcaagg aaaacgctag ctcactttgg agatgtggac gttgttgtgg 2040 ccaatgcggg gcttggttac tttggtaggc ttgaagagct tacagaagag cagttccacg 2100 aaatgattga agtaaacctt ttgggagttt ggagaacaat aaaagctttc ttaaactcct 2160 taaagcggac tggaggagtg gctattgttg ttacttcaga tgtttctgca aggctacttc 2220 catacggtgg aggttatgtg gcaactaaat gggctgcaag agcattggta aggaccttcc 2280 agattgagaa tccagatgtg aggttcttcg agctaagacc tggagcagta gatacatatt 2340 ttggagggag caaagctggg aagccaaagg agcaagggta tttaaaacct gaggaagttg 2400 ctgaggcagt aaaatacctc ctaagacttc caaaggatgt tagggttgag gaattaatgt 2460 tgcgctcaat ttatcaaaaa cctgagtatt gaggtggtgt gcatgtttga gatatcaatt 2520 tatcttccca cagaaatagt ttttggtcct gggaagcttg aaatgcttcc taaactagtg 2580 aagaagcatg ggctttctgg gaaggcccta atagtaactg gaaggagaag cacaaaggaa 2640 actggagttc tttatagagt tcaagaacta cttaagcaag ctggggtaga gagcatagtt 2700 tttgacaaaa ttattccaaa tccaatatct actcatgtgg atgaaggggc agagatagcg 2760 agaaaagaaa atgttagctt tgttgttggc ttgggtggtg gaagtgcgat agatagtgca 2820 aaagctatag caatgactgc cgccagtgga ggtaaatatt gggactatgt tccagctgtg 2880 ggaggaggaa agaagcctac tggagcgctt ccaatagttg caattccaac aacccacggg 2940 actggaacgg aggctgatcc ttatgctgtt ataactaatc ctgaaacaaa ggagaagcag 3000 ggaattggat atgatgttct cttccccaaa ttctctatag ttgatccaga acttatgctt 3060 actcttccaa aagatcaaac agtgtacact tcaatggatg ctttctacca ctccattgag 3120 gcctttctta atgttagagc aaatccatat tcggatgttc tggctctcga ctcaatgagg 3180 cgcattgtta cataccttcc attggcctac gaaaacttga gaaatcttga agcaagaacg 3240 caacttgcct gggcaagtac tgaggctgga ataacggaaa cggtaacggg agttgtggca 3300 aatcatgcac ttgagcatgg tctaagtgga ttctatcctg aagtgcctca tggtctgggc 3360 ctctgcattc taggacccta cctctttgaa tacattctcg actatgccta tgaaaagttg 3420 gcgatagtcg gaagagaggt atttggagtt tacgagccaa atgacagaaa ggcagcagag 3480 ctagctatta agaagctacg tgacttccag agcctctttg gagtaaacaa gaagctcaga 3540 gaattagggg ttaaagagga agacattcca gagatggcta ggactgctta tagaatgatg 3600 aaacctgtta tagaggcaac accgggagat ttgaaagttg aagacttgga agagatctat 3660 agaagagcat actaacgggc tgacacaatg aaaaaagaag ccttgttgtt tctttcctta 3720 atttttctcg tgtttgtaag tggatgtatt catcattcaa ctaatcaaca actttcttct 3780 aaacaacaag tccctgaagt gatagaaata gatggaaaac agtggagact tatttggcac 3840 gatgagtttg aaggttccga agtaaataaa gaatactgga catttgagaa gggaaatgga 3900 atagcttatg gaatcccggg atgggggaat ggagagcttg aatactacac ggaaaacaac 3960 acctatattg taaatggcac ccttgtcatt gaggccagaa aagaaataat tactgatcct 4020 aacgaaggaa cgtttctcta cacttcatca agacttaaga ctgaaggtaa ggtagaattt 4080 agccctccag tagttgttga ggctagaata aagcttccaa aaggtaaagg tttatggcct 4140 gcattctgga tgttggggag caacataagg gaagtaggct ggccaaattg tggagaaata 4200 gacataatgg agttccttgg ccatgagcca cggacaattc acggaactgt tcatggccca 4260 ggttactcgg gaagtaaagg aattactagg gcctatacac tccctgaagg tgttccagac 4320 tttacagaag acttccatgt atttggaata gtttggtatc cggataaaat aaagtggtac 4380 gttgatggaa ctttttatca tgaggttaca aaagaacaag tggaggctat gggctatgag 4440 tgggtcttcg ataagccctt ctatataatc cttaatcttg cagtgggtgg ttattggcca 4500 ggaaaccccg atgctacaac tccatttcca gcaaagatgg tggtggatta tgtaagggtt 4560 tactcattcg ttagtggttg aggttgggaa agattttatt gctttgttga gcataaaata 4620 atatgctggg cttgaacact tcaattattg ggaaggtggt tatatactat agagaagtca 4680 catccacaaa tgaagtagca aagctcggca attttgaaga aggaactgta atagttgctg 4740 acactcaaac tatgggacat ggaagaattg gtaggaggtg ggaatctcca cctggcgggc 4800 tgtggatgtc cgtggttttg catcctagaa tttctaccga ggatctcccg aaattgagct 4860 ttctaggggc attagcagtt gttaggactc tctctgaatt ttccattcag gggaagataa 4920 agtggccaaa cgatgttctt gttgattaca agaaaatagc tggaatactc gttgagaaaa 4980 gaggggataa cgttgtgctt ggagtagggc taaacgtaaa caatgaagcc ccagagaatg 5040 ccacgtctat ggccgagata ttgggaactt ccgtaccttt aattgaggtt ttccgagcat 5100 tggtgactaa cttagatgaa ttgtacaaga tgtttttgtc ttccccagca agccttcttg 5160 agatggcaaa aaaggaaatg attcttggag ttcccgttag aattattggg gatggagaaa 5220 ttgaaggcat tgctgaagac atcgacgagc ttggaagact aattctaagg ctctcagatg 5280 gaagcctaaa gaaaattgtt tatggagatg tgtcacttag gttcctctag catgtctagt 5340 cctttcttct tcattatttc tacagttttt tccataatgt cctccatgat tgtggagatt 5400 gttttttcaa gctctacatt ctcaattatg ggaactctat attcccttgc tttctttatc 5460 aagtattcct ggatttccat tatctcatct aagtgggaga tatagtattg ggcagatctt 5520 ttgctgtatc tagttctttc ataaaatctg gcttccaact cctccctact tctagccact 5580 atcatgtaca taaaggccat ctcataatca attttaatga accctgggac taaatgtatt 5640 ccctctatta tagcgtttag gccttctttt atggccctct gaattactgc attaactccc 5700 acggcaacag catttacctg 5720 // |
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