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EBI DbfetchID AF007559; SV 1; linear; genomic DNA; STD; PRO; 4425 BP. XX AC AF007559; XX DT 04-AUG-1998 (Rel. 56, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Cytophaga drobachiensis kappa-carrageenase precursor (cgkA) gene, complete DE cds. XX KW . XX OS Zobellia galactanivorans OC Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales; OC Flavobacteriaceae; Zobellia. XX RN [1] RP 1-4425 RX PUBMED; 9580981. RA Barbeyron T., Gerard A., Potin P., Henrissat B., Kloareg B.; RT "The kappa-carrageenase of the marine bacterium Cytophaga drobachiensis. RT Structural and phylogenetic relationships within family-16 glycoside RT hydrolases"; RL Mol. Biol. Evol. 15(5):528-537(1998). XX RN [2] RP 1-4425 RA Barbeyron T.; RT ; RL Submitted (10-JUN-1997) to the EMBL/GenBank/DDBJ databases. RL CNRS, Place Georges Teissier, Roscoff, Bretagne/Finistere 29680, France XX DR StrainInfo; 163368; Dsij. XX FH Key Location/Qualifiers FH FT source 1..4425 FT /organism="Zobellia galactanivorans" FT /strain="Dsij" FT /mol_type="genomic DNA" FT /note="the taxonomic position of 'Cytophaga' drobachiensis FT is unclear, but it appears to belong in the FT Flavobacteriaceae" FT /db_xref="taxon:63186" FT -35_signal 591..596 FT /gene="cgkA" FT -10_signal 612..617 FT /gene="cgkA" FT RBS 856..869 FT /gene="cgkA" FT /note="Shine-Dalgarno sequence is replaced by an omega-like FT sequence" FT sig_peptide 875..979 FT /gene="cgkA" FT CDS 875..2512 FT /codon_start=1 FT /transl_table=11 FT /gene="cgkA" FT /product="kappa-carrageenase precursor" FT /function="hydrolyses the beta-(1,4) linkage in the FT poly(-4-O-alpha-3,6-anhydro-D-galactopyranosyl-(1, FT 3)-4-O-sulfate-beta-D-galactopyranose)" FT /EC_number="3.2.1.83" FT /note="belongs to the family 16 of glycoside hydrolases" FT /db_xref="GOA:O84907" FT /db_xref="HSSP:1DYP" FT /db_xref="InterPro:IPR000757" FT /db_xref="InterPro:IPR008985" FT /db_xref="InterPro:IPR013320" FT /db_xref="UniProtKB/TrEMBL:O84907" FT /protein_id="AAC27890.1" FT /translation="MKKPNFYGKMGRTALSSLFYLFFLGLVYGQQPTKTSNPNDQWTIK FT WSASDEFNKNDPDWAKWIKTGNLPNTSAWKWNNQKNVKISNGIAELTMRHNANNTPPDG FT GTYFTSGIFKSYQKFTYGYFEAKIQGADIGEGVCPSFWLYSDFDYSVANGETVYSEIDV FT VELQQFDWYEGHQDDIYDMDLNLHAVVKENGQGVWKRPKMYPQEQLNKWRAMDPSKDFH FT IYGCEVNQNEIIWYVDGVEVARKPNKYWHRPMNVTLSLGLRKPFVKFFDNKNNAINPET FT DAKAREKLSDIPTSMYVDYVRVWEKSAGNTTNPPTSEVGTLKTKGSKLVIDHWDASTGT FT ISAVSNNTKTGQYAGSVNNASIAQIVTLKANTSYKVSAFGKASSPGTSAYLGISKASNN FT ELISNFEFKTTSYSKGEIEIRTGNVQESYRIWYWSSGQAYCDDFNLVEINSGASQLNEN FT ETETALEKGIHIYPNPYKNGPLTIDFGKPFSGEVQITGLNGRTFLRRNVVDQTSVQLLE FT SKSKFKSGLYIVKISGPDGEVSKKILVE" FT mat_peptide 980..2509 FT /gene="cgkA" FT /product="kappa-carrageenase" FT terminator 2542..2567 XX SQ Sequence 4425 BP; 1333 A; 991 C; 863 G; 1238 T; 0 other; gcctccgtat tcgacaatgt tgtacgatgc ttggcgattc ggactctgtt taagcactcg 60 atttcgtaaa ggcactatcc actcattcat tccgactcaa tattcttttc gacaaatgca 120 accggttcca ttgaaaaggc cctaaaaata cagctttccc gccccccatc gtagaaggtt 180 ccaatatgct tcaacccctt tttcagcctt acttcagggg tattactttc atgcctaggg 240 ccgcaaatac attcgcttgg acccagtcac ctatataatt gaatacggaa ctacccatgg 300 cttccttccc tttgggaacc tatggtacag acttgccttt tttaaaccgg ttacttcagc 360 taattcgcca agctggttcc ttcataacct ttggcccgaa acaccttgca agcacataaa 420 tcttatccaa tattttgcgg tctcatggga caaatctata acaaacattc aattttacca 480 aacgttcggt aataaatcta gtcaaaaacg gggtccgatt cattttagaa gaaaggtaaa 540 gcccccaaaa gagcggttta cttgaagata tgatttataa aacacaataa gtgacaaagg 600 aagatcatgg ctataattag ttgaaaaaac agggcttacc atgacatgga gctttattga 660 aaacagatgt ccaacaagaa taaaggaggg ccgttcgacc gcgacgttta aataaaaaca 720 tattccatat caaaatttaa ttaaggttct ttcctacagt atttataaga aattactaaa 780 attagttagg ataatactac aaaatggtaa aattggatta ctcagattga accatagcct 840 ctactttagt cggctaacaa aaacaattat agtaatgaaa aaaccaaatt tttatggcaa 900 gatgggtaga actgcacttt caagtctttt ctacctcttt ttcctaggcc ttgtgtatgg 960 gcaacaacct acgaagactt caaatccgaa cgatcagtgg accatcaaat ggagtgcttc 1020 ggacgaattc aacaaaaatg accccgactg ggcaaaatgg atcaagacag gaaaccttcc 1080 gaatacatcg gcatggaaat ggaacaatca aaaaaacgta aagatttcca acggaattgc 1140 ggaactaacg atgaggcata acgccaataa taccccacct gacggaggaa cctatttcac 1200 ctctgggata tttaagtcgt accaaaaatt tacgtatgga tactttgagg ccaaaatcca 1260 aggagcggat ataggtgaag gcgtatgccc atcgttttgg ctttatagtg atttcgacta 1320 ttccgtagcc aatggggaaa cggtatacag tgaaatagat gtagttgaac tacaacaatt 1380 cgattggtat gaaggccatc aggacgacat ttacgacatg gacttaaatc tacacgccgt 1440 tgtcaaagaa aacggacagg gggtttggaa aaggccaaaa atgtaccctc aagaacagtt 1500 gaacaaatgg agagccatgg acccgagtaa agactttcat atctatggtt gtgaagtgaa 1560 ccagaacgaa atcatatggt atgttgacgg tgtcgaggtt gcccgaaaac caaataaata 1620 ttggcatcgc cccatgaacg ttaccctttc attgggactc agaaaaccat ttgtgaaatt 1680 tttcgacaat aagaacaatg ccataaatcc agaaaccgat gccaaggcaa gggaaaaatt 1740 atcggatata cctacatcga tgtatgtgga ttacgttcgg gtctgggaaa aatcagcagg 1800 taacactacc aatcccccaa ccagcgaggt cggcacacta aaaacaaagg gttcgaaact 1860 ggtgattgac cattgggatg caagtacagg gactatttcg gctgtcagta acaatacaaa 1920 gacaggtcaa tatgccggtt cagtgaacaa cgcgagcatc gcccagatag taacattaaa 1980 agcgaatact tcatataagg tatcggcttt cggaaaggcc agctcacccg gaacatcggc 2040 ttatctaggc attagtaaag catccaacaa cgaactcata agcaattttg aattcaaaac 2100 aacctcatac tccaaaggcg agattgagat aagaactgga aatgttcagg aatcatatcg 2160 catatggtat tggtcttccg ggcaagccta ttgcgatgat tttaaccttg ttgaaataaa 2220 cagcggggct tcacaactca atgaaaatga gactgaaaca gcactggaaa aaggtataca 2280 catttatccg aatccctata aaaacggtcc attgacaatc gattttggca aacccttcag 2340 cggcgaggtc caaatcaccg gtttaaacgg tagaacattc ttaagaagaa atgttgtcga 2400 tcaaacttcg gttcagctcc tagaatccaa atctaaattc aagagcggtc tatatatcgt 2460 taaaattagt ggcccggatg gagaggtttc aaaaaagata ctcgtggagt aactaaaaat 2520 caatttttac aggattacag acgggcaaag ggattttcct ttgcccgttt ttaaaattat 2580 gggcggaaac gattgttgcg acttttaccc ttaacacaga cctgctttcc ttttatacgc 2640 aacattagtt atgtagaggt tcgatgcgtt cttcgatatc cgcgacgaat ttaggatggc 2700 atcactagag atcaaatagt tggtcgttcc atatttttta tccatttgcg ccgctatgcc 2760 ctctaacttt cgaagacttt gggacaatgc actacggttt ttccccgttg gcggtctcta 2820 ttgatgatgt atctcagata ggcttctata tggggcatac taagtttcac aaagtcttcc 2880 ataagcaaaa attgcccctt taccttttga tactcttttt ttgagaggaa ttttttcacc 2940 gattctatat ctgaaatcga ttcatggcat aacaccaagg cctcttcacg ttcggccatt 3000 gcatttttat aatctcttcg gttgggtgat agacgaatgc ctcgatcaaa gcattgttct 3060 ttatatcatc aggattccat ttgaccattg attttccggt atgaagcact tgggccgtca 3120 tataatcaag cgaggccatc ttactgtgtt cctgcaccca aactccctta aagtaataca 3180 cggagttagc gatatcgttg gtccgcttta gagccttgac catagctggt gccgcctcct 3240 ttccgaattt atccatcgcc caatcgtccc acactttccc tacatctaca taagggtctt 3300 tcgcaaagcg tgacagggca tacagattta cttcattgta attttcgaac agggtctggt 3360 ccttttccca ataggtacgc atggccaaag tacgtatggc cttgttgtgg cttaaggcat 3420 gctgaaccct cacctgtatc tcttccggaa aacaataagg aataaaagac ctcccccaat 3480 attcccctac accactaaac catagggagg ccctggtatc cgtttccatc attatacgat 3540 tcggcccttt atacgggtcc caatcacccg actgggactt atgcaccatc aaaaaggact 3600 catttctccg ttcaagttct ttcaacgact ccaacaataa tttttcttgg tagggataat 3660 gactgaaggt caaaatagaa aaatcctttt gcgcacggtt ggcgcccgta tcacggcggc 3720 catgaactcg gtcaaataat ccgcttgcga atagtattgg tttccccttt atgccaagta 3780 agtggctttt gccatttagt tcccggaaaa taattaaggt cgtccaatat tggggtttcc 3840 cataaaaaat aatctgtggc atctacctgt ggaaatgttt taaaaagggc ataggtcctt 3900 tcctcataga atttccacaa gagaccgttt tcaagatacc tggcctctgg atagaggtta 3960 aaaaaactgt tcttgtcgtc gctgtaataa tccgttccgg gcaaactggg ctctccccca 4020 ctcaaggtaa ttctgacacc gtgatctttg aaccattgta accgcctttc atatttggcc 4080 ttttcttcta ggcttccccc ttgttcaatt tttggaaacc cttctatgtc gaggtattta 4140 taggctatgg catttcctcc tccataaatt cggacttcct ttactccgta ttgaagaatt 4200 ttttgctggg tcatttcaag acttgcctcc aattgggggt ccataaggag tcccaatcta 4260 aacccaatgg ccaaatcgag ctcagaacgc ttccctgcat tttcttggcc ctgtaaagat 4320 gtaccaagca ttgacaatac gatcagagtg gccaaagcct tcactttcaa catggtgttt 4380 ttatttgatt acaaacctca tctccgattt tcatcttacc ggatc 4425 // |
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