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EBI DbfetchID AF005933; SV 1; linear; genomic DNA; STD; PRO; 6168 BP. XX AC AF005933; XX DT 11-JUN-1998 (Rel. 56, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Lactobacillus casei galKETRM operon: galactokinase (galK), UDP-galactose DE 4-epimerase (galE), galactose 1-phosphate-uridyltransferase (galT) and DE putative repressor protein (galR) genes, complete cds, and mutarotase DE (galM) gene, partial cds. XX KW . XX OS Lactobacillus casei OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-6168 RX PUBMED; 9603808. RA Bettenbrock K., Alpert C.A.; RT "The gal genes for the Leloir pathway of Lactobacillus casei 64H"; RL Appl. Environ. Microbiol. 64(6):2013-2019(1998). XX RN [2] RP 1-6168 RA Bettenbrock K., Alpert C.-A.; RT ; RL Submitted (29-MAY-1997) to the EMBL/GenBank/DDBJ databases. RL AG Gentik, Universitaet Osnabrueck, Barbarastr. 11, Osnabrueck 49076, RL Germany XX FH Key Location/Qualifiers FH FT source 1..6168 FT /organism="Lactobacillus casei" FT /strain="64H" FT /mol_type="genomic DNA" FT /db_xref="taxon:1582" FT mRNA 334..>6168 FT CDS 389..1552 FT /codon_start=1 FT /transl_table=11 FT /gene="galK" FT /product="galactokinase" FT /function="kinase" FT /db_xref="GOA:O84902" FT /db_xref="InterPro:IPR000705" FT /db_xref="InterPro:IPR006203" FT /db_xref="InterPro:IPR006204" FT /db_xref="InterPro:IPR006206" FT /db_xref="InterPro:IPR013750" FT /db_xref="InterPro:IPR014721" FT /db_xref="InterPro:IPR019539" FT /db_xref="InterPro:IPR019741" FT /db_xref="InterPro:IPR020568" FT /db_xref="UniProtKB/Swiss-Prot:O84902" FT /protein_id="AAC19328.1" FT /translation="MNSTDVTKGFTEQFGKQAEHTFFAPGRINLIGEHTDYNGGHVFPC FT AISLGTYAAVGTNEDNAFRLYSANFQRLALLTSIFGSFQDKRGLWTDYFQGMARVMKTA FT GANFTHGLNVYINGNLPDGAGLSSSASLEMLVGTILNSLFDGGFEPLELVQFGVKVEND FT YIGVNSGVMDQFAIEMGRANQATLLDTNTMKYEYLPVEMGDNVIVIMNTNKRRELADSK FT YNERRSECEKALAMLQKGIEVKSLGQLSEDEFDENTYLIYDPILIKRARHAVFENQRTL FT KASKALQDGDLKTFGKLVSASGVSLAFDYEVTGIELDTLVTNALKQRGVLGARMTGAGF FT GGCAIAIVNSADVEDFIDNVGKTYREKIGYDAHFYVADIADGAKQLN" FT mRNA 1208..>6168 FT CDS 1648..2643 FT /codon_start=1 FT /transl_table=11 FT /gene="galE" FT /product="UDP-galactose 4-epimerase" FT /db_xref="GOA:O84903" FT /db_xref="InterPro:IPR001509" FT /db_xref="InterPro:IPR005886" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:O84903" FT /protein_id="AAC19329.1" FT /translation="MTIAVLGGAGYIGSHTVKQLLAAGEDVVVLDNLITGHRKAVDPRA FT RFYQGDIRDYHFLSQVFSQEKIDGIVHFAAFSIVPESMKDPLKYFDNNTGGMITLLEAM FT NQFGIKKIVFSSTAATYGEPKQVPIKETDPQVPTNPYGESKLAMEKIMHWADVAYGLKF FT VALRYFNVAGAMPDGSIGEDHHPETHIVPIILQVAAGTRTGLQIYGDDYPTKDGTNVRD FT YVHVVDLADAHILALKYLDAGNKSSAFNIGSAHGFSNLEILNAARKVTGQEIPATMGPR FT RAGDPSTLIASSEKARDILGWKPNYDDIDKIIETAWNWHENHPEGFGDRN" FT CDS 2640..4100 FT /codon_start=1 FT /transl_table=11 FT /gene="galT" FT /product="galactose 1-phosphate-uridyltransferase" FT /db_xref="GOA:O84904" FT /db_xref="InterPro:IPR000766" FT /db_xref="InterPro:IPR005849" FT /db_xref="InterPro:IPR005850" FT /db_xref="UniProtKB/Swiss-Prot:O84904" FT /protein_id="AAC19330.1" FT /translation="MTAISNHVGTIVKLNPDYTQMDAVYLTNKLLNLIGDAALDLPGDA FT DPLTNLDLMVKAAQENGKIPDSQAARQILEAQLMDLATPTPSRINQLFWDKYQAGPRVA FT TDWFFALSRANNYIQTRAIAKNVVFPAKTEYGDLEITINLSKPEKDPKDIAAAAHAAQS FT GYPACALCLQTEGYAGRTDFAARTNHRIIRFLLGGKTWGFQYSPYAYFNEHAIFLDAIH FT EPMVIDQSTFSNLLSIVSMFPTYFVGSNADLPIVGGSMLTHEHYQGGRHTFPMAKAPIE FT TQVEISGHPHVFAGIVKWPMSVIRLVSADSDELINAAEHVRQVWNQYTDETVDVRAFVD FT GKPHHTVTPIARRVGSEFQLDLVLRDNQTSAEHPDGIFHPHQDVQHIKKENIGLIEVMG FT RAILPARLKSELAEVQKYLLGEANTMKPMHKTWADQLKAKYDWTPENVEAQMQAAVGRV FT FARVLEDAGVFKRDEVGQKAFARFCRAL" FT CDS 4154..5149 FT /codon_start=1 FT /transl_table=11 FT /gene="galR" FT /product="putative repressor protein" FT /function="putative repressor of the gal-operon" FT /db_xref="GOA:O84905" FT /db_xref="InterPro:IPR000843" FT /db_xref="InterPro:IPR010982" FT /db_xref="UniProtKB/Swiss-Prot:O84905" FT /protein_id="AAC19331.1" FT /translation="MTTITDIAKAAGVSIATVSRILNYDTTLAVTPETRQRVLKTAEKL FT AYKPRRRKRVSPQTTVALIQWRSEQEELNDLYYLQIQYAIEARAASAGYHLQSLHLEQL FT AAETTQNVQGVIALGKYDAGEVATIKALAQPVVFVDQNMLAFDCDSVTSDYAGPIEQII FT HHFQSVGINDIGFLGGVETSRSGREQFQDVRTAAFDRVMHAENLMQPAFRFIGAFTPDS FT GYELMKQAITKLGDTLPHGFIVANDTMAVGALRALNEAKIEVPQRVSVISFNDVAVAKF FT TTPPLSTVHAATDQMGQTAVELLQARIKDPKRIPRQVNFKSELIMRASSL" FT CDS 5299..>6168 FT /codon_start=1 FT /transl_table=11 FT /gene="galM" FT /product="mutarotase" FT /db_xref="GOA:O84906" FT /db_xref="InterPro:IPR008183" FT /db_xref="InterPro:IPR011013" FT /db_xref="InterPro:IPR014718" FT /db_xref="InterPro:IPR015443" FT /db_xref="UniProtKB/TrEMBL:O84906" FT /protein_id="AAC19332.1" FT /translation="MDVSVEPYGQYQGHNLYELTLTNDQGMVVKLLNYGATLEKVLLPE FT ADGLHNMILSLPSREDYSKERNFLGGTVGRIVGRIRGHVWQHGDVKVDLPMNEGKNHIH FT GGDDGLDRQVYNFRIDQTATTASASFTFVDPAGHNGYPGNVKLQVTYTLENTNTLHYRL FT DAVSDEETLFNPTNHTYFAIDQPNKIDATTLTIPADDYKPLDADHLPDQGWQPVAGTVF FT DFRQGQKLGDVIHTRAAQITAERGINHPFLLKHGQTLAAKLQTDKHTMTLATTAPSVVV FT YTANHFDGT" XX SQ Sequence 6168 BP; 1656 A; 1388 C; 1564 G; 1560 T; 0 other; tctcaggcag ataatgacct aaattgtaag ttggcacaac gatggttaaa tctaagttcg 60 acataattgc ctccttgaag ctcgttttga tcattatcgt caaattacgg gtgacgtcaa 120 tcaaaatgat tcatgtatga agttgggttg ttgacatggg gaatgctaac tatcggcgcc 180 atgtgggaag ggacattatg aacctgctag aatttttagt aaaaggaagt tgctttaaat 240 tcagcgattt ttaaatgacc ggtaaaacaa aaaacaaaaa aatttggcta cagcgcttcc 300 aatccgtcat tatttttagt aaaataaaaa cggttacaaa aatcagtagc ggataaataa 360 gcttaacttc aattaggagg tattgacggt gaatagtaca gatgtcacga aaggttttac 420 ggaacaattt ggtaagcagg cggagcacac tttctttgca ccaggacgca ttaatttaat 480 tggtgaacac accgattaca atggtggcca cgttttccca tgcgccattt cgttgggcac 540 ttatgcagcg gttggcacca atgaggacaa cgcttttcgg ttatactcgg ccaacttcca 600 aaggttggca ttattgacat ccattttcgg atctttccaa gataagcgcg gcttgtggac 660 agattacttc caaggtatgg cgcgggtgat gaagacggcg ggtgcgaact tcacgcatgg 720 cctcaatgtc tacatcaacg gcaacttacc tgatggcgct ggattatcaa gttctgcttc 780 tctggaaatg ctggtcggaa cgattctgaa cagtcttttt gatggcggct ttgaaccgtt 840 ggagctggtt cagttcggcg ttaaagtcga aaacgactat atcggcgtga actccggtgt 900 gatggatcag ttcgcgattg aaatgggtcg cgcaaatcaa gcaacgctgt tggatacaaa 960 cacgatgaaa tacgagtacc tgccagttga gatgggcgat aacgtgattg tcatcatgaa 1020 taccaacaag cgtcgtgagt tggctgattc caagtacaac gaacgccgca gtgaatgcga 1080 aaaagcactg gcaatgctac aaaaaggcat tgaagtcaaa agtctgggcc aactgagtga 1140 agacgaattc gacgagaata cttatctcat ttacgatcca atcctgatca aacgcgcgcg 1200 ccacgccgtc tttgaaaatc agcggacatt gaaggcttcg aaagcactgc aagatggcga 1260 tttgaagacc tttggcaaac tagtctctgc ttctggagtt tcgttggctt ttgactacga 1320 agtcactggc atcgagcttg atacccttgt gaccaatgcc ttgaagcaac ggggtgtgct 1380 cggtgctcgc atgaccggtg ctggctttgg cggttgcgcc attgcgattg tcaacagtgc 1440 tgatgttgaa gactttattg acaacgttgg caagacttat cgtgaaaaga tcggctatga 1500 cgcccatttc tatgtggccg atattgccga tggtgctaag caactgaact aactctgcta 1560 aaatagaaaa agtgttataa agtagagcgt gagccagacc gatttctagt ctgacgaaca 1620 cgttttaaaa aagggagata ttttattatg acaattgcag ttttaggcgg cgctggttac 1680 atcggttcac atacggtaaa acaattactc gctgccggcg aggatgttgt ggttttggat 1740 aacttgatta ctgggcatcg taaggcggtt gatccacgcg cccgttttta tcaaggcgac 1800 atccgcgact atcattttct gagccaagtg ttcagccagg aaaagattga cgggattgtc 1860 cattttgcgg cgttctcaat tgtgccggaa tccatgaaag atccgttgaa gtattttgat 1920 aacaacactg gcggcatgat caccttgctt gaagccatga atcaattcgg catcaagaaa 1980 attgtcttct catctacggc agcaacttat ggcgaaccta agcaggtccc aattaaagaa 2040 actgatccgc aagtaccgac caacccttac ggtgaaagca agttggccat ggaaaagatc 2100 atgcattggg ctgatgtggc ttatgggctg aagttcgtgg ctttgcgtta cttcaatgtt 2160 gcgggtgcga tgccagacgg ttccatcggt gaagaccatc atccagaaac gcatattgtg 2220 ccaattatct tgcaggttgc agcaggtacg cgaactggtt tgcaaattta tggtgacgac 2280 tatccaacta aggatggtac caatgtgcgt gactatgttc atgtggtcga tcttgctgat 2340 gcccacattt tggccttgaa gtatctggat gccggcaata agtcatccgc gttcaacatt 2400 ggttctgcgc atggtttctc caaccttgaa attctaaatg cagcgcgtaa ggtgactggc 2460 caagagattc cagccacgat gggcccgcgg cgtgccggtg atccaagcac tttgattgct 2520 agctcagaaa aggctcgtga cattttaggc tggaagccaa actatgacga tattgacaag 2580 attatcgaaa ccgcatggaa ctggcacgaa aatcacccag aaggatttgg tgaccggaat 2640 tgaccgcgat tagcaatcac gttggtacca ttgtgaaact caatcccgat tacacccaga 2700 tggatgcagt ctatctgacc aacaaattgt tgaatttgat cggagatgct gcccttgacc 2760 tgcctggtga tgctgatcca ttgacgaacc ttgatctgat ggtgaaagct gcgcaggaga 2820 atggtaaaat tccagattca caggcagcac gtcagatttt ggaagcgcag ctgatggacc 2880 ttgctacccc gacaccaagt cggatcaatc aactgttttg ggataagtat caggcgggac 2940 cacgggttgc aacagactgg ttctttgctt tgagtcgcgc gaataattat attcaaacgc 3000 gagcgattgc taagaacgtt gtttttccag ctaaaactga atatggcgac ctcgaaatca 3060 ccatcaacct gtctaaaccg gaaaaagatc cgaaagacat tgctgctgcg gcacatgccg 3120 cccagtctgg ctatccggcg tgtgccttgt gtttacaaac ggaagggtat gccggtcgaa 3180 ctgactttgc ggcgcgaacc aatcatcgca ttattcgctt tttgcttggc ggtaaaacat 3240 ggggattcca atattcgcca tatgcttact tcaacgagca cgcgattttt ctggatgcta 3300 tccatgaacc catggtgata gaccaaagta ccttcagcaa cttgttgtcc atcgtttcaa 3360 tgtttccaac ttattttgtt ggctcaaatg ctgacttgcc aattgtcggc ggttcgatgc 3420 tgacgcatga acattaccaa ggcggtcgcc atacatttcc aatggccaaa gcaccaattg 3480 agacgcaggt tgaaattagc gggcatccgc atgttttcgc tggcatcgtc aagtggccga 3540 tgagtgttat tcgcttagtg agtgctgatt cggacgagtt gataaatgca gccgaacatg 3600 ttcggcaggt ttggaatcag tataccgatg aaacagttga cgtccgcgca tttgttgatg 3660 gcaaaccgca tcatacggtg accccaattg ctcggcgcgt tggttcggaa ttccagctgg 3720 atttagtgct gcgcgacaat caaactagtg cagagcatcc ggatggtatt ttccatccgc 3780 atcaagacgt gcaacatatc aagaaagaaa acattggctt gatcgaagtg atgggacgtg 3840 ccattttacc ggcacgatta aagtcggagt tagctgaagt ccagaagtat ttactgggtg 3900 aagcaaacac gatgaaaccg atgcacaaaa catgggccga tcagttgaaa gctaaatatg 3960 attggacccc ggaaaacgtc gaggcacaaa tgcaggcagc ggttggtcgt gtgtttgctc 4020 gggtcttaga agatgctggt gtgttcaaac gcgatgaagt tggtcaaaaa gcatttgctc 4080 gcttctgtcg ggcactgtag caacaggcta tgttgctgaa gccggccagg agtcaatgga 4140 aacggagaaa acgatgacca cgattacaga tattgcgaag gcggcagggg tttcaattgc 4200 gactgtctcg cgtattttaa attatgatac gacgttggct gtcacgcccg aaacgcgcca 4260 acgcgtgttg aaaacagccg aaaagctggc gtacaaacca cgtcgacgta aacgtgtgtc 4320 cccgcaaacc accgttgcct tgattcagtg gcgcagcgag caagaagagc tgaatgatct 4380 gtattattta cagattcaat atgctattga ggcacgggcg gcctcagctg gctatcattt 4440 gcagagcttg catttggagc agttggcagc tgaaacgaca caaaacgtcc aaggtgtgat 4500 tgcgttgggt aagtacgacg ctggcgaagt ggcaacgatc aaagccttgg cgcaaccagt 4560 tgttttcgtg gatcaaaata tgttggcatt tgactgcgac tcggtcacca gcgattatgc 4620 cggtcctatc gagcaaatta ttcatcattt tcaatcggta gggatcaacg acatcgggtt 4680 tctcggtggc gttgaaacga gtcgcagcgg acgcgagcag tttcaggacg tgcggacagc 4740 cgcatttgac cgggtgatgc atgcagaaaa cttgatgcag ccagcgtttc gttttattgg 4800 cgcgttcacg cctgattcag gttacgaact gatgaaacaa gcgatcacta aactcggtga 4860 caccttaccg cacggtttca ttgtggccaa cgacacgatg gcagtcggtg cgttgcgcgc 4920 tttgaacgag gctaagatcg aggtgccgca acgcgtctct gtgatcagct tcaacgatgt 4980 ggcggtcgcc aagttcacga ccccgccact ttctacggtg catgctgcca ctgaccaaat 5040 gggccaaacc gcggtcgaac tattgcaggc acgcatcaaa gatccgaaac gcataccacg 5100 tcaggttaac ttcaaaagtg agcttattat gcgagccagc agcttgtagg aacggtatta 5160 taacgtgttc ccccgcgaag aaacctgtcc ataatgacct caagtgcaat gaccaaaagc 5220 gggtcatcat acttgaggtc ggctgatgct ttggtttcta accgcgctgg cttacgctcg 5280 tttataaagg aggacaagat ggacgtttca gttgaaccat acggtcagta tcaaggccac 5340 aatttgtatg agctgacctt gaccaatgat caaggcatgg ttgtgaagtt gttgaattat 5400 ggtgcaacct tggaaaaagt gttgttacca gaagctgatg gcttgcacaa catgattctg 5460 tcgctgccaa gtcgggagga ttactcgaaa gagcgcaact tccttggtgg cacagtgggt 5520 cgaattgttg gccgtattcg tggtcacgtt tggcagcatg gcgatgtgaa agttgatctg 5580 ccaatgaacg aaggcaagaa tcacattcac ggcggggatg acggtctgga tcggcaagtt 5640 tataacttcc gtattgacca gacagctacc acagctagtg ctagttttac ctttgttgat 5700 ccagcagggc ataatggcta tccgggcaac gtcaagctgc aagtgactta taccttggag 5760 aatacgaata ccttgcacta tcgtctggat gcagttagtg atgaagaaac gctcttcaat 5820 ccaactaacc atacctattt tgcgattgac cagccaaaca aaattgatgc caccacgctg 5880 acgattccgg ccgacgatta caaaccgctt gatgcagacc atctgccaga tcaaggctgg 5940 cagccagttg ccggaacggt ctttgatttt cggcaagggc aaaagcttgg cgatgtcatt 6000 cacactcgag cagcgcaaat cactgcagaa cggggcatta atcacccatt cctactgaaa 6060 cacggtcaaa ccctcgcggc caaactccag actgacaaac atactatgac cctggcaacc 6120 actgcaccgt ccgtcgtcgt ttacaccgcc aaccactttg acggtacc 6168 // |
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