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EBI DbfetchID AF005098; SV 1; linear; genomic DNA; STD; PRO; 5565 BP. XX AC AF005098; XX DT 11-SEP-1997 (Rel. 52, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Lactococcus lactis RNAseH II (rnhB) gene, partial cds, positive regulator DE GadR (gadR), GadC (gadC) and glutamate decarboxylase (gadB) genes, complete DE cds. XX KW . XX OS Lactococcus lactis OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus. XX RN [1] RP 1-5565 RX DOI; 10.1046/j.1365-2958.1998.00676.x RX PUBMED; 9484886. RA Sanders J.W., Leenhouts K., Burghoorn J., Brands J.R., Venema G., Kok J.; RT "A chloride-inducible acid resistance mechanism in Lactococcus lactis and RT its regulation"; RL Mol. Microbiol. 27(2):299-310(1998). XX RN [2] RP 1878-2423 RX DOI; 10.1007/s004380050697 RX PUBMED; 9604892. RA Sanders J.W., Venema G., Kok J., Leenhouts K.; RT "Identification of a sodium chloride-regulated promoter in Lactococcus RT lactis by single-copy chromosomal fusion with a reporter gene"; RL Mol. Gen. Genet. 257(6):681-685(1998). XX RN [3] RP 1-5565 RA Sanders J.W., Venema G., Kok J., Leenhouts K.; RT ; RL Submitted (20-MAY-1997) to the EMBL/GenBank/DDBJ databases. RL Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, RL University of Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands XX FH Key Location/Qualifiers FH FT source 1..5565 FT /organism="Lactococcus lactis" FT /map="between ldh and leu-ilv" FT /strain="MG1363" FT /mol_type="genomic DNA" FT /clone="NS3" FT /db_xref="taxon:1358" FT CDS <1..503 FT /codon_start=3 FT /transl_table=11 FT /gene="rnhB" FT /product="RNAseH II" FT /function="specifically degrades the ribonucleotide moiety FT on RNA-DNA hybrids" FT /EC_number="3.1.26.4" FT /note="RnhB; similar to Escherichia coli RNAseH II" FT /db_xref="GOA:O30415" FT /db_xref="InterPro:IPR001352" FT /db_xref="UniProtKB/Swiss-Prot:O30415" FT /protein_id="AAC46185.1" FT /translation="AVILPKNCKIRGLNDSKKVPKSKHHAILSEIQEKALAIGVGIVDA FT EKIDEVNIYEATKIAMIQAVSKLSLKPEHLLIDAMVLDLPIAQTKIIHGDARSASIAAA FT SIVAKVTRDEMMKDFALEFPEYDFEHNAGYGTAKHLAALTKYGITRIHRKSYEPIKSMV FT NFK" FT terminator 526..555 FT /gene="rnhB" FT -35_signal 941..946 FT /gene="gadR" FT -10_signal 965..969 FT /gene="gadR" FT mRNA 979..1966 FT /gene="gadR" FT RBS 1084..1089 FT /gene="gadR" FT CDS 1095..1925 FT /codon_start=1 FT /transl_table=11 FT /gene="gadR" FT /product="positive regulator GadR" FT /function="activates chloride dependent transcription of FT gadCB" FT /db_xref="GOA:O30416" FT /db_xref="InterPro:IPR001387" FT /db_xref="UniProtKB/TrEMBL:O30416" FT /protein_id="AAC46186.1" FT /translation="MYKKYGDCFKKLRNQKNLGLSYFSKLGIDRSNISRFEHGKCMMSF FT ERIDLMLEEMQVPLSEYELIVNNFMPNFQEFFILELEKAEFSQNRDKIKELYSEVKETG FT NHLLTVTVKTKLGNISQTEVKEIEAYLCNIEEWGYFELTLFYFVSDYLNVNQLELLLFN FT FDKRCENYCRVLKYRRRLLQIAYKSVAIYAAKGERKKAENILEMTKKYRTVGVDLYSEV FT LRHLARAIIIFNFENAEIGEEKINYALEILEEFGGKKIKEFYQNKMEKYLKRSI" FT stem_loop 1926..1967 FT /gene="gadR" FT /note="functions as terminator; may function in chloride FT dependent transcription" FT -10_signal 1987..1992 FT /gene="gadC" FT mRNA 2001..5037 FT RBS 2058..2065 FT /gene="gadC" FT CDS 2069..3580 FT /codon_start=1 FT /transl_table=11 FT /gene="gadC" FT /product="GadC" FT /note="Putative glutamate - gamma-aminobutyrate antiporter; FT Functions in glutamate dependent acid stress resistance. FT Expression induced by chloride and at low pH. Homologue of FT Shigella flexneri GadC" FT /db_xref="GOA:O30417" FT /db_xref="InterPro:IPR004841" FT /db_xref="UniProtKB/Swiss-Prot:O30417" FT /protein_id="AAC46187.1" FT /translation="MNQKKLSLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLL FT WFLPVALCAAEMATVEGWKNGGIFSWVSQTLGERFGFAAIFFQWFQITVGFVTMIYFIL FT GALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVIPSVI FT LFGLAAAYFIGGNPIEIPINSHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELEN FT PKRNYPLAMILLVILAISLDAIGGFSVAAVIPQKELSLSAGVIQTFQTLILHFNHHLGW FT LVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLPKFLRKTNTHEVPVPLVMIQGI FT IVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIVYFVLIYKKQNLKRTYNVP FT GKIIGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIIYE FT LHDKKGHDTIEEPTHFKAGDVNPAIYPAARGEHHIIKKEEHILKH" FT RBS 3587..3591 FT /gene="gadB" FT CDS 3600..5000 FT /codon_start=1 FT /transl_table=11 FT /gene="gadB" FT /product="glutamate decarboxylase" FT /function="glutamate dependent acid stress resistance" FT /note="expression induced by chloride and at low pH; FT similar to Synechocystes sp. GadB" FT /db_xref="GOA:O30418" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:O30418" FT /protein_id="AAC46188.1" FT /translation="MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQL FT VQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMI FT ADLWNASEKGKIYGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVISSGY FT QVCWEKFCVYWDIEMREVPMDREHMSINLEKVMDYVDEYTIGVVGIMGITYTGRYDDIK FT ALDNLIEEYNKQTDYKVYIHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLV FT YPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSHSASQLIGQYYNFVRYGFDGY FT KAIHERTHKVAMYLAEEIEKTGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLM FT KGWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIDALNKAHILFHQEPE FT NKTYGFTH" FT terminator 5003..5037 FT /gene="gadB" FT CDS complement(5075..>5565) FT /codon_start=3 FT /transl_table=11 FT /product="unknown" FT /db_xref="GOA:O30419" FT /db_xref="InterPro:IPR006685" FT /db_xref="UniProtKB/TrEMBL:O30419" FT /protein_id="AAC46189.1" FT /translation="IPVANVLAGAGILGVALGFAGRDLVADIINGFFIIVEHQINVADT FT VAFAGLDIEGVVKTVGIRSITVIGADGATTFIPNRNIAALKNYSYKERTVNLDVPVTDE FT NISEKKAQIIEVNANYPQVKYLGIINIEDKLFLRTSLTAPLSKITPLKMEILDKYYEK" XX SQ Sequence 5565 BP; 1869 A; 771 C; 1073 G; 1852 T; 0 other; ctgcagtaat tttgccaaag aattgtaaaa ttcgtggttt gaatgatagt aaaaaagtgc 60 caaaatcaaa gcatcatgct attctatctg aaattcaaga aaaagcgcta gcgattggag 120 ttggaattgt tgatgccgaa aaaattgatg aagtaaatat ttatgaggcg acaaaaattg 180 caatgattca ggcagtatca aaattatctc ttaaacctga acatctctta atagatgcga 240 tggttttaga tttgcccatt gctcagacga aaatcattca tggagatgct cgttcagctt 300 caattgcggc cgcatcaatt gtagctaaag tgactcgtga tgaaatgatg aaggatttcg 360 ctttagaatt tccagaatat gattttgaac ataatgcagg ctatggaaca gcaaaacatc 420 ttgcagctct gacaaaatat ggtatcacaa gaattcatcg gaaatcctat gaaccaatta 480 aatcgatggt caatttcaaa tagtagatta tgtaagtaaa aaaaggaaaa cgtgaacgat 540 atttggtcac gtttttttgc tgacaagtct gtcagtaatt attttcaaag gtttcaaaaa 600 tatagtctag taatttgcta gactaatctt ctgtttttta ataataaact aattttttgt 660 taatctaaat gacaaaatta ataagcagag ttttttataa aattagctac ttataaaaaa 720 tttgaaattg gtatagttaa atctgttata atttccaata ttttttaata ataattattt 780 taacaaaata cttatatcaa aactctttca aagtatataa tgagcgttgt ataagctttt 840 atgtctttct atatcaactt ttaatagaaa tataaagtaa tataaatgtt tttataataa 900 attatgtgag atatattttt ttgtccgtac tggtatagat ttgacgatta agtcttaaat 960 aagttataat ctcaattgcg taatttctta aatacagaaa taacaactac attggtagac 1020 tgattaaaaa gtgtacttga tgaactgtta taaaccttaa aaaaataaaa ataatagttt 1080 gggggatgtt aaagatgtat aaaaaatatg gagattgttt taaaaagttg cgaaaccaaa 1140 agaatttagg gttatcatac tttagtaaac ttggaataga ccgttcaaat atatctagat 1200 ttgaacatgg aaaatgtatg atgagttttg agcgtataga tttgatgtta gaagaaatgc 1260 aagttccgtt atctgagtac gaattgattg taaataattt tatgccgaat ttccaagaat 1320 tttttatatt agaattggaa aaagctgaat ttagccaaaa tcgagataaa ataaaagagt 1380 tgtattctga ggtcaaagaa acggggaatc atttactgac ggttaccgtg aaaacgaagc 1440 ttgggaatat aagtcagaca gaagttaaag aaattgaagc ttatctttgc aatattgaag 1500 agtggggata ttttgaactt actttatttt attttgtatc tgattatctc aatgtcaatc 1560 aattagaatt gctgcttttt aattttgata aaagatgtga aaattactgt agagtcttaa 1620 aatatagaag gagactattg caaatagcct ataaaagtgt tgcgatatac gcggctaaag 1680 gagaaagaaa aaaagccgaa aatattttag aaatgactaa aaaatatcga actgtgggag 1740 tcgatttata ttcagaagta ttaagacatc ttgctagagc tatcattatt tttaattttg 1800 aaaatgcaga gattggggaa gaaaaaataa attatgctct tgagattttg gaagaatttg 1860 gaggaaagaa gataaaagaa ttctatcaga ataaaatgga aaagtatttg aaaaggtcaa 1920 tttagtctct tttgagctgt tgctttaaag caacagctca aaagagattt tctttattct 1980 agagcatata ctagagggtg aagataggtt gtctgaagca ttataacttg tcttttaaaa 2040 aattcaatca taaatataag gaggtatgat gaatcaaaaa aaattatcat tattcggttt 2100 tttcgcatta accgcttcaa tggttttgac tgtctatgag tatccgactt ttgccacgtc 2160 aaaattacat ttggtgttct ttttacttct cggaggacta ctatggtttt tgcctgtagc 2220 gctctgcgca gcagaaatgg cgacggttga aggctggaaa aatggtggaa tctttagttg 2280 ggtcagtcaa actttaggtg agcgctttgg ttttgcagcc atattttttc agtggttcca 2340 aattacagta ggttttgtca ctatgatcta tttcatttta ggggccctct cttatgtgtt 2400 aaattttcag gcgctcaata cagatccatt gataaaattt attggtttac taatcatttt 2460 ttggggattg actttttctc aattaggtgg gacgcaacgg actgccaaat tagtaaaagc 2520 tggctttgta gttggaatag tgattccatc ggttatcttg tttggattag cagcggcata 2580 ctttatcgga ggcaatccta tagaaatacc aattaacagc catgcttttg taccagattt 2640 ttcacaggta tcaactttag tagtttttgt ttcttttatt ctggcttata tgggggtaga 2700 agcctcagct tcacatatta atgaacttga aaatccaaaa cgaaattatc ccttagcaat 2760 gattttatta gtaattttgg ctatttcttt agatgccata ggtggatttt ctgtagcagc 2820 agttattcct caaaaagagt tatcattaag tgcaggggta atccaaactt ttcaaacgtt 2880 aatcttacat tttaatcatc atttgggatg gttagttaaa gtgattgcac taatgattgc 2940 ctttggggtt atgggagaag tgagttcatg ggttgttggt ccttctagag ggatgtttgc 3000 agcagcacaa agaggtttat taccaaaatt tttacgtaaa acgaatacac atgaagtccc 3060 tgttccttta gttatgattc aaggaatcat tgttacactt tggggcgctg tattaacttt 3120 tggaggagga ggaaataatt tatctttctt agttgccatt tcactgactg tagtgattta 3180 tttggtgggt tacctcttgt tctttattgt ttactttgtt ttaatctata aaaaacaaaa 3240 tttaaagcgt acttataatg ttccaggtaa aataatagga aaaacaatca ttgcaggaat 3300 tggattctta ttatcaattt ttgctctatt tatttccttt gttcctccag catcaattgc 3360 gaaaaatgaa actcacacct atcaaatgat acttcttata agttttgttg tgaccgctat 3420 cttgccattt attatttatg aattgcatga taaaaaggga catgatacta ttgaagaacc 3480 aacacacttt aaagcaggag atgtgaaccc tgcgatttat ccagcagctc gtggagagca 3540 tcatattatt aaaaaagaag aacatatctt aaaacattga aaaattggag gatgtacata 3600 tgttatacgg aaaagaaaat cgagatgaag cggagttctt ggaaccaatt tttggttcag 3660 aaagtgaaca agtggattta cctaaatata aattagctca acaatcaatt gagcctcgag 3720 tggcctatca gttagttcaa gatgaaatgc tagatgaagg gaacgctcgt ttaaatttgg 3780 ccacattctg tcaaacttat atggaacctg aagcagtcaa gctgatgagt cagaccttgg 3840 aaaaaaatgc gattgacaaa tcagaatatc caagaacaac tgaaattgaa aaccgttgcg 3900 tcaacatgat cgctgacctt tggaatgcga gtgaaaaagg aaaaatttat gggacttcga 3960 caattggttc ttcagaagct tgtatgcttg ggggaatggc tatgaagttt tcttggcgta 4020 agcgagcaga aaaattaggc ctagatatta atgcgaaaaa gccaaactta gtcatttcct 4080 ctggttatca agtttgctgg gaaaaattct gtgtttattg ggatattgaa atgagagaag 4140 tgccaatgga tagagaacat atgtcaatca atttggaaaa agtgatggat tatgttgatg 4200 aatatacgat tggagtagtt ggaattatgg ggattactta tactggtcgt tatgatgata 4260 tcaaagcttt ggataatttg attgaagaat ataataaaca gacagactac aaagtttata 4320 ttcacgtaga tgctgcttca ggaggacttt atgctccttt tgttgagcca gaacttgagt 4380 gggatttccg tttgaaaaat gtcatttcaa tcaatacttc aggacataaa tatggtttag 4440 tatatcctgg tgtaggttgg gtcttgtggc gtgacaaaaa atatttacct gaagagttaa 4500 tttttaaagt aagttatctt ggaggagaat taccaacaat ggcgattaat ttttctcaca 4560 gtgcttctca attaatcggt caatactata attttgtacg ttatggattt gatggatata 4620 aagctattca tgagagaacg cataaagtag ccatgtattt agcagaagaa attgaaaaaa 4680 caggaatgtt tgagattatg aacgatgggg cacaattacc aattgtctgc tacaaattaa 4740 aagaaaattc aaaccgtggt tggaatcttt atgatttggc agatcgttta ttaatgaagg 4800 gatggcaagt gcctgcttat ccacttccta aaaatttgga aaatgaaatc attcaacgtt 4860 tagtaattcg agcagatttc gggatgaata tggcatttaa ctatgttcaa gatatgcaag 4920 aagcaattga tgcactaaat aaggctcata ttctatttca tcaggaacct gaaaataaaa 4980 catatggctt tactcactaa agataaaagc gatatatcta agatatatcg cttttatttt 5040 gttttaggct atttactaat tagcttgtcg cttattattt ttcatagtat ttatccaaaa 5100 tttccatttt taaaggagta attttagata ggggggcagt tagacttgtt cttaggaaga 5160 gcttatcttc aatgttgatg atacccagat atttaacttg agggtagtta gcattgactt 5220 ctataatttg ggcttttttc tcactaatat tttcgtctgt cacgggcaca tctaggttga 5280 ccgttctttc tttataagag taatttttga gagcagcaat atttcggttt ggaataaaag 5340 tagtcgcacc gtcggctccg ataacagtaa tggaacgaat tcctacggtt ttcaccactc 5400 cctcaatatc aagaccggca aaggcaaccg tatccgcaac attgatttga tgttcaacga 5460 taataaagaa accattaatg atatcagcga ctaaatctcg tccagcgaaa ccaagagcga 5520 ctccaaggat acccgcgcca gctaggacat ttgcaacagg aattc 5565 // |
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