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EBI DbfetchID AF003948; SV 1; linear; genomic DNA; STD; PRO; 7240 BP. XX AC AF003948; XX DT 13-MAR-1998 (Rel. 55, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Rhodococcus opacus chloromuconate cycloisomerase (clcB), ClcR (clcR), DE chlorocatechol 1,2-dioxygenase (clcA), dienelactone hydrolase (clcD) and DE putative transposase homolog genes, complete cds. XX KW . XX OS Rhodococcus opacus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. XX RN [1] RP 1-7240 RX PUBMED; 9495745. RA Eulberg D., Kourbatova E.M., Golovleva L.A., Schlomann M.; RT "Evolutionary relationship between chlorocatechol catabolic enzymes from RT Rhodococcus opacus 1CP and their counterparts in proteobacteria: sequence RT divergence and functional convergence"; RL J. Bacteriol. 180(5):1082-1094(1998). XX RN [2] RP 1-7240 RA Eulberg D., Schloemann M.; RT ; RL Submitted (13-MAY-1997) to the EMBL/GenBank/DDBJ databases. RL Institute for Microbiology, University of Stuttgart, Allmandring 31, RL Stuttgart 70569, Germany XX FH Key Location/Qualifiers FH FT source 1..7240 FT /organism="Rhodococcus opacus" FT /strain="1CP" FT /mol_type="genomic DNA" FT /db_xref="taxon:37919" FT CDS complement(579..1700) FT /codon_start=1 FT /transl_table=11 FT /gene="clcB" FT /product="chloromuconate cycloisomerase" FT /note="ClcB" FT /db_xref="GOA:O67985" FT /db_xref="HSSP:1NU5" FT /db_xref="InterPro:IPR013342" FT /db_xref="UniProtKB/TrEMBL:O67985" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC38249.1" FT /translation="MPNLTVSGVRTTIVDLPILRPHRFANHSIDAQTYLLVEVVTDAGF FT VGLGEGVSPGGPWWGGESIEGQKQIIDCYLAPALIGSDVEDLAGTRRQLDRIAFANLFA FT KAALETALLDAIGQAHNVALSTLLGGATRTELPLRWPLSGAGTGGTLEEAAERVAAGYR FT RIKFKMGALAPREDIDRVGELVDKIGDGVDYLGDPNGTWDLRTATWAVRELEAIGLSAV FT EQPVERRDVRGLAELRSRADRIDIMADESVCVPADALEVVRERACDSVAVKPGKAGGLR FT AAALIAAILDTASIGPYGGTALESPVGTAASAHLFATFPRLLHGCELVGPLLLADSVIT FT EPLAYTDGILTVPTGPGLGVRLDHDKVDRYRRR" FT CDS 2509..3420 FT /codon_start=1 FT /transl_table=11 FT /gene="clcR" FT /product="ClcR" FT /note="similar to LysR-type regulators involved in FT catabolism of chloroaromatic compounds" FT /db_xref="GOA:O67986" FT /db_xref="HSSP:1IXC" FT /db_xref="InterPro:IPR005119" FT /db_xref="UniProtKB/TrEMBL:O67986" FT /protein_id="AAC38250.1" FT /translation="MIPLDVRQLECFIAVAEERHVGHAAARLHMTQPPLTRRISRLERE FT IGAQLFVRTLTGVELTAAGLVLLDRAYRIVALAENTLERVRLADAGEAGALAVGYFGST FT IFGLVPRLLRLFLDTRPEVRIVIERATRDMQAQAIRDGRMHIGFSRQYPAEPDLAVRNI FT ASEPLYVALPETHPLLSKQKVLLADIQNEPLVLFPSAPRPGFADEVVQLLMRTGGEPRI FT EAEASDVVTALAYVAIAQLCAVVPRSASNIALPGVVYVPLADAPPESLSCIYRAIETPT FT LVRAFLDHLDLLDPATLDLYED" FT stem_loop 3583..3617 FT CDS 3643..4416 FT /codon_start=1 FT /transl_table=11 FT /gene="clcA" FT /product="chlorocatechol 1,2-dioxygenase" FT /note="ClcA" FT /db_xref="GOA:O67987" FT /db_xref="InterPro:IPR015889" FT /db_xref="PDB:1S9A" FT /db_xref="UniProtKB/Swiss-Prot:O67987" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC38251.1" FT /translation="MANTRVIELFDEFTDLIRDFIVRHEITTPEYETIMQYMISVGEAG FT EWPLWLDAFFETTVDSVSYGKGNWTSSAIQGPFFKEGAPLLTGKPATLPMRADEPGDRM FT RFTGSVRDTSGTPITGAVIDVWHSTNDGNYSFFSPALPDQYLLRGRVVPAEDGSIEFHS FT IRPVPYEIPKAGPTGQLMNSYLGRHSWRPAHIHIRITADGYRPLITQLYFEGDPYLDSD FT SCSAVKSELVLPVNKIDIDGETWQLVDFNFILQHN" FT CDS 4429..5187 FT /codon_start=1 FT /transl_table=11 FT /gene="clcD" FT /product="dienelactone hydrolase" FT /note="ClcD" FT /db_xref="GOA:O67988" FT /db_xref="InterPro:IPR002925" FT /db_xref="UniProtKB/Swiss-Prot:O67988" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC38252.1" FT /translation="MCHNKSSAPPTPAHISIQQNRTPGTDPVPYLHASSDTGSGDTIVL FT LTDIFGVTPFYRHLAAMLAEKGHDVVIPDVFHRVGHATDPGRDAALARRRQLDDRLAIE FT DIERTVAHTVDDQQTFGVLGFCLGGSFALLTAAAHPNQVTATYYAFPKGAPGAKVPVKP FT PLEAADAIDGPVLCHWGRDDYIDHEEIDQLEQILASAPGPSEIRWYDNAGHSFLAGLTE FT PNHPSTAAAHDSWQRTVEFFERYLTVGSAN" FT CDS 5628..6356 FT /codon_start=1 FT /transl_table=11 FT /product="putative transposase homolog" FT /note="ORF5" FT /db_xref="GOA:O67989" FT /db_xref="InterPro:IPR002559" FT /db_xref="UniProtKB/TrEMBL:O67989" FT /protein_id="AAC38253.1" FT /translation="MGSRKQHRKPKPYPSDLTDAQWELIAPMVPVSSAGRPPIHSRRRI FT VDAILYVNRTGCSWRQLPHDFPKWETVYWYFKVWNESGVTDRIHDALRARARDRGGRDP FT MASAGMVDAQAVKGADTVGTDTRGWDGAKRVNGRKRHLVTDTLGLLVVVLVTAGSVQDR FT DGGRRVLARAKTAMPSMVVVWADGGYAGKLVAWVDTLPHRSGYRSETQRAEWVCCPTAP FT LGDRTDAVVAGALSPPRPRL" XX SQ Sequence 7240 BP; 1323 A; 2464 C; 2168 G; 1285 T; 0 other; gaattcaatg ccatcccgat cgagaaatac gccgtcacgc gagcggtcaa cgacctactg 60 cgctgctcgg tccgcccgca ttccgcgatc acctcatcga ccagggacgc gggaaacacc 120 ctggtcaaca accccaccga caccacatcc gacaaccgcc gatccgagac cggtttcacc 180 catccgctcc gaggcacacc tcatcgtaac gcaacccgca ctaactgaac ggtattgcac 240 ctaaacgacc acagcactgc ctgcggccag ttcatcgtgt acgtatcgtc tgccgccggg 300 cgactgtggt gtccggtgca cggcgtagcc catgaaccgt cgggtcgatc ggctccgcac 360 agcaaatact gcactttagc gctcccggaa ggtgcaaggt gatgggagca cgggctccca 420 tgctgcaagt tgcgctgttc gtcggggctc gtcaggatca attcgactac gtcgtccgcg 480 atcttgttct tctcggagac gacaagttcg cggcgggtgc cgggtttgtg catggatacg 540 taacctgtca cgagtggaag gttgggcggc gtaggccgtc agcgtctgcg gtagcggtcg 600 accttgtcgt ggtccagccg cacccccaga ccgggacccg ttggaaccgt gagtattccg 660 tcagtatagg cgagcggctc ggtgatcacc gagtcggcca gcagcaacgg gcccaccagc 720 tcgcagccgt ggagcagcct cgggaatgtg gcgaacaggt gcgcggaggc cgccgtaccg 780 accgggcttt ccagcgccgt accgccgtaa ggaccgatcg aagcggtgtc gagtatggcc 840 gcgatcaacg ccgcagcccg caagcctccg gcctttccgg gtttgaccgc gaccgagtcg 900 caggctcgtt cgcgtacgac ctcgagcgcg tctgcaggca cgcataccga ctcgtcggcc 960 atgatgtcga tgcgatcggc tcgcgaccgt agttcggcaa gcccgcgcac gtcgcggcgc 1020 tcgaccggct gctcgaccgc gctgaggccg atggcctcga gttctcgcac ggcccaggtc 1080 gcagtccgca gatcccacgt gccgttgggg tcgccaaggt agtcgacgcc gtcgccgatc 1140 ttgtcgacca gttccccgac gcggtcgata tcctcgcgcg gggcaagcgc ccccatcttg 1200 aacttgatcc tgcggtagcc agccgccact cgttccgcag cctcttcgag tgtccccccg 1260 gtcccggcgc cgctgagcgg ccagcgcagc ggcagttcgg tacgggtggc accgcctagc 1320 agggtcgaca gggcaacgtt gtgtgcctgc ccgatcgcat cgagcaacgc ggtctcgagc 1380 gcggccttgg cgaacaggtt ggcgaacgca atccggtcta gttgtcgacg ggtcccggcg 1440 aggtcttcga cgtcgctgcc gatcagtgcc ggggcaaggt agcagtcgat gatctgcttt 1500 tgcccttcga tactttctcc gccccaccac ggtccgcctg gtgatacccc ttcgccgagg 1560 ccgacgaatc cggcgtctgt gacgacctcg acgaggaggt aagtttgtgc gtcgatgctg 1620 tggttcgcga atcggtgtgg gcgcaggatc ggtaggtcga cgatcgtggt ccgcacaccg 1680 ctgacggtga ggttcggcat ggccgctcgc tctcgttgtg tagttaggga caaaaggacc 1740 ggtgggtcag atcggtgtga caagcaacgt ttggtcccga ctcgcaatcg cacctcggtg 1800 gcggtcacat ccgcgtcggt gtcagtcggt ccatccaggc ttgggagttg tctccggtct 1860 gccaaacggg tgggacggcc acggcgtcca acccattgcg gtgggcccgg gccatgtgac 1920 gacggtagga ccgcggtgag tgagaccgcc aatacccatg cagttgcccg ggcccctgat 1980 ctcggtatcg gctgacatcc caacagcggc cgtgtgcttg actgcgacca tgacatcgcc 2040 gatatcggcg gcaccactcg acgcccgcaa aacccgatgg cggggtgtag ttggggcggc 2100 gtgtttcctt cccgtctacc accacgcacc atggcgcttt gtccgacctg agggattcga 2160 ccacgcgctg atcagcatcg gcgtcgacat tcgctcgacc cccgccctcg agtacctcga 2220 ccagctaccg ctgcagttcg ttcggatcgt tgcacccgac gagggctcgg aagggtactg 2280 cgatctcgac ttcgcatacg cagcggtgct ggccagccat caccgccagg ctgcccgggt 2340 gttccccgac gggcggatcg ccggctccgt cgccgcccct gctcctgatt gccagcctgc 2400 gcgccgagct gctcgtcggg ctgagattct cctcggcggc accgagaccc aatcggtacc 2460 gacatctcat tcgcgccagg ctccggccgg tgcgcttact atccgatcat gattcctctc 2520 gacgtacgcc agttggagtg tttcatcgct gtcgccgaag aacgccacgt cggccatgcc 2580 gcagcccggc tgcacatgac ccagccgcct ttgacccgcc gaatctcgcg cctcgaacgc 2640 gaaatcggcg cacagctgtt cgtgcgcact ctgaccgggg tcgaactgac cgccgccggg 2700 ctcgtactgc tcgaccgcgc ctaccgaatt gtggcgctgg ccgagaacac cctcgaacgc 2760 gtgcgcctgg ccgacgccgg tgaagcgggc gcgctggccg tcggctactt cggctcgacg 2820 atcttcggac tcgtcccccg cctactgcgt ctcttcctcg acactcgccc cgaagtgcgg 2880 atcgtcatcg aacgcgcgac cagggacatg caggcccagg ccattcgcga tggccgcatg 2940 cacattggat tcagtcgcca ataccccgcc gaacctgacc tggcggtgcg caacatcgcc 3000 tccgagccgc tgtatgtggc actgcccgaa acacatccac tactgagcaa gcagaaggtg 3060 ctgctcgccg acatccagaa cgaaccgctg gtgctgttcc cttcggcacc caggccaggt 3120 ttcgccgacg aagtcgttca attactcatg cgcaccggcg gtgaaccgcg catcgaagcc 3180 gaagcaagcg acgtcgtcac cgccttagcc tacgtggcca tcgcacaatt gtgcgcggtg 3240 gtcccccgtt ccgccagcaa cattgcactg cccggcgtgg tgtacgtccc tctggccgac 3300 gccccgccgg aatcgctgag ctgcatctac cgggcaatcg agacaccgac gttggtgcgt 3360 gccttcctcg accatcttga cctcctggat ccggcgacgc tcgacctata cgaggactga 3420 ccgataccca caccgacgtg acgacggtgc ggtgttggta ttggcccacc cgagctgcgg 3480 ctggctacgc ttcacccagg cgcatcaaag cccctccgtc ccccgcagcc ccttccgacg 3540 cgaggcccgt cgcgacagca acggattcgc ctttccgcgc gtgcagtgcc tctttgtcgc 3600 cggcaaagag gcactgcgta agagatatcc aaggagttaa acatggccaa tacacgcgtg 3660 atcgagttgt tcgacgaatt caccgatctc atccgtgatt tcatcgtccg tcacgagatc 3720 accacaccgg agtacgaaac gatcatgcaa tacatgatca gcgtcggcga ggccggcgag 3780 tggccgctgt ggctcgacgc gttcttcgaa accaccgtcg actccgtgag ctacggcaag 3840 ggaaactgga cctccagtgc gatccaagga ccgttcttca aagaaggtgc cccgctgctg 3900 accggcaaac cggccaccct gccgatgcgg gcagacgagc ctggcgaccg gatgcggttc 3960 accgggagcg tgcgcgacac gtcgggaact ccgatcaccg gcgcggtcat cgatgtgtgg 4020 cactccacca acgacggcaa ctacagcttt ttctcccccg ctctgcccga ccagtacctg 4080 ctgcgcggca gggtcgtccc cgccgaagac ggcagcatcg agttccacag catccgaccg 4140 gtgccctacg aaatcccgaa agctggcccc acgggccagc tcatgaactc atacctcggc 4200 cggcactcct ggcgccccgc acacatccac atccgcatca ccgccgacgg gtaccggcca 4260 ctgatcaccc agctctactt cgagggcgac ccgtacctgg acagcgactc ctgctcggca 4320 gtcaagagcg aactggttct gccggtcaac aagatcgaca tcgacggcga aacatggcag 4380 ctagtcgact tcaacttcat tctccagcac aactgatcgc gagacatcat gtgccacaac 4440 aaatcatccg cgcctcccac acccgcacac atatcgatac agcaaaaccg gactcccggc 4500 accgaccccg ttccctacct gcacgcgtcc tcggacaccg ggagcggtga cacgatcgtc 4560 ctcctcaccg acatcttcgg tgtgaccccg ttctatcgac acctggctgc catgctcgcc 4620 gagaaaggcc acgacgtcgt cattcccgac gtattccacc gagtaggcca cgccaccgac 4680 ccgggccgcg acgcggcctt ggccagacgc cgccaattgg acgaccgtct cgcgatcgag 4740 gacatcgaac gcaccgtcgc ccacaccgtc gatgatcaac agacattcgg tgtactcgga 4800 ttctgcctcg gcggaagctt cgccctgctg accgcggccg cacaccccaa tcaggtcacc 4860 gccacctact acgccttccc taaaggcgcg ccaggagcga aagtgcctgt caaaccgcca 4920 cttgaggctg ccgatgcgat cgacgggccc gtcctgtgcc actgggggcg agacgactac 4980 atcgatcacg aggaaattga ccaactggaa cagatactcg cgtcggctcc cggtcccagc 5040 gagatccggt ggtacgacaa cgccgggcac agcttcctcg ccggactaac agaaccgaac 5100 caccccagca ctgcagccgc ccacgactcc tggcagcgca cagtggaatt cttcgaacgg 5160 tacctcaccg taggatccgc caactgaacg gaacggcgcc ctgccggcga ccacacaggc 5220 ccatttgccc ataccattcc tggtatacgt tgcagagcaa cggactgatc actgccttgt 5280 ggcgtgcccg tcaccgggcg gcgcagctgc tgtcacctct ctccggctta cccgctgagt 5340 ctgctcacag caccaacgcg caacctcgag cgtcggctcc tcgtgccatt cacgaccggc 5400 cccggtattc agaacccgcc tgcggaccag tccgcctgcg gttgggtggt tgttgtcgtc 5460 gggtggagaa ctcgcgagaa ctgggcccca ctcagcgccg gggtcgggaa acatcgttgt 5520 cggagtcacg cgttcggcac tcgccggtcg accaggcgca ctcagtaggt gtttggaaag 5580 ctgtgcgatt tcggccccga agcggatcgg tgacatcatg atggggcatg ggttctcgta 5640 agcagcatcg caagccgaag ccgtatccgt ccgatttgac cgatgcgcag tgggagttga 5700 tcgcaccgat ggtccctgta tcttcggctg gtcgcccgcc cattcactcg cgccgccgga 5760 tcgtggacgc gatcttgtat gtcaaccgca ccggctgttc ctggcgccag ttgccgcatg 5820 attttccgaa gtgggagacg gtgtactggt acttcaaggt gtggaacgag tctggggtga 5880 ccgaccggat ccacgacgca ctgcgggcgc gggcgcgcga tcgcggtggt cgggatccga 5940 tggcctcggc ggggatggtc gacgcccagg cggtgaaggg tgccgacacc gtcgggacgg 6000 atacgcgcgg ttgggacggc gcgaaacgag tcaatgggcg caaaaggcac ttggtgaccg 6060 acacgctcgg cctactcgtg gtggtgttgg tgacggcggg gagtgtgcag gaccgtgacg 6120 gcggacgccg ggtgctggcg cgggcgaaga cggcgatgcc gtcgatggtc gtggtgtggg 6180 ccgacggcgg ctacgccggg aagctggtcg cctgggtcga cacgctgccg catcgcagtg 6240 gatatcgttc ggaaacccaa agggcagagt gggtttgctg tcctaccgca ccgctgggtg 6300 atcgaacgga cgctgtcgtg gctggtgcgc tgtcgccgcc tcgaccacga ctatgagcgc 6360 ctgcccgcac actcggaggc gttcatcaag tgggccatga ttgggctcat gacacgacga 6420 ctcgcaccga caccgggacg ccgaccatgg caatcgaccc gcgcatcgtg accatttcca 6480 aacacttagt cagaggcatc tagcggcgct ccggcatgta ctcgggatac tcagccggtg 6540 gtgcaatcta gaagacagct tggtgaagcc ctggggccat ccgatatgga cctggtctac 6600 ctgtactgcc acggagagcg gtacaacccc ccgggagcat ccgtgtccga ccccctcctg 6660 gtcctcgggt ccggcgaatc gttcacaccc caggacgtca gcggatggtc ccagctctac 6720 gattggcccg atccgcactg gcgcgaccgc ccgcccctcg tcgtgctaaa cgcctgccac 6780 tccggagcga gcctggcgac aaccctcgct gatttcgtag gagtcttcac gcaaaccgcc 6840 ggcgccgctg gggtcatcgc caccgaggtc gcgcttgagc agaaagtggc cggactcgcc 6900 atggaaacct tcctgacgga gctcgtcatc ccccgcatct ccgtcggcga ggccatgcgc 6960 cgcatgcgct ggacgctcct caagcgtgga aatctcatgg gtctcgccta ctcgccgtac 7020 tgcgcagcag acctgctgct gtggcctgca acgactctgc aacacagagc cgacgaagcg 7080 ctaagcctgg atccgtggag ctacgccagg ccgtaacgac gtgtgactaa cgaaagcgcc 7140 tcccgtagtg agacaatcaa gattctcacg tcacaattgt ccacatacgg aagggcactt 7200 tcagtgcaat catcgcacac gttcacggct gggtccgcgg 7240 // |
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