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EBI DbfetchID AF002664; SV 1; linear; genomic DNA; STD; PRO; 2331 BP. XX AC AF002664; XX DT 21-JUN-1997 (Rel. 52, Created) DT 05-AUG-1998 (Rel. 56, Last updated, Version 2) XX DE Clostridium stercorarium alpha-L-arabinofuranosidase (arfB) gene, complete DE cds. XX KW . XX OS Clostridium stercorarium OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RX DOI; 10.1007/s002530050496 RX PUBMED; 7576552. RA Schwarz W.H., Bronnenmeier K., Krause B., Lottspeich F., Staudenbauer W.L.; RT "Debranching of arabinoxylan: properties of the thermoactive recombinant RT alpha-L-arabinofuranosidase from Clostridium stercorarium (ArfB)"; RL Appl. Microbiol. Biotechnol. 43(5):856-860(1995). XX RN [2] RP 1-2331 RX PUBMED; 9682483. RA Zverlov V.V., Liebl W., Bachleitner M., Schwarz W.H.; RT "Nucleotide sequence of arfB of Clostridium stercorarium, and prediction of RT catalytic residues of alpha-L-arabinofuranosidases based on local RT similarity with several families of glycosyl hydrolases"; RL FEMS Microbiol. Lett. 164(2):337-343(1998). XX RN [3] RP 1-2331 RA Schwarz W.; RT ; RL Submitted (05-MAY-1997) to the EMBL/GenBank/DDBJ databases. RL Technical University Muenchen, Institute for Microbiology, Arcisstr. 21, RL Muenchen D-80290, Germany XX DR StrainInfo; 110669; NCIB 11754. XX FH Key Location/Qualifiers FH FT source 1..2331 FT /organism="Clostridium stercorarium" FT /isolate="NCIB 11754" FT /mol_type="genomic DNA" FT /clone="pARF20" FT /db_xref="taxon:1510" FT RBS 326..333 FT /citation=[2] FT CDS 343..1818 FT /codon_start=1 FT /transl_table=11 FT /gene="arfB" FT /product="alpha-L-arabinofuranosidase" FT /function="hydrolysis of arabinose residues from FT arabinoxylan sidechain" FT /EC_number="3.2.1.55" FT /note="ArfB" FT /db_xref="GOA:O08457" FT /db_xref="InterPro:IPR010720" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:O08457" FT /citation=[2] FT /protein_id="AAC28125.1" FT /translation="MAKYVINTLNRKSKINREIYGHFSEHLGRCIYGGFYVGENSPIPN FT TKGIRNDVVNALKKIRIPVLRWPGGCFADEYHWMDGIGPKEQRKKMINTHWGGVVEASF FT GTDEFMELCRQLECEPYINGNLGSGTVKEMQDWIEYLTFDGISPMSELRAKNGRKEPYR FT LKYFCIGNENWGCGGNMRPEFYADMYRRYQTYVRNYGNNKIYKIACGPNSDDYNWTETV FT VKIAGRFMDAISLHYYTVPGTWHSKGSSTKFTEEEWYITLFKTLKMDELITKHTAIIRR FT YDPDNRIGLVVDEWGTWYDVEPGTNPGFLYQQNTMRDALVAGINLNIFNKHSDKVVMAN FT LAQTINVLQAVILTEGEKMLLTPTYHVFDMYKEHQGAELVEKLLLKTEKKSAQTKYTVP FT NLHESASVNADGKLHITLCNLSLSESYRVETEIIGKKAENVTGRILTGKMNDHNTFDAP FT ETVKPQTFDKISVRQNGLVIEIPPVSVVSITVD" FT terminator 2262..2296 FT /citation=[2] XX SQ Sequence 2331 BP; 715 A; 499 C; 514 G; 603 T; 0 other; aagcttttct ttaatgacat aatgtattta agcccggaag aaactgccgg ttttgtttca 60 tttcatccgc gctttatatg tcttttcggt ttaatgtatt accgaacaac tgccgttttg 120 ttaaaacgcc cccaagaatc tgacggtatg ctcaaatatg ctcaacaagt tatattattg 180 acatttctca gtttctaatg ctatcataca catagttaaa tataaattat tttatatttt 240 tctgaaaata tttttctaat ttattcctga tttttattcc gtcctttgta tttttcccga 300 taaaccggga ttattataaa ttattcaagg aggcttagga ttatggccaa atacgtaatc 360 aacaccctga acaggaagag caaaattaac agggaaatct acggtcactt ctcggaacac 420 cttggcaggt gcatatacgg cggtttttat gttggtgaaa attcccccat tccgaacacc 480 aaaggcatca gaaatgacgt ggttaacgct ctgaaaaaga taagaattcc ggtacttcgc 540 tggccgggcg gttgttttgc cgatgaatac cactggatgg acggaattgg cccgaaagaa 600 cagaggaaaa aaatgatcaa tacccactgg ggcggagttg tcgaagcaag ttttgggacc 660 gacgagttca tggaactttg ccgccagctg gaatgcgagc cttatattaa cggcaatctg 720 ggaagcggta ctgtaaagga aatgcaggac tggattgagt atctgacatt tgacggcata 780 tccccaatgt ctgaattaag ggcaaaaaac ggaagaaaag aaccttacag gttgaaatat 840 ttctgcatag gcaacgaaaa ctggggctgc ggcggaaaca tgagacctga gttctatgcc 900 gacatgtaca gaagatacca gacttacgta agaaactacg ggaataacaa gatatacaaa 960 atcgcctgcg ggccgaacag cgatgattac aactggacag aaacggttgt taaaattgcg 1020 ggcagattca tggacgccat ctcgctgcat tattacacag tgccgggaac ctggcattcg 1080 aagggcagtt ccacgaaatt caccgaagaa gaatggtata taaccctgtt taaaactctc 1140 aaaatggatg agctgatcac taaacatacc gcgataataa gaagatacga ccccgacaac 1200 cgcatagggc tggttgtgga tgaatggggt acatggtatg acgttgagcc cggaacaaat 1260 cccggattcc tgtaccagca aaatacaatg agagacgcgc ttgttgcggg gattaacctt 1320 aacatcttta acaaacacag cgacaaagtt gtaatggcaa acctcgctca aaccattaac 1380 gttcttcagg cagtgatcct taccgaaggc gaaaaaatgc tcctcacacc cacataccat 1440 gtgtttgaca tgtataagga gcaccagggc gcggaactgg ttgaaaagct tttattgaaa 1500 acggaaaaaa agtcggcaca gacaaaatat accgttccga acctgcatga atccgcttcg 1560 gtgaatgccg acggaaaact gcacattact ctgtgcaacc tgtccctcag cgaaagctac 1620 agagtagaaa ccgaaatcat aggaaagaaa gcggaaaacg ttaccggacg cattttgaca 1680 ggaaagatga acgatcataa taccttcgac gcacctgaaa cagttaagcc ccagacattt 1740 gacaaaatat ccgttagaca gaacggcctt gttattgaaa taccacccgt cagcgtagtc 1800 agcattacgg ttgactgatt tattgcagga gcgacatatg gaacaaccgt gcagaacatg 1860 cctttttaac agtattgaaa gagatcagat cgaaaaaaac ataaaggaat atattgaaag 1920 tctttcaccg gatgaaaaag ccggtgaaag catttataac aaccgtctta ggctgtgttt 1980 tgaatgcccg aactgttttg acgggctgtg cagaatatgc ggttgcttcg tccgggcaag 2040 agcggccaag ctgagatcat actgtcccga ccctgcaaaa aaggtggtag cccgtttacc 2100 tgataaaatc ggttcttatg ggcttttctc cttttgggaa gactattccc ttttcccttc 2160 ccgcttctat gcatttcaga agccatgcca tgttattgcc aagtgtccgc attgtctgca 2220 tgccttcctc atctttcata acttcttcag gggtgctgcc gtgtacctga ttccagtact 2280 gggatgaaac caccggcatt ccgctgattg tgaaatattt gtttatctga t 2331 // |
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