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EBI DbfetchID AF001268; SV 1; linear; genomic DNA; STD; PRO; 2627 BP. XX AC AF001268; XX DT 18-JUN-1997 (Rel. 52, Created) DT 03-MAR-2000 (Rel. 62, Last updated, Version 4) XX DE Pyrococcus furiosus alpha amylase (amyA) gene, complete cds. XX KW . XX OS Pyrococcus furiosus OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-2627 RX PUBMED; 9293008. RA Dong G., Vieille C., Savchenko A., Zeikus J.G.; RT "Cloning, sequencing, and expression of the gene encoding extracellular RT alpha-amylase from Pyrococcus furiosus and biochemical characterization of RT the recombinant enzyme"; RL Appl. Environ. Microbiol. 63(9):3569-3576(1997). XX RN [2] RP 1-2627 RA Dong G., Vieille C., Savchenko A., Zeikus J.G.; RT ; RL Submitted (24-APR-1997) to the EMBL/GenBank/DDBJ databases. RL Biochemistry, Michigan State University, 410 Biochemistry Building, East RL Lansing, MI 48824, USA XX DR StrainInfo; 121216; DSM 3638. XX FH Key Location/Qualifiers FH FT source 1..2627 FT /organism="Pyrococcus furiosus" FT /strain="DSM 3638" FT /mol_type="genomic DNA" FT /db_xref="taxon:2261" FT CDS 676..2058 FT /codon_start=1 FT /transl_table=11 FT /gene="amyA" FT /product="alpha amylase" FT /EC_number="3.2.1.1" FT /note="alpha-1,4-glucan 4-glucanohydrolase; acidic FT extracellular enzyme" FT /db_xref="GOA:O08452" FT /db_xref="InterPro:IPR006046" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR013776" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR015237" FT /db_xref="InterPro:IPR017853" FT /db_xref="PDB:1MWO" FT /db_xref="PDB:1MXD" FT /db_xref="PDB:1MXG" FT /db_xref="UniProtKB/TrEMBL:O08452" FT /protein_id="AAC45663.1" FT /translation="MNIKKLTPLLTLLLFFIVLASPVSAAKYLELEEGGVIMQAFYWDV FT PGGGIWWDHIRSKIPEWYEAGISAIWLPPPSKGMSGGYSMGYDPYDYFDLGEYYQKGTV FT ETRFGSKEELVRLIQTAHAYGIKVIADVVINHRAGGDLEWNPFVGDYTWTDFSKVASGK FT YTANYLDFHPNELHCCDEGTFGGFPDICHHKEWDQYWLWKSNESYAAYLRSIGFDGWRF FT DYVKGYGAWVVRDWLNWWGGWAVGEYWDTNVDALLSWAYESGAKVFDFPLYYKMDEAFD FT NNNIPALVYALQNGQTVVSRDPFKAVTFVANHDTDIIWNKYPAYAFILTYEGQPVIFYR FT DFEEWLNKDKLINLIWIHDHLAGGSTTIVYYDNDELIFVRNGDSRRPGLITYINLSPNW FT VGRWVYVPKFAGACIHEYTGNLGGWVDKRVDSSGWVYLEAPPHDPANGYYGYSVWSYCG FT VG" XX SQ Sequence 2627 BP; 728 A; 511 C; 600 G; 788 T; 0 other; ttcttggtaa aacttcctct tcaagcatcg ccttcatttc tcttggcatg cctgggagca 60 caaagatttt agtgcctcta tattctatat aggccccagg tgctgcccca acagagtttt 120 tcaatggaat ggctcctttg ggaaggtaag ccatcttttt ccttgcatca ttcagcgtag 180 gatcatcaat tagcccctcc ctatataatc tctcataaaa ttcctttatt tcctctaagc 240 atttttcaca gagcactaat tcttttccca gcgccttagc gacggccacc attgtaacgt 300 catcgtgagt tggcccgagg cctcccgaaa taataagaac atcgggtttt ctgttaaggg 360 attctaaaat tacagacctt atatcctcca cgtcatcgcc aacagtagtt attctcttaa 420 cgaggtatcc tctctccgtt aatttcttcg ctatgtgtgc tgagttgctg tttacagtat 480 tccctgttaa aagttcatca cctacggtaa ttatttccgc gaacattggt tctccccagg 540 aattgttttt atcaagagtt tattagattt tgacgtgcgt tgatgaacat ttatgttcac 600 atgatcataa cagaaaattt atatgtatca tcaccagtga tacattatga gactttggtg 660 tatggaggtg atcacgtgaa cataaagaaa ttaacacccc tcctaactct attactgttt 720 tttatagtac tagcaagtcc agtaagtgca gcaaaatact tggagcttga agagggagga 780 gttataatgc aagcattcta ttgggatgtt ccagggggag gaatttggtg ggatcatata 840 agatcgaaga ttcctgaatg gtatgaagct ggaatctctg caatatggct acctccacca 900 agcaagggga tgagtggagg atattcaatg ggctacgatc cctatgatta ctttgatctc 960 ggcgagtact accagaaggg aactgtagag acgcgttttg gatcaaaaga agaactagtg 1020 agattgatac aaactgccca tgcctatgga ataaaggtaa tcgccgatgt agttataaac 1080 cacagggctg gtggtgacct agaatggaac cccttcgttg gagattacac atggacagac 1140 ttttctaaag ttgcctcagg gaaatataca gctaactatc tggacttcca tccaaacgag 1200 cttcattgtt gtgacgaagg aacctttgga ggatttccag atatatgtca tcacaaagag 1260 tgggatcagt actggctatg gaagagcaat gagagttatg ctgcttattt aagaagcata 1320 ggatttgatg gttggagatt tgactatgtt aagggctatg gagcttgggt tgtcagagac 1380 tggcttaatt ggtggggagg ttgggcagtt ggagagtact gggacacaaa tgtagatgca 1440 ctactaagct gggcatatga gagtggtgca aaggtctttg acttcccgct ctactataaa 1500 atggatgaag catttgacaa taacaacatt ccagcattag tctatgccct acaaaacgga 1560 caaactgtag tttcgagaga tccatttaag gcagtaactt tcgttgccaa tcatgacaca 1620 gatataatat ggaacaagta tccagcatat gcgttcatat tgacatatga gggacagcca 1680 gtaatattct acagggactt tgaggaatgg ctgaacaagg ataagctaat taacctcatt 1740 tggatccatg atcatttggc aggaggaagc acaacaattg tctactacga caacgatgag 1800 ctcatatttg tgagaaatgg agattctaga aggcctgggc ttataactta cattaacttg 1860 agccctaact gggttggtag gtgggtatac gttccaaagt ttgcaggggc ttgtattcat 1920 gaatacactg gaaacctagg aggatgggta gataaaagag tagatagtag cggatgggta 1980 tacctagagg caccacctca cgatccagct aacggctact atgggtactc cgtatggagt 2040 tattgtggtg ttgggtgact ttttcttttt tctttttaac aatgggagaa gtgcaaatac 2100 tgcgacaatt cctgggccgc atacaggagt ttctggagcg gcttcattca atattactgt 2160 tttatttcca attccataca acgtgacatt tgttggttgg agaggttccc agggaagtcc 2220 cttatatagt tgaactgtaa aagttttgtt tctggggagg ggaaccacgg caaagtagtg 2280 tcccgttgga gatttcaaga gggggtctcg gctctgagag ttctggtatg ttccccttga 2340 cccaaaggta accagtgtag tttccttgta ggactattag tgctgggata tcattgctag 2400 aatagtacct aagcttaacc tcattgcttc tagttccatt aacaacaacc caaacaggaa 2460 tccacgcatt catagtttta atctcgggaa cctctacaag gatggaattt tcttctttct 2520 caataattct tgctctccta cctcctatat acaccttgat gtttccattg aggcctgctc 2580 cggctataag gattttttgc ccaggttggg ctatgtatgg agttata 2627 // |
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