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EBI DbfetchID AB304464; SV 1; linear; genomic DNA; STD; PRO; 4962 BP. XX AC AB304464; XX DT 10-AUG-2007 (Rel. 92, Created) DT 29-OCT-2007 (Rel. 93, Last updated, Version 2) XX DE Bacillus subtilis subsp. natto iolR, IS4Bsu1, iolA, iolB genes for DE hypothetical proteins, transposase, complete cds. XX KW . XX OS Bacillus subtilis subsp. natto OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4962 RA Kimura K., Itoh Y.; RT ; RL Submitted (21-MAY-2007) to the EMBL/GenBank/DDBJ databases. RL Keitarou Kimura, National Food Research Institute, Applied Microbiology RL Division; Kannondai 2-1-12, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:keitarou@affrc.go.jp, Tel:81-29-838-8076, Fax:81-29-838-7996) XX RN [2] RX DOI; 10.1271/bbb.70223 RX PUBMED; 17928718. RA Kimura K., Itoh Y.; RT "Determination and characterization of IS4Bsu1-insertion loci and RT identification of a new insertion sequence element of the IS256 family in a RT natto starter"; RL Biosci. Biotechnol. Biochem. 71(10):2458-2464(2007). XX FH Key Location/Qualifiers FH FT source 1..4962 FT /organism="Bacillus subtilis subsp. natto" FT /sub_species="natto" FT /strain="Miyagino (=Miura)" FT /mol_type="genomic DNA" FT /db_xref="taxon:86029" FT CDS complement(<1..544) FT /codon_start=1 FT /transl_table=11 FT /gene="iolR" FT /product="hypothetical protein" FT /db_xref="GOA:A7BJC2" FT /db_xref="InterPro:IPR018356" FT /db_xref="UniProtKB/TrEMBL:A7BJC2" FT /protein_id="BAF74774.1" FT /translation="MKLMRIQEMEEYILSHGTVSLDELCQVFNVSKNTVRRDINKLTEK FT GAIEKVYGGVTSIEKTALVPFENRTIQHQDEKTKIAHYASRFIEDHDLVFIDSGTTTKS FT ILDTLDPAKNVTILTNSLDIINAASALKNINLIIIGNNYKRKTRSFVGMDDPAMLDKYN FT INKAFMSATGTTLTDLQA" FT CDS 838..1962 FT /codon_start=1 FT /transl_table=11 FT /gene="IS4Bsu1" FT /product="transposase" FT /db_xref="GOA:A7BJB2" FT /db_xref="InterPro:IPR014736" FT /db_xref="UniProtKB/TrEMBL:A7BJB2" FT /protein_id="BAF74775.1" FT /translation="MDKFTRKTSFEQWFSPIFSTKLEELVESYQLNYYTKKLHIASFLK FT LFVFAQLNETESLRAVSETLFSDELQKATHLEAISFSQLGRRLNQVPTEVFQQVFLDLV FT AQIHEKSHYEQRRETTTPLKIIDSSTLPLNLKNHKWAEFRKTKSGIKLHLRLVYAEKGC FT SYPDKAVLTNAKEHDRGQLEVLVDDKECMYVFDRGYLDYERFDRMTDDGYFFVSRLRKN FT AVIRVIEPFKLPEDSLVFSDEMVLIGTPQNRAENAFRLIKVLDSKGNELHLITNRFDLS FT ADEIAELYKSRWAIELFFKWLKQHLNIKKFYAQSEQGVHNQVYIAMIVYCLNVLAQLNT FT NSSRTYLQISRLLKAAPWKSAHLWLRKIVGKTVP" FT CDS 2346..3839 FT /codon_start=1 FT /transl_table=11 FT /gene="iolA" FT /product="hypothetical protein" FT /db_xref="GOA:A7BJC4" FT /db_xref="InterPro:IPR010061" FT /db_xref="UniProtKB/Swiss-Prot:A7BJC4" FT /protein_id="BAF74776.1" FT /translation="MCLLMKEAIRMAEIRKLKNYINGEWVESKTDQYEDVVNPATKEVL FT CQVPISTKEDIDYAAQTAAEAFETWSKVAVPRRARILFNFQQLLSQHKEELAHLITIEN FT GKNTKEALGEVGRGIENVEFAAGAPSLMMGDSLASIATDVEAANYRYPIGVVGGIAPFN FT FPMMVPCWMFPMAIALGNTFILKPSERTPLLTEKLVELFEKAGLPKGVFNVVYGAHDVV FT NGILEHPEIKAISFVGSKPVGEYVYKKGSEHLKRVQSLTGAKNHTIVLNDANLEDTVTN FT IVGAAFGSAGERCMACAVVTVEEGIADEFMAKLQEKVADIKIGNGLDDGVFLGPVIRED FT NKKRTLSYIEKGLEEGARLVCDGRENVSDDGYFVGPTIFDNVTTEMTIWKDEIFAPVLS FT VIRVKNLKEAIEIANKSEFANGACLFTSNSNAIRYFRENIDAGMLGINLGVPAPMAFFP FT FSGWKSSFFGTLHANGKDSVDFYTRKKVVTARFPAPDFN" FT CDS 3914..4729 FT /codon_start=1 FT /transl_table=11 FT /gene="iolB" FT /product="hypothetical protein" FT /db_xref="GOA:A7BJC5" FT /db_xref="InterPro:IPR010669" FT /db_xref="UniProtKB/Swiss-Prot:A7BJC5" FT /protein_id="BAF74777.1" FT /translation="MSYLLRKPQSHEVSNGVKLVHEVTTSNSDLTYVEFKVLDLASGSS FT YTEELKKQEICIVAVTGKITVTDHESTFENIGTRESVFERKPTDSVYISNDRAFEITAV FT SDARVALCYSPSEKQLPTKLIKAEDNGIEHRGQFSNKRTVHNILPDSDPSANSLLVVEV FT YTDSGNWSSYPPHKHDQDNLPEESFLEETYYHELDPGQGFVFQRVYTDDRSIDETMTVE FT NENVVIVPAGYHPVGVPDGYTSYYLNVMAGPTRKWKFYNDPAHEWILER" XX SQ Sequence 4962 BP; 1518 A; 1013 C; 1116 G; 1315 T; 0 other; atgcctgcag gtctgtcagc gtcgttccgg tagcggacat aaaggctttg ttgatattgt 60 acttatcaag catagccggg tcgtccatgc cgacaaagga gcgggttttt ctcttatagt 120 tgtttccgat aatgattaag ttgatattct tcaatgctga cgccgcattg ataatgtcta 180 aactgttggt taagatggtg acatttttgg ctggatcaag cgtatcaaga atagactttg 240 tcgtcgttcc tgaatcaatg aagaccaaat cgtgatcttc aataaatcgg gaagcataat 300 gggcgatttt tgttttttca tcctggtgct gaatggtgcg attttcaaaa ggaactaatg 360 cggttttttc aatagatgtt acgccgccgt atactttttc aatcgcaccc ttttctgtta 420 gcttgttgat atcacgtctg actgtgtttt tggagacatt gaacacctgg cacagctcat 480 ctaaggaaac agtgccatgc gataaaatgt actcctccat ttcctgaatc cgcatcagtt 540 tcatattaaa aactccttct tgaatcttta cgtaacataa atatataata aaaaccaaaa 600 gttaatcaat agttgcttaa aaaatgataa catcggtgta tatcttttgc aagattggac 660 ggccttctct tacctctctt acttgattaa aagattaata tcactagtgt cgcattaaaa 720 taattcaacg atttataaaa atctaaattt tcagtaattt tatctttgca taaaaaaagc 780 ctttataatg gtagggcggt aataaaccat ccaaatccaa aataaaggac aataagcatg 840 gataagttta cacgaaaaac atcatttgaa caatggtttt caccgatttt ctccacaaaa 900 ctcgaggaat tggttgaaag ctatcaatta aattactata caaagaagct acatattgcg 960 tcattcttga aattgttcgt attcgctcag ctcaatgaaa ccgagagtct gcgtgcagtc 1020 agtgagacat tattttctga cgagcttcaa aaagcgacgc atttagaagc gattagcttt 1080 tcgcagctcg gacgccgatt aaatcaagtg ccgactgaag tgttccaaca ggtatttttg 1140 gatttagttg cccaaattca cgagaaatca cattatgagc agcgccgaga aacgacaaca 1200 ccactgaaaa ttatcgactc gagtacgttg ccattgaact tgaaaaacca taagtgggct 1260 gaattccgta aaacaaagtc gggcatcaag cttcatttac gtcttgttta cgcagaaaaa 1320 ggctgttcct atccagataa agcggtgctg acaaacgcga aagaacatga tcgtggtcag 1380 ctggaagtac tcgttgacga caaagaatgc atgtacgtct ttgaccgggg ttacttggat 1440 tatgaacgat ttgaccgcat gacagacgat gggtatttct ttgtgtcacg cttacgaaaa 1500 aacgctgtca ttcgtgtaat cgagccattt aaactgcctg aagactcgct tgtgttttca 1560 gatgaaatgg ttttgattgg tacaccacaa aaccgtgcag aaaacgcttt tcgcctcatc 1620 aaagtgctcg attcaaaagg aaacgaactg catctgatca caaatcgatt tgatttaagc 1680 gctgacgaaa tcgctgagtt atataagtcg cgctgggcaa tcgagctatt ttttaaatgg 1740 ctgaagcagc acctgaacat caaaaagttc tacgcgcaga gcgaacaagg cgtacataat 1800 caagtgtata tcgcaatgat tgtatattgc ctaaatgtac tggcacagct gaacacgaac 1860 agttcaagga cgtacttaca aattagtcgc ttactcaaag cggcaccatg gaaatccgct 1920 catttatggc tacgaaaaat cgtagggaaa accgtgccct aaagaatttc acaggtctgt 1980 tgcactcgtc aaagattaga gtaaaaaaat ggatgttaac tacctctatt caaatgtctt 2040 tgtttttctt ctcatgagta aaaacaagaa agcagaaaat tcagtcaaaa tttatgcaac 2100 actagtggat taatataata aaaataatga aaaaatgtag tgtttatgat tgacttatgg 2160 gtataatgcg attagaatat aaccaagaaa tgaccaaaat ttaacctgtt gtgcatgaaa 2220 tggttgcttg tatgtaaata tgcatccgcc atttattttt ttggtttttt tgaaagcgtt 2280 taattcttgg cttactgaaa agtggatatt gatatgaatc tatcctgaac catccataca 2340 agtgtatgtg tttactaatg aaggaggcaa taagaatggc agaaatcaga aaattaaaaa 2400 actacatcaa cggtgaatgg gttgaaagca aaacagatca atatgaagat gtcgtcaatc 2460 cggcgacgaa agaagtacta tgccaagtgc cgatttctac aaaagaggat attgattacg 2520 cagcgcaaac ggcggctgaa gcgtttgaaa catggtcaaa ggtggcggtt ccgcgccgtg 2580 ccagaattct atttaacttt cagcagctgc tgtcccagca taaagaagaa ctggctcatc 2640 tgattaccat tgaaaacgga aaaaacacaa aagaagcttt aggtgaagtg ggacgcggga 2700 ttgaaaacgt agagttcgcc gctggagcac cttccctgat gatgggtgac tcacttgctt 2760 ccatcgcaac ggacgttgaa gcggcgaact accgttaccc aatcggagtt gtcggcggaa 2820 tcgcgccatt caacttcccg atgatggttc cttgctggat gttcccgatg gcgatcgcgc 2880 tcggcaacac atttatttta aagccatctg agcggactcc gctgttaaca gagaaattgg 2940 ttgagctttt tgaaaaagcg ggccttccga aaggggtatt caacgtcgta tacggtgcgc 3000 atgacgttgt gaacggcatc ctcgagcatc ccgaaattaa agcgatttca tttgtaggct 3060 ccaagccagt cggcgaatac gtctacaaaa aaggaagcga acacttaaaa cgcgttcaat 3120 ctctgactgg tgcgaaaaac catacgattg tcttgaacga tgcgaatctt gaagacacgg 3180 tcacaaacat cgtcggagct gccttcggtt ctgccggtga acgctgcatg gcttgtgcgg 3240 ttgtgacagt tgaagaagga atcgccgatg aatttatggc gaagctgcag gaaaaagtgg 3300 cggacattaa aatcggtaac ggccttgatg acggcgtgtt cttaggaccg gtgattcgcg 3360 aagacaacaa gaagcgcacg ctcagctata tcgaaaaagg ccttgaagaa ggtgccagac 3420 tcgtatgtga cggacgtgaa aatgtgtctg acgacggcta ctttgtcggc ccgacgatct 3480 ttgacaatgt cacaacagag atgacgatct ggaaagatga aattttcgct ccggtcttat 3540 ctgtcatccg tgtgaaaaac ctgaaagaag cgattgaaat cgccaataag tctgagtttg 3600 cgaacggtgc ctgcttgttc acatccaatt caaacgcaat ccgctacttc cgtgaaaaca 3660 ttgatgcggg aatgctaggc atcaaccttg gtgtgccggc tccaatggcg ttcttcccat 3720 tctcaggttg gaaatcttca ttctttggaa cgctgcatgc gaacggaaaa gacagcgtcg 3780 acttctatac acgtaaaaaa gtggtaacgg caagatttcc ggcacctgat ttcaactaaa 3840 cgacgaacag ccgaacggcg tccatgtgcg ctgttcggac tcattctgcc aatagaaaga 3900 ggagggcttc tctatgagtt atttgttgcg taagccgcag tcgcatgaag tgtctaatgg 3960 ggtcaaactc gtgcacgaag taacgacatc caactctgat ctcacttatg tagagtttaa 4020 agtgttagat cttgcatcag gttcaagcta tacagaagaa ttgaaaaaac aagaaatctg 4080 tattgtggcg gtaacgggaa aaattacagt gacagatcat gagtcgactt ttgagaatat 4140 cggcacgcgt gaaagcgtat ttgaacgaaa accgacagac agcgtctata tatcaaatga 4200 ccgtgcattt gagatcacag cagtcagcga cgcaagagtg gcgctttgct attctccatc 4260 ggaaaagcag cttccgacaa agctgatcaa agcggaagac aacggaattg agcatcgcgg 4320 gcaattttca aacaaacgta ctgttcataa cattcttccg gattcagacc cttcagctaa 4380 cagtctatta gtagttgaag tctatacaga cagcggcaac tggtccagct acccgcctca 4440 caaacatgat caagacaact tgccggaaga atctttctta gaagaaacgt actaccatga 4500 gttagacccg ggacagggct ttgtatttca gcgcgtatac acagatgacc gttctattga 4560 cgagacaatg actgtggaaa atgaaaacgt tgtcatcgtt cctgcgggat accacccggt 4620 aggcgttccg gacggataca catcctacta tttaaatgtc atggcagggc cgacgcgaaa 4680 atggaagttt tataacgacc cggcgcatga gtggatttta gaacgctaac aagtgaggag 4740 tggctgttta cgatgaagta tacattcaat gaagagaagg cttttgacat tattgccatc 4800 ggccgggcat gtattgacct gaacgcagtc gaatacaacc gcccaatgga agagacgatg 4860 acattttcca aatacgtcgg cggttcacct gcgaatatcg cgatcggcag cgcgaagctt 4920 ggcttaaaag cgggcttcat cggcaaaatt ccggatgacc ag 4962 // |
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