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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB220485; SV 1; linear; mRNA; STD; MAM; 2501 BP.
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AC   AB220485;
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DT   07-JAN-2006 (Rel. 86, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 2)
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DE   Macaca fascicularis mRNA, clone QmoA-14231: similar to Homo sapiens caspase
DE   3, apoptosis-related cysteine protease (CASP3), transcript variant alpha,
DE   mRNA, NM_004346.2.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-2501
RA   Hashimoto K., Osada N., Kusuda J.;
RT   ;
RL   Submitted (04-JUL-2005) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Biomedical Innovation, Division
RL   of Biomedical Resources; 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan
RL   (E-mail:khashi@nih.go.jp, Tel:81-72-641-9014, Fax:81-72-641-9016)
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RN   [2]
RA   Kobayashi M., Tanuma R., Hirata M., Osada N., Kusuda J., Sugano S.,
RA   Hashimoto K.;
RT   "Analysis of gene expression in cynomolgus monkey tissues by macaque cDNA
RT   oligo-chips";
RL   Unpublished.
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CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC          QccE: cerebellum cortex
CC          QnpA: parietal lobe
CC          QtrA: temporal lobe right
CC          QflA: frontal lobe left
CC          QmoA: medulla oblongata
CC          QbsA(B): brain stem
CC          QorA: occipital lobe right
CC          QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing.
CC   The full-insert sequencing was done by primer-walking method using
CC   ABI DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..2501
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque brain cDNA library QmoA"
FT                   /clone="QmoA-14231"
FT                   /tissue_type="medulla oblongata"
FT                   /db_xref="taxon:9541"
FT   CDS             82..915
FT                   /codon_start=1
FT                   /transl_table=1
FT                   /product="hypothetical protein"
FT                   /note="similar to Homo sapiens caspase 3, apoptosis-related
FT                   cysteine protease (CASP3), transcript variant alpha, mRNA,
FT                   NM_004346.2."
FT                   /db_xref="GOA:Q2PFV2"
FT                   /db_xref="InterPro:IPR015917"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q2PFV2"
FT                   /protein_id="BAE73018.1"
FT                   /translation="MENTENSVDSKSIKSLEPKIIHGSKSVDSGISLDNSYKMDYPEMG
FT                   LCIIINNKNFHKSTGMASRSGTDVDAANLRETFINLKYGVRNKNDLTREEIVELMRNVS
FT                   KEDHSKRSSFVCVLLSHGEEGIIFGTNGPLDLKKITSFFRGDCCRSLTGKPKLFIIQAC
FT                   RGTELDCGIEADSGVEDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAM
FT                   LKQYADKLEFMHILTRVNRKVATEFESFSLDATFHAKKQIPCIVSMLTKELYFYH"
FT   polyA_site      2484..2501
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SQ   Sequence 2501 BP; 795 A; 426 C; 543 G; 737 T; 0 other;
     agacgcggat cgtagctggt ggctgctgct gtgaggcggt tgtggaaggt gtgaggaggc        60
     tgtgcgttaa taaaggtatc catggagaac actgaaaact cagtggattc aaaatccatt       120
     aaaagtttgg aaccaaaaat catacatgga agcaaatcag tggactctgg aatatccctg       180
     gacaacagtt ataaaatgga ttatcctgag atgggtttat gtataataat taataataag       240
     aattttcata aaagcactgg aatggcatct cggtctggta cagatgttga tgcagcaaac       300
     ctcagggaaa cattcataaa cttgaaatac ggagtcagga ataaaaacga tcttacacgt       360
     gaagaaattg tggaattgat gcgtaatgtt tctaaagaag atcacagcaa aaggagcagt       420
     tttgtttgtg tgcttctgag ccatggtgaa gaaggaataa tttttggaac aaatggacct       480
     cttgacctga aaaaaattac aagctttttc agaggggatt gttgtagaag tctaactgga       540
     aaacccaaac ttttcattat tcaggcctgc cgtggcacag aactggactg tggcattgag       600
     gcagacagtg gtgttgagga tgacatggcg tgtcataaaa tcccagtgga ggccgacttc       660
     ttgtatgcat actccacagc acctggttat tattcttggc gaaattcaaa ggatggctcc       720
     tggttcatcc agtcgctttg tgccatgctg aaacagtacg ccgacaagct tgaatttatg       780
     cacattctta cccgggttaa ccgaaaggtg gcaacagaat ttgagtcctt ttcccttgat       840
     gctacttttc atgcaaagaa acagattcca tgtattgttt ccatgctcac aaaagaactg       900
     tatttttatc actaaagaaa tggttggttg gtggtttttt tcagtttgta tgccaagtga       960
     gaagacggta tatttggtac ttatttccct ctcattttga cctagtctca tactgcagag      1020
     ggtactttaa gtcatactcc ttccatcagg tggaaccact gtgaagctac ctcaaactta      1080
     gagccaggta gttgcaattg aattaggaat aaataaaaat ggatactggt gcagtcatta      1140
     tgagaggcag tgattgttaa tttacagctt tcatgattag caagttgcag tgatgctgtg      1200
     ctatgaattt tcaagtaatc gtgaaaaagt taaacattga agtaatgagt ttttatgata      1260
     ttccccccac ttaagactgt gtattctagt tttgtcaaac tatagaaatg atgatgtgga      1320
     agaacttagg cgtctatggg catggtcaaa ggctcaaacc ttttgtttag tattgatata      1380
     caaaggtgac ctaactgcat ttttagacca tttatttggg attatggttt tgcgatgtct      1440
     gtcctgaaca cttttgttgt aaaaaataat aataataata ataatgttta atattaagaa      1500
     agaaactaag tattttatgt gagaggaagt gtgaacaaac ttgactcttt taagactaaa      1560
     acataacact cctagaggat ggagttttaa ctgtaaggtg ctacagtgca gctgatctac      1620
     cagcaaaaat accttctgat ttgtccctat gcagatcagg tgagcttcac ataccagcag      1680
     tctatctgaa gagctgtgat atgaaaagcc caaactgttg attattagcc aggtaatgtg      1740
     agtaaattat ataggaacat atgaaaatac aacttaaata ataaaaaatg gaatataagg      1800
     aaagcaataa atggatgggc tgagctgcct ctaacttgcg agcagatggt ttgatcttga      1860
     gcagagacag gactcggcct gttccgtgaa ggcagagcca tggagcatgg tttcaggaag      1920
     ggcctgcagc ccgcttctcc atacgcactg gtgtgtgtgg acgatgccgt cagggcacca      1980
     ttgccaagta agaaagtgaa gcaaatcaga aacttgtgaa gtggaaatgt tctaaatgtg      2040
     gtgagttaca ataaaaaatc atagtactct ttgtagcaca attcttaagc atgttatttt      2100
     ctgttgaagt ttacaatcaa aggaaaatag tatattttat actgtttact gaaagaaaaa      2160
     gaccacatag gactctagat ggtatccagc cgcaggccag agctgagcac ccagcctggg      2220
     aggcaagctc aggcctcaca tgtgctgagc cgtcactcac caagtctcac tggcttccag      2280
     tctgacattt cacaggagat ttcttgttgc tcaaaaaatg aactcgcatt tgtcaatgac      2340
     tgtttttctt actagatctg taacttttct aaatacacat agcatgtaat ggtatctcat      2400
     taaagtgtgt tcctgtgtga caattttgta caaatttgct attttccatt tttatttcaa      2460
     aatacacatt caaacttaaa attaaaaaaa aaaaaaaaaa a                          2501
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