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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB179294; SV 1; linear; mRNA; STD; MAM; 1237 BP.
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AC   AB179294;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
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DE   Macaca fascicularis testis cDNA clone: QtsA-16934, similar to human
DE   proteasome (prosome, macropain) subunit, alpha type, 1(PSMA1), transcript
DE   variant 2, mRNA, RefSeq: NM_002786.2.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-1237
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (10-MAY-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of:Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo,
CC   Tokyo, Japan; Division of Genetic Resources, National Institute
CC   of Infectious Diseases of Japan, Tokyo, Japan; National Health
CC   Research Institute, Taipei, Taiwan; Institute of Molecular Biology,
CC   Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution,
CC   University of Chicago, Chicago, IL, USA, Center for Information
CC   Biology,
CC   National Institute of Genetics of Japan, Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert
CC   sequencing was done by primer-walking method using ABI DNA
CC   sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1237
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QtsA"
FT                   /clone="QtsA-16934"
FT                   /db_xref="taxon:9541"
FT   CDS             82..873
FT                   /note="Homo sapiens proteasome (prosome, macropain)
FT                   subunit, alpha type, 1(PSMA1), transcript variant 2, mRNA,
FT                   RefSeq: NM_002786.2"
FT                   /db_xref="GOA:Q4R3H2"
FT                   /db_xref="InterPro:IPR001353"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q4R3H2"
FT                   /protein_id="BAE02345.1"
FT                   /translation="MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAV
FT                   LVALKRAQSELAAHRKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLP
FT                   VSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGA
FT                   RSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFT
FT                   IYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH"
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SQ   Sequence 1237 BP; 394 A; 229 C; 266 G; 348 T; 0 other;
     agatcgctga gcgatacttt cggcagcacc tccttgattc tcagttttgc tggaggccgc        60
     aaccgggccc gcgccgccaa catgtttcga aatcagtacg acaatgatgt cactgtttgg       120
     agcccgcagg gcaggattca tcagattgaa tatgcaatgg aagctgttaa acaaggttca       180
     gccacagttg gtctgaaatc aaaaactcat gcagtgttgg ttgcattgaa aagggcacaa       240
     tcagagcttg cagctcatcg gaaaaaaatt ctccatgttg acaaccatat tggtatctca       300
     attgcggggc ttactgctga tgctagactg ttatgtaatt ttatgcgtca ggagtgtttg       360
     gattccagat ttgtatttga tagaccactg cctgtgtctc gtcttgtatc tctaattgga       420
     agcaagaccc agataccaac acaacgatat ggccggagac catatggtgt tgggctgctt       480
     attgccggtt atgatgatat gggccctcac attttccaaa cctgtccatc tgctaactat       540
     tttgactgca gagccatgtc cattggagcc cgatcccagt cagctcgtac ttacttggag       600
     agacatatgt ctgaatttat ggagtgcaat ttaaatgaac tagttaaaca tggtctgcgt       660
     gccttaagag agacacttcc tgcagaacag gacctaacta caaagaatgt ttccattgga       720
     attgttggta aagacttgga gtttacaatc tatgatgatg atgatgtgtc tccattcctc       780
     gaaggtcttg aagaaaggcc acagagaaag gcacagcctg ctcaacctgc tgatgaacct       840
     gcagaaaagg ctgatgaacc aatggaacat taagtgataa gccagtctat atatgtatta       900
     tcaaatatgt aagaatatag gcaccacata ctgatgacaa taatctatac tttgaaccaa       960
     aagttgcaga gtggtggaat gctatgtttt aggaatcagt ccagatgtga gttttttcca      1020
     agcaacctca ctgaaaccta tataatggaa tacatttttc tttgagaggg tctgtataat      1080
     cattttctgg aaagtatggg tatctatact aatgttttta tatgaagaat ataggtgtct      1140
     ttgtggtttt aaagacaact gtgaaataaa attgtttcaa tgcctggtaa aaaaaaaaaa      1200
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa                               1237
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