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EBI DbfetchID AB179178; SV 1; linear; mRNA; STD; MAM; 1631 BP. XX AC AB179178; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA clone: QtsA-14162, similar to human DE mannosyl (alpha-1,6-)-glycoproteinbeta-1,2-N- acetylglucosaminyltransferase DE (MGAT2), mRNA, RefSeq: NM_002408.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1631 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (10-MAY-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, CC Tokyo, Japan; Division of Genetic Resources, National Institute CC of Infectious Diseases of Japan, Tokyo, Japan; National Health CC Research Institute, Taipei, Taiwan; Institute of Molecular Biology, CC Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, CC University of Chicago, Chicago, IL, USA, Center for Information CC Biology, CC National Institute of Genetics of Japan, Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert CC sequencing was done by primer-walking method using ABI DNA CC sequencer. XX FH Key Location/Qualifiers FH FT source 1..1631 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-14162" FT /db_xref="taxon:9541" FT CDS 321..1526 FT /note="Homo sapiens mannosyl FT (alpha-1,6-)-glycoproteinbeta-1,2-N- FT acetylglucosaminyltransferase (MGAT2), mRNA, RefSeq: FT NM_002408.2" FT /db_xref="GOA:Q4R3T7" FT /db_xref="InterPro:IPR007754" FT /db_xref="UniProtKB/TrEMBL:Q4R3T7" FT /protein_id="BAE02229.1" FT /translation="MRFRIYKRKVLILTLVVAACGFVLWSSNGRQRKNEALAPPLVDAE FT PVRGAGSRGGDHPSVAVGIRRVSNESAAPLVQVHNRPEYLRLLLDSLRKAQGIDNVLVI FT FSHDFWSTEINQLIAGVDFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLSKNAALKLG FT CINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF FT YHVFKKMWKLKQQECPECDVLSLGTYTASRSFYGMADKVDVKTWKSTEHNMGLALTRNA FT YQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQVPRIFHAGDCGMHHKKTC FT RPSTQSAQIESLLNNNKQYMFPETLTISEKFTMVAISPPRKNGGWGDIRDHELCKSYRR FT LQ" XX SQ Sequence 1631 BP; 402 A; 422 C; 440 G; 367 T; 0 other; agctgctggg tttcttccgg gacccgtggt gctgaatgga gaggaccgag acgaagccga 60 gccgcggctc ctagcggcgg cgccgatgcc cgagctgtag ctgccaggcg aggatgtgtg 120 gagcgcaggc ggcgcggggt agctgagagg tctcgggcct caggaccccc ggggtccggg 180 atgagttagc gagggcagcc gcgggggcca gttccgaccg tgacaggcca aggcgacggc 240 cgccgcccgc ccgccccatc cgtgcagaag cagctactcc tttccgcgcc cgcccgcccg 300 cgctcccggc cctggagacc atgaggttcc gcatctacaa acggaaggtg ctaatcctga 360 cgctcgtggt ggccgcctgc ggcttcgtcc tctggagcag caatgggcga caaaggaaga 420 acgaggccct cgccccgccg ttggtggacg ccgaacccgt gcggggtgcc ggcagccgtg 480 gcggggacca cccctctgtg gctgtgggca tccgcagggt ctccaacgag tcggcggctc 540 ccctggtcca ggtgcataac cggcccgaat acctcagact gctgctggac tcacttcgaa 600 aagcccaggg aattgacaac gtcctcgtca tctttagcca cgacttctgg tcgaccgaga 660 tcaatcagct gatcgccggg gtggatttct gtccggttct gcaggtgttc tttcctttca 720 gcattcagtt gtaccctaat gagtttccag gcagtgaccc tagagattgt cccagagacc 780 tgtcgaagaa tgccgctttg aaattggggt gcatcaatgc tgagtatccc gactccttcg 840 gccattatag agaggccaaa ttctcccaga ccaaacatca ctggtggtgg aagctgcatt 900 ttgtgtggga aagagtcaaa attcttcgag attatgctgg ccttatactt ttcctagaag 960 aagatcacta cttagcccca gacttttacc atgtcttcaa aaagatgtgg aaactgaagc 1020 agcaagagtg ccctgaatgt gatgttctct ccctggggac ctatactgcc agtcgcagtt 1080 tctatggcat ggctgacaag gtagatgtga aaacttggaa atccacagag cacaatatgg 1140 gtctagcctt gacccggaat gcctatcaga agctgatcga gtgcacagac actttctgta 1200 cttacgatga ttataactgg gactggactc ttcaatactt gactgtatct tgtcttccaa 1260 aattctggaa agtgctggtt cctcaagttc ctaggatttt tcatgctgga gactgtggta 1320 tgcatcacaa gaaaacctgt agaccatcca cccagagtgc ccaaattgag tcactcttaa 1380 ataataacaa acaatacatg tttccagaaa ctctaactat cagtgagaag tttactatgg 1440 tagccatttc tccacctaga aaaaatggag ggtggggaga tattagggac catgaactct 1500 gtaaaagtta tagaagactg cagtgaaaat cacaattaca gaagcaaaag tgacagtctt 1560 ctatttttga tatttgtcca aacaggacat acaattgaat aaaagagttt aggaactgga 1620 aaaaaaaaaa a 1631 // |
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