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EBI DbfetchID AB179151; SV 1; linear; mRNA; STD; MAM; 1608 BP. XX AC AB179151; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA clone: QtsA-13756, similar to human DE chromosome 9 open reading frame 98 (C9orf98), mRNA, RefSeq: NM_152572.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1608 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (10-MAY-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, CC Tokyo, Japan; Division of Genetic Resources, National Institute CC of Infectious Diseases of Japan, Tokyo, Japan; National Health CC Research Institute, Taipei, Taiwan; Institute of Molecular Biology, CC Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, CC University of Chicago, Chicago, IL, USA, Center for Information CC Biology, CC National Institute of Genetics of Japan, Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert CC sequencing was done by primer-walking method using ABI DNA CC sequencer. XX FH Key Location/Qualifiers FH FT source 1..1608 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-13756" FT /db_xref="taxon:9541" FT CDS 36..1475 FT /note="Homo sapiens chromosome 9 open reading frame 98 FT (C9orf98), mRNA, RefSeq: NM_152572.2" FT /db_xref="GOA:Q4R3W4" FT /db_xref="HSSP:1ZAK" FT /db_xref="InterPro:IPR000850" FT /db_xref="InterPro:IPR007862" FT /db_xref="UniProtKB/Swiss-Prot:Q4R3W4" FT /protein_id="BAE02202.1" FT /translation="MDSASVPHRIPPEMPQYGEENHIFELMQNMLEQLLIHQPEDPIPF FT MIQHLHKDNDNVPRIVILGPPASGKTTIAMWLCKHLNSSLLTLENLVLNEFSLTATKAK FT RLYLQRKTIPSALLVQLIQERLAEEDCVKRGWILDGIPETREQALRIQTLGITPRHVIV FT LSAPDTVLIERNLGKRIDPQTGEIYHTTFDWPPESEIQNRLMVPEDISELETAQKLLEY FT HRNIVRVIPSYPKILKVISADQPCVDVFYQALTYVQSNHRTNAPFTPRVLLLGPVGSGK FT SLQAALLAQKYRLVNVCCGQLLKEAMADRTTFGELIQPFFEKEMAVLDSLLMKVLSQRL FT DQQDCIQKGWVLHGVPRDLDQAHLLNSLGYNPNRVFFLNVPFDSIMERLTLRRIDPVTG FT ERYHLMYKPPPTMEILARLLQNPKDAEEQVKLKMDLSYRNSADLEQLYGSAITVNGDQD FT PYTVFEYIESGIINPLPKKIP" XX SQ Sequence 1608 BP; 416 A; 471 C; 414 G; 307 T; 0 other; gcggttacta gggagcggcg cgccgcgact gctgcatgga ctcggccagc gtcccgcacc 60 gtatcccccc cgagatgccc cagtacgggg aggagaacca catcttcgag ttgatgcaga 120 acatgctgga gcaactcctg atccatcagc ctgaggaccc catccccttc atgatccagc 180 acttgcataa agacaacgac aatgtgccca ggattgtaat attaggtcca cctgcctcgg 240 ggaaaacgac gatagcaatg tggctctgca aacatctgaa cagcagtctc ctcaccctgg 300 agaacctggt cttaaacgag ttttccctta cggccaccaa agccaaaagg ctttatctgc 360 aaaggaagac aattcccagc gcgctgctcg tccaactgat tcaggaacgc ctggctgaag 420 aggattgcgt caagcggggc tggattctgg atggcatccc cgagacgcgt gagcaggccc 480 tgaggatcca gaccctgggg atcaccccta gacacgtcat cgtgctgagt gctccagaca 540 cggtcctgat cgagagaaac ctggggaaga gaatcgaccc tcaaactgga gagatttatc 600 acaccacctt tgactggcca cccgaatctg aaatccagaa ccgtctgatg gtgccagagg 660 acatctcaga gctggagacg gctcagaaac tgctggagta tcataggaac atcgtcaggg 720 tcattccctc ctaccccaaa atcctcaaag tcatcagtgc tgaccagcca tgtgtggacg 780 tcttctacca ggctctgacc tatgtccaaa gcaaccatcg tactaatgcc ccgttcaccc 840 caagggtgct gctgctcggg cctgtgggca gtgggaaaag tctgcaggcc gccctcctgg 900 cccagaaata caggcttgtc aatgtctgct gtgggcaact gctgaaagag gctatggcag 960 acaggaccac gtttggcgag ctcattcagc ccttttttga aaaggagatg gcagttcttg 1020 acagcctcct catgaaggtg ctgagccagc gtctggacca gcaggactgc atccagaaag 1080 gctgggtgct gcacggcgtc ccacgggacc tggaccaggc gcacctgctg aatagcctgg 1140 gctacaatcc caacagggta tttttcctga atgtgccatt tgattccatc atggagcggc 1200 tgactctgag aagaattgat ccagtcactg gggagaggta ccacctcatg tacaagccac 1260 ctcccaccat ggagatcctg gctcgcctcc tgcagaaccc gaaggatgct gaagagcagg 1320 tcaagctgaa aatggacctg tcctacagga actcggccga cttggagcag ctctatgggt 1380 cggccatcac cgtcaacgga gaccaggacc catacaccgt ctttgaatac attgagagcg 1440 gcatcattaa tcccctgccc aagaaaatcc cctgatgggt tcagagccag gagcgccgcc 1500 ccagggaaag agttaatccc ctgcccccag ccccccaacc tctgcaaagc tcccctaaaa 1560 agccaataaa gcgtgctggg tacagtcaaa aaaaaaaaaa aaaaaaaa 1608 // |
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