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EBI DbfetchID AB179007; SV 1; linear; mRNA; STD; MAM; 1838 BP. XX AC AB179007; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA clone: QtsA-11376, similar to human DE UDP-N-acetylglucosamine:a-1,3-D-mannosidebeta-1,4-N- DE acetylglucosaminyltransferase IV-homolog(HGNT-IV-H), mRNA, RefSeq: DE NM_013244.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1838 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (10-MAY-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, CC Tokyo, Japan; Division of Genetic Resources, National Institute CC of Infectious Diseases of Japan, Tokyo, Japan; National Health CC Research Institute, Taipei, Taiwan; Institute of Molecular Biology, CC Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, CC University of Chicago, Chicago, IL, USA, Center for Information CC Biology, CC National Institute of Genetics of Japan, Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert CC sequencing was done by primer-walking method using ABI DNA CC sequencer. XX FH Key Location/Qualifiers FH FT source 1..1838 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-11376" FT /db_xref="taxon:9541" FT CDS 327..1763 FT /note="Homo sapiens FT UDP-N-acetylglucosamine:a-1,3-D-mannosidebeta-1,4-N- FT acetylglucosaminyltransferase IV-homolog(HGNT-IV-H), mRNA, FT RefSeq: NM_013244.2" FT /db_xref="GOA:Q4R4A8" FT /db_xref="InterPro:IPR006759" FT /db_xref="UniProtKB/Swiss-Prot:Q4R4A8" FT /protein_id="BAE02058.1" FT /translation="MFKFHQMKHIFEILDKMRCLRKRSTVSFLGVLVIFLLFMNLYIED FT SYVLEGDKQLIRETSTHQLNSERYVHTFKDLSNFSGAINVTYRYLAATPLQRKRYLTIG FT LSSVKRKKGNYLLETIKSIFEQSSYEELKEISVVVHLADFNSSWRDAMVQDITQKFAHH FT IIAGRLMVIHAPEEYYPILDGLKRNYNDPEDRVKFRSKQNVDYAFLLNFCANTSDYYVM FT LEDDVRCSKNFLTAIKKVIASLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFLLMFYQ FT EMPCDWLLTHFRGLLAQKNVIRFKPSLFQHMGYYSSYKGTENKLKDDDFEEESFDIPDN FT PPASLYTNMNVFENYEASKAYSSVDEYFWGKPPSTGDVFVIVFENPIIIKKIKVNTGTE FT DRQNDILHHGALDVGENVMPSKRRRQCSTYLRLGEFKNGNFEMSGVNQKIPFDIHCMRI FT YVTKTQKEWLIIRSISIWTS" XX SQ Sequence 1838 BP; 632 A; 311 C; 356 G; 539 T; 0 other; gagtgcatca agatctcttc tcaagggcgg atcatccctc ttctggttta gggatggaac 60 gtccaaaact agtttcccac ttccggttta ggggttgatg cttcaaaatt gtaatgaaca 120 gacaattgct tttggggtcc tagagtccct tgaaagttat gatggaaata ggagacctaa 180 agttcaagtg attgaatggc tgaagcagga aggaaaaacg tctgggagga cagactatca 240 tttggagcac tggctcagga gtctaaatca ccaagggctc atccatcaat caaccagaag 300 tgaagacaat agcttcaaag aagagaatgt ttaaatttca tcaaatgaaa catatttttg 360 aaatacttga taaaatgaga tgcctgcgaa aacgttctac agtgtcattc ttgggagttc 420 ttgtcatttt tctccttttt atgaacttgt acattgaaga tagctatgtt ctggaaggag 480 acaaacaact tataagggaa acatccacac atcaactgaa ttcagaacgc tatgttcata 540 cttttaagga tttgtctaat ttctcaggag ccataaatgt cacctatcgc tacctagctg 600 ccacaccttt acaaagaaag cggtatctta caattggact ttcttcagta aagcgaaaaa 660 aaggaaacta tttacttgag acaattaagt caatttttga gcagtccagt tacgaagaac 720 tgaaggaaat ttcagtggtg gttcacctag cagactttaa ttcttcctgg cgtgatgcca 780 tggtccagga tattacacag aaatttgcgc accatattat tgcaggaaga ttaatggtta 840 tacatgcccc agaggagtat tacccaatcc tggatggcct taaaagaaat tacaatgatc 900 cagaagatag agtcaaattt cgttcaaagc aaaatgtaga ttatgctttt ctgcttaatt 960 tttgtgccaa tacttctgac tattatgtaa tgcttgaaga tgatgttcga tgttcaaaaa 1020 atttcttaac tgccatcaag aaagtcattg catccctaga aggaacttac tgggtaactc 1080 ttgaattctc taagcttggc tacattggta aactctatca ttctcatgat ctcccacgtt 1140 tggcacattt tttattaatg ttttatcaag aaatgccttg tgattggcta ttgactcatt 1200 tccggggtct gttggctcag aaaaatgtga tccgttttaa accatctctc tttcagcaca 1260 tgggctatta ttcatcatac aaagggacag agaataagct aaaggatgat gattttgaag 1320 aggagtcatt tgacattcct gataaccccc ctgcaagtct gtacaccaac atgaatgtgt 1380 ttgaaaatta tgaagcaagc aaggcttaca gtagtgttga cgagtacttt tgggggaaac 1440 caccttcaac aggagatgtt tttgtgattg tatttgaaaa tccaattata attaaaaaaa 1500 ttaaagtaaa tactggaaca gaagatcgac aaaatgatat tttgcatcat ggagcactag 1560 atgttgggga aaacgttatg cctagcaaac gaaggagaca atgttctact tacttaagac 1620 taggagaatt caaaaatgga aactttgaaa tgtcaggtgt gaatcaaaaa attccatttg 1680 atatacattg tatgaggata tatgtcacca aaacacaaaa ggaatggcta attattagga 1740 gtattagcat ttggacttct tagccaatta aatcagtatg ttcagtttct gaaaaaaaaa 1800 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 1838 // |
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