 |
|
EBI DbfetchID AB169795; SV 1; linear; mRNA; STD; MAM; 1954 BP. XX AC AB169795; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis brain cDNA, clone: QccE-14020, similar to human DE seryl-tRNA synthetase (SARS), mRNA, RefSeq: NM_006513.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1954 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA, Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..1954 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QccE" FT /clone="QccE-14020" FT /db_xref="taxon:9541" FT CDS 151..1695 FT /note="Homo sapiens seryl-tRNA synthetase (SARS), mRNA, FT RefSeq: NM_006513.2" FT /db_xref="GOA:Q4R4U9" FT /db_xref="HSSP:1WLE" FT /db_xref="InterPro:IPR002314" FT /db_xref="InterPro:IPR002317" FT /db_xref="InterPro:IPR006195" FT /db_xref="InterPro:IPR010978" FT /db_xref="InterPro:IPR015866" FT /db_xref="InterPro:IPR018156" FT /db_xref="UniProtKB/Swiss-Prot:Q4R4U9" FT /protein_id="BAE01876.1" FT /translation="MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRR FT CRFRADNLNKLKNLCSKTIGEKMKKKEPVEDDESVPENVLNFDDLTADALANLKVSQIK FT KVRLLIDEAILKCDAERIKLEAERFENLREIGNLLHPSVPISNDEDADNKVERIWGDCT FT VRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQYALRTLGSRGYTP FT IYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALHR FT DEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEM FT FEEMITTAEEFYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCT FT DYQARRLRIRYGQTKKMMDKVEFVHMLNATMCATTRTICAILENYQTEKGITVPEKLKE FT FMPPGLQELIPFVKPAPIDQEPSKKQKKQHEGSKKKAAARDVALESRLQNMEVTDA" XX SQ Sequence 1954 BP; 509 A; 472 C; 557 G; 416 T; 0 other; ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg aattcctcga gcactgttgg 60 cctactgagc gcgccggcgc agtgccgcgg tcacaggctg agtgctgcgg cgcgatcctc 120 gcttacgtga gagttggccc gggaggaaag atggtgctgg atctggattt gtttcgggtg 180 gataaaggag gggacccagc cctcattcga gagacgcagg agaagcgctt caaggacccg 240 ggactagtgg accagctggt gaaggcagac agcgagtggc gacgatgtag atttcgggca 300 gacaacttga acaagctgaa gaacctatgc agcaagacga tcggagagaa aatgaagaaa 360 aaagagccag tggaagatga tgagtctgtc ccagagaacg tgttgaattt cgatgacctt 420 actgcagacg ctttagctaa cctgaaagtc tcacaaatca aaaaagtccg actcctcatt 480 gacgaagcca tcctgaagtg tgacgcagag cggataaagc tggaagcaga gcgatttgag 540 aacctccgag agattgggaa ccttctgcac ccttctgtgc ccatcagtaa cgatgaggat 600 gcggacaaca aagtagagag gatttggggt gattgtactg tcaggaagaa gtactctcat 660 gtggacctgg tggtgatggt agatggcttt gaaggcgaaa agggggccgt ggtggctggg 720 agtcgagggt acttcttgaa gggggtcctg gtgttcctgg aacaggctct catccagtat 780 gcccttcgca ccttgggaag tcggggctac actcccattt ataccccctt tttcatgaga 840 aaggaggtca tgcaggaggt ggcacagctc agccagtttg atgaagaact ttataaggtg 900 attggcaaag gcagtgaaaa gtctgatgac aactcctatg atgagaagta cctgattgcc 960 acctcagagc agcccatcgc tgccctgcac cgggatgagt ggctccggcc ggaggacctg 1020 cccatcaagt atgctggcct gtctacctgc ttccgtcagg aggtgggctc ccacggccgt 1080 gacacccgtg gcatcttccg agtccatcag tttgagaaga ttgaacagtt tgtgtactca 1140 tcaccacatg acaacaagtc gtgggagatg tttgaagaga tgattaccac cgcggaggaa 1200 ttctaccagt ccctgggcat tccttaccac atcgtgaata ttgtctcagg ttctttgaat 1260 catgctgcca gtaagaagct cgacctggag gcctggtttc cgggctcagg agccttccgt 1320 gagttggtct cctgttctaa ttgcacggat taccaggctc gccggcttcg aatccgatat 1380 gggcaaacca agaagatgat ggacaaggtg gagtttgtcc atatgctcaa tgctaccatg 1440 tgcgccacta cccgtaccat ctgcgccatc ctggagaact accagacaga gaagggcatc 1500 actgtgcctg agaaattgaa ggagttcatg ccgccaggac tgcaagaact gatccccttt 1560 gtgaagcctg cgcccattga ccaggagcca tccaagaagc agaagaagca acatgagggc 1620 agcaaaaaga aagcggcagc aagagacgtc gccctagaaa gcaggctgca gaacatggag 1680 gtcaccgatg cctgaacatt cctgcgtccc tgtttgcttt catttctgtc cgctgagatc 1740 tcagagcctg cccaacagca gggaaggcaa gcacccattc atcgccctgc ccccatctga 1800 ctgtggagct gagaggggag cagtgccgtg taccacacag atgttcctgt ctccttgcat 1860 gggcatagga acccatcact gatgactgat gaaaccatgt aataaagcat ctctggggag 1920 ggcttaggac tcttaaaaaa aaaaaaaaaa aaaa 1954 // |
|