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EBI DbfetchID AB169753; SV 1; linear; mRNA; STD; MAM; 2739 BP. XX AC AB169753; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis brain cDNA, clone: QnpA-18508, similar to human DE asparaginyl-tRNA synthetase (NARS), mRNA, RefSeq: NM_004539.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-2739 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA, Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..2739 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QnpA" FT /clone="QnpA-18508" FT /db_xref="taxon:9541" FT CDS 79..1755 FT /note="Homo sapiens asparaginyl-tRNA synthetase (NARS), FT mRNA, RefSeq: NM_004539.2" FT /db_xref="GOA:Q4R4Z1" FT /db_xref="HSSP:1X56" FT /db_xref="InterPro:IPR002312" FT /db_xref="InterPro:IPR004364" FT /db_xref="InterPro:IPR004365" FT /db_xref="InterPro:IPR004522" FT /db_xref="InterPro:IPR006195" FT /db_xref="InterPro:IPR012340" FT /db_xref="InterPro:IPR016027" FT /db_xref="InterPro:IPR018150" FT /db_xref="UniProtKB/Swiss-Prot:Q4R4Z1" FT /protein_id="BAE01834.1" FT /translation="MSLEVTRATAGMVLELYVSDREGSDATGDGTKEKPFKTGLKALMT FT VGKEPFPTIYVDSQKENERWNVISKSQLKNIKKMWHREQMKSESREKKEAEDSLRREKN FT LEEAKKITIKNDPALPEPKCVKISALEGYRGQRVKVFGWVHRLRRQGKNLMFLVLRDGT FT GYLQCVLADELCQCYNGVLLSTESSVAVYGMLNLTPKGKQAPGGHELSCDFWELIGLAP FT AGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMITRCFRDHFFDRGYHEITPP FT SLVQTQVEGGATLFKLNYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRR FT HLAEYTHVEAECPLLTFDDLLNRLEDLVCDVVDRILKSPAGSIVYELNPNFQPPKRPFK FT RMNYSDAIIWLKEHDIKKEDGTFYEFGEDIPEAPERLMTDTINEPILLCRFPVEIKSFY FT MQRCPEDSCLTESVDVLMPNVGEIVGGSMRTSDAEEILAGYKREGIDPAPYYWYTDQRK FT YGTCPHGGYGLGLERFLTWILNRYHIRDVCLYPRFVQRCTP" XX SQ Sequence 2739 BP; 830 A; 508 C; 642 G; 759 T; 0 other; gctctctgat gcaacgccgg agtcgcggaa gccgttggtg cacgtcggcg tggcaagacg 60 ttggcggtat tgcagtctat gtccttggag gtgaccaggg ccactgcagg catggtgcta 120 gagctgtacg tctctgaccg agagggaagc gatgccacgg gagatggaac caaggagaaa 180 ccatttaaga ctggtctaaa ggctttgatg acagtaggaa aagaaccatt tcctaccatt 240 tacgtagatt cacaaaaaga aaatgagagg tggaatgtta tttctaaatc acagttgaag 300 aacattaaaa agatgtggca tagggaacaa atgaagagtg aatcccggga aaagaaagag 360 gcagaagata gtttacgaag agaaaagaac ctggaagaag caaagaagat taccattaaa 420 aacgatccag ctctcccaga gccaaaatgt gtgaagatta gtgcattaga aggatataga 480 ggccaaagag taaaggtgtt tggctgggtc cacaggctgc gtaggcaagg aaagaattta 540 atgtttctgg tgttgcgaga tggtacaggt tatcttcagt gtgtcttggc ggatgagttg 600 tgtcagtgct acaatggagt attgttgtcc acagagagca gtgttgcagt gtatggaatg 660 ctaaatctta ccccaaaggg caagcaggct ccaggtggcc atgagctgag ttgtgacttc 720 tgggagctaa ttgggttggc ccctgctgga ggagctgaca acctgatcaa tgaggagtcc 780 gacgttgacg tccagctcaa caacagacac atgatgatcc gaggagaaaa catgtccaaa 840 atcctaaaag cgcgatccat gatcaccagg tgctttagag atcacttctt tgataggggg 900 taccatgaaa ttactcctcc atcattagtt caaacacaag tagaaggtgg tgccacactc 960 ttcaagctta actattttgg agaagaggca tttttgactc aatcctctca gttgtacttg 1020 gagacctgcc tcccagccct gggagatgtt ttttgtattg cgcagtcata ccgggcagag 1080 cagtccagaa cacgaaggca cctggctgag tacactcacg tggaagctga gtgtcctctc 1140 ctgacctttg atgacctcct gaaccggttg gaggacttgg tttgtgatgt ggtagatcga 1200 atattgaagt ctcctgcagg gagcatagtg tatgagctca acccgaactt tcagcccccc 1260 aaacggcctt tcaaacggat gaactattca gatgctatca tttggctaaa agaacatgat 1320 ataaagaaag aagatggaac tttctatgaa tttggagaag atatcccaga agctcctgag 1380 agactgatga cagacaccat taatgaacca atcttgctgt gtcgatttcc cgtggagatc 1440 aagtccttct acatgcagcg atgtcctgag gattcctgtc ttactgaatc tgtcgacgtg 1500 ttgatgccca atgttggtga gattgtggga ggctcaatgc gtaccagcga tgctgaagaa 1560 atactggcag gttataaaag ggaagggatt gaccctgctc cctattactg gtatacagat 1620 cagagaaaat atggcacgtg tccacatgga ggatatggct tgggcttgga acgattctta 1680 acatggattc tgaataggta tcacatccga gacgtgtgct tataccctcg atttgtccag 1740 cgctgcacgc cataaccagt ttctccagaa gtgtggagga aaggttgtga aaggaacagg 1800 ctctttaaaa aagaaaacaa aaagccagaa tcttcgtttt tctgtttcat tggggttttc 1860 tctgtctgtg tttctttcta ctaccataaa aagtacctca gatcacctga acatcgagtt 1920 aaggttatca tcgtaagaaa aaatatccat tataaagttt gggggaaata cctggcatga 1980 aactgtagtt agggatacat tccagcattt tattcacttt attcaagtta ttcattttat 2040 tcaagttata tgtatatata attcaacaat tttagattat ggtgtaagat attccggtaa 2100 cttaatctat ctgtgctttt aagtgtacct ggaattcttt gatttatttt attgcattaa 2160 tgagttaaag caaaaatctt gggggaagaa attggcaata tggtataaaa atctcatatt 2220 agaacacagt ataattcagc agcagacact agaatcaaat gatagtcttt tgtatcagtt 2280 attaatcttt tctaagtctg catagctgct tataattctg aggcatacaa attgatatgg 2340 aagaatttgt aaaaacagaa ttgccttaaa ggattttaag taagagcata attttgggga 2400 gagttcttta gtgattcaca atatccctct tagcattagt ttaaggtaaa gaggcagact 2460 gatgttccct ctttcctggt aattcctgag taattaagag taagttccaa aagaatttgt 2520 agctggaatc ttaataacaa ttgtcggtgg ctgtttgagt tgcccccacc atgtccttag 2580 atctaatctg tgctacctta ttaactcaca gcaggcttac tgaatggctt catttcagat 2640 ttagttgatt tctccaccaa atgcatgtca tgtattctca ataggctgta ttcccagcag 2700 tcaataaaag aacatccgta aaaaaaaaaa aaaaaaaaa 2739 // |
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