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EBI DbfetchID AB169713; SV 1; linear; mRNA; STD; MAM; 2046 BP. XX AC AB169713; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis brain cDNA, clone: QccE-21944, similar to human DE pyrroline-5-carboxylate reductase-like (PYCRL), mRNA, RefSeq: NM_023078.1. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-2046 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA, Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..2046 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QccE" FT /clone="QccE-21944" FT /db_xref="taxon:9541" FT CDS 43..867 FT /note="Homo sapiens pyrroline-5-carboxylate reductase-like FT (PYCRL), mRNA, RefSeq: NM_023078.1" FT /db_xref="GOA:Q4R531" FT /db_xref="HSSP:2AMF" FT /db_xref="InterPro:IPR000304" FT /db_xref="InterPro:IPR004455" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q4R531" FT /protein_id="BAE01794.1" FT /translation="MAAAGSATRRVGFVGAGRMAGAIAQGLIRAGKVEAQRIVASAPTD FT RNLCHFQALGCQTTHSNQEVLQSCLLVIFATKPHILPAVVAEVAPVVTAEHILVSVAAG FT VSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETKLLQHLLEACGRCE FT EVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAIKMGMPSSLAHRIAAQTLLGTAKMLLH FT EGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRARELSRK" XX SQ Sequence 2046 BP; 379 A; 652 C; 645 G; 370 T; 0 other; agtagagtgc ggacaaaaag ccggccggag cggggcgaca agatggcggc tgcggggtcg 60 gctacgcggc gcgtgggctt cgtgggcgcg ggccgcatgg cgggggccat cgcgcagggc 120 ctcatcagag cagggaaagt ggaagctcag cgcatagtgg ccagtgcacc aacagacagg 180 aacttatgtc actttcaagc tctgggttgc cagaccaccc actccaacca ggaggtgctg 240 cagagctgcc tgctcgtcat ctttgccacc aagccccaca tcctgccagc tgtggtggca 300 gaggtggctc ctgtggtcac tgctgaacac atcttggtgt ccgtggctgc tggggtgtct 360 ctgagcaccc tggaggagct gctgccccca aacacacggg tgctgcgggt cttgcccaac 420 ctgccctgtg tggtccagga aggggccata gtgatggcgc ggggccgcca cgtggggagc 480 agcgagacca agctcctgca gcatctgctg gaggcctgtg ggcggtgtga ggaggtgcct 540 gaagcctacg tcgacatcca cactggcctc agtggcagtg gcgtggcctt cgtgtgtgca 600 ttctctgagg ccctggctga aggcgccatc aagatgggca tgcccagcag cctggcccac 660 cgcatcgctg cccagaccct gctggggaca gccaagatgc tgctgcacga gggccaacac 720 ccagcccagc tgcgctcaga tgtgtgcacc ccgggtggca ccaccatcta cgggctccat 780 gccctggagc agggcgggct gcgagcagcc accatgagcg ctgtggaggc cgccacctgc 840 cgggccaggg agctcagcag aaagtaggct gggttctggc catccttgcc tgccttcgtg 900 cccccacctc tccctgtgcc ccttcccctg aggactgtgg ctccctccct cctgcacgag 960 ggtctcctcc ctcctgcacg agccatatgg ggtctacaca agagaccctc tcccctacag 1020 taaagccagg ggccctggcc tgctgtgggt ggggccccca cggggagctg gagcctgccc 1080 tgcagcccag agaagcgggt gcctgtggtg ggtgtgctcg tcctctgcta aagaggagga 1140 actgctgggc caggtcttca gcagtgctgg ggtggcacca ggcgggcagt ggtaggtggg 1200 gcagcttgag gtctgagagg gtggccctgg ccagccagga caaatgcaga cccctggctt 1260 tagttgggat acagtctccc tctttcctcc tgatcctaaa ctcctgacaa gtggccaggt 1320 ggctctgggc cctcctgccc ctcacctagg tcaggggtcc tggaatgccc cgtggctctg 1380 gcaccaccac actggcctct gacggtagga cttgtcccct ccacatgtcc ctaaaaggca 1440 gcaagtcagg aaagccagga cttgggggag aagcaaagct ccaggattga agtctagggt 1500 tctagggcct ttgggtgggg agggcccagg ccaggcagtc ttagctccat cccctgcccc 1560 acaagattca cctgggcctc cagtcccatg ctggccccaa ctgctgcagc gctcagcttc 1620 cacccagcag cctctggagg aaggcgggga gcatgccctc agcgaggctg ggcagcgggt 1680 tctgctgtgc catctccctg tgtgcctggg cagatcaatc ccccagtgca aaacagggct 1740 aagggcacct gcaggacagc agcacgctcc atccttcatg ctcagctgcc tctgtggcca 1800 cagacttctg cccgccacct gctctctctt actcccccga gcctagccca tccgtaagct 1860 ccctcccccg cctggttccc ggggcaggct gactcagtcg actgcttggt ccaagcctgg 1920 ccctggcact tgtcagggtc agcctaagga gatgggaata aagatgccgg agagcaccaa 1980 gtgagcttat gtttcaccag aggtgaataa aaccaagttc cgggccagga aaaaaaaaaa 2040 aaaaaa 2046 // |
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