spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB169680; SV 1; linear; mRNA; STD; MAM; 2456 BP.
XX
AC   AB169680;
XX
DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
XX
DE   Macaca fascicularis brain cDNA, clone: QccE-17236, similar to human
DE   galactosidase, beta 1 (GLB1), transcript variant 179423,mRNA, RefSeq:
DE   NM_000404.1.
XX
KW   fis (full insert sequence); FLI_CDNA; oligo capping.
XX
OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
XX
RN   [1]
RP   1-2456
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
XX
RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
XX
RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
XX
CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of:Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA, Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2456
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QccE"
FT                   /clone="QccE-17236"
FT                   /db_xref="taxon:9541"
FT   CDS             94..2142
FT                   /note="Homo sapiens galactosidase, beta 1 (GLB1),
FT                   transcript variant 179423,mRNA, RefSeq: NM_000404.1"
FT                   /db_xref="GOA:Q60HF6"
FT                   /db_xref="InterPro:IPR001944"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="InterPro:IPR017853"
FT                   /db_xref="InterPro:IPR019801"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q60HF6"
FT                   /protein_id="BAE01761.1"
FT                   /translation="MPGFLVRILPLLLPLLLLGPTRGLRNATRRVFEIAYSQDRFLKDG
FT                   QPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNTIQTYVPWNFHEPWPGQYQFSEDHDV
FT                   EYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKEAILLRSSDPDYLAAVDKWLG
FT                   VLLPKMKPLLYQNGGPIITVQVENEYGSYFACDFDYLRFLQKRFHHHLGDDVVLFTTDG
FT                   AHETFLQCGALQGLYTTVDFGPGSNITDAFQIQRKCEPKGPLINSEFYTGWLDHWGQPH
FT                   STIKTEVVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEA
FT                   GDLTEKYFALRNVIQKFEKVPEGPIPPSTPKFAYGKVSLEKLKTVGAALDILCPSGPIK
FT                   SLYPLTFIQVKQYYGFVLYRTTLPQDCSNSTPLSSPFNGVHDRAYVAVDGIPQGVLERN
FT                   RVITLNITGKTGATLDLLVENMGRVNYGAYINDFKGLVSNLTLDSNILTGWTIFPLDTE
FT                   DAVRSHLGGWEHRDSGRHDEAWAHSSSNYTLPAFYVGNFSIPSGIPDLPQDTFIQFPGW
FT                   TKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELERAPCSSDGPELCA
FT                   VEFVDRPVIGSSQIYDHLSKPVEQRLMAPPPKKTKIRGWSMYDDESL"
XX
SQ   Sequence 2456 BP; 571 A; 654 C; 642 G; 589 T; 0 other;
     ggacgagggg cggtgccagg ccgcgggtcc ttagtcaagt gacgcggagc ggccggcctg        60
     ggcgccgact gcagagccgg gaggctggtg gtcatgccgg ggttcctggt tcgcatcctc       120
     cctctgctgc tgcctctgct gcttctgggc cctacgcgcg gcttgcggaa tgccacccgg       180
     agggtgtttg aaattgccta tagccaagac cgcttcctca aggatggcca gccgtttcgc       240
     tacatctcag gaagcattca ctactcccgc gtcccccgct tctactggaa ggaccggctg       300
     ctgaagatga agatggctgg gctgaacacc atccagacgt atgtgccctg gaactttcat       360
     gagccctggc caggacagta ccagttttct gaggaccatg atgtggaata ttttcttcgg       420
     ctggctcacg agctgggact gctggttatc ctgaggcccg gaccctacat ctgtgcggag       480
     tgggaaatgg gaggattacc tgcttggctg ctagagaaag aggctattct tctccgctct       540
     tccgacccag attacctggc agctgtggac aagtggttgg gagtccttct gcccaagatg       600
     aagcctctcc tctatcagaa tggagggcca attataacag tgcaggttga aaatgaatat       660
     ggcagctact tcgcctgtga ttttgactac ctgcgcttcc tgcagaagcg ctttcaccac       720
     catctggggg atgatgtggt tctgttcacc acggacggcg cacatgagac attcctgcaa       780
     tgtggggccc tgcagggcct ctacaccacg gtggactttg gaccaggcag caacatcaca       840
     gatgctttcc aaatccagag gaagtgtgag cccaaaggac ccttgatcaa ttctgaattc       900
     tatactggct ggctagacca ctggggccaa cctcactcca caatcaagac tgaagtggtg       960
     gcttcctccc tgtatgatat acttgcccgt ggggcgagtg tgaacttgta catgtttata      1020
     ggtgggacca attttgccta ttggaatggg gccaactcac cctatgcagc ccagcccacc      1080
     agctacgact atgatgcccc actgagtgag gccggggacc tcactgagaa gtattttgct      1140
     ctgcgaaacg ttatccagaa gtttgaaaaa gtaccagaag gtcctatccc tccatctaca      1200
     ccaaagtttg catatggaaa ggtctctctg gaaaagttaa agacagtggg agcagctctg      1260
     gacattctgt gcccctctgg gcccatcaaa agcctttatc ccttgacatt tatccaggtg      1320
     aaacagtatt atgggtttgt gctataccgg acaacacttc ctcaagattg cagcaactca      1380
     acacctctct cttcaccctt caatggagtc cacgatcgag cgtatgttgc tgtggatggg      1440
     atcccccagg gagtccttga gcgaaacagg gtgatcactc tgaacataac agggaaaact      1500
     ggagccactc tggaccttct ggtagagaat atgggacgtg tgaactatgg cgcatatatc      1560
     aacgatttta agggtttggt ttctaacctg accctcgatt ccaatatcct caccggctgg      1620
     acgatcttcc cactggacac tgaggatgca gtgcgcagcc acctcggggg ctgggaacac      1680
     cgtgacagtg gccgccacga tgaagcctgg gcccacagct catccaacta cacgctcccg      1740
     gccttttatg tggggaactt ctccattccc agtgggatcc cagacttgcc ccaggacacc      1800
     tttatccagt ttcctggatg gaccaagggc caggtctgga ttaatggctt taaccttggc      1860
     cgctattggc cagcccgggg ccctcagttg accttgtttg tgccccagca catcctgatg      1920
     acctcagccc caaacaccat caccgtgctg gaactggagc gcgcaccctg cagcagtgat      1980
     ggtccagaac tgtgtgctgt ggagttcgtg gacaggccag ttattggctc atctcagatc      2040
     tacgatcatc tctccaaacc tgttgagcaa agactcatgg ccccaccccc caaaaaaaca      2100
     aagattcgtg gctggagcat gtatgatgat gaaagcctgt gactttgagg gattctactc      2160
     tgaatgtacc tcccagatcc tccctgtcat gccacattgc actgattgga gtgtggaatt      2220
     ggaaaggaat ttcggatgtg gattttcacc tgaggtttcc ctgcatccct gcagtgccaa      2280
     agccccacct tcagggacca cctggaatgt gtgaggggct aacagcacag taacgtgcat      2340
     acatatctgc agggttggaa tgggagcttt aaaggtggta gtgatttgta ttttggaaga      2400
     atcatgttgc ctttttgtta aataaaatct gtactcaaaa aaaaaaaaaa aaaaaa          2456
//
spacer
spacer