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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB169663; SV 1; linear; mRNA; STD; MAM; 1847 BP.
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AC   AB169663;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
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DE   Macaca fascicularis brain cDNA, clone: QccE-15256, similar to human
DE   1-acylglycerol-3-phosphate O-acyltransferase 4(lysophosphatidic acid
DE   acyltransferase, delta) (AGPAT4),mRNA, RefSeq: NM_020133.1.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-1847
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of:Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA, Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1847
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QccE"
FT                   /clone="QccE-15256"
FT                   /db_xref="taxon:9541"
FT   CDS             214..1350
FT                   /note="Homo sapiens 1-acylglycerol-3-phosphate
FT                   O-acyltransferase 4(lysophosphatidic acid acyltransferase,
FT                   delta) (AGPAT4),mRNA, RefSeq: NM_020133.1"
FT                   /db_xref="GOA:Q4R581"
FT                   /db_xref="InterPro:IPR002123"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q4R581"
FT                   /protein_id="BAE01744.1"
FT                   /translation="MDLAGLLKSQFLCHLVFCYVFIASGLIINTVQLFTLLLWPINKQL
FT                   FRKINCRLSYCISSQLVMLLEWWSGTECTIFTDPRAYPKYGKENAIVVLNHKFEIDFLC
FT                   GWSLSERFGLLGGSKVLAKKELAYVPIIGWMWYFTEMVFCSRKWEQDRKTVATSLQHLR
FT                   DYPEKYFFLIHCEGTRFTEKKHEISMQVARAKGLPRLKHHPLPRTKGFAITVRSLRNVV
FT                   SAVYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRIPLEDIPEDDDRCSAWLHKLYQEK
FT                   DAFQEEYYRTGTFPETPMVPPRRPWTLVNWLFWASLVLYPFFQFLVSMIRSGSSLTLAS
FT                   FILVFFVASMGVRWMIGVTEIDKGSAYGNSDSKQKQND"
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SQ   Sequence 1847 BP; 428 A; 449 C; 502 G; 468 T; 0 other;
     gctttttctt tccagtgttg gctgacttac agctcttata aactagtggc aatttctgaa        60
     cccagccggc tccgtctcag cttctggttt ctaagtccat gtgccaaagg ctgccaggaa       120
     ggagacgcct tcctgagtcc tggatctttc ttccttctgg aaatctttga ctgtgggtat       180
     tgatttattt ctgaataaga gcgtccacac atcatggacc tcgcgggact gctgaagtct       240
     cagttcctgt gccacctggt cttctgctac gtcttcattg cctcggggct aatcatcaac       300
     accgttcagc tcttcactct cctcctctgg ccaattaaca agcagctttt ccggaagatc       360
     aactgcagac tgtcctattg catctcaagc cagctggtga tgctgctgga gtggtggtcg       420
     ggcacggaat gcaccatctt cacggacccg cgcgcctacc ccaagtatgg gaaggaaaat       480
     gccatcgtgg ttctcaacca caagtttgaa attgactttc tctgtggctg gagcctgtcc       540
     gaacgctttg ggctcttagg gggctccaag gtcctggcca agaaagagct ggcctacgtt       600
     ccaattatcg gctggatgtg gtacttcacc gagatggtct tctgttcgcg caagtgggag       660
     caggatcgca agacggttgc caccagtttg cagcacctcc gggactaccc ggagaaatat       720
     tttttcctga tccactgtga gggcacgcgg ttcacggaga agaaacatga gatcagcatg       780
     caagtggccc gggccaaggg gctgcctcgc ctcaagcacc acccgttgcc gcgaaccaag       840
     ggcttcgcca tcaccgtgag gagcttgaga aatgtagttt cagctgtata tgactgtaca       900
     ctcaatttca gaaataatga aaatccaaca ctgctgggag tcctaaacgg aaagaaatac       960
     catgcagatt tgtatgttag gaggatccca ctggaagaca tccctgaaga cgatgacagg      1020
     tgctcggcct ggctgcacaa gctctaccag gagaaggatg cctttcagga ggagtactac      1080
     aggacgggca ccttcccaga gacgcccatg gtgccccccc ggcggccctg gaccctcgtg      1140
     aactggctgt tttgggcctc gctggtgctc taccctttct tccagttcct ggtcagcatg      1200
     atcaggagcg ggtcttcctt gacgctggcc agcttcatcc ttgtcttctt cgtggcctcc      1260
     atgggagttc gatggatgat tggtgtaacg gaaattgaca agggctctgc ctacggcaac      1320
     tctgacagca agcagaaaca gaatgactga ctcagggagg tgtcaccatc cgaagggaac      1380
     cttggggaac tggtggcctc tgcgtatcct cctcagtggg acatggtgac aaaggctggg      1440
     tgagccctgc tgggcacggc ggaagtcacg gcctctccag ccagggagtc tggtctgaag      1500
     gccggatggg gaggaagatg ttttgtaatc tttttttccc catgtgcttt agtgggcttt      1560
     ggttttttct ttttgtgcga gtgtgtgtga gaatggctgt gtggtgagtg tgaactttat      1620
     tctgtgatca tggaaagggt attttaggct gcaggggagg gcagggctgg ggaccgaagg      1680
     ggacaagttc ccctttcatc ctttggtgct gagttttctg taacccttgg ttgccagaga      1740
     taaagtgaaa agtgctttag gtgagatgac taaattatgc ctccaaaaaa aaaaaaaaaa      1800
     aaaaattaaa gtgcttgtct gggtaaaaaa aaaaaaaaaa aaaaaaa                    1847
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