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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB169611; SV 1; linear; mRNA; STD; MAM; 2025 BP.
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AC   AB169611;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
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DE   Macaca fascicularis brain cDNA, clone: QnpA-20646, similar to human
DE   UDP-GlcNAc:betaGalbeta-1,3-N-acetylglucosaminyltransferase 6 (B3GNT6),
DE   mRNA, RefSeq: NM_006876.1.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-2025
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of:Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA, Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..2025
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QnpA"
FT                   /clone="QnpA-20646"
FT                   /db_xref="taxon:9541"
FT   CDS             88..1335
FT                   /note="Homo sapiens UDP-GlcNAc:betaGalbeta-1,3-N-
FT                   acetylglucosaminyltransferase 6 (B3GNT6), mRNA, RefSeq:
FT                   NM_006876.1"
FT                   /db_xref="GOA:Q4R5D3"
FT                   /db_xref="UniProtKB/TrEMBL:Q4R5D3"
FT                   /protein_id="BAE01692.1"
FT                   /translation="MQMSYAIRCAFYQLLLAALMLVAMLQLLYLSLLSGLHGQEEQDQY
FT                   FEFFPPSPRSVDQVKTQLRTALASGGVLDASGDYRVYRGLLKTTMEPNDVILATHASVD
FT                   NLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLAYALSSHCPDMRARVAMHLVCPSRY
FT                   EAAVPDPREPGEFALLRSCQEVFDKLARVAQPGINYALGTNVSYPNNLLRNLAREGANY
FT                   ALVIDVDMVPSEGLWRGLREMLDQSNQWGGTALVVPAFEIRRARRMPMNKNELVQLYQV
FT                   GEVRPFYYGLCTPCQAPTNYSRWVNLPEESLLRPAYVVPWQDPWEPFYVAGGKVPTFDE
FT                   RFRQYGFNRISQACELHVAGFDFEVLDEGFLVHKGFKEALKFHPQKEAENQHNKILYRQ
FT                   FKQELKAKYPNSPRRC"
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SQ   Sequence 2025 BP; 439 A; 545 C; 594 G; 447 T; 0 other;
     ggcaggctgc ggtaaatccg ggcttgcggc cgctggtgta gtctgtggcc gggtggtcgt        60
     tgctgcgcgc cccgagcccc gacagccatg cagatgtcct acgccatcag gtgcgccttc       120
     taccagctgc tgctggccgc gctcatgctg gtggcgatgc tgcagctgct ctacctgtcg       180
     ctgctgtccg gactgcacgg acaggaggag caagaccaat attttgagtt ctttcccccg       240
     tccccacggt ccgtggacca ggtcaagacg cagcttcgca ccgcgctggc ctccggaggc       300
     gtactggacg ccagcggcga ttaccgcgtc tacaggggcc tgctgaagac caccatggaa       360
     cccaacgatg tgatcctggc cacgcacgcc agcgtggaca acctgctgca cctgtcgggt       420
     ctgctggagc gctgggaggg cccactgtcc gtgtcggtgt tcgcggccac caaggaggag       480
     gcgcagctgg ccacggtgct ggcctacgcg ctgagcagcc actgccccga catgcgcgcc       540
     agggtcgcca tgcacctcgt gtgcccctct cgttacgagg cagccgtgcc cgacccccgg       600
     gagccggggg agtttgccct gctgcggtcc tgccaggagg tctttgacaa gctagccagg       660
     gtggcccagc ccgggattaa ttatgcgttg ggcaccaatg tctcctaccc caataatctg       720
     ctgaggaatc tggctcgtga gggggccaac tatgccctgg tgatcgatgt ggacatggtg       780
     cccagcgagg ggctgtggag aggcctgcgg gaaatgctgg atcagagcaa ccagtggggt       840
     ggcaccgcgc tggtggtgcc tgccttcgag atccgacgag cccgccgtat gcccatgaac       900
     aaaaacgagc tggtgcagct ctaccaggtt ggcgaggtgc gacccttcta ttatgggttg       960
     tgcacaccct gccaggcacc caccaactat tcccgctggg tcaacctgcc ggaagagagc      1020
     ttgctgcggc ctgcctacgt ggtaccttgg caggacccct gggagccatt ctacgtggca      1080
     ggaggcaagg tgcccacctt cgacgagcgc tttcggcagt acggcttcaa cagaatcagc      1140
     caggcctgcg agctgcatgt ggcggggttt gattttgagg tcctggacga aggtttcttg      1200
     gttcataagg gcttcaaaga agcgttgaag ttccatcccc aaaaggaggc cgaaaatcag      1260
     cacaataaga tcctttatcg ccagttcaaa caggagttga aggccaagta ccccaactct      1320
     ccccgacgct gctgagccct tccctaccct agtctgagaa gtcagcctct gggctcctca      1380
     ggccaccgtt taggcctgac tggggtaaaa aatgtcgctc cactttacag aggtagctgt      1440
     ggtgttgaaa cactggactt gaatatgggg tgctgggatc gagtcctagc tttaccacta      1500
     actagctgtg tggccttgag taaatcccgt tacctctctg agcctcggtt accctgtctg      1560
     taaaaaggga ggtgagaata cctacctcac ggaactgtcg ggaggctcag atgagatgtt      1620
     gtatgtgaaa acattctgta agcttcgtac aaatgtgaag tattaatatt atcgcagtat      1680
     tattattgtt attattattg ttattattaa caatcttggg tgggtagtag gagagcaaaa      1740
     agtatgaatg ggatggagct aagaagtctg agtacttaat gaaatggact ttttggaaag      1800
     aaatcagatg aaggcataaa ctttagttct tagctcttga acagaagcct aaaattcttg      1860
     gttctctcag gccttcgccc tcaagggctc tagaggaggg aagactctgc aggctccatg      1920
     ggtgacagcc tgagatctgt cccttcaaca ggctgggctg ggtatgtgcc taccgatgac      1980
     aatgtgtaaa taaatgcgtg ttcacaccca aaaaaaaaaa aaaaa                      2025
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