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EBI DbfetchID AB169600; SV 1; linear; mRNA; STD; MAM; 2379 BP. XX AC AB169600; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis brain cDNA, clone: QnpA-16101, similar to human DE phosphoglucomutase 1 (PGM1), mRNA, RefSeq: NM_002633.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-2379 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA, Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..2379 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QnpA" FT /clone="QnpA-16101" FT /db_xref="taxon:9541" FT CDS 108..1796 FT /note="Homo sapiens phosphoglucomutase 1 (PGM1), mRNA, FT RefSeq: NM_002633.2" FT /db_xref="GOA:Q4R5E4" FT /db_xref="InterPro:IPR005841" FT /db_xref="UniProtKB/Swiss-Prot:Q4R5E4" FT /protein_id="BAE01681.1" FT /translation="MVKIVTVKTQAYPDQKPGTSGLRKRVKVFQSSANYAENFIQSIIS FT TVEPAQRQEATLVVGGDGRFYMKEAIQLIARIAAANGIGRLVIGQNGILSTPAVSCIIR FT KIKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAICPDL FT KVDLGVLGKQQFDLENKFEPFTVEIVDSVEAYATMLRNIFDFSALKELLSGPNRLKIRI FT DAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEH FT DFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGA FT LDRVANATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWL FT SILATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQ FT FSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIIFRLSGTGSAGATIRLY FT IDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRSAPTVIT" XX SQ Sequence 2379 BP; 605 A; 594 C; 618 G; 562 T; 0 other; agcccccacc cgccgcgccc tcaccccaga gcagctgcag ccacagccgc ggccgcccct 60 ccgccagccg agtcagcagc tctgaccccc ggcagcaagt cgccaccatg gtgaagatcg 120 tgacagtgaa gacccaggcg tacccggacc agaagccggg cacgagcggg ctgcggaagc 180 gggtgaaggt gttccagagc agcgccaact acgcggagaa cttcatccag agtatcatct 240 ccaccgtgga gccggcgcag cggcaggagg ccacgctggt ggtgggcggg gacggacggt 300 tctacatgaa ggaggccatc cagctcatcg cccgcatcgc cgccgccaac gggattggtc 360 gcttggttat tggacagaat ggaatcctct ccacccctgc tgtatcctgc atcattagaa 420 aaatcaaagc cattggtggg atcattctga cagccagcca caacccaggg ggccccaatg 480 gagattttgg aatcaaattc aatatttcta atggaggtcc tgctccagaa gcaataactg 540 ataaaatttt ccaaatcagc aagacaattg aagaatatgc aatttgccct gacctgaaag 600 tagaccttgg tgttctcgga aagcagcagt ttgacttgga aaataagttc gaacccttca 660 cagtggaaat tgtggattcg gtggaagcat atgctacaat gctgagaaac atctttgatt 720 tcagtgcact gaaagaacta ctttctgggc caaaccgact gaagatccgt attgatgcta 780 tgcatggagt tgtgggaccg tatgtaaaga agatcctctg tgaagaactc ggtgcccctg 840 caaactcagc agttaactgc gttcctctgg aggactttgg aggccaccac cctgacccca 900 acctcaccta tgcagctgac ctggtggaga ccatgaagtc aggagagcat gattttgggg 960 ctgcctttga tggagatggg gatcgaaaca tgattctggg caagcatggg ttctttgtga 1020 acccttcaga ctctgtggct gtcattgctg ccaacatctt cagcattccg tatttccagc 1080 agaccggggt ccgtggcttt gcacggagca tgcccacgag tggtgccctg gaccgggtgg 1140 ctaatgctac aaagatcgct ttgtatgaga ccccaactgg ctggaagttt tttgggaatt 1200 tgatggatgc gagcaaactg tccctttgtg gggaggagag cttcgggact ggttctgacc 1260 acatccgtga gaaagatgga ctgtgggctg tccttgcctg gctctccatc ctagccaccc 1320 gcaagcagag tgtggaggac attctcaaag atcattggca aaagtatggc cggaatttct 1380 tcaccaggta tgattacgag gaggtggaag ctgagggtgc aaacaaaatg atgaaggact 1440 tggaggccct gatgtttgat cgctcctttg tggggaagca gttctcagca aatgacaaag 1500 tttacaccgt ggagaaggcc gataactttg aatacagcga ccccgtggat ggaagcattt 1560 caagaaatca gggcttgcgg ctcattttca cagatggttc tcgaatcatc ttccgactga 1620 gcggcactgg gagtgcgggg gccaccattc ggctgtacat tgatagctat gagaaggatg 1680 ttgctaagat taaccaggac cctcaggtca tgttggcccc ccttatttcc attgctctca 1740 aagtgtccca gctgcaggag aggacgggac gcagtgcacc caccgtcatc acctaagaag 1800 acaggccaga tgtggtacgt ccctccaccc ccggacccat ccaagtcatc tgattgaaga 1860 gcatgacaga aacaaaatgt attcaccaag ctttttagga tttgactttt ccactaacca 1920 gttgacgagc agtgcatttg caaggcactg ccaaagaaga tgcccttggg agctatgagg 1980 gaaagaggac ctgctggctt agatcaatct caattccttt tcatgccctt ctgcattgct 2040 gctgtgtggg tatttgtctc cttagcatca ggtacagttt acactacaat gtaaggtgca 2100 gatggagcat cagcagcgag caaggccatt cttcatcctt aggatgtggc aatgaaatga 2160 cggtgcaaat tcctttctct ttcgtgaatc tttcccccca tttcctgttt acatgtaacc 2220 caacaaaatg caatttctgg tgccttctgt ccaatcagtt ttttcctctg agtgagacgt 2280 acttggctac agatttctgc cttgttttgc gacattgtcc cattcgcaca gatattttgg 2340 gataataaag gaaaataagc tacaaaaaaa aaaaaaaaa 2379 // |
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