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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB169532; SV 1; linear; mRNA; STD; MAM; 2053 BP.
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AC   AB169532;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
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DE   Macaca fascicularis brain cDNA, clone: QccE-14568, similar to human
DE   glutamic-oxaloacetic transaminase 1, soluble (aspartateaminotransferase 1)
DE   (GOT1), mRNA, RefSeq: NM_002079.1.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-2053
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of:Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA, Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..2053
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QccE"
FT                   /clone="QccE-14568"
FT                   /db_xref="taxon:9541"
FT   CDS             127..1368
FT                   /note="Homo sapiens glutamic-oxaloacetic transaminase 1,
FT                   soluble (aspartateaminotransferase 1) (GOT1), mRNA, RefSeq:
FT                   NM_002079.1"
FT                   /db_xref="GOA:Q4R5L1"
FT                   /db_xref="InterPro:IPR015421"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q4R5L1"
FT                   /protein_id="BAE01614.1"
FT                   /translation="MAPPSVFSEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDD
FT                   CHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGV
FT                   QSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWD
FT                   AEKRGLDLQGFLNDLENAPEFSIIVLHACAHNPTGTDPTPEQWKQIASVMKHRFLFPFF
FT                   DSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESI
FT                   LRVLSQMEKIVRITWSNPPAQGARIVADTLSNPELFEEWKGNVKTMADRILTMRSELRA
FT                   RLEALKTPGTWNHITDQIGMFSYTGLNPKQVEYLINEKHIYLLPSGRINVSGLTTKNLD
FT                   YVATSIHEAITKIQ"
XX
SQ   Sequence 2053 BP; 520 A; 509 C; 520 G; 504 T; 0 other;
     attggaggcc gcgggcccgc ccccgtcggc taggtgaagg tgagagtctc ctccagtcgc        60
     agcggccaga cctgacaggc cagctccggg cgcagggtga aagcgttcgg ttcctgctct       120
     ctcgatatgg cgcctccgtc agtcttttcc gaggttccgc aggcccagcc tgtcctggtc       180
     ttcaagctca ctgccgactt ccgggaggat ccggaccccc gcaaggtcaa cctgggagtg       240
     ggagcatatc gcaccgatga ctgccatccc tgggttttgc cagtagtgaa gaaagtggag       300
     cagaagattg ctaatgacaa tagcctaaac cacgagtatc tgccaatcct gggcctggcc       360
     gagttccgga gctgtgcttc tcgtcttgcc cttggggatg acagcccagc tctcaaggag       420
     aagcgggtag gaggtgtgca gtctttgggg ggaacgggtg cacttcgaat tggggctgat       480
     ttcttagcgc gctggtacaa tggaacaaac aacaagaaca cacccgtcta tgtgtcctca       540
     ccaacctggg agaatcacaa tgctgtattt tccgctgctg gttttaaaga tattcggtcc       600
     tatcgctact gggatgcaga gaagagagga ctggacctcc agggcttcct gaatgatctg       660
     gagaacgctc ctgagttctc catcattgtc ctccatgcct gtgcacacaa cccaactggg       720
     accgacccaa ctccggagca gtggaagcag attgcttctg tcatgaagca ccggtttctg       780
     ttccccttct ttgactcagc ctatcagggc tttgcatctg gaaacctaga gagagatgcc       840
     tgggccattc gctattttgt gtctgaaggc ttcgagttct tctgtgccca gtccttctcc       900
     aagaacttcg ggctctacaa tgagagagtt gggaatctga ctgtggttgg aaaagaacct       960
     gagagcatcc tgcgagtcct ttcccagatg gagaagattg tgcggattac ttggtccaat      1020
     ccccccgccc agggagcacg aatcgtggcc gacaccctct ctaaccctga gctctttgag      1080
     gaatggaaag gtaatgtgaa gacaatggct gaccggattc tgaccatgag atctgaactc      1140
     agggcacgac tagaagccct caaaacccct gggacttgga accacatcac tgatcaaatt      1200
     ggcatgttca gctacactgg gttgaacccc aagcaggttg agtatctgat caatgaaaag      1260
     cacatctacc tgctgccaag tggtcgaatc aacgtgagtg gcttaaccac caaaaatcta      1320
     gattatgtgg ccacctccat ccatgaagcc atcaccaaaa tccagtgaag aaacaccacc      1380
     cgtccagtac caccaaagta gttctctgtc atgcgtgttc cctgcctgca caaacctaca      1440
     tgtacatatc atggattaga gacacttgca ggactgaaag gctgctctgg tgaggcggcc      1500
     tctgtttaaa ccgtccccac gtgaagagaa catcccttga gacgaatttg gagactggga      1560
     ttagagcctt gggaggtcaa agcaaattaa aatttttatt taagaataaa agagtacttt      1620
     gatcatgaga cataggtatc ttgtccccct ggctaaaagg caggagtatt gcgtgtggca      1680
     accacgtgct tctgtgtggt gtttgactct gtacaaagtc tagtcccaaa gatcaagttg      1740
     tctgaaggag ccaaagtgtg aatgtgggtg tcggctgtgg cattaaactc atcatctcaa      1800
     cccggagtgt ctggtctgcc tgctctttct gcatggtgtg tccctagtcc taagctttgg      1860
     ttctttaggg cgactgtgtt aagaaggata tttaatcatg acacacacat atttaactga      1920
     aacagaagtt tgaccgaaca gtattcactc gtgatgtgtg tagtgcgttc tatttttgag      1980
     ccattctact gtgttctact tcacctaaaa aagtaaaata aaaatgttga tcaagaaaaa      2040
     aaaaaaaaaa aaa                                                         2053
//
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