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EBI DbfetchID AB169509; SV 1; linear; mRNA; STD; MAM; 1876 BP. XX AC AB169509; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis brain cDNA, clone: QccE-12288, similar to human DE ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog,yeast) (UBE2D3), DE transcript variant 3, mRNA, RefSeq: NM_181887.1. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1876 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of:Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA, Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..1876 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QccE" FT /clone="QccE-12288" FT /db_xref="taxon:9541" FT CDS 200..643 FT /note="Homo sapiens ubiquitin-conjugating enzyme E2D 3 FT (UBC4/5 homolog,yeast) (UBE2D3), transcript variant 3, FT mRNA, RefSeq: NM_181887.1" FT /db_xref="GOA:Q4R5N4" FT /db_xref="InterPro:IPR000608" FT /db_xref="UniProtKB/Swiss-Prot:Q4R5N4" FT /protein_id="BAE01591.1" FT /translation="MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPY FT QGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLL FT SICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM" XX SQ Sequence 1876 BP; 561 A; 361 C; 373 G; 581 T; 0 other; agtcattgca gaggtaccgc ttcggttagc cagccacgaa gttctcgcga gagtcgtctt 60 cttaatacca agcgcctgtg tctggcagag ccgctgtgag acgagacaat cctgccccgc 120 cgccgggata atcaagagtt ttggccggac ctttgagcat acaccgagag agtgaggagc 180 cagacgacaa gcacacacta tggcgctgaa acggattaat aaggaactta gtgatttggc 240 ccgtgaccct ccagcacaat gttctgcagg tccagttggg gatgatatgt ttcattggca 300 agccacaatt atgggaccta atgacagccc atatcaaggc ggtgtattct ttttgacaat 360 tcattttcct acagactacc ccttcaaacc acctaaggtt gcatttacaa caagaattta 420 tcatccaaat attaacagta atggcagcat ttgtctcgat attctaagat cacagtggtc 480 gcctgcttta acaatttcta aagttctttt atccatttgt tcactgctat gtgatccaaa 540 cccagatgac cccctagtgc cagagattgc acggatctat aaaacagaca gagataagta 600 caacagaata tctcgggaat ggactcagaa gtatgccatg tgatgctacc ttaaagtcag 660 aataacctgc attatagctg gaataaactt taaattactg ttcctttttt gattttctta 720 tccggctgct cccctgtcag acctcatctt ttttaatttt attttttgtt tacctccctc 780 cattcattca catgctcatc tgagaagact taagttcttc cagctttgga caataactgc 840 ttttagaaac cgtaaagtag ttacaagaga acagttgccc aagactcaga attaaaaaaa 900 aaaaaatgga gcatgtgtat tatgtggcca atgtcttcac tctaacttgg ttatgagact 960 aaaaccattc ctcactgctc taacatgctg aagaaatcat ctgaggggga gggagatgga 1020 tgctcagttg tcacatcaaa gggtacagca ttattctagc agcatccatt cttgtttaag 1080 ccttccactg ttagagattt gaggttacat gatatacttt atgctcataa ctgatgtggc 1140 tggagaattg gtattgaatt tatagcatca gcagaacaga aaatgtgatg tattttatgc 1200 atgtcaataa aggaatgacc tgttcttgtt ctacagagaa tggaaattgg aagtcaaaca 1260 ccctttgtat tccaaaatag ggtctcaaac attttgtaat tttcatttaa attgttagga 1320 ggcttggagc tattagttaa tctatcttcc aatacactgt ttaatatagc actgaataaa 1380 tgatgcaagt tgtcaatgga tgagtgatca actaatagct ctgctagtaa ttgatttatt 1440 tttcttcaat aaagttgcat aaaccaatga gttagctgcc tggattaatc agtatgggaa 1500 acaatctttt gtaaatgcaa agctgttttt tgtatatact gttgggattt gcttcattgt 1560 ttgacatcaa atgatgatgt aaagttcgaa agagtggata ttttgccatg ttcagttaaa 1620 gtgcacagtc tgttacaggt tgacacattg cttgacctga tttatgcaga attaataagc 1680 tatttggata gtgtagcttt aatgtgctgc acatgatact ggcagcccta gagttcatag 1740 atggacttct gggacccagc agttttgaaa tgtgtttatg gagtttaaga aatttatttt 1800 ccaggtgcag cccctgtcta actgaaattt ctcttcacct tgtacacttg acagctgaaa 1860 aaaaaaaaaa aaaaaa 1876 // |
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