 |
|
EBI DbfetchID AB169404; SV 1; linear; mRNA; STD; MAM; 1888 BP. XX AC AB169404; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA, clone: QtsA-19729, similar to human DE ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C.elegans) (UBE2G1), DE transcript variant 1, mRNA, RefSeq: NM_003342.3. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1888 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of: Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA; Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..1888 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-19729" FT /db_xref="taxon:9541" FT CDS 190..702 FT /note="Homo sapiens ubiquitin-conjugating enzyme E2G 1 FT (UBC7 homolog, C.elegans) (UBE2G1), transcript variant 1, FT mRNA, RefSeq: NM_003342.3" FT /db_xref="GOA:Q4R5Y8" FT /db_xref="HSSP:2AWF" FT /db_xref="InterPro:IPR000608" FT /db_xref="InterPro:IPR016135" FT /db_xref="UniProtKB/Swiss-Prot:Q4R5Y8" FT /protein_id="BAE01487.1" FT /translation="MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGP FT PDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGY FT EKPEGRWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRK FT SQETAFE" XX SQ Sequence 1888 BP; 565 A; 416 C; 346 G; 561 T; 0 other; agtcgctgtg aaaagagctg aagcgagcgg actcgcaccg gcagcgaggc gccgctcccg 60 ccgcctcagc ccggcctccc tcggctccgg cgctccgggt tcctcggcac atcccgcccc 120 agccgccccc agagcctgtc cccagccctt cggaagcccc ggcgccagcc cgggccctcg 180 gcggggagga tgacggagct gcagtcggca ctgctactgc gaagacagct ggcagaactc 240 aacaaaaatc cagtggaagg cttttctgca ggtttaatag atgacaatga tctctaccga 300 tgggaagtcc ttattattgg ccctccagat acactttatg aaggtggtgt ttttaaggct 360 catcttactt tcccaaaaga ttatcccctc cgacctccta aaatgaaatt cattacagaa 420 atctggcacc caaatgttga taaaaatggt gatgtgtgca tttctattct tcatgagcct 480 ggggaagata agtatggtta tgaaaagcca gaaggacgct ggctccctat ccacacagtg 540 gaaaccatca tgattagtgt catttctatg ctggcagacc ctaatggaga ctcacctgct 600 aatgttgatg ctgcgaaaga atggagggaa gatagaaatg gagaatttaa aagaaaagtt 660 gcccgctgtg taagaaaaag ccaagagact gcttttgagt gacatttatt tagcagctag 720 taacttcact tatttcaggg tctccaattg agaaacatgg cactgttttt cctgcactct 780 acccacctat tgctggactt ctgttgtaca agttggcaaa cactggctgg aactgggctg 840 caataaaaca tgccagttat caatgctgac aagagcctaa caagtgccaa cttacagatg 900 attacgtatt tggaattcta atgaactgtt ttaaccttca ggaagaattg taaagacctg 960 tacatagcac aacatgatcc ggataatata tatactgttc atgtacatcc acaaatacac 1020 cttgtaccaa ataatgcttt cttgtagtag aataagaatc gtgtaaattc taaaagattt 1080 tagcaggttt tctttcctat tcattgtttc ttatcagttt aaaaggattc ctttaagcat 1140 gtcagatgaa aagcaattag gattaaaagt ttccatttaa tttcccttaa accattgagg 1200 cttcattaaa ctcttttcac ttactaaact tttgtatctt ctttgttttg acacactccc 1260 ctttgctttt gtctcttatc tgccagaatg ttctcaaatg atttagttcc aatactgaaa 1320 tacttaatga gcaattactt gatttttaat gatgacttcg aaggagtcat cactaggtgc 1380 tttgtccttt ttctattcta gttgcaccca cctcttggat tggatatagc aataacattt 1440 attggctgtt gtgagctctt gatcccagtc attacccccc gagaactaaa aatagatggt 1500 tcttaattca acttacagaa aatttccccc aaacaatagc aaatctgact tttccccctt 1560 cagttgcccg gtagtaaggt tggataaacg aagcatgcac agctacaggc tttctaccta 1620 acttctgggt ttgctattac aaatactatt tactctcata cccttctcct tagtccttca 1680 tatttctttg cctctattct tctatactgc agatttttct cacctattgt acaaagaaat 1740 tgcgatgtat attttcatgt aatttgattt tggaattctg tcaccttatg tagtgagttc 1800 ttccaaaata taattttttt ttcaataaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1860 aaaaaaaaaa aaaaaaaaaa aaaaaaaa 1888 // |
|