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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB169351; SV 1; linear; mRNA; STD; MAM; 1349 BP.
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AC   AB169351;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
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DE   Macaca fascicularis testis cDNA, clone: QtsA-19202, similar to human
DE   sorbitol dehydrogenase (SORD), mRNA, RefSeq: NM_003104.3.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-1349
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of: Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA; Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1349
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QtsA"
FT                   /clone="QtsA-19202"
FT                   /db_xref="taxon:9541"
FT   CDS             124..1197
FT                   /note="Homo sapiens sorbitol dehydrogenase (SORD), mRNA,
FT                   RefSeq: NM_003104.3"
FT                   /db_xref="GOA:Q4R639"
FT                   /db_xref="InterPro:IPR002328"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q4R639"
FT                   /protein_id="BAE01436.1"
FT                   /translation="MAAAAKPKNLSLVVHGPGDLRLENYPIPEPGPNEVLLRMHSVGIC
FT                   GSDVHYWEEGRIGNFIVKKPMVLGHEASGTVEKVGSLVKHLKPGDRVAIEPGVPRENDE
FT                   FCKSGRYNLSPSIFFCATPPDDGNLCRFYKHNAAFCYKLPDNVTFEEGALIEPLSVGIH
FT                   ACRRGGVTLGHRVLVCGAGPIGVVSLLVAKAMGAAQVVVTDLSAPRLSKAKEIGADLVL
FT                   QISKESPQEIAGKVEGLLGCKPEVTIECTGAEASIQAGIYATRSGGTLVLVGLGSEMTT
FT                   IPLLHAAVREVDIKGVFRYCNTWPVAISMLASKSVNIKPLVTHRFPLEKALEAFETFKK
FT                   GLGLKIMLKCDPNDQNP"
XX
SQ   Sequence 1349 BP; 322 A; 349 C; 374 G; 304 T; 0 other;
     gtgcgccgct tcttccaggc cccgccttcc accctgtgcc ctggaccctc ggctgggcag        60
     cgccaccaga gcgaccaaac gtcccgcgcc ttccaggccg cacccaagag ccaaaagagt       120
     tccatggcgg cggcggccaa gccgaagaac ctttccctgg tggtgcacgg accgggggac       180
     ttgcgcctgg agaactatcc catccctgaa ccaggcccaa atgaggtgtt gctgaggatg       240
     cattctgttg gaatctgcgg ctcagatgtc cactactggg aggagggtcg aattgggaat       300
     tttatcgtga aaaagcccat ggtgctggga catgaagctt caggaacggt cgaaaaagtg       360
     ggatcattgg taaagcacct aaaaccaggt gatcgtgttg ccatcgagcc tggtgttccc       420
     cgagaaaatg atgaattttg caagtctggc cgatacaatc tgtcaccttc catcttcttc       480
     tgtgccacac cccccgatga cggaaacctc tgccggttct acaagcacaa tgcagccttt       540
     tgttacaagc ttcctgacaa tgtcaccttt gaggaaggcg ccctgattga gccactttct       600
     gtggggatcc atgcctgcag gagaggtgga gtcaccctgg gacacagggt ccttgtgtgt       660
     ggagctgggc caatcggggt ggtctctttg ctcgtggcca aggcaatggg agcagctcaa       720
     gtagtggtga ctgacctgtc tgctccccgg ttgtccaaag ccaaggagat tggggctgat       780
     ttagtcctcc agatctccaa ggaaagtcct caggaaattg ccggtaaagt agaaggtctg       840
     ctggggtgca agccggaagt cactatcgag tgcacggggg cagaggcctc catccaggcg       900
     ggcatctacg ccactcgctc tggtgggacc ctggtgcttg tggggctggg ctctgagatg       960
     accaccatac ctctactgca tgcagccgtc cgggaggtgg atatcaaggg cgtgtttcga      1020
     tactgcaaca cgtggccagt ggcgatttcg atgcttgcat ccaagtctgt gaacataaaa      1080
     cccctcgtca cccatagatt tcctctggag aaagctctgg aagcctttga aacatttaaa      1140
     aagggattgg ggttgaaaat catgctcaag tgtgatccca atgaccagaa tccctgatgt      1200
     ttattggctt tgccctcatc cgcacagtat cgggatctca gggcacaatg gctggacagg      1260
     ggtgggcttt catgcagaac tttctctttt gaatgttaag aataattaat acaactcatc      1320
     atgaacagaa aaaaaaaaaa aaaaaaaaa                                        1349
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