 |
|
EBI DbfetchID AB169166; SV 1; linear; mRNA; STD; MAM; 1875 BP. XX AC AB169166; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA, clone: QtsA-17708, similar to human DE 2,3-bisphosphoglycerate mutase (BPGM), transcriptvariant 1, mRNA, RefSeq: DE NM_001724.3. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1875 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of: Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA; Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..1875 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-17708" FT /db_xref="taxon:9541" FT CDS 271..1050 FT /note="Homo sapiens 2,3-bisphosphoglycerate mutase (BPGM), FT transcriptvariant 1, mRNA, RefSeq: NM_001724.3" FT /db_xref="GOA:Q4R6L7" FT /db_xref="HSSP:1T8P" FT /db_xref="InterPro:IPR001345" FT /db_xref="InterPro:IPR005952" FT /db_xref="InterPro:IPR013078" FT /db_xref="UniProtKB/Swiss-Prot:Q4R6L7" FT /protein_id="BAE01258.1" FT /translation="MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQL FT KALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMA FT LNHGEEQVRLWRRSYNITPPPIEESHPYYHEIYNDRRYKVCDVPLDQLPRSESLKDVLE FT RLLPYWNERIAPEVLRGKTVLISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLEL FT DENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK" XX SQ Sequence 1875 BP; 587 A; 358 C; 432 G; 498 T; 0 other; gtcgatgccg gcggcagtga tgagtcctag gaggcgctgg ctcttaggct gttcggagga 60 gcggctgctg ctgctgctgc tactagtagc ccctttgcag acagaaaaac tcgaatgcgg 120 tgaaaagaga aggaggtgaa tatatgcagt gttttcatgt aggaatctgg agccaatcac 180 aagttcttag aaccactaga aataaaaaca tgtattgctg tccttgaata ttagcccatt 240 tgaaaacgcc tgggaagttc agccatcagt atgtccaagt acaaacttat tatgttaaga 300 catggtgagg gtgcttggaa taaggagaac cgtttttgta gctgggtgga tcagaaactc 360 aacagtgaag gcatggagga agctcggaac tgtgggaagc aactcaaagc attaaacttt 420 gagtttgatc ttgtattcac atctgtcctt aatcggtcca ttcacacagc ctggctgatc 480 ctagaagagc tagggcagga atgggtgccc gtggaaagct cctggcgtct aaatgagcgt 540 cactatgggg ccttgatcgg tctcaacagg gagcaaatgg ctttgaatca tggtgaagaa 600 caagtgaggc tctggagaag aagctacaat ataaccccgc ctcccattga ggagtctcat 660 ccttactacc acgaaatcta caacgaccgg aggtataaag tatgtgatgt gcccttggat 720 cagctgccac ggtctgaaag cttaaaggat gttctggaaa gactccttcc ctattggaat 780 gaaaggatag ctcctgaagt cttacgtggc aaaaccgttc tgatatctgc tcatggaaat 840 agcagtaggg cactcctaaa acacctggaa ggtatctcag atgaagacat catcaacatt 900 actcttccta ctggagtccc cattcttctg gaactggatg aaaacctgcg tgctgttggg 960 cctcatcagt tcctgggtga ccaagaggcg atccaagcag ccattaagaa agtagaagat 1020 caaggaaaag tgaaacaagc taaaaaatag tctctcccaa ctgttggcta agaagaaatg 1080 caaaagaagt ggcataggag tgtgttatgg gtgctgaact ctctctcttt ttccccgatt 1140 ttccagagct aggctgtgga gtagagtttg tataggtaac taggtaactt attgtgaccc 1200 agataaggct ttaggatgcc tcagtgctta tgtcacagcc ttatgagtta gctttcctgc 1260 tagcccccta gtcagtcact aaactagtaa ctagtggggt ttaatgaagg tcatcagttt 1320 ctgagatggg agagcaacaa gtagagatga agttaaaggt atttatcatc caggaaatca 1380 tttctgagtc accattgaca ggcactattc taatcagtag ttcactttta tatttagtaa 1440 gattttctgg ggtgacaata agagatatta gataatatac catatgtatt tattactagt 1500 cttttcctct aggaaaaggg atgctttgat aattaaggcc agaggcccat taaatgagaa 1560 agtcacagat atatttctcc aagaaagcca acaaccgcca ccacaatgac agaaatgaca 1620 acaaggccct ttaacttgtc ttctagttta gcgacatcct tcatttgaca tttagtagaa 1680 ttcctctttg gccacaagaa taagcagcag atagacaact atggctgttg aggttctcat 1740 tttggtttgt tttaattttt cgaacttcaa tgtttgggta cctgtaatta gtttaaaaat 1800 aaagttcctg acaataaagt gactgaaaat ggcaaaaaaa aaaaaaaaaa aaaaaaaaaa 1860 aaaaaaaaaa aaaaa 1875 // |
|