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EBI DbfetchID AB169152; SV 1; linear; mRNA; STD; MAM; 2019 BP. XX AC AB169152; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA, clone: QtsA-17567, similar to human DE transmembrane phosphoinositide 3-phosphatase and tensinhomolog 2 (TPTE2), DE transcript variant 3, mRNA, RefSeq: NM_199254.1. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-2019 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of: Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA; Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..2019 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-17567" FT /db_xref="taxon:9541" FT CDS 107..1807 FT /note="Homo sapiens transmembrane phosphoinositide FT 3-phosphatase and tensinhomolog 2 (TPTE2), transcript FT variant 3, mRNA, RefSeq: NM_199254.1" FT /db_xref="GOA:Q4R6N0" FT /db_xref="InterPro:IPR000340" FT /db_xref="InterPro:IPR008973" FT /db_xref="InterPro:IPR014019" FT /db_xref="InterPro:IPR014020" FT /db_xref="InterPro:IPR016130" FT /db_xref="UniProtKB/Swiss-Prot:Q4R6N0" FT /protein_id="BAE01245.1" FT /translation="MCRVMKASFSRKVKLELCLLSDCALLSSSSAPTNELSGTNLEAHI FT NESPDPNALVGVIIERSPSDSTQTNEFKGATKETLTIETPHSSECKGAGLLSPVSKSML FT ERLSKFEVEDAENVASYDTKIKKIVRSIVSSFAFGIFGVFLVLLDVTLLLADLIFNDSK FT LYIPLVYRSISLAIALFFLMDVLLRVFVEGRQHYFSDLLNVLDTAIIVTPLLVDVVYIF FT FDIKFLRNIPRWIHLVRLLRLIILIRIFHLIHQKRELEKLMRRLVSENKRRYTRDGFDL FT DLTYVTERIIAMSFPSSGRQSFYRNPIEEVVRFLDKKHPNHYRVYNLCSERAYDPKYFH FT NRVSRIMIDDHNVPTLHEMVVFTKEVNEWMAQDPANIVAIHCKGGKGRTGTMICAFLIA FT SEIFLTAEESLYYFGERRTDKTNSSKFQGVETPSQNRYVGYFAQVKHLYNWNLPPRRIL FT FIKRFIIYSIRGVGTGGVCDLKVRIVMEKKVVFSSTSLGNCSILHDIETDRVLIDVFSG FT PPLYDDVKVQFFSSNLPKYYDNCPFFFWFNTSFIQSNRHILHSFRLVFT" XX SQ Sequence 2019 BP; 634 A; 367 C; 382 G; 636 T; 0 other; agtctaagat gtcattgcca tcatataaat cctcctttta ataaaaaaat gacatttcag 60 aaccaaatag cgctatacac gtgaatagta agaacttaac tggttcatgt gcagagtaat 120 gaaagcgagt ttttccagaa aagtgaaatt ggaattgtgt ctcctgtctg actgtgctct 180 tttatcatcc tctagtgcac ccacaaatga attatcaggc acgaacctgg aggcgcatat 240 caatgaaagt cccgatccga acgccctggt gggagtcatc attgaaagaa gccccagtga 300 cagtacacag acaaatgaat ttaaaggagc aaccaaggag acacttacga ttgaaacccc 360 acacagcagt gaatgtaaag gggcaggcct gttgtcacct gtcagtaaaa gtatgttaga 420 acgactttcc aagtttgaag ttgaagatgc tgaaaatgtt gcttcatatg acaccaagat 480 taagaaaatt gtgcgttcaa ttgtatcatc ctttgcattt ggaatatttg gagttttcct 540 ggtcttactg gatgtcactc tcctccttgc cgacctaatt ttcaatgata gcaaacttta 600 tattcctttg gtgtatcgtt ctatttctct agctattgcc ttattttttc tcatggatgt 660 tcttcttcga gtatttgttg aagggagaca gcactatttt tctgacttac ttaacgtttt 720 agataccgcc attattgtga ctcctctgct ggttgatgtc gtttacattt tttttgacat 780 taagtttctt aggaatattc ccagatggat acatttagtt cgacttctac gacttattat 840 tctgataaga atttttcatc tgattcatca aaaaagagaa cttgaaaagc tgatgagaag 900 gctggtttca gaaaacaaaa ggcgatacac aagggatgga tttgacctag acctcactta 960 tgttacagaa cgtattatcg ctatgtcatt tccatcttct ggaaggcagt ctttctatag 1020 aaatccaatt gaggaagtcg tgcggtttct agataagaaa catccaaatc actatcgagt 1080 ctataatcta tgcagtgaaa gagcttatga tcctaagtac ttccataata gggtcagtag 1140 aatcatgatt gatgatcata atgtccccac tctacatgag atggtggttt tcaccaagga 1200 agtaaatgag tggatggctc aagatcctgc aaacatcgta gcaattcact gtaaaggagg 1260 caaaggaaga actggaacta tgatttgtgc cttccttatt gcctccgaaa tatttttaac 1320 tgcagaggaa agcctatatt attttggaga aaggcgaaca gataaaacca acagcagtaa 1380 atttcaggga gtagaaactc cttctcagaa tagatatgtt ggatattttg cacaagtgaa 1440 acatctctac aactggaatc tccccccgag acggatactc tttataaaaa gattcattat 1500 ttattcgatt cgtggtgttg gaacaggcgg tgtatgtgat ctaaaagtcc gaatagtaat 1560 ggagaaaaaa gttgtctttt ccagtacttc attaggaaat tgttcgatat tgcatgacat 1620 tgaaacagac agagtgttaa ttgatgtatt cagcggtccc cctctgtatg acgacgtgaa 1680 ggtacagttt ttctcttcga atcttcctaa atactatgac aattgtccgt ttttcttctg 1740 gttcaacaca tcttttattc aaagtaacag gcacatactt cacagtttcc gtcttgtatt 1800 tacataaatt tggaacacag tatgcaggaa cagtcagcat cattttgaag aactttgaaa 1860 tagaactttc atatcaaaga cttgagatat ttaaaaattg tgattgccta gcacccactt 1920 acatattttt ggtcaagtat ttattccctg ctgtggttca catttgtaat ttcagattga 1980 ttagaaagct aatttataaa aaaaaaaaaa aaaaaaaaa 2019 // |
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