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EBI DbfetchID AB169063; SV 1; linear; mRNA; STD; MAM; 1723 BP. XX AC AB169063; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA, clone: QtsA-16987, similar to human DE pyrroline-5-carboxylate reductase family, member 2(PYCR2), mRNA, RefSeq: DE NM_013328.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-1723 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of: Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA; Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..1723 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-16987" FT /db_xref="taxon:9541" FT CDS 158..1120 FT /note="Homo sapiens pyrroline-5-carboxylate reductase FT family, member 2(PYCR2), mRNA, RefSeq: NM_013328.2" FT /db_xref="GOA:Q4R6W7" FT /db_xref="InterPro:IPR000304" FT /db_xref="InterPro:IPR004455" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q4R6W7" FT /protein_id="BAE01157.1" FT /translation="MSVGIIGAGQLAYALARGFTAAGIVSAHKIIASSPEMNLPTVSAL FT RKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSV FT EKKLMAFQPAPKVIRCMTNTPVVVREGATVYAMGTHALVEDGQLLEQLMSSVGFCTEVE FT EDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQ FT HPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAA FT LKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD" XX SQ Sequence 1723 BP; 423 A; 461 C; 480 G; 359 T; 0 other; gagtccagtg ttgcaccatc ccagaaactg ctgttagccc gtcctcgctc ggctcgccgc 60 gcgttggccc ctgcgctgtc cgcccttccc ctagcgttat ttccgggccc gcgctgagga 120 ggttcctgcg gctgccagga ggtgtgagcc gcggaccatg agcgtgggca tcatcggagc 180 cggccagctg gcctatgctc tggcgcgggg cttcacggcc gcaggtatcg tgtcggctca 240 caagataata gccagctctc cggaaatgaa cctgcccacg gtgtcggccc tcaggaagat 300 gggtgtgaac ctgacacgca gcaacaagga gacagtgaag cacagcgacg tcctgtttct 360 ggccgtgaaa ccacatatca tccccttcat tctggacgag attggggccg atgtacaagc 420 cagacacatc gtggtctcct gtgcggctgg tgtcaccatc agctctgtgg agaagaagct 480 gatggcgttc cagccagccc ccaaagtgat tcgctgcatg accaacacac ctgtggtagt 540 gcgggaaggc gctacagtgt acgccatggg cacccatgct ctggtggagg atgggcagct 600 cctggagcaa ctcatgagca gcgtgggctt ctgcactgaa gtggaagagg acctcatcga 660 tgccgtcacg gggctcagtg gcagcgggcc tgcctatgca ttcatggctc tggatgcatt 720 ggctgatggt ggggtgaaga tgggtttgcc acggcgcctg gcagtccgac tcggggccca 780 ggccttgctg ggagctgcca agatgctgct ggactcagag cagcatccat gccagctcaa 840 ggacaatgtc tgctcccctg ggggagccac catccacgcc ctgcactttc tggagagtgg 900 gggcttccgc tctctgctca tcaatgcagt tgaggcctcc tgtatccgaa cacgagagct 960 acagtccatg gccgaccaag aaaagatctc cccagctgcc cttaagaaga cgctcctaga 1020 cagagtgaag ctggaatccc ccacagtctc cacactgacc ccctccagcc cagggaagct 1080 ccttacaaga agcctggccc tgggtggcaa gaaggactaa ggcagcgtct accccctctg 1140 tgatttagag ccctcagttg agagcccctg ccccaccccc tccattgctc ctcctcacct 1200 ttgcaaggag aaagcaggct caggtagttt tcagggcttt gtgataaaac ctccttaaat 1260 ctgttcagac caagcagtgt gagcttcctc tcctgtccca tgttggaaga tgctctgaag 1320 gggtggtaga tgctggaagc cagacacaac cctgcatacc ccactcaatt ggcggcagct 1380 ggggctggga ctggagtcag cccagctggg aggaagggct ggggaggatc tgcagctgaa 1440 gcccaaggca gggctagttt gatgccaagg caaagtggtg agagagaaaa taggaaaggg 1500 gcttttctct gaattggtaa atgggaaaga agtgagcaac ttaagattgt caccatcaca 1560 agtgtacagg attagactgt gtttatattt aactcttgct tcataggtgt accatttaat 1620 gctaagttta actgctttaa taaagtttaa taaagtttat ttttaaataa aaaaaaaaaa 1680 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 1723 // |
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