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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB168751; SV 1; linear; mRNA; STD; MAM; 1840 BP.
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AC   AB168751;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
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DE   Macaca fascicularis testis cDNA, clone: QtsA-14570, similar to human
DE   ATP/GTP-binding protein (HEAB), mRNA, RefSeq: NM_006831.1.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-1840
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of: Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA; Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1840
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QtsA"
FT                   /clone="QtsA-14570"
FT                   /db_xref="taxon:9541"
FT   CDS             89..1366
FT                   /note="Homo sapiens ATP/GTP-binding protein (HEAB), mRNA,
FT                   RefSeq: NM_006831.1"
FT                   /db_xref="GOA:Q4R7R3"
FT                   /db_xref="InterPro:IPR010655"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q4R7R3"
FT                   /protein_id="BAE00859.1"
FT                   /translation="MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIF
FT                   GTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMR
FT                   RQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTM
FT                   GALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRAS
FT                   VSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPK
FT                   SGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLP
FT                   LGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVF
FT                   TVLSPAPRPLPKNFLLIMDIRFMDLK"
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SQ   Sequence 1840 BP; 515 A; 405 C; 465 G; 455 T; 0 other;
     aaagttagta aaaagaggat tatagacgtg gtggagcctg ttggctgagg gacctagacc        60
     tagacagcgc agcagctaca cagaagagat gggagaagag gctaatgatg acaagaagcc       120
     aaccactaaa tttgaactag agcgagaaac agaacttcgc tttgaggtgg aggcatctca       180
     gtcagttcag ttggagttgt tgactggcat ggcagagatc tttggcacag agctgacccg       240
     aaacaagaaa ttcacctttg atgctggtgc caaggtggct gttttcactt ggcatggctg       300
     ttctgtgcaa ctgagcggcc gcactgaggt ggcttatgtc tccaaggaca ctcctatgtt       360
     gctttacctc aacactcaca cagccttgga acagatgcgg aggcaagcgg aaaaggaaga       420
     agagcgaggt ccccgtgtga tggtagtggg ccccactgat gtgggcaagt ctacagtgtg       480
     tcgccttctg ctcaactatg cagtgcgttt gggccgccgt cccacttatg tggagctgga       540
     tgtgggccag ggttctgtgt ccatccctgg taccatgggg gccctctaca ttgagcgacc       600
     tgcagacgtg gaagagggtt tctctatcca ggcccctctg gtgtatcatt ttggttccac       660
     cactcctggc accaacatca agctttataa taagattaca tctcgtttag cagatgtgtt       720
     caaccaaagg tgtgaggtga accgaagggc atctgtgagt ggctgtgtca ttaacacctg       780
     tggctgggtc aagggctctg gttaccaggc tctggtgcat gcagcctcag cttttgaggt       840
     ggatgtcgtt gtggttctgg atcaagaacg actgtacaac gaactgaaac gggacctccc       900
     ccactttgta cgcaccgtgc tgctccctaa atctgggggt gtggtggagc gctccaagga       960
     cttccggcgg gaatgtaggg atgagcgtat ccgtgagtat ttttatggat tccgaggctg      1020
     tttctatccc catgccttca atgtcaaatt ttcagatgtg aaaatctaca aagttggggc      1080
     acccaccatc ccagactcct gtttaccttt gggcatgtct caagaggata atcagctcaa      1140
     gctagtaccc gtcactcctg ggcgagatat ggtgcaccac ctactgagtg ttagcactgc      1200
     cgagggcaca gaggagaacc tgtctgagac gagcgtagct ggcttcattg tggtgaccag      1260
     tgtggacctg gagcatcagg tgtttactgt tctgtctcca gccccccgcc cactgcctaa      1320
     gaacttcctt ctcatcatgg acatccggtt catggatctg aagtagagat cagcaggaag      1380
     ccttgctgcc tgggacatag agatcttctg gccaccccta gaggcagatg ggctgagata      1440
     agagactctg ttggggccac ctaaccagta aactgtggac tagtagaagt tcatattcta      1500
     cctctaaaaa caggtagtgg taacctgact cttctaatct ggaaccaaaa ggaaaaccat      1560
     gagactctaa ttggttgctt agactaccta agatgccact ttgaattctc taagaccctg      1620
     gagaattgca tttctttcac tgtgctactt tgtggttttt aaaaatcaat gctttatatt      1680
     ccatataggg ttcttaccca tttatctagg atgaaagtgt gaattagagg gactctttcc      1740
     aataaagttc aaacttaaaa ataaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      1800
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                            1840
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