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EBI DbfetchID AB168645; SV 1; linear; mRNA; STD; MAM; 2390 BP. XX AC AB168645; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA clone: QtsA-13749, similar to human lactate DE dehydrogenase A -like (LDHL), mRNA, RefSeq: NM_033195.1. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-2390 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of: Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA; Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..2390 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-13749" FT /db_xref="taxon:9541" FT CDS 73..1218 FT /note="Homo sapiens lactate dehydrogenase A -like (LDHL), FT mRNA, RefSeq: NM_033195.1" FT /db_xref="GOA:Q4R816" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q4R816" FT /protein_id="BAE00756.1" FT /translation="MSWTVPLVRASQRVSSVGANFLCLKMALCPRQAARIPLKGAWRFT FT SVSKMATVKSELIERFTSEEPVHHSKVSIIGTGSVGMACAISILLKGLIDELALVDLDE FT GKLKGETMDLQHGSSFTKMPNIVCSKDYFVTANSNLVIITAGARQEKGETRLNLVQRNV FT ALFKLMISNIVQHSPHCKLIIVSNPVDILSYVAWKLSAFPKNRVIGSGCNLDTARFRFL FT IGQKLGIHSESCHGWILGEHGDSSVPVWSGVNIAGVPLKDLNSDIGTDKDPEQWKNVHE FT EVIATAYEIIKMKGYTSWAIGLSVADLTESILKNLRRTHPVSTIIKGLYGIDEEVFLSI FT PCILGENGITHLIKIKLTPEEEDRLKKSAKTLWEIQKELKI" XX SQ Sequence 2390 BP; 734 A; 485 C; 494 G; 677 T; 0 other; ctcttggtgt ctcaacgttc tggtcagcag ctttttgccg tttctctctc tccacttctt 60 gagcgagcag ccatgagttg gactgtgcct cttgtgcggg ccagccagag agtgagctcg 120 gtgggagcga atttcctgtg cctgaagatg gccctgtgtc cccgtcaggc agcgcgcatc 180 ccactcaagg gcgcctggcg cttcacctcc gtgagcaaga tggcgactgt gaagagtgag 240 cttattgagc gcttcacttc tgaggagccc gttcatcaca gtaaggtctc catcatagga 300 actggatcgg tgggcatggc ctgcgctatc agcatcttat taaaaggctt gatcgatgaa 360 cttgcccttg tggatcttga tgaaggcaaa ctgaagggtg agacgatgga tcttcaacat 420 ggcagctctt tcacgaaaat gccaaatatt gtttgtagca aagattactt tgtcaccgca 480 aactccaacc tagtgattat cacagcaggt gcacgccaag aaaagggaga aacgcgcctt 540 aatttagtgc agcgaaatgt ggccctcttc aagttaatga tttccaatat tgtccagcat 600 agtccccact gcaaactgat tattgtttcc aatccagtgg atatcttaag ttatgtagct 660 tggaagctga gtgcatttcc caaaaaccgt gttattggaa gcggctgtaa cctggatacc 720 gctcgttttc gtttcttgat tggacaaaag cttggtatcc attctgaaag ctgccatgga 780 tggatcctcg gagagcacgg agactcaagt gttcctgtgt ggagtggagt gaacatagct 840 ggtgtccctt tgaaggacct gaactctgat ataggaactg ataaagatcc tgagcaatgg 900 aaaaatgtcc acgaagaagt gattgctact gcctatgaga ttattaaaat gaaaggttat 960 acttcttggg ccattggcct atctgtggct gatttaacag aaagtatttt gaagaatctt 1020 aggagaacac atccagtttc caccataatt aagggcctct atggaataga tgaagaagta 1080 ttcctcagta ttccttgtat cctgggagag aatggtatta cccatcttat aaagataaag 1140 ctgacccctg aagaagagga ccgtctgaaa aaaagtgcaa aaacactttg ggaaattcag 1200 aaggagctca agatttaaag ttgtctaaaa ctaccattcc gaaattattg aagagatcac 1260 agatacagga ttatataacg aaattttgaa taaacttgaa ttcctaaaag atggaaacag 1320 gaaagtaggt agagtgactt tcctatttat ttagtcctcc agctctttta tttagcatcc 1380 acgtgctgga tgatacttat ttacaattcc taagtgtttt tggtaactct gatgtaacac 1440 aattcctaag tgtttttggt accacttgcc ttgtatatac atgttgttag catttagttc 1500 ccaaaaagta ggatgtaggt atttattgtg ttctagaaat tctgagtctt ttcattagat 1560 atatgctatt tctttcattc ttgctgtctt acacctatgt tcatttatac gctgtaaagt 1620 agtagcttct tctacagtat aaaaataaaa gtatgtacat acaaaaaata tgcagtagta 1680 tatacaatct tttgttttgc ttcttttgat agtcaataag ttctatttgt tgaatgaata 1740 aaatacggcg aggcatatga tttgcttcat gagaaaccgg atcacagacc caaaagctta 1800 aaacagatag acattaaaat ggatattggc cataccttcc cagcataatg atgaatgatg 1860 aagccttggt tccaactgtt gaagtgctca tgactcccaa tctgcatctg aagtttctgg 1920 agcagtgtct gatctgcccc ctcacccacc gcatgcatcg tggcgcacac atcatccagg 1980 atgctcatga taccaggagg gttctgatgg gagcaagaag gcaaggccct agtcactgac 2040 ctctccaaaa cagacaatgc ttatcaggtc ctttcttagg gaaggaaaca gctccccaga 2100 cttaaaatac catcttgaaa gtcagtcttc cccttcaggc aagaatgcaa ttaatagtcc 2160 caaatgggaa gtcaccactt agatctaaag agggaggaaa ctccattttc caaaaagctg 2220 tttaactccc tgaagtttta tccaacagtc atgcacagat cattatctgc tcactcatat 2280 tcatgccaaa ttgacttaag aatccaaaag taaaataaaa acattttcct ttgacctaaa 2340 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2390 // |
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