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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB168595; SV 1; linear; mRNA; STD; MAM; 2066 BP.
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AC   AB168595;
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DT   20-JUN-2005 (Rel. 84, Created)
DT   07-OCT-2008 (Rel. 97, Last updated, Version 4)
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DE   Macaca fascicularis testis cDNA clone: QtsA-13311, similar to human
DE   tyrosylprotein sulfotransferase 2 (TPST2), mRNA, RefSeq: NM_003595.2.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-2066
RA   Hashimoto K., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RG   International consortium for macaque cDNA sequencing and analysis
RA   ;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications";
RL   Unpublished.
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RN   [3]
RX   DOI; 10.1093/molbev/msi187
RX   PUBMED; 15944441.
RA   Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S.,
RA   Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.;
RT   "Substitution rate and structural divergence of 5'UTR evolution:
RT   comparative analysis between human and cynomolgus monkey cDNAs";
RL   Mol. Biol. Evol. 22(10):1976-1982(2005).
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CC   The International consortium for macaque cDNA sequencing
CC   and analysis consists of: Department of Virology and Human
CC   Genome Center, Institute of Medical Science, The University of
CC   Tokyo, Tokyo, Japan; Division of Genetic Resources, National
CC   Institute of Infectious Diseases of Japan, Tokyo, Japan; National
CC   Health Research Institute, Taipei, Taiwan; Institute of Molecular
CC   Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
CC   Evolution, University of Chicago, Chicago, IL, USA; Center for
CC   Information Biology, National Institute of Genetics of Japan,
CC   Mishima, Japan.
CC   Clone distribution: clone distribution information can be
CC   found at: http://www.nih.go.jp/yoken/genebank/
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method. Libraries were made from:
CC                QccE: cerebellum cortex
CC                QnpA: parietal lobe
CC                QtrA: temporal lobe right
CC                QflA: frontal lobe left
CC                QmoA: medulla oblongata
CC                QbsA: brain stem
CC                QorA: occipital lobe right
CC                QtsA: testis
CC   Custom primers were used for 5' and 3'-end sequencing. The
CC   full-insert sequencing was done by primer-walking method using ABI
CC   DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..2066
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque cDNA library QtsA"
FT                   /clone="QtsA-13311"
FT                   /db_xref="taxon:9541"
FT   CDS             424..1557
FT                   /note="Homo sapiens tyrosylprotein sulfotransferase 2
FT                   (TPST2), mRNA, RefSeq: NM_003595.2"
FT                   /db_xref="GOA:Q4R863"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q4R863"
FT                   /protein_id="BAE00709.1"
FT                   /translation="MRLSVRRVLLAAGCALVLVLAVQLGQQVLECRAVLAGLRSPRGAM
FT                   RPEQEELVMVGTNHVEYRYGKAMPLIFVGGVPRSGTTLMRAMLDAHPEVRCGEETRIIP
FT                   RVLAMRQAWSKSGREKLRLDEAGVTDEVLDAAMQAFILEVIAKHGEPARVLCNKDPFTL
FT                   KSSVYLSRLFPNSKFLLMVRDGRASVHSMITRKVTIAGFDLSSYRDCLTKWNKAIEVMY
FT                   AQCMEVGKDKCLPVYYEQLVLHPRRSLKLILDFLGIAWSDAVLHHEDLIGKPGGVSLSK
FT                   IERSTDQVIKPVNLEALSKWTGHIPGDVVRDMAQIAPMLAQLGYDPYANPPNYGNPDPI
FT                   VVNNTQRVLKGDYKTPANLKGYFQVNQNSTSSHLGSS"
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SQ   Sequence 2066 BP; 484 A; 581 C; 552 G; 449 T; 0 other;
     ataaattcga ctgcagtgga gtccagcttc acaatcatat gagccccctg aaattaactt        60
     ccatagttct tcaccttcaa ggtcctattg ttgggctctg ccagcagaca gccttggcac       120
     acaggcacaa gagctggagc ccagagatga gagtgcccac aggagacgct gaatcgaggg       180
     tacacatgaa gccccaccct cttcagcaga acttcatcag cacgtttttc tgaagagaag       240
     gaaattaacc aaggcacaga gaggccggtg accccagcct gagatccatc tctgctttgt       300
     cagctgcttc tcacatttcc acagcagcct ctgagatgtg agcctggcgg gctgccagct       360
     aacctgtcgc tgaagcccca gaagcgggtc ctcaggccgg gcctgccccg cctccggccc       420
     agcatgcgcc tgtcggtgcg gagggtgctg ctggcagctg gctgtgccct ggtcctagtg       480
     ctggcggttc agctggggca gcaggtgcta gagtgccggg cggtgctggc gggcctgcgg       540
     agcccccggg gggccatgcg gccggagcag gaggagctgg tgatggtggg caccaaccac       600
     gtggaatacc gctacggcaa ggccatgccg ctcatcttcg tgggcggcgt gccacgcagt       660
     ggcaccacgt tgatgcgcgc catgctggac gcccaccccg aggtgcgctg cggcgaggag       720
     acccgcatca tcccgcgcgt gctggccatg cgccaggcct ggtccaagtc tggccgtgag       780
     aagctgcggc tggacgaggc gggggtgacg gatgaggtgc tggacgccgc catgcaggcc       840
     ttcatcctgg aggtgattgc caagcacgga gagccggccc gcgtgctctg caacaaggac       900
     ccatttacgc tcaagtcctc ggtctacttg tcgcgactgt tccccaactc caaattcctg       960
     ctgatggtgc gggatggccg agcctccgtg cactccatga tcacgcgcaa agtcaccatc      1020
     gcgggcttcg acctcagcag ctaccgcgac tgcctcacca agtggaacaa ggccatcgag      1080
     gtgatgtacg cccagtgcat ggaggtgggc aaggacaagt gcctgcctgt gtactacgag      1140
     cagctggtgc tgcaccccag gcgctcactc aagcttatcc tcgacttcct tggcatcgcc      1200
     tggagcgacg ctgtcctcca ccatgaagac ctcattggca agccgggtgg tgtctccctg      1260
     tccaagattg agcggtccac ggaccaggtc atcaagcctg ttaacctgga agcactctcc      1320
     aagtggactg gccacatccc tggggatgtg gtgcgggaca tggcccagat cgcccccatg      1380
     ctggctcagc tcggctatga cccttatgca aaccccccca actacggcaa ccctgacccc      1440
     attgtcgtca ataacacaca gcgggtcttg aaaggggact ataaaacacc agccaatctg      1500
     aaaggatatt ttcaggtgaa ccagaacagc acctcctccc acttaggaag ctcgtgattt      1560
     ccagatctct gcaaatgaac ttcattgcca agaagagaag aaactgcatt taagtggaaa      1620
     tcggacctct aatccaagca tattgcttgc tattaatcgc caaaacagga ctgctgatga      1680
     ggaatgtatt tgcatatgtt tgcaaaagct gaatcattga aaatgtacct tgaaactctc      1740
     tatctttgga cacgccaggg tagagaacga agggtatgga agtagtccgg cttttgaaac      1800
     ttaggtattt tatatttttc ccctcaagaa ctttttttta agagaaagat ttgccatcct      1860
     ccttaatttg caggactgcc ttggtggctt tgtttgctgg gacaaggccc acaaactgtg      1920
     cctctcctat tgacccttac tttgaattca aagaatctat ttaagaattt aatatatgag      1980
     gctttctttg attcctcctc agttctacct agtttcacag agaaaaaaaa atactctttg      2040
     aataaagtga aaaaaaaaaa aaaaaa                                           2066
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