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EBI DbfetchID AB168541; SV 1; linear; mRNA; STD; MAM; 3710 BP. XX AC AB168541; XX DT 20-JUN-2005 (Rel. 84, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis testis cDNA clone: QtsA-12874, similar to human DE 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4(PFKFB4), mRNA, DE RefSeq: NM_004567.2. XX KW fis (full insert sequence); FLI_CDNA; oligo capping. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-3710 RA Hashimoto K., Kusuda J., Sugano S.; RT ; RL Submitted (18-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RG International consortium for macaque cDNA sequencing and analysis RA ; RT "DNA sequences of macaque genes expressed in brain or testis and its RT evolutionary implications"; RL Unpublished. XX RN [3] RX DOI; 10.1093/molbev/msi187 RX PUBMED; 15944441. RA Osada N., Hirata M., Tanuma R., Kusuda J., Hida M., Suzuki Y., Sugano S., RA Gojobori T., Shen C.-K.J., Wu C.I., Hashimoto K.; RT "Substitution rate and structural divergence of 5'UTR evolution: RT comparative analysis between human and cynomolgus monkey cDNAs"; RL Mol. Biol. Evol. 22(10):1976-1982(2005). XX CC The International consortium for macaque cDNA sequencing CC and analysis consists of: Department of Virology and Human CC Genome Center, Institute of Medical Science, The University of CC Tokyo, Tokyo, Japan; Division of Genetic Resources, National CC Institute of Infectious Diseases of Japan, Tokyo, Japan; National CC Health Research Institute, Taipei, Taiwan; Institute of Molecular CC Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & CC Evolution, University of Chicago, Chicago, IL, USA; Center for CC Information Biology, National Institute of Genetics of Japan, CC Mishima, Japan. CC Clone distribution: clone distribution information can be CC found at: http://www.nih.go.jp/yoken/genebank/ CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC oligo-capping method. Libraries were made from: CC QccE: cerebellum cortex CC QnpA: parietal lobe CC QtrA: temporal lobe right CC QflA: frontal lobe left CC QmoA: medulla oblongata CC QbsA: brain stem CC QorA: occipital lobe right CC QtsA: testis CC Custom primers were used for 5' and 3'-end sequencing. The CC full-insert sequencing was done by primer-walking method using ABI CC DNA sequencer. XX FH Key Location/Qualifiers FH FT source 1..3710 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque cDNA library QtsA" FT /clone="QtsA-12874" FT /db_xref="taxon:9541" FT CDS 192..1601 FT /note="Homo sapiens FT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase FT 4(PFKFB4), mRNA, RefSeq: NM_004567.2" FT /db_xref="GOA:Q4R8B6" FT /db_xref="HSSP:1BIF" FT /db_xref="InterPro:IPR001345" FT /db_xref="InterPro:IPR003094" FT /db_xref="InterPro:IPR013078" FT /db_xref="InterPro:IPR013079" FT /db_xref="InterPro:IPR016260" FT /db_xref="UniProtKB/Swiss-Prot:Q4R8B6" FT /protein_id="BAE00656.1" FT /translation="MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVMV FT GLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRNMVKTYKSFEFFLPDNEEGLKIRK FT QCALAALRDVRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICVDPE FT VIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVG FT QSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREF FT AKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEI FT QDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLL FT AYFLDKAAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPP FT EEALVTVPAHQ" XX SQ Sequence 3710 BP; 788 A; 1023 C; 1073 G; 826 T; 0 other; gcgtggtcgg aggccgggcc ctctcagctg gggcggagcc tactcactct ggggcggggc 60 ctgcgggaag atagacatga gctgtcgcgg cttggggagg ggcgtggccc aaggggcggg 120 acctcgggtg gcccgaggtg gcccggctgc tgggttggaa gcggcgtaat ccgactcatc 180 ccggccccgg gatggcgtcc ccacgggaat tgacccagaa ccccctgaag aagatctgga 240 tgccatacag caatgggcgg cccgctctgc acgcttgcca gcgcggtgtg tgcatgacca 300 actgcccaac gctcattgtc atggtgggcc tgcccgccag gggcaagacc tacatctcca 360 agaagctcac tcgatacctg aactggattg gtgtgcctac tcgggagttc aatgttggcc 420 agtatcgccg gaacatggtc aagacctaca aatcttttga attttttctc cccgacaatg 480 aagagggcct gaaaatcagg aagcagtgtg ccctggcagc tctccgtgac gtccggcgat 540 tccttagtga ggagggggga catgtggcgg tttttgatgc cacaaacacc acccgagaac 600 ggagagcgac catctttaat tttggagaac agaatggcta caagaccttt tttgtcgagt 660 ccatctgtgt ggatcctgag gtcatagctg ccaacatcgt gcaagtgaaa ctgggcagcc 720 ctgactatgt taaccgcgac agtgacgagg ctaccgagga cttcatgagg cgcattgagt 780 gctatgagaa ctcctacgag tcgctagatg aggacctgga tagggacctg tcctatatca 840 agatcatgga tgtgggtcag agctacgtgg tgaaccgtgt agctgaccac atccagagcc 900 gcatcgtata ttacctcatg aacatccacg tgaccccccg ctccatctac ctctgccggc 960 acggggagag tgagctcaac ctcaagggcc ggattggcgg ggacccagga ctgtcccccc 1020 ggggcaggga gtttgccaag agtctggccc agttcatcag tgaccagaat atcaaggatc 1080 tgaaggtctg gacaagccag atgaagagga caatccagac ggctgaggcg ctaggtgtgc 1140 cctatgaaca gtggaaggtc ctcaacgaga ttgacgcggg cgtctgtgag gaaatgacct 1200 atgaggaaat tcaggataat tatccactgg agttcgccct gcgggaccag gacaagtacc 1260 ggtaccggta ccctaaaggg gagtcctacg aggacctggt ccagagactg gagcctgtca 1320 tcatggagct ggagaggcaa gagaatgtgc tagtcatctg ccaccaggct gtgatgcgct 1380 gcctgctggc ctacttcctc gacaaggcag cagaacagct gccctacctc aagtgtccac 1440 tgcacacagt cctgaagctg acccctgtgg catacggttg taaagtggag tccatattcc 1500 tgaatgtggc cgctgtgaac acgcaccggg acaggcctca gaacgtggac atctcaagac 1560 ctccagagga agcccttgtc acggtgcctg ctcaccagtg accacgttca tccactgtga 1620 ccactaggca ggcactggtc tctgcagagg gggtcattcc aggccctcca gtgtgtgtga 1680 cagtcaccgt gccatgcagg gatattcttg aagccacata tggctggcgg aacccagaac 1740 ccccgcccca gcgcacctgg ctctttgttg acagtcggca acaaggttgt gcatggctcc 1800 tgacctgctg ctaagagtca cttgaccaga ctgcatctgc atgggctgtg cggaggttgc 1860 ccagccccag gttcttccgg cgcagctctt aggtgttcac tctcgccagc tcagttggct 1920 ttgtgaagtg tgaaacccta caatgtgaaa ggaaagtgct tgctgtgatg ttcctactgt 1980 ggcccagctg cccagcgtgg acctggtgac tctccgcagg gcctctacca tcctctctgt 2040 ggccactgcc tgagccagag gccaggtgtt catggggccc tgagcttctg ctgcctctgg 2100 taagagggag agcccttccc atccttaccc accaggaact agagccccaa ccacagcaga 2160 tgcttcccag gcagccactg gccaggccct tgtatccatg tcacccttat ttgcaggcat 2220 tcatggaagc aatgcgcttg cttcggcaca ttgcaatgca cagaaatgtg caggcagggg 2280 ggcagttgtg gtcgccccta ccctgttgtg catgtacccg gctacttagg tatgcttagg 2340 ggagctggtg gccagggatg ccccttcagg cctgctctca cagtaaaacc acctcaagac 2400 agggtaggca tacgagttcc cttcctggtg cacacaccga agtgctcgag ggagaggctg 2460 ccgccccctc tccccttccc tggctgtgaa cactggcagc cgatggggag gtggcctctc 2520 acctgttgct gcctgtgtcc acacaggaat gtacactggt ggcagagaca tgcatttgcc 2580 ataaatgatt cagaaacact tgtatggtcc tgtaaaactt ttctggtgtt ttttgacttc 2640 tgcctgtggg cctgagtaag ggagaaggca gacccatagg cagtccctgg gcaagggagg 2700 attttcccag ctgcagcaca gctttccatt catgtgcatg tgagtctggt gaaaatggcc 2760 ttgtgtagtc cagtgtctgt catgcccctg agttcttgtg gtcccagaga ggcagcctgg 2820 gcagtctgtt tacagctggc aaacactggc tggcaagttg tggtgctggg tcagaaagtg 2880 tctggactgg taatcgggtg ccctgctgtc cacaggtgga ctctggggaa gccaggcccc 2940 tggatcccaa gtcctttgtg tctggagcct tacctggcct gtgcctggaa cgtagcacat 3000 ctttccagcc tcccagggct ttgcagcctg cagatgccac ctgcccctga gagaagttag 3060 catccctggc cctggctggt ggtctctcat ccttgtgtgc tgctctccta agagatgtcc 3120 aaggcggagc aggggcagga tcctccttcc acactcatct gtcagagccc caaacccttt 3180 agacccacag cccaaggacc atgccactcc ctttggggca tggggactgc agcctttgct 3240 tctgtgtctt ttggagtttc ggtgactttt gtcacctgga cacactcaat tgttagccat 3300 agtgggtccc cttggtcagc aacagtgcat gtacctctgg atgtcacctg aggtgagacc 3360 accgaggcct tttctctcta tgtacagagg ggagttagga gctgctggac tggatgcatt 3420 acaaggactg gggacacggc agagggacat ccagggatca gggcatgagt ggggacagcc 3480 ccccggcctc tgccctggca tggtctccac atgggctgtg gcatggctga ttggctgcca 3540 catttcggcc atgtgggctg gtgtgcccat gttgcaggta ttttcccgag ttccccagaa 3600 tggatggtat cgaatctcag ccacatgcaa cactgtgtcc agcattcttt gcaataaata 3660 ctttttaaaa tataatctca ggttgtattt gataaaaaaa aaaaaaaaaa 3710 // |
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