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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB125971; SV 1; linear; genomic DNA; STD; PRO; 1647 BP.
XX
AC   AB125971;
XX
DT   10-MAY-2004 (Rel. 79, Created)
DT   03-AUG-2004 (Rel. 80, Last updated, Version 2)
XX
DE   Arthrobacter globiformis bff gene for beta-fructofuranosidase protein,
DE   complete cds.
XX
KW   .
XX
OS   Arthrobacter globiformis
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Micrococcaceae; Arthrobacter.
XX
RN   [1]
RP   1-1647
RA   Watanabe K.;
RT   ;
RL   Submitted (07-NOV-2003) to the EMBL/GenBank/DDBJ databases.
RL   Kenji Watanabe, Hokkaido University, Department of Applied Bioscience;
RL   North 9 west 9, Sapporo, Hokkaido 060-8589, Japan
RL   (E-mail:kenji55@chem.agr.hokudai.ac.jp, Tel:81-11-706-2500(ex.2500),
RL   Fax:81-11-706-3635)
XX
RN   [2]
RX   PUBMED; 16233623.
RA   Isono N., Tochihara T., Kusnadi Y., Win T.T., Watanabe K., Obae K., Ito H.,
RA   Matsui H.;
RT   "Cloning and heterologous expression of a beta-fructofuranosidase gene from
RT   Arthrobacter globiformis IFO 3062, and site-directed mutagenesis of the
RT   essential aspartic acid and glutamic acid of the active site";
RL   J. Biosci. Bioeng. 97(4):244-249(2004).
XX
DR   StrainInfo; 221575; IFO 3062.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1647
FT                   /organism="Arthrobacter globiformis"
FT                   /strain="IFO 3062"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1665"
FT   CDS             1..1647
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="bff"
FT                   /product="beta-fructofuranosidase protein"
FT                   /db_xref="GOA:Q75UJ7"
FT                   /db_xref="InterPro:IPR003469"
FT                   /db_xref="UniProtKB/TrEMBL:Q75UJ7"
FT                   /protein_id="BAD18121.1"
FT                   /translation="MERTCITVRAIVRFHIEQRQTIVNKQRTKRGILTAALSIGALGAT
FT                   LISGPAVAATDAAPGFPQPTEHTQKAYSPTDNFTSRWTRADAKQLKAMSDPDAGSRENS
FT                   MPTEYTMPTVSQDFPDMSNEKVWVWDTWPLIDENANQYSVNGQEIIFSLVADRKLGFDE
FT                   RHQYARIGYFYRPAGIPADERPEDGGWTYGGQVFDEGVTGKIFEDQSFTHQTQWSGSAR
FT                   VSKNGEIKLFFTDVAFYRDKDGQDVKPYDSRIALSVGHVHSNKKGVKLTGFNKVKELLQ
FT                   ADGKNYQNAAQNSYYNFRDPFTFVDPAHPGETYMVFEGNSAMDRDEAKCTAEDLGYREG
FT                   ETNGETVEQVNNSGATYQIGNVGLARAKNKALTEWEFLPPILSANCVTDQTERPQIYMQ
FT                   DGKYYLFTISHRSTFATGIDGPEGVYGFVGNGIRSDYQPLNRGSGLALGSPTNLNFAAG
FT                   TPFAPDYNQHPGQFQAYSHYVMPGGLVQSFIDTIGTKDNFVRGGTLGPTVKLNIKGDSA
FT                   TVDYNYGDNGLGGWADIPANRELKNSKAVAK"
XX
SQ   Sequence 1647 BP; 418 A; 487 C; 424 G; 318 T; 0 other;
     gtggagagaa cgtgtatcac ggtcagagcg attgtccgat tccatatcga acaaaggcaa        60
     acaatcgtga acaaacaacg tacgaagcgt ggcatcctta ctgccgcact gagcatcggg       120
     gcacttggtg ctaccttgat ctcggggcca gctgtagctg ccaccgacgc agcacctggc       180
     ttcccacagc caaccgagca cactcagaag gcttacagcc caaccgacaa tttcacctcg       240
     cgttggactc gtgctgatgc aaagcagctc aaggccatgt ctgatccaga tgccggttcg       300
     cgtgaaaatt ccatgcctac ggaatacacc atgccgaccg tttctcagga tttccctgac       360
     atgtccaacg aaaaggtttg ggtctgggat acctggccac tgatcgacga aaacgctaac       420
     cagtacagcg tcaacggtca ggaaatcatc ttctcgttgg ttgccgaccg caagttgggc       480
     ttcgatgagc gtcaccagta cgcacgcatc ggctacttct accgcccagc aggaatccct       540
     gctgacgaac gtcccgaaga tggcggctgg acctacggcg gacaggtctt cgacgaaggc       600
     gtgaccggga agatctttga agatcagtct ttcacccacc agacccagtg gtcgggctcc       660
     gcacgcgtaa gcaagaacgg tgaaattaag ctcttcttca ccgacgttgc tttctaccgc       720
     gacaaggacg ggcaggacgt caagccttat gactcacgca tcgcattgag cgttggtcac       780
     gtccactcga acaaaaaggg cgtgaagctg accggcttca acaaggtcaa ggaattgctg       840
     caggcagacg gtaagaacta ccagaatgct gcgcagaact cctactacaa cttccgcgac       900
     ccattcacct ttgtagaccc agcgcaccca ggcgagacct acatggtctt tgaaggcaac       960
     tcggccatgg atcgcgacga ggccaagtgc accgctgaag atcttggtta ccgcgaaggc      1020
     gaaaccaacg gtgaaaccgt tgagcaagtc aacaactccg gtgcaaccta ccagatcggt      1080
     aacgttggac tggctcgtgc caagaacaag gcactgactg aatgggaatt cctgccacca      1140
     atcttgtcgg ctaactgcgt taccgaccag actgagcgtc cgcagatcta catgcaggat      1200
     ggcaagtact acctgttcac cattagccac cgttcgactt tcgccaccgg cattgacggt      1260
     ccagaaggcg tctacggatt tgttggtaac ggcatccgca gtgactacca gccactgaac      1320
     cgtggatcgg gtctggcact tggcagccca acgaacctga acttcgcagc aggtacccca      1380
     ttcgcacctg actacaacca gcacccaggc cagttccagg catactcgca ctacgtgatg      1440
     ccaggcggtc tagttcagtc cttcatcgat accattggca ccaaggacaa ctttgttcgc      1500
     ggtggcacct tgggcccaac cgttaagctc aacatcaagg gtgactcggc aaccgtcgac      1560
     tacaactacg gcgataacgg tcttggcgga tgggcagata tcccagccaa ccgcgagctg      1620
     aagaacagca aggcagtagc caagtaa                                          1647
//
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