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EBI DbfetchID AB103384; SV 1; linear; genomic DNA; STD; PRO; 2282 BP. XX AC AB103384; XX DT 19-AUG-2003 (Rel. 76, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Geobacillus stearothermophilus ptb, leudh, bk genes for DE phosphoytansbutyrylase, leucine dehydrogenase, butyrate kinase, partial and DE complete cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-2282 RA Nagata S.; RT ; RL Submitted (16-FEB-2003) to the EMBL/GenBank/DDBJ databases. RL Shinji Nagata, Kochi University, Department of Bioresources Science; Monobe RL B200, Nankoku, Kochi 783-8502, Japan (E-mail:nagashin@cc.kochi-u.ac.jp, RL Tel:81-88-864-5190, Fax:81-88-864-5200) XX RN [2] RA Katoh R., Seki M., Nagata S., Misono H.; RT "Nucleotide sequence, cloning, overexpression and sigt-directed mutagenesis RT of the leucine dehydrogenase gene from Bacillus sphaericus"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..2282 FT /organism="Geobacillus stearothermophilus" FT /mol_type="genomic DNA" FT /note="synonym: Bacillus stearothermophilus" FT /db_xref="taxon:1422" FT CDS <1..708 FT /codon_start=1 FT /transl_table=11 FT /gene="ptb" FT /product="phosphoytansbutyrylase" FT /db_xref="GOA:Q76GN8" FT /db_xref="InterPro:IPR002505" FT /db_xref="UniProtKB/TrEMBL:Q76GN8" FT /protein_id="BAC81828.1" FT /translation="GELDIVHTNSVPQAARNCVRPVHWNEADALMKGHVPTATLVKAVL FT NKEYGLRTGKVLSHVAVFDVPAFERLIIVTDAAMNIAPDLEQKVQIVNNAVSVARSIGI FT ERPKVAALAAVETVNPAMPATVDAAALAMMQKRGQIEHCLLDGPLALDNAVSMTAAKHK FT RIESEVAGQADILLVPDIESGNMLYKSLVYFAKAKVGAVIAGAKAPIVLTSRADSAESK FT LYSLALAICSAAK" FT CDS 729..1832 FT /codon_start=1 FT /transl_table=11 FT /gene="leudh" FT /product="leucine dehydrogenase" FT /db_xref="GOA:P13154" FT /db_xref="InterPro:IPR006095" FT /db_xref="InterPro:IPR006096" FT /db_xref="InterPro:IPR006097" FT /db_xref="InterPro:IPR016040" FT /db_xref="InterPro:IPR016211" FT /db_xref="UniProtKB/Swiss-Prot:P13154" FT /protein_id="BAC81829.1" FT /translation="MELFKYMETYDYEQVLFCQDKESGLKAIIAIHDTTLGPALGGTRM FT WMYNSEEEALEDALRLARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEAMFRAFGRFIQ FT GLNGRYITAEDVGTTVADMDIIYQETDYVTGISPEFGSSGNPSPATAYGVYRGMKAAAK FT EAFGSDSLEGKVVAVQGVGNVAYHLCRHLHEEGAKLIVTDINKEVVARAVEEFGAKAVD FT PNDIYGVECDIFAPCALGGIINDQTIPQLKAKVIAGSADNQLKEPRHGDIIHEMGIVYA FT PDYVINAGGVINVADELYGYNRERAMKKIEQIYDNIEKVFAIAKRDNIPTYVAADRMAE FT ERIETMRKARSPFLQNGHHILSRRRAR" FT CDS 1887..>2282 FT /codon_start=1 FT /transl_table=11 FT /gene="bk" FT /product="butyrate kinase" FT /db_xref="GOA:Q76GN6" FT /db_xref="InterPro:IPR000890" FT /db_xref="InterPro:IPR011245" FT /db_xref="UniProtKB/TrEMBL:Q76GN6" FT /protein_id="BAC81830.1" FT /translation="MQEQKFRILTINPGSTSTKIGVFENERPLLEKTIRHEAEVLRQYK FT TIADQYEFRKQTILQALDEEGINLSKLNAVCGRGGLLRPIEGGTYRVNEAMLEDLRRGY FT SGQHASNLGGILAHEIASALNIPAFIVD" XX SQ Sequence 2282 BP; 571 A; 616 C; 672 G; 423 T; 0 other; ggagagcttg acatcgttca caccaattcg gtcccccaag cggcccgaaa ctgcgtgcgc 60 cccgttcatt ggaatgaagc agatgcctta atgaaagggc acgtaccgac ggcaaccttg 120 gtgaaagccg tcttgaacaa agaatacggg ctgcgcacag gaaaggtgtt gtcccatgtc 180 gcggtgtttg acgtgccggc gttcgaacgg ctgatcatcg tgaccgacgc ggccatgaac 240 atcgcccccg atctcgagca aaaagtgcaa attgtgaaca atgccgtcag cgtggcgcgt 300 tccatcggca tcgagcgtcc gaaagtcgcg gcgctggcgg cggtggagac ggtcaatccg 360 gccatgccgg caaccgtcga tgcagcggcg ctcgcgatga tgcaaaagcg gggacaaatc 420 gaacattgcc tgcttgacgg gccgcttgcc ttggacaatg cagtgtctat gacggcggcg 480 aagcataaac gaattgaaag cgaagtggct ggacaggctg atattttgct cgtgccggac 540 attgaatccg gcaacatgtt gtacaagtcg ctcgtctact ttgcaaaagc caaagtcggc 600 gcggtcatcg ccggggcgaa ggcgccgatc gtcttgacgt cgcgcgccga ctcggcggaa 660 agcaaactgt attcgttggc gctcgccatc tgttcggcag cgaaatgaaa agaaggaggc 720 catctgagat ggaattgttc aaatatatgg aaacttacga ttatgagcaa gtgctgtttt 780 gccaagataa agaatcgggt ttgaaagcga tcattgccat tcatgacaca acgctcggcc 840 cggcgctcgg cgggacgcgc atgtggatgt acaattcgga agaagaagcg cttgaagacg 900 ccttgcgcct cgcccgcggc atgacgtaca aaaacgcggc cgccggcctc aacttgggcg 960 ggggcaaaac ggtcatcatc ggcgacccgc gcaaagataa aaacgaagcg atgttccggg 1020 cgttcggccg cttcattcaa gggctgaacg gccgctacat cacggcggaa gacgtcggca 1080 cgaccgtcgc cgatatggat atcatctatc aagaaaccga ctatgtcacc ggcatttcgc 1140 ccgaattcgg ctcatccggc aacccatcgc cggcgaccgc ctacggcgta taccgcggca 1200 tgaaggcggc ggcaaaagag gcgttcggca gcgattcgct cgaaggaaaa gtcgtcgccg 1260 tccaaggagt cggcaatgtc gcgtatcatt tgtgccgcca tttgcacgaa gaaggagcga 1320 aactcatcgt gactgacatc aacaaggaag tggtggcgcg cgcggtcgag gaattcggag 1380 cgaaagcggt cgacccgaac gacatttacg gcgtggagtg cgacattttt gctccatgcg 1440 cgctcggcgg catcatcaac gatcaaacga ttccgcaact gaaagcgaaa gtgatcgccg 1500 gatcggcaga caaccagctg aaagagccgc gccatggcga catcatccat gaaatgggca 1560 tcgtctatgc cccggattat gtgatcaacg ccggcggcgt catcaacgtc gcggacgaac 1620 tgtacggcta caatcgggaa cgggcgatga aaaaaatcga gcaaatttat gacaacatcg 1680 aaaaagtgtt tgccatcgcc aagcgcgaca acattccaac gtatgtggcc gccgaccgga 1740 tggcggaaga acggattgaa acgatgcgca aagcgcgcag tccatttttg caaaatggtc 1800 accatatttt aagccgccgt cgcgcccgct aacagcggcg cggggcggcc tccgccccgc 1860 cggcggatga cggagggacg acaacgatgc aagagcagaa gttccgcatt ttaacgatca 1920 atccaggttc gacgtcaacg aaaattggcg tctttgaaaa cgaacggcca ttgctcgaaa 1980 aaacgatccg ccacgaggcg gaggtgcttc ggcaatataa aacgattgcg gatcagtatg 2040 agttccgcaa acaaacgatt ttgcaagctc tcgatgaaga agggatcaat ttatcgaagt 2100 tgaacgccgt ctgcggccgc ggtggactcc tccgcccgat cgaaggcggg acgtaccgcg 2160 tcaatgaggc gatgcttgaa gatttgcgcc gcggttattc gggacagcac gcctccaatc 2220 tcggcggcat tttggcgcat gagatcgcct cggccttaaa catcccggcg tttatcgtcg 2280 ac 2282 // |
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