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EBI DbfetchID AB088066; SV 1; linear; genomic DNA; STD; PRO; 6739 BP. XX AC AB088066; XX DT 12-JUL-2002 (Rel. 72, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus subtilis biotin operon (bioW, bioA, bioF, bioD, bioB, bioI), DE complete cds. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6739 RA Kurusu Y., Sasaki M., Kotanagi T.; RT ; RL Submitted (10-JUL-2002) to the EMBL/GenBank/DDBJ databases. RL Yasurou Kurusu, School of Agriculture, Ibaraki University, Laboratory of RL Molecular Microbiology; 3-21-1, Chuo, Ami, Inashiki, Ibaraki 300-0393, RL Japan (E-mail:krsy@ipc.ibaraki.ac.jp, Tel:81-298-88-8646) XX RN [2] RX DOI; 10.1271/bbb.68.739 RX PUBMED; 15056910. RA Sasaki M., Kawamura F., Kurusu Y.; RT "Genetic analysis of an incomplete bio operon in a biotin auxotrophic RT strain of Bacillus subtilis natto OK2"; RL Biosci. Biotechnol. Biochem. 68(3):739-742(2004). XX FH Key Location/Qualifiers FH FT source 1..6739 FT /organism="Bacillus subtilis" FT /isolate="OK2" FT /mol_type="genomic DNA" FT /note="synonym:Bacillus natto" FT /db_xref="taxon:1423" FT CDS 561..1238 FT /codon_start=1 FT /transl_table=11 FT /gene="bioW" FT /product="6-carboxyhexanoate-CoA ligase" FT /db_xref="GOA:Q8KZN1" FT /db_xref="InterPro:IPR005499" FT /db_xref="UniProtKB/TrEMBL:Q8KZN1" FT /protein_id="BAC03239.1" FT /translation="MMQEETFYSVRMRASMNGSHEDGGKHISGGERLIPFHEMKHTVNA FT LLEKGLSHSRGKPDFMQIQFEEVHESIKTIQPLPVYTNEVSCPGEGQKLARLLLEKEGV FT SRDVIEKAYEQIPEWSDVRGAVLFDIHTGKRMDQTKEKGVRVSRMDWPDANFEKWALHS FT HVPAHSRIKEALALASKVSRHPAAIAELCWSDDPDYITGYVAGKKMGYQRITAMKEYGT FT EEG" FT CDS 1330..2676 FT /codon_start=1 FT /transl_table=11 FT /gene="bioA" FT /product="adenosylmethionine-8-amino-7-oxononanoate FT aminotransferase" FT /db_xref="GOA:Q8KZN0" FT /db_xref="HSSP:1QJ3" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/TrEMBL:Q8KZN0" FT /protein_id="BAC03240.1" FT /translation="MTHDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVQDINGKE FT YYDGFSSVWLNVHGHRKKELDNAIKKQLGKIAHSTLLGMTNVPATQLAETLIDISPKKL FT TRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIELFH FT HVYGPLMFESYKAPIPYVYRSESGDPDECCDQCLRELEQLLKEHHEEIAALSIESIVQG FT ASGMIVMPEGYLAGVRELCTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKG FT ITGGYLPIAVTFATEDIYKAFYDDYENLKTFFHGHSYTGNQLGCAVALENLALFESENI FT VEQVAEKSKKLHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVS FT LKMRELGMLTRPLGDVIAFLPPLASTAEELTEMVAIMKQAIHEVTSLEN" FT CDS 2666..3781 FT /codon_start=1 FT /transl_table=11 FT /gene="bioF" FT /product="8-amino-7-oxononanoate synthase" FT /db_xref="GOA:Q8KZM9" FT /db_xref="HSSP:1FC4" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:Q8KZM9" FT /protein_id="BAC03241.1" FT /translation="MKIDSWLNDRLDIAKEAGVHRNLRSMNGAPVPERNIDGENQTVWS FT SNNYLGLASDRRLIDAAQTALQQFGTGSSGSRLTTGNSVWHEKLEKKIASFKRTEAALL FT FSSGYLANVGVLSSLPEKEDVILSDQLNHASIIDGCRLSKADTVVYRHIDMNDLENKLN FT ETQRYQRRFIVTDGVFSMDGTIVPLDQIISLAKRYHAFVVVDDAHATGVLGDSGRGTSE FT YFGVYPDIVIGTLSKAVGTEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAF FT NIRTSLKNMGYVVKGDHTPIIPVVIGDAHKTVIFAEKLQGKGIYAPAIRPPTVAPGESR FT IRITITSDHSMGDIDDLLKTFHSIGKELHII" FT CDS 3778..4473 FT /codon_start=1 FT /transl_table=11 FT /gene="bioD" FT /product="dethiobiotin synthetase" FT /db_xref="GOA:Q8KZM8" FT /db_xref="HSSP:1BYI" FT /db_xref="InterPro:IPR004472" FT /db_xref="UniProtKB/Swiss-Prot:Q8KZM8" FT /protein_id="BAC03242.1" FT /translation="MRGFFVTGTDTEVGKTVISSGLAALLKEHNRDVGVYKPFLSGISR FT HHPDSDTSLLKDMSQTSLSHEDITPFAFKAPLAPYVAGKLEGKTVTMEEVLSHWGRIRE FT KHECFIVEGAGGISVPLGEDYLVSHVIKALQLPMIIVARPHLGTINHTFLTVKYAESMG FT LPIAGIIINGISDSPDEDEKTNPEMIERLCGVPILGVTPKLANVTKETVLHMVKDHINL FT SLLMNQMGV" FT CDS 4476..5483 FT /codon_start=1 FT /transl_table=11 FT /gene="bioB" FT /product="biotin synthetase" FT /db_xref="GOA:Q8KZM7" FT /db_xref="HSSP:1R30" FT /db_xref="InterPro:IPR007197" FT /db_xref="UniProtKB/Swiss-Prot:Q8KZM7" FT /protein_id="BAC03243.1" FT /translation="MNQWMELANRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRK FT HFYGKKVKLNMIMNAKSGLCPENCGYCSQSAISKAPIESYRMVNKETLLEGAKRAHDLN FT IGTYCIVASGRGPSNREVDQVVDAVQEIKETYGLKVCACLGLLKPEQAKRLKDAGVDRY FT NHNLNTSQRNHSNITTSHTYDDRVNTVEIAKESGLSPCSGAIIGIKETKQDVIDIAKSL FT KALDADSIPVNFLHAIDGTPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLR FT SLQPLGLYAANSIFVGDYLTTAGQEETEDHKMLSDLGFEVESVEEMKASLSAKS" FT CDS 5552..6739 FT /codon_start=1 FT /transl_table=11 FT /gene="bioI" FT /product="cytochrome P450 enzyme" FT /db_xref="GOA:Q8KZM6" FT /db_xref="HSSP:1IO7" FT /db_xref="InterPro:IPR017973" FT /db_xref="UniProtKB/TrEMBL:Q8KZM6" FT /protein_id="BAC03244.1" FT /translation="MTIASSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGY FT EETAAILKDARFKVRTPLPESSTKYQDLSHVQNQMMLFQNQPDHRRLRTLASGAFTPRA FT TESYQPYIIETVHQLLDQVQGEKKMEVISDFAFPLASFVIANIIGVPEEDREQLKEWAA FT SLIQTIDFTRSRKVLTEGNHMAVQAMAYFKELIQKRKRHPQQDMISMLLKGKENDKLTE FT EEAASTCILLAIAGHETTVNLISNSVLCLLQHPEQLLKLRENPDLIGTAVEECLRYESP FT TQMTARVASEDIDISGVTIRQGEQVYLLLGAANRDPSIFTNPDVFDITRSPNPHLSFGH FT GHHVCLGSSLARLEAQIAINTFLQRMPSLKLADFEWRYRPLFGFRALEELPVTFE" XX SQ Sequence 6739 BP; 1963 A; 1372 C; 1692 G; 1712 T; 0 other; gaccggatta aggtgtgtgt ggatttgtgc agccaggtgg atcatcacgt gctgatcaaa 60 acacagaatc ttgacaggca cggcttttat tattacgtcc catcgcttgc aaagcatttt 120 tccgcatcgg agatccaaag cctgatcgcg ccgcgcccgc accttagtct tgttggtgta 180 cacgatcggt taacgccggc tgagggcgtg gacaaaatcg aaaaagaatt gacagctgtc 240 tatgctggac agggagctgc tgattgctac cgagtggtcc gttctgcttc gggacatttc 300 gaaacagcag ttatgaggca tgaagctgtc cggtttttgc aaaagtggct gtgactgtaa 360 aaagaaatcg aaaaagaccg ttttatgtga aaacggtctt tttgtatcct tttaaccaac 420 tgccataaat cgatcctttc ttctattgac agaaacagga gagaatatta tattctaatt 480 gttaaccttt aaatataatt ggttaacaat tgaggtgaga agcgctacac gttcttcagt 540 tatcagtgaa agggcgagaa atgatgcaag aagaaacttt ttatagtgtc agaatgaggg 600 cttctatgaa tgggtctcat gaagacggcg gaaagcatat atccggcgga gaacggctta 660 ttcctttcca tgagatgaag catacagtca atgctttatt agaaaaaggg ttatcccatt 720 caagaggaaa acctgatttt atgcaaattc aatttgaaga ggtacatgaa tcgataaaaa 780 ccattcagcc attgcctgtg tatacgaatg aagtgagctg cccgggagaa ggacaaaagc 840 ttgcccgatt gttattggaa aaagaaggcg tttcacgaga cgtgattgaa aaagcgtatg 900 aacaaatccc agaatggtca gatgtcaggg gtgcggtgtt gtttgatatt catacaggca 960 agcgaatgga tcaaacaaaa gaaaaagggg tgcgggtctc cagaatggat tggccggacg 1020 ctaattttga aaaatgggcg cttcacagtc acgtgccggc tcattcaaga ataaaagagg 1080 cccttgcgct cgcttcaaag gtaagccggc acccggcagc cattgcagaa ttatgctggt 1140 cggacgatcc ggattacata acaggctatg ttgcgggtaa gaaaatgggc tatcagcgta 1200 ttacagcaat gaaagaatac gggactgaag agggctgacg agtctttttt attgatggat 1260 ccaatgatgt aaacacgtac atacatgatc tggagaagca gcctatttta atagagtggg 1320 aggaagacca tgactcatga tttgatagaa aaaagtaaaa agcacctctg gctgccattt 1380 acccaaatga aagattatga tgaaaacccc ttaatcatcg aaagcgggac tggaatcaaa 1440 gtccaagaca taaatggcaa ggaatactat gacggttttt catcagtttg gcttaatgtc 1500 cacggacacc gcaaaaaaga actagataac gccataaaaa aacagctcgg aaaaattgcg 1560 cactccacgt tgttgggcat gaccaacgtt ccagcaaccc agcttgccga gacattaatc 1620 gacatcagcc caaaaaagct cacgcgggtc ttttattcag acagcggtgc agaagcgatg 1680 gaaatagcct taaaaatggc gtttcagtat tggaagaaca ttgggaagcc cgagaaacaa 1740 aaatttatcg ctatgaaaaa cgggtatcac ggtgatacga ttggcgccgt cagtgtcggt 1800 tcaattgagc tttttcacca cgtatacggc ccgttgatgt tcgagagtta caaggcaccg 1860 attccttatg tgtatcgctc tgaaagcggt gatcctgatg agtgctgtga tcagtgcctc 1920 cgagagcttg aacagctgct taaagaacat cacgaggaaa ttgccgcgct ttccattgaa 1980 tcaatagtac aaggcgcgtc cggtatgatc gtgatgccgg aaggatattt ggcaggggtg 2040 cgcgagctat gtacaacata cgatgtctta atgatcgttg atgaagtcgc tacaggcttt 2100 ggccgtacag gaaaaatgtt tgcgtgcgag cacgagaatg tccagcctga tctgatggct 2160 gccggtaaag gcattacagg aggctatttg ccaattgccg ttacgtttgc cactgaagac 2220 atctataagg cattctatga tgattatgaa aacctaaaaa cctttttcca tggccattcc 2280 tatacaggca atcagcttgg ctgtgcggtt gcgcttgaaa atctggcatt atttgaatct 2340 gaaaacattg tggaacaagt agcggaaaaa agtaaaaagc tccattttct tcttcaagat 2400 ctgcacgctc ttcctcatgt tggggatatt cggcagcttg gctttatgtg cggtgcagag 2460 cttgtccggt caaaggaaac caaagaacct tacccggctg atcggcggat tggatacaaa 2520 gtttccttaa aaatgagaga gttaggaatg ctgacaagac cgcttgggga cgtgattgca 2580 tttctgcctc ctctcgccag cacagctgaa gagctcacgg aaatggttgc cattatgaaa 2640 caagcgatcc acgaggttac gagccttgaa aattgattcc tggttaaatg accggttaga 2700 catagcaaag gaagccggcg tacaccgtaa cctgcggtca atgaatgggg caccggttcc 2760 ggagaggaac attgatggcg aaaatcaaac ggtctggtcc tcaaacaatt atttagggct 2820 cgcaagcgat agacgtttga tcgatgcagc ccaaacagca ttgcagcaat ttgggacagg 2880 aagtagcggt tcacgtttaa cgacaggcaa ttcggtctgg catgaaaagc tagaaaagaa 2940 gattgccagc tttaagcgga cagaagcggc cctgctgttt tcgagcggtt acttggccaa 3000 tgtcggtgtc ctttcatcct tgccagaaaa ggaagatgtc attttaagtg accagctcaa 3060 tcatgcaagt attatcgacg gctgccgact ttctaaggct gatacagttg tttatcggca 3120 tattgatatg aatgatcttg aaaacaagct gaatgaaaca cagcgttatc agcgccgttt 3180 tatcgtaaca gacggggtat tcagcatgga tggcacaatc gtccctcttg atcagatcat 3240 ctcacttgcg aaacgctatc atgccttcgt ggtcgttgat gacgcccacg caacaggagt 3300 tttaggtgat tcgggacgag gaacgagtga atactttggt gtttatcctg acattgtgat 3360 cggcacctta agcaaagctg ttggtacgga aggaggtttt gcggctggat cagcggtctt 3420 catcgacttt ttgctgaacc atgccagaac atttatcttt caaaccgcta ttccgccagc 3480 cagctgtgcg gctgctcacg aggctttcaa catcagaacc agtttgaaga atatgggtta 3540 tgtggtgaaa ggagatcaca caccgattat tcctgtagtc attggcgatg cccataaaac 3600 ggtcatattt gctgaaaaac tgcagggcaa gggaatttat gctcctgcca ttcggccgcc 3660 gaccgttgcg ccgggtgaaa gccgtattcg aattacaatc acgtctgacc acagtatggg 3720 tgatattgat gatttgctga aaacatttca ttcaatcgga aaggagctgc atatcatttg 3780 aggggttttt ttgtgacggg aactgataca gaagtaggga aaacggttat atccagcggt 3840 cttgctgctt tattgaaaga acataataga gatgttgggg tgtataaacc atttttaagc 3900 gggatatcac gccatcatcc agatagtgat actagtctgc tgaaagacat gtcgcagacc 3960 agtctttctc atgaagacat tacgcctttt gccttcaagg cgccgcttgc accatacgtt 4020 gcagggaaac ttgagggaaa gactgtcacc atggaagagg ttttaagcca ttgggggcgg 4080 attagagaaa aacatgaatg cttcatcgta gaaggtgcgg gcggtatttc tgtgccattg 4140 ggagaggact atttggtcag tcatgtcata aaagcgttgc agcttcccat gattattgtg 4200 gcgcgtcctc accttggaac cattaatcat acctttttaa ctgtcaaata tgcagaaagc 4260 atggggcttc caattgccgg aattatcatc aatggaatca gtgactctcc tgatgaagat 4320 gaaaaaacca atcctgagat gattgagcgc ttatgcggtg ttccgatttt aggggttacg 4380 ccaaagcttg ccaacgttac gaaagaaacg gttctacata tggtaaaaga ccatatcaat 4440 ctatcattac tgatgaatca aatgggggta tgagaatgaa tcaatggatg gaactcgcaa 4500 accgggtgct ggctggagca gaagtgactg acgaagaggc gctttcaata ttacattgtc 4560 ctgatgaaga tattttgcta ttaatgcatg gagcttttca tatcagaaaa cacttttatg 4620 gaaaaaaagt aaagctcaat atgattatga atgcgaaatc cgggctctgc ccggaaaact 4680 gcggctattg ttcacagtct gcgatttcga aagcgccgat tgagtcttat cggatggtga 4740 ataaggaaac gctgcttgaa ggcgcgaagc gggcgcacga tctgaatatc ggcacatatt 4800 gtatcgtggc aagcggcaga gggccgtcta acagagaagt ggatcaggtc gtagatgcgg 4860 ttcaggaaat taaagagacg tatggactga aggtttgtgc gtgtctcgga ctgttgaagc 4920 cagagcaggc gaagcggctc aaagatgccg gagtagaccg ctataatcat aatttgaata 4980 cgtcacagag gaaccattca aacatcacga cctcacatac atacgatgac agagtcaata 5040 cggttgaaat cgcaaaagaa tcggggctgt ctccgtgttc aggcgccatt atcgggatta 5100 aggagacgaa acaggatgtc attgacatcg ccaaaagctt gaaggccctt gacgcggatt 5160 ccattcctgt gaattttttg catgcaattg atggcacgcc gttagaaggc gtcaacgaat 5220 taaacccgct gtattgttta aaagtgctgg cgctgttccg ttttatcaat ccatcaaaag 5280 aaattcgcat ttccggagga agagaggtca atctccgctc attgcagcca ttagggcttt 5340 acgccgcaaa ctccattttt gtcggagact acttaacaac tgccgggcaa gaggagacgg 5400 aggatcataa aatgctgagt gatttaggct ttgaagttga atcagtcgaa gaaatgaagg 5460 ccagtttaag tgcgaaaagc tgaaagaatc aataaaagca atcggtatga tgtcgattgc 5520 ttttattttt gaacagaaag gagaaaatca cgtgacaatt gcatcgtcaa ctgcatcttc 5580 tgagtttttg aaaaacccat attcctttta cgacacattg cgagctgttc atcctatcta 5640 taaagggagt ttcttaaaat acccgggctg gtatgtcaca ggatatgaag aaacggctgc 5700 tattttgaaa gatgcgagat tcaaagtccg caccccgctg cctgagagct caaccaaata 5760 tcaggacctt tcacatgtgc aaaatcaaat gatgctgttt cagaaccagc ctgatcatag 5820 acgattgcgg acgcttgcca gcggagcgtt tacgccgaga gcgacagaga gttatcagcc 5880 gtatatcatt gaaactgtcc accaattgct tgatcaagtg caaggtgaaa aaaagatgga 5940 ggtcatttcg gactttgctt ttcctttagc aagctttgtc atcgctaaca ttataggtgt 6000 accggaggaa gatagggagc aattaaagga gtgggctgcg agtctcattc aaacgattga 6060 ttttacccgc tcaagaaagg tattaacaga gggcaatcat atggctgtgc aggctatggc 6120 atatttcaaa gagctgattc aaaagagaaa acgccaccct caacaggata tgatcagcat 6180 gctcttgaag gggaaagaaa atgataagct gacggaagag gaggcggcat ctacgtgcat 6240 attgctggcg atcgccggac atgagacaac ggttaatctc atcagcaatt cagtcctttg 6300 tctgctgcag catccagaac agcttttgaa actgagagaa aatccagatc ttattggtac 6360 cgcagttgag gaatgtttac gctatgaaag ccccacgcaa atgacagcca gagttgcgtc 6420 agaggatatt gacatcagcg gggtgacgat ccgtcaagga gagcaagtct atcttttgtt 6480 aggagcggct aatcgagacc ctagcatatt cacgaacccc gatgtcttcg atattacgag 6540 aagtcctaat ccgcaccttt cattcgggca tggccatcat gtttgcttag ggtcctcgct 6600 ggcacgatta gaagcgcaaa ttgcgattaa cacctttctg cagcgaatgc ccagccttaa 6660 gctcgcggat tttgaatggc ggtatcggcc gctttttgga tttcgggcgc ttgaggagct 6720 gccggtgact tttgaataa 6739 // |
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