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EBI DbfetchID AB086444; SV 1; linear; genomic DNA; STD; PRO; 3969 BP. XX AC AB086444; XX DT 21-DEC-2002 (Rel. 74, Created) DT 12-AUG-2003 (Rel. 76, Last updated, Version 3) XX DE Geobacillus stearothermophilus inuA gene for exo-inulinase, complete cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-3969 RA Tsujimoto Y., Watanabe A., Nakano K., Watanabe K., Matsui H., Suzuki Y.; RT ; RL Submitted (17-JUN-2002) to the EMBL/GenBank/DDBJ databases. RL Yoshiyuki Tsujimoto, Kyoto Prefectural University, Department of Applied RL Biochemistry; Shimogamo. Sakyo, Kyoto, Kyoto 606-8522, Japan RL (E-mail:yoshi_t@kpu.ac.jp, Tel:81-75-703-5669, Fax:81-75-703-5669) XX RN [2] RX DOI; 10.1007/s00253-003-1261-3 RX PUBMED; 12883863. RA Tsujimoto Y., Watanabe A., Nakano K., Watanabe K., Matsui H., Tsuji K., RA Tsukihara T., Suzuki Y.; RT "Gene cloning, expression, and crystallization of a thermostable RT exo-inulinase from Geobacillus stearothermophilus KP1289"; RL Appl. Microbiol. Biotechnol. 62(2-3):180-185(2003). XX FH Key Location/Qualifiers FH FT source 1..3969 FT /organism="Geobacillus stearothermophilus" FT /strain="KP1289" FT /mol_type="genomic DNA" FT /note="synonym:Bacillus stearothermophilus" FT /db_xref="taxon:1422" FT CDS 837..2318 FT /codon_start=1 FT /transl_table=11 FT /gene="inuA" FT /product="exo-inulinase" FT /EC_number="3.2.1.80" FT /db_xref="GOA:Q8GI55" FT /db_xref="InterPro:IPR019758" FT /db_xref="UniProtKB/TrEMBL:Q8GI55" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAC45010.1" FT /translation="MKTHNSEKYRPTFHFSPKKNWMNDPNGLVYFKGEYHLFFQHNPND FT SIWGPMHWGHAVSKDMIEWEELDIALYPDENGTIFSGSVVIDWNNTSGFFPKEPGMVAI FT FTQNLHDADHTTPIQTQSLAFSHDRGRTWTKYEKNPVLKHPTKVDFRDPKVFWHYESEK FT WIMVLATGQTVSIYSSPNLIDWQFESEFGENIGCHDGVWECPDLFELPVENSEEKKWVM FT FVSIGDNRQFDSGSRTQYFIGSFDGSNFKVDENNNEVLWLDFGKDNYAGVTFSDIPKED FT GRRIYIGWMSNWRYANEVPTVGWRGQMTLPRVLSLRKIGDKLRVIQNPVKELDNYFTKQ FT IVIHDEIISSGIQKKIDINQPSVEIVLNVNNLGAKQFGLVLHHTENQSTTITIDLLTNL FT LSLDRTNSGEVSFSENFLNNQVMKIHDTTKLNLRIIVDSSSVEIFVNEGAHALTSLIYP FT DKACEKISIFSLGGDIQLSNSYISFPNSIRRNFGY" XX SQ Sequence 3969 BP; 1405 A; 584 C; 787 G; 1193 T; 0 other; ctgcagcaca agagggttac aaagaagcga taaaatatct ttctgaatta tataaggaag 60 gtttaattga tgaagaagcc tttgaacaag atttcaatac ttatgttgct aaaggaaagg 120 ctgggagata tggtttatat ttcacatggg ataaagcaaa tattaacagg tatgaatgac 180 aaatatgatt tgatgcctcc actggcagga ccaacaggag aaattaatgt tacacgtaca 240 aacggtatgg gacttgaccg aggaagaatg gttattacga gtgcaaataa aaatttagag 300 ctaacagcca aatggatcga tcagctttat gagccttctc aatctgttca aaataactgg 360 ggaacttatg gtgatacaaa gcagcaaaat atatttgaac tgggacaaaa aaacaggaat 420 ggttaaaaca tcttccctct taacgggact ggtccacttg aattgagaga aaaacaagcg 480 ttggaggacc attagctatt cttgatgaat actatggtaa agttacgaca atgccggatg 540 atgctgcttg gagattggat ttgatgaaaa aagttatggt ccctcatatg aaagcagaca 600 atatttatcc aaaaatattc ttctctttag atgaactaaa agaattatca aaaattgaaa 660 cagatttatt ctcttacgtg aacagaaaac gtgctgagtg gattaaaaac ggaaaagttg 720 aagaagaatg gcctgaatat ttagctgaat taaaaagact tggattgaat agatggcttg 780 aaattaaaca aagtggatac gacagatttt ataaatagtt aagggggata taacaattga 840 agactcataa ttcagaaaaa tacagaccaa catttcattt ttcaccaaaa aaaaattgga 900 tgaatgatcc aaacggtcta gtatatttca aaggtgaata tcatcttttc tttcagcata 960 atccaaatga tagcatatgg ggtcccatgc attggggtca tgctgtcagt aaagatatga 1020 ttgaatggga agaactggat attgcattat atccagatga aaatgggaca atcttttctg 1080 gaagtgttgt gattgattgg aataacactt ccggtttttt tcctaaagag ccggggatgg 1140 tagcgatttt cactcaaaat ttacatgatg cggaccatac tacacctatt caaacacaaa 1200 gtttggcatt cagtcacgat cgaggcagaa cttggactaa atacgaaaag aatcctgtac 1260 ttaaacatcc aacgaaagtt gatttcaggg atcctaaagt gttttggcat tacgaaagtg 1320 aaaagtggat tatggtatta gctacaggcc aaactgtttc catttattcc tctcctaatt 1380 taatagattg gcaattcgaa agtgaatttg gagagaatat tggttgccat gatggtgtgt 1440 gggaatgtcc agatttattc gagttaccag ttgaaaatag cgaggaaaag aaatgggtta 1500 tgtttgtgag tattggagat aatcgtcaat tcgatagtgg ttcaagaaca cagtatttta 1560 ttggttcttt tgatgggtca aatttcaagg ttgatgaaaa taacaatgaa gtgctttggt 1620 tggatttcgg aaaagataat tacgctggtg taactttttc agatatccct aaagaagatg 1680 gcaggagaat ctatattggt tggatgagca attggagata tgctaatgag gttccaactg 1740 taggttggag aggccagatg accttaccaa gagttctttc tttaagaaaa attggagaca 1800 agctaagggt tatccaaaac ccagtaaaag aactagataa ctattttact aaacagattg 1860 taatccatga tgaaataatt agtagtggca tacaaaaaaa gattgatatc aatcaaccca 1920 gtgtagaaat tgtcttaaat gttaataatc ttggtgcgaa acaatttgga ttagtattgc 1980 atcacacaga aaaccaaagc acgacgatta caattgattt gttaacaaat ttattatcgt 2040 tggatagaac aaattctggt gaggttagct tttcagaaaa ctttttaaat aatcaagtta 2100 tgaaaattca tgatacgacg aaacttaatt tacgaataat tgtggattcc tcttctgtgg 2160 aaatatttgt gaatgaggga gcccatgcat tgaccagctt aatttatccg gataaagcat 2220 gtgaaaaaat atcgattttc tccttaggtg gagatataca actaagcaac agctacatat 2280 catttcctaa ctcaataaga agaaattttg ggtattgaaa ggagaaaatc tagtgcttgg 2340 tgcaatcgaa gcaggtggaa ctaaatttgt ctgtgctgtc ggcgatatgg attataacat 2400 tgttgattta attgaaatac cgactacttc tccaattgaa acaatggaga aagtaaaaga 2460 attttttatt cagtacaata ttcaatcatt aggaataggt tcatttggac cagctgatat 2520 taatcctagt tctccaaact acggttgtat cacaaatacg ccgaaagaac aatggaaaca 2580 tttcaatatc atatctttta tgaaaagtac attcaatgtt cccatagtct ttgatacaga 2640 tgtgaatgtt gcagcattgg gggaagcaaa gtttggagct tccagaaatg taaatagctc 2700 aatttatgta acaattggta ctggaattgg tgtgggagca gtcattaatg gggaaatatt 2760 acaaggaatt agccatcctg gagatgggtc acatatttgt tagaagacac gaaaaagatc 2820 attttgcagg atgctgtcct taccaccaag attgcttaga agggttagct tctggtccgg 2880 caattgaaaa aagatggggt aaaaaagctg ttttgcttga aaataataaa gaagtttggg 2940 aaatagaagg ctattatata gcacaagcat taatgaccta tattctaatt ttatctccgg 3000 aaaaaattat tcttggcgga ggagtcatga agcagagaca actgattact attatttatg 3060 aatacttaaa aagattaaat aacggttata tgtctttctc acagctagaa gaaaatattt 3120 cagattacat tgttacacct gaactagaag ggctttcggc tattaaaggt gcattctatt 3180 tatcaaaaaa taatttatat ggtaatacag aattattatc taaataattg tagaaaccat 3240 tcttttgatg ggcaatgata aatttgtaat ttaaatagat atctaggtta ataatgaaat 3300 ttattaaaac agagtttata aatgttgttg aatattaata gtttagaaag tatacaatag 3360 aaagtaaaag tgctgggctt tcctcaacat agagccagca tttttctttt attaaaattt 3420 actcaggtca aaagataaag ataaataagt tcaatctaat tattcaatat ctctcctgtt 3480 actcatcaca taatctacat tatatgacga accatccatt cttttttatg atatactcgt 3540 catataatgt tatttttcat atccgttatt tttgaaaaaa atcgttttta actcgccgta 3600 taagtgaaag ggtgaaaaag atgctcaacg agtatgtcgt ttacttggca agaaaaaggt 3660 gcttccaaaa ataccattat ttcctatacg aacgatttaa acatattttt tcatgattta 3720 catatccgtc cagatgatta tgtcacgccg gctgacatcc gcaaatggat ccaacacatg 3780 ttgaatccgg cagaaggaaa accgctggcg atctccacga tcaaccgccg cctcaattca 3840 ctgcgcagct tttatgcgtg ggcggttgag cgccattaca tcgagcaaaa tccgatgaag 3900 gatattcaag atttgaaaac ggctgatgaa gaccacgaaa aaatcatgtg gcttaccgaa 3960 gaagaattc 3969 // |
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